ES2046118A1 - Procedimiento mejorado para la produccion de glicosiltransferasas. - Google Patents

Procedimiento mejorado para la produccion de glicosiltransferasas.

Info

Publication number
ES2046118A1
ES2046118A1 ES9201117A ES9201117A ES2046118A1 ES 2046118 A1 ES2046118 A1 ES 2046118A1 ES 9201117 A ES9201117 A ES 9201117A ES 9201117 A ES9201117 A ES 9201117A ES 2046118 A1 ES2046118 A1 ES 2046118A1
Authority
ES
Spain
Prior art keywords
glycosyltransferase
promoter
variant
sequence
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES9201117A
Other languages
English (en)
Other versions
ES2046118B1 (es
Inventor
Gabriele Watzele
Eric G Berger
Bernd Meyhack
Manfred Watzele
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novartis AG
Original Assignee
Ciba Geigy AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB9208211A external-priority patent/GB2256197B/en
Application filed by Ciba Geigy AG filed Critical Ciba Geigy AG
Publication of ES2046118A1 publication Critical patent/ES2046118A1/es
Application granted granted Critical
Publication of ES2046118B1 publication Critical patent/ES2046118B1/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PROCEDIMIENTO MEJORADO PARA LA PRODUCCION DE GLICOSILTRANSFERASAS LIGADAS A MEMBRANAS Y SELECCIONADAS DEL GRUPO CONSISTENTE EN UNA GALACTOSILTRANSFERASA, UNA SIALILTRANSFERASA Y UNA FUCOSILTRANSFERASA, O UNA VARIANTE DE LAS MISMAS, RESPECTIVAMENTE. COMPRENDE CULTIVAR UNA CEPA DE LEVADURA QUE HA SIDO TRANSFORMADA CON UN VECTOR HIBRIDO QUE COMPRENDE UNA CASSETTE DE EXPRESION QUE INCLUYE UN PROMOTOR, UNA SECUENCIA DNA CODIFICADORA DE DICHA GLICOSILTRANSFERASA O VARIANTE, CUYA SECUENCIA DNA ES CONTROLADA POR DICHO PROMOTOR, Y UNA SECUENCIA DNA QUE CONTIENE SEÑALES DE TERMINACION DE LA TRANSCRIPCION DE LEVADURA; Y RECUPERAR LA ACTIVIDAD ENZIMATICA.
ES09201117A 1991-05-31 1992-05-29 Procedimiento mejorado para la produccion de glicosiltransferasas. Expired - Fee Related ES2046118B1 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP91810414 1991-05-31
EP92810167 1992-03-04
GB9208211A GB2256197B (en) 1991-05-31 1992-04-14 Yeast as host for expression of heterologous glycosyl transferase enzymes

Publications (2)

Publication Number Publication Date
ES2046118A1 true ES2046118A1 (es) 1994-01-16
ES2046118B1 ES2046118B1 (es) 1994-08-16

Family

ID=27234292

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09201117A Expired - Fee Related ES2046118B1 (es) 1991-05-31 1992-05-29 Procedimiento mejorado para la produccion de glicosiltransferasas.

Country Status (19)

Country Link
JP (1) JPH05199871A (es)
AT (1) AT401940B (es)
AU (1) AU655470B2 (es)
BE (1) BE1005579A5 (es)
CA (1) CA2070057A1 (es)
DK (1) DK71992A (es)
ES (1) ES2046118B1 (es)
FI (1) FI922515A (es)
GR (1) GR920100250A (es)
HU (1) HU212927B (es)
IE (1) IE69059B1 (es)
IL (1) IL102052A0 (es)
IT (1) IT1255044B (es)
LU (1) LU88123A1 (es)
MX (1) MX9202583A (es)
NO (1) NO922134L (es)
NZ (1) NZ242958A (es)
PT (1) PT100545A (es)
SE (1) SE9201544L (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE7819584U1 (de) * 1978-06-30 1978-10-12 Howmedica International, Inc. Zweigniederlassung Kiel, 2301 Schoenkirchen Tubenartiger vorratsbehaelter fuer medizinisches spritzgeraet
HU219260B (en) * 1992-03-09 2001-03-28 Cytel Corp Recombinant polypeptid with sialyltransferase activity, dna molecule coding thereof and vectors
AU4858801A (en) * 2000-04-13 2001-10-30 Biotica Tech Ltd Hybrid glycosylated products and their production and use
US7173003B2 (en) 2001-10-10 2007-02-06 Neose Technologies, Inc. Granulocyte colony stimulating factor: remodeling and glycoconjugation of G-CSF
US7214660B2 (en) 2001-10-10 2007-05-08 Neose Technologies, Inc. Erythropoietin: remodeling and glycoconjugation of erythropoietin
EP2338333B1 (en) 2003-04-09 2017-09-06 ratiopharm GmbH Glycopegylation methods and proteins/peptides produced by the methods
WO2005012484A2 (en) 2003-07-25 2005-02-10 Neose Technologies, Inc. Antibody-toxin conjugates
US20080305992A1 (en) 2003-11-24 2008-12-11 Neose Technologies, Inc. Glycopegylated erythropoietin
WO2006010143A2 (en) 2004-07-13 2006-01-26 Neose Technologies, Inc. Branched peg remodeling and glycosylation of glucagon-like peptide-1 [glp-1]
SI2586456T1 (sl) 2004-10-29 2016-05-31 Ratiopharm Gmbh Preoblikovanje in glikopegliacija fibroblastnega rastnega faktorja (FGF)
EP1858543B1 (en) 2005-01-10 2013-11-27 BioGeneriX AG Glycopegylated granulocyte colony stimulating factor
WO2006121569A2 (en) 2005-04-08 2006-11-16 Neose Technologies, Inc. Compositions and methods for the preparation of protease resistant human growth hormone glycosylation mutants
US20070105755A1 (en) 2005-10-26 2007-05-10 Neose Technologies, Inc. One pot desialylation and glycopegylation of therapeutic peptides
US20090048440A1 (en) 2005-11-03 2009-02-19 Neose Technologies, Inc. Nucleotide Sugar Purification Using Membranes
CN101516388B (zh) 2006-07-21 2012-10-31 诺和诺德公司 通过o-联糖基化序列的肽的糖基化
EP2054521A4 (en) 2006-10-03 2012-12-19 Novo Nordisk As METHODS OF PURIFYING CONJUGATES OF POLYPEPTIDES
ES2406267T3 (es) 2007-04-03 2013-06-06 Biogenerix Ag Métodos de tratamiento usando G-CSF glicopegilado
WO2008154639A2 (en) 2007-06-12 2008-12-18 Neose Technologies, Inc. Improved process for the production of nucleotide sugars
PL2257311T3 (pl) 2008-02-27 2014-09-30 Novo Nordisk As Koniugaty cząsteczek czynnika VIII

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990007000A2 (en) * 1988-12-13 1990-06-28 La Jolla Cancer Research Foundation Nucleotides encoding human b1, 4-galactosyltransferase and uses thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MY103358A (en) * 1987-04-15 1993-06-30 Novartis Ag Process for the production of protiens.
US5032519A (en) * 1989-10-24 1991-07-16 The Regents Of The Univ. Of California Method for producing secretable glycosyltransferases and other Golgi processing enzymes
CA2075949A1 (en) * 1990-02-14 1991-08-15 John B. Lowe Methods and products for the synthesis of oligosaccharide structures on glycoproteins, glycolipids, or as free molecules
JPH05501359A (ja) * 1990-04-27 1993-03-18 バイオジェン，インコーポレイテッド 付着分子発現に関与するフコシルトランスフェラーゼ
DE4028800A1 (de) * 1990-09-11 1992-03-12 Behringwerke Ag Gentechnische sialylierung von glykoproteinen

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1990007000A2 (en) * 1988-12-13 1990-06-28 La Jolla Cancer Research Foundation Nucleotides encoding human b1, 4-galactosyltransferase and uses thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
J. BIOL. CHEM. Vol. 265 n‘ 11, pßg. 6225-6234 (1990) DAVID F. SMITH et al. *
J. BIOL. CHEM. Vol. 265, pßg. 1615-1622, (1990) BARRY POTVIN et al. *
J. CELL BIOL. Vol. 107, 10A (1988) J.C. PAULSON et al. * Resumen * *
YEAST BIOTECHNOLOGY, pßg. 401-429, Ed. D.R. BERRY, J. RUSELL, G.G. STEWART, ALLEN AND UNWIN (1987) A.R. GOODEY et al. *

Also Published As

Publication number Publication date
LU88123A1 (de) 1993-12-06
ES2046118B1 (es) 1994-08-16
NO922134L (no) 1992-12-01
GR920100250A (el) 1993-03-31
AT401940B (de) 1996-12-27
ITRM920411A0 (it) 1992-05-29
ITRM920411A1 (it) 1993-11-29
SE9201544L (sv) 1992-12-01
HU212927B (en) 1996-12-30
FI922515A (fi) 1992-12-01
PT100545A (pt) 1993-08-31
IT1255044B (it) 1995-10-17
CA2070057A1 (en) 1992-12-01
BE1005579A5 (fr) 1993-11-09
NO922134D0 (no) 1992-05-29
ATA112792A (de) 1996-05-15
HUT65728A (en) 1994-07-28
FI922515A0 (fi) 1992-05-29
NZ242958A (en) 1993-06-25
MX9202583A (es) 1992-11-01
DK71992D0 (da) 1992-05-29
IL102052A0 (en) 1992-12-30
IE921769A1 (en) 1992-12-02
DK71992A (da) 1992-12-01
HU9201787D0 (en) 1992-08-28
SE9201544D0 (sv) 1992-05-15
AU655470B2 (en) 1994-12-22
AU1705292A (en) 1992-12-03
JPH05199871A (ja) 1993-08-10
IE69059B1 (en) 1996-08-07

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 20041102