EP3480209A3 - Expressionssystem - Google Patents

Expressionssystem Download PDF

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Publication number
EP3480209A3
EP3480209A3 EP18167370.8A EP18167370A EP3480209A3 EP 3480209 A3 EP3480209 A3 EP 3480209A3 EP 18167370 A EP18167370 A EP 18167370A EP 3480209 A3 EP3480209 A3 EP 3480209A3
Authority
EP
European Patent Office
Prior art keywords
expression system
toxin
crm197
compositions
methods related
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP18167370.8A
Other languages
English (en)
French (fr)
Other versions
EP3480209A2 (de
EP3480209B1 (de
Inventor
Normand Blais
Philippe Marc Helene Dehottay
Marianne Dewerchin
Philippe Goffin
Denis Martin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GlaxoSmithKline Biologicals SA
Original Assignee
GlaxoSmithKline Biologicals SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=41402733&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EP3480209(A3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by GlaxoSmithKline Biologicals SA filed Critical GlaxoSmithKline Biologicals SA
Publication of EP3480209A2 publication Critical patent/EP3480209A2/de
Publication of EP3480209A3 publication Critical patent/EP3480209A3/de
Application granted granted Critical
Publication of EP3480209B1 publication Critical patent/EP3480209B1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/02—Bacterial antigens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1077—Pentosyltransferases (2.4.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/02—Pentosyltransferases (2.4.2)
    • C12Y204/02036—NAD(+)--diphthamide ADP-ribosyltransferase (2.4.2.36)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Public Health (AREA)
  • Physics & Mathematics (AREA)
  • Veterinary Medicine (AREA)
  • Plant Pathology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Analytical Chemistry (AREA)
  • Mycology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Oncology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Communicable Diseases (AREA)
  • Epidemiology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
EP18167370.8A 2009-10-08 2010-10-07 Expressionssystem Active EP3480209B1 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB0917647.0A GB0917647D0 (en) 2009-10-08 2009-10-08 Expression system
PCT/EP2010/065047 WO2011042516A2 (en) 2009-10-08 2010-10-07 Expression system
EP10762693.9A EP2486047B1 (de) 2009-10-08 2010-10-07 Expressionssystem
EP16192258.8A EP3170837B1 (de) 2009-10-08 2010-10-07 Expressionssystem

Related Parent Applications (3)

Application Number Title Priority Date Filing Date
EP10762693.9A Division EP2486047B1 (de) 2009-10-08 2010-10-07 Expressionssystem
EP16192258.8A Division EP3170837B1 (de) 2009-10-08 2010-10-07 Expressionssystem
EP16192258.8A Division-Into EP3170837B1 (de) 2009-10-08 2010-10-07 Expressionssystem

Publications (3)

Publication Number Publication Date
EP3480209A2 EP3480209A2 (de) 2019-05-08
EP3480209A3 true EP3480209A3 (de) 2019-06-19
EP3480209B1 EP3480209B1 (de) 2023-11-29

Family

ID=41402733

Family Applications (3)

Application Number Title Priority Date Filing Date
EP10762693.9A Active EP2486047B1 (de) 2009-10-08 2010-10-07 Expressionssystem
EP16192258.8A Active EP3170837B1 (de) 2009-10-08 2010-10-07 Expressionssystem
EP18167370.8A Active EP3480209B1 (de) 2009-10-08 2010-10-07 Expressionssystem

Family Applications Before (2)

Application Number Title Priority Date Filing Date
EP10762693.9A Active EP2486047B1 (de) 2009-10-08 2010-10-07 Expressionssystem
EP16192258.8A Active EP3170837B1 (de) 2009-10-08 2010-10-07 Expressionssystem

Country Status (17)

Country Link
US (4) US9422345B2 (de)
EP (3) EP2486047B1 (de)
JP (3) JP2013507113A (de)
KR (1) KR20120099672A (de)
CN (1) CN102639558B (de)
AR (1) AR078533A1 (de)
AU (1) AU2010305342B2 (de)
BR (1) BR112012007984B1 (de)
CA (1) CA2776969C (de)
EA (1) EA201290134A1 (de)
ES (2) ES2750023T3 (de)
GB (1) GB0917647D0 (de)
IL (1) IL218930A0 (de)
MX (2) MX2012004092A (de)
SG (1) SG10201405940VA (de)
UY (1) UY32933A (de)
WO (1) WO2011042516A2 (de)

Families Citing this family (32)

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MX339524B (es) 2001-10-11 2016-05-30 Wyeth Corp Composiciones inmunogenicas novedosas para la prevencion y tratamiento de enfermedad meningococica.
GB0917647D0 (en) * 2009-10-08 2009-11-25 Glaxosmithkline Biolog Sa Expression system
NZ602958A (en) 2010-03-30 2014-07-25 Pfenex Inc High level expression of recombinant toxin proteins
WO2012025873A2 (en) 2010-08-23 2012-03-01 Wyeth Llc STABLE FORMULATIONS OF NEISSERIA MENINGITIDIS rLP2086 ANTIGENS
PE20140173A1 (es) 2010-09-10 2014-02-20 Wyeth Llc Variantes no lipidadas de antigenos orf2086 de neisseria meningitidis
GB201106225D0 (en) * 2011-04-13 2011-05-25 Glaxosmithkline Biolog Sa Fermentation process
SG10201602558UA (en) 2012-03-09 2016-05-30 Pfizer Neisseria meningitidis compositions and methods thereof
SA115360586B1 (ar) 2012-03-09 2017-04-12 فايزر انك تركيبات لعلاج الالتهاب السحائي البكتيري وطرق لتحضيرها
US10287330B2 (en) 2012-12-27 2019-05-14 Glaxosmithkline Biologicals S.A. Methods and compositions relating to CRM197
EP2964665B1 (de) 2013-03-08 2018-08-01 Pfizer Inc Immunogene fusionspolypeptide
CA2923129C (en) 2013-09-08 2020-06-09 Pfizer Inc. Neisseria meningitidis compositions and methods thereof
US11060123B2 (en) 2014-01-31 2021-07-13 Fina Biosolutions, Llc Production of soluble recombinant protein without n-terminal methionine
US10093704B2 (en) 2014-01-31 2018-10-09 Fina Biosolutions, Llc Expression and purification of CRM197 and related proteins
US10479820B2 (en) 2014-03-03 2019-11-19 Scarab Genomics, Llc Enhanced production of recombinant CRM197 in E. coli
MA39746A (fr) 2014-03-14 2021-04-28 Hoffmann La Roche Compositions de sécrétion de polypeptides hétérologues et procédés associés
EP3221339A1 (de) * 2014-11-20 2017-09-27 Biological E Limited Codon-optimiertes polynukleotid für hochgradige expression von crm197
BR112017017460A2 (pt) 2015-02-19 2018-04-10 Pfizer Inc. composições de neisseria meningitidis e métodos das mesmas
US11951165B2 (en) 2016-12-30 2024-04-09 Vaxcyte, Inc. Conjugated vaccine carrier proteins
AU2018215585B2 (en) 2017-01-31 2022-03-17 Pfizer Inc. Neisseria meningitidis compositions and methods thereof
KR101908590B1 (ko) * 2017-02-01 2018-10-16 (주)포바이오코리아 Crm197의 용해성 단백질 발현 및 정제 방법
EP3444269A1 (de) 2017-08-17 2019-02-20 National Research Council of Canada Systeme und methoden für die produktion von diphteria-giftstoff-polypeptiden
WO2019043593A1 (en) * 2017-09-01 2019-03-07 Lorven Biologics Private Limited NUCLEIC ACID ENCODING CRM197 AND ENHANCED EXPRESSION PROCESS THEREOF
CA3074718A1 (en) 2017-09-05 2019-03-14 Scarab Genomics, Llc Methods and compositions for improved expression of recombinant proteins
CN111278852A (zh) 2017-10-27 2020-06-12 菲尼克斯公司 重组欧氏杆菌天冬酰胺酶的生产方法
JP2021501157A (ja) * 2017-10-27 2021-01-14 フェネックス インク． 周辺タンパク質発現のための細菌リーダー配列
CA3084436A1 (en) 2017-12-06 2019-07-18 Merck Sharp & Dohme Corp. Compositions comprising streptococcus pneumoniae polysaccharide-protein conjugates and methods of use thereof
KR102059619B1 (ko) 2018-04-17 2019-12-26 강원대학교산학협력단 글루코스-자일로스 혼합당의 동시발효가 가능한 돌연변이 균주
WO2020033398A1 (en) * 2018-08-06 2020-02-13 The Trustees Of Columbia University In The City Of New York Compositions and methods for treating atii cell-dependent lung diseases
AU2019401535B2 (en) 2018-12-19 2023-12-14 Merck Sharp & Dohme Llc Compositions comprising Streptococcus pneumoniae polysaccharide-protein conjugates and methods of use thereof
EP3942079A1 (de) 2019-03-18 2022-01-26 Bio-Rad ABD Serotec GmbH Schutz von spytag-haltigen periplasmatischen fusionsproteinen vor protease-tsp- und ompt-abbau
KR102099342B1 (ko) * 2019-09-03 2020-04-10 주식회사 제노포커스 Crm197 단백질 발현 방법
BE1029145B1 (fr) 2021-02-26 2022-09-27 Curavac Europe Methode de production d'une forme periplasmique de la proteine crm197

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Also Published As

Publication number Publication date
JP2017029155A (ja) 2017-02-09
US20180258146A1 (en) 2018-09-13
EA201290134A1 (ru) 2012-10-30
AR078533A1 (es) 2011-11-16
JP2018085994A (ja) 2018-06-07
SG10201405940VA (en) 2014-11-27
AU2010305342A1 (en) 2012-05-03
EP2486047A2 (de) 2012-08-15
IL218930A0 (en) 2012-07-31
CN102639558A (zh) 2012-08-15
CA2776969A1 (en) 2011-04-14
BR112012007984A2 (pt) 2016-11-22
JP2013507113A (ja) 2013-03-04
MX347256B (es) 2017-04-20
CA2776969C (en) 2019-01-15
UY32933A (es) 2011-05-31
WO2011042516A3 (en) 2011-06-16
EP2486047B1 (de) 2016-10-05
US20120289688A1 (en) 2012-11-15
AU2010305342B2 (en) 2016-05-26
EP3170837A3 (de) 2017-07-19
JP6305478B2 (ja) 2018-04-04
US9422345B2 (en) 2016-08-23
CN102639558B (zh) 2015-11-25
EP3480209A2 (de) 2019-05-08
MX2012004092A (es) 2012-04-20
EP3170837A2 (de) 2017-05-24
US20190322708A1 (en) 2019-10-24
EP3480209B1 (de) 2023-11-29
EP3170837B1 (de) 2019-08-21
ES2750023T3 (es) 2020-03-24
WO2011042516A2 (en) 2011-04-14
BR112012007984B1 (pt) 2021-11-03
GB0917647D0 (en) 2009-11-25
US20170137475A1 (en) 2017-05-18
JP6636546B2 (ja) 2020-01-29
US9994622B2 (en) 2018-06-12
ES2609066T3 (es) 2017-04-18
KR20120099672A (ko) 2012-09-11

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