ATE448305T1 - Phagen-display mittels cotranslationaler translokation von fusionspolypeptiden - Google Patents

Phagen-display mittels cotranslationaler translokation von fusionspolypeptiden

Info

Publication number
ATE448305T1
ATE448305T1 AT06763985T AT06763985T ATE448305T1 AT E448305 T1 ATE448305 T1 AT E448305T1 AT 06763985 T AT06763985 T AT 06763985T AT 06763985 T AT06763985 T AT 06763985T AT E448305 T1 ATE448305 T1 AT E448305T1
Authority
AT
Austria
Prior art keywords
phage
polypeptides
phage display
particle
cotranslational translocation
Prior art date
Application number
AT06763985T
Other languages
English (en)
Inventor
Daniel Steiner
Patrik Forrer
Michael Stumpp
Andreas Plueckthun
Original Assignee
Univ Zuerich
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Zuerich filed Critical Univ Zuerich
Application granted granted Critical
Publication of ATE448305T1 publication Critical patent/ATE448305T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
    • C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
AT06763985T 2005-07-08 2006-06-30 Phagen-display mittels cotranslationaler translokation von fusionspolypeptiden ATE448305T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP05106236 2005-07-08
PCT/EP2006/063729 WO2007006665A1 (en) 2005-07-08 2006-06-30 Phage display using cotranslational translocation of fusion polypeptides

Publications (1)

Publication Number Publication Date
ATE448305T1 true ATE448305T1 (de) 2009-11-15

Family

ID=35063135

Family Applications (1)

Application Number Title Priority Date Filing Date
AT06763985T ATE448305T1 (de) 2005-07-08 2006-06-30 Phagen-display mittels cotranslationaler translokation von fusionspolypeptiden

Country Status (21)

Country Link
US (2) US8846577B2 (de)
EP (1) EP1902131B1 (de)
JP (1) JP5042218B2 (de)
KR (1) KR101268939B1 (de)
CN (1) CN101213299B (de)
AT (1) ATE448305T1 (de)
AU (1) AU2006268780B2 (de)
BR (1) BRPI0613593A2 (de)
CA (1) CA2614512C (de)
DE (1) DE602006010386D1 (de)
DK (1) DK1902131T3 (de)
ES (1) ES2335136T3 (de)
HK (1) HK1122839A1 (de)
IL (1) IL188518A (de)
MX (1) MX2008000345A (de)
NO (1) NO20080665L (de)
NZ (1) NZ564695A (de)
PL (1) PL1902131T3 (de)
RU (1) RU2412249C2 (de)
WO (1) WO2007006665A1 (de)
ZA (1) ZA200710687B (de)

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US7618799B2 (en) * 2007-01-31 2009-11-17 Dow Global Technologies Inc Bacterial leader sequences for increased expression
WO2010060748A1 (en) 2008-11-03 2010-06-03 Molecular Partners Ag Binding proteins inhibiting the vegf-a receptor interaction
GB0917647D0 (en) * 2009-10-08 2009-11-25 Glaxosmithkline Biolog Sa Expression system
EP2496601B1 (de) * 2009-11-05 2017-06-07 F. Hoffmann-La Roche AG Methoden und zusammensetzung zur sekretion heterologer polypeptide
AR081361A1 (es) 2010-04-30 2012-08-29 Molecular Partners Ag Proteinas de union modificadas que inhiben la interaccion de receptor del factor de crecimiento endotelial vascular de glicoproteina a vegf-a
ES2758352T3 (es) 2010-11-26 2020-05-05 Molecular Partners Ag Proteínas de repetición diseñadas que se une a la seroalbúmina
RU2748733C2 (ru) 2011-02-15 2021-05-31 Иммуноджен, Инк. Цитотоксические производные бензодиазепина
TW201302779A (zh) * 2011-04-13 2013-01-16 Glaxosmithkline Biolog Sa 融合蛋白質及組合疫苗
AU2012249359A1 (en) 2011-04-29 2013-11-14 Janssen Biotech, Inc. IL4/IL13 binding repeat proteins and uses
US9289512B2 (en) 2011-06-21 2016-03-22 Immunogen, Inc. Maytansinoid derivatives with peptide linker and conjugates thereof
AU2013283296A1 (en) 2012-06-28 2015-02-05 Molecular Partners Ag Designed ankyrin repeat proteins binding to platelet-derived growth factor
US9956341B2 (en) 2012-07-03 2018-05-01 Milestone Scientific, Inc. Drug infusion with pressure sensing and non-continuous flow for identification of and injection into fluid-filled anatomic spaces
EP2887965A1 (de) 2012-08-22 2015-07-01 ImmunoGen, Inc. Zytotoxische benzodiazepin-derivate
EP2738180A1 (de) 2012-11-30 2014-06-04 Molecular Partners AG Bindeproteine mit mindestens zwei Bindedomänen gegen HER2
JP6423804B2 (ja) 2013-02-28 2018-11-14 イミュノジェン・インコーポレーテッド 細胞結合剤及び細胞毒性剤を含む複合体
EP2961434A2 (de) 2013-02-28 2016-01-06 ImmunoGen, Inc. Konjugate mit zellbindenden mitteln und zytostatika
WO2014194030A2 (en) 2013-05-31 2014-12-04 Immunogen, Inc. Conjugates comprising cell-binding agents and cytotoxic agents
EP3004152B1 (de) 2013-05-31 2020-09-30 Molecular Partners AG An den hepatozytenwachstumsfaktor bindende, konstruierte ankyrin-repeat-proteine
CA2941687A1 (en) 2014-03-14 2015-09-17 Genentech, Inc. Methods and compositions for secretion of heterologous polypeptides
RU2016141385A (ru) 2014-03-24 2018-04-28 Дженентек, Инк. Лечение рака антагонистами с-мет и их корреляция с экспрессией hgf
WO2016036804A1 (en) 2014-09-03 2016-03-10 Immunogen, Inc. Cytotoxic benzodiazepine derivatives
TWI697493B (zh) 2014-09-03 2020-07-01 美商免疫原公司 細胞毒性苯并二氮呯衍生物
BR112017020986A2 (pt) 2015-04-02 2018-08-14 Molecular Partners Ag. proteínas de ligação recombinantes e seu uso
US10220180B2 (en) 2015-10-16 2019-03-05 Milestone Scientific, Inc. Method and apparatus for performing a peripheral nerve block
MA46111A (fr) 2016-08-31 2019-07-10 Harvard College Bactéries génétiquement modifiées sécrétant des protéines thérapeutiques et procédés d'utilisation de celles-ci
US10717772B2 (en) 2016-09-22 2020-07-21 Molecular Partners Ag Recombinant binding proteins targeting HER2 and serum albumin, and their uses
US10632255B2 (en) 2017-02-15 2020-04-28 Milestone Scientific, Inc. Drug infusion device
CN116785450A (zh) 2017-02-28 2023-09-22 伊缪诺金公司 具有自分解肽接头的类美登素衍生物和其缀合物
TW201839001A (zh) 2017-04-20 2018-11-01 美商伊繆諾金公司 細胞毒性苯并二氮平衍生物及其綴合物
US11471595B2 (en) 2017-05-04 2022-10-18 Milestone Scientific, Inc. Method and apparatus for performing a peripheral nerve block
WO2019133652A1 (en) 2017-12-28 2019-07-04 Immunogen, Inc. Benzodiazepine derivatives
CN113631194A (zh) 2019-03-21 2021-11-09 伊缪诺金公司 制备细胞结合剂-药物缀合物的方法
JP2022529583A (ja) 2019-03-29 2022-06-23 イミュノジェン・インコーポレーテッド 異常細胞増殖を阻害するまたは増殖性疾患を治療するための細胞毒性ビス－ベンゾジアゼピン誘導体及び細胞結合剤とのその複合体
FI3958977T3 (fi) 2019-04-26 2023-12-12 Immunogen Inc Kamptotesiinijohdannaisia
US10646660B1 (en) 2019-05-16 2020-05-12 Milestone Scientific, Inc. Device and method for identification of a target region
MX2022007122A (es) 2019-12-11 2022-09-09 Molecular Partners Ag Dominios de repetición de anquirina diseñados con residuos de superficie alterados.
CA3176845A1 (en) 2020-05-06 2021-11-11 Patrick AMSTUTZ Novel ankyrin repeat binding proteins and their uses
WO2022190016A1 (en) 2021-03-09 2022-09-15 Molecular Partners Ag Novel darpin based multi-specific t-cell engagers

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Publication number Priority date Publication date Assignee Title
EP1078051B1 (de) 1998-05-13 2007-12-12 Domantis Limited Phagen-display-selektionssystem für korrekt gefaltete proteine

Also Published As

Publication number Publication date
WO2007006665A1 (en) 2007-01-18
KR101268939B1 (ko) 2013-05-30
RU2008104174A (ru) 2009-08-20
US9447142B2 (en) 2016-09-20
JP5042218B2 (ja) 2012-10-03
US20110118146A1 (en) 2011-05-19
AU2006268780B2 (en) 2011-03-24
MX2008000345A (es) 2008-04-02
DE602006010386D1 (en) 2009-12-24
PL1902131T3 (pl) 2010-05-31
RU2412249C2 (ru) 2011-02-20
DK1902131T3 (da) 2010-03-01
ES2335136T3 (es) 2010-03-22
KR20080035603A (ko) 2008-04-23
NO20080665L (no) 2008-02-05
EP1902131A1 (de) 2008-03-26
ZA200710687B (en) 2008-11-26
US8846577B2 (en) 2014-09-30
EP1902131B1 (de) 2009-11-11
AU2006268780A1 (en) 2007-01-18
CN101213299A (zh) 2008-07-02
IL188518A0 (en) 2008-04-13
JP2009500027A (ja) 2009-01-08
HK1122839A1 (en) 2009-05-29
BRPI0613593A2 (pt) 2011-01-18
IL188518A (en) 2011-04-28
NZ564695A (en) 2009-02-28
CA2614512C (en) 2014-01-07
CA2614512A1 (en) 2007-01-18
US20150057186A1 (en) 2015-02-26
CN101213299B (zh) 2013-03-27

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