NO20080665L - Fag-fremvisning i a benytte kontraslasjonell translokasjon av fusjonspolypeptider - Google Patents
Fag-fremvisning i a benytte kontraslasjonell translokasjon av fusjonspolypeptiderInfo
- Publication number
- NO20080665L NO20080665L NO20080665A NO20080665A NO20080665L NO 20080665 L NO20080665 L NO 20080665L NO 20080665 A NO20080665 A NO 20080665A NO 20080665 A NO20080665 A NO 20080665A NO 20080665 L NO20080665 L NO 20080665L
- Authority
- NO
- Norway
- Prior art keywords
- phage
- translocation
- polypeptides
- phage display
- contralateral
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
- C07K1/047—Simultaneous synthesis of different peptide species; Peptide libraries
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/40—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
- C07K2319/43—Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a FLAG-tag
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/50—Fusion polypeptide containing protease site
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
Abstract
Den foreliggende oppfinnelse er relatert til en fremgangsmåte av filamentøs fagfremvisning for polypeptidene av interesse fremvist på fagpartikkelen er kotranslasjonell translokert over den cytoplasmiske membranen av gramnegative bakterier basert på signalgjenkjermings partikkelvelen. Derme fremgangsmåten er spesielt egnet for polypeptider, som er kjent til å være vanskelig å fremvise på fag, og for proteiner av cDNA bibliotek og andre kombinasjonsmessige bibliotek, spesielt når derivert fiia veldig raskt foldende, stabile proteinskaffblder. Oppfirmelsen er videre relatert til fag eller fagmidvektorer nyttige i fremgangsmåten omfattende en genkonstruksjon kodende for et fusjonspolypeptid omfattende polypeptidet som skal bli fremvist på fagpartikkelen og en N-terminal signalsekvens som fremmer kotranslasjonell translokasjon.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP05106236 | 2005-07-08 | ||
PCT/EP2006/063729 WO2007006665A1 (en) | 2005-07-08 | 2006-06-30 | Phage display using cotranslational translocation of fusion polypeptides |
Publications (1)
Publication Number | Publication Date |
---|---|
NO20080665L true NO20080665L (no) | 2008-02-05 |
Family
ID=35063135
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO20080665A NO20080665L (no) | 2005-07-08 | 2008-02-05 | Fag-fremvisning i a benytte kontraslasjonell translokasjon av fusjonspolypeptider |
Country Status (21)
Country | Link |
---|---|
US (2) | US8846577B2 (no) |
EP (1) | EP1902131B1 (no) |
JP (1) | JP5042218B2 (no) |
KR (1) | KR101268939B1 (no) |
CN (1) | CN101213299B (no) |
AT (1) | ATE448305T1 (no) |
AU (1) | AU2006268780B2 (no) |
BR (1) | BRPI0613593A2 (no) |
CA (1) | CA2614512C (no) |
DE (1) | DE602006010386D1 (no) |
DK (1) | DK1902131T3 (no) |
ES (1) | ES2335136T3 (no) |
HK (1) | HK1122839A1 (no) |
IL (1) | IL188518A (no) |
MX (1) | MX2008000345A (no) |
NO (1) | NO20080665L (no) |
NZ (1) | NZ564695A (no) |
PL (1) | PL1902131T3 (no) |
RU (1) | RU2412249C2 (no) |
WO (1) | WO2007006665A1 (no) |
ZA (1) | ZA200710687B (no) |
Families Citing this family (39)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2490280A1 (en) | 2002-07-01 | 2004-01-08 | Human Genome Sciences, Inc. | Antibodies that specifically bind to reg iv |
US7618799B2 (en) * | 2007-01-31 | 2009-11-17 | Dow Global Technologies Inc | Bacterial leader sequences for increased expression |
WO2010060748A1 (en) | 2008-11-03 | 2010-06-03 | Molecular Partners Ag | Binding proteins inhibiting the vegf-a receptor interaction |
GB0917647D0 (en) * | 2009-10-08 | 2009-11-25 | Glaxosmithkline Biolog Sa | Expression system |
EP2496601B1 (en) * | 2009-11-05 | 2017-06-07 | F. Hoffmann-La Roche AG | Methods and composition for secretion of heterologous polypeptides |
AR081361A1 (es) | 2010-04-30 | 2012-08-29 | Molecular Partners Ag | Proteinas de union modificadas que inhiben la interaccion de receptor del factor de crecimiento endotelial vascular de glicoproteina a vegf-a |
ES2758352T3 (es) | 2010-11-26 | 2020-05-05 | Molecular Partners Ag | Proteínas de repetición diseñadas que se une a la seroalbúmina |
RU2748733C2 (ru) | 2011-02-15 | 2021-05-31 | Иммуноджен, Инк. | Цитотоксические производные бензодиазепина |
TW201302779A (zh) * | 2011-04-13 | 2013-01-16 | Glaxosmithkline Biolog Sa | 融合蛋白質及組合疫苗 |
AU2012249359A1 (en) | 2011-04-29 | 2013-11-14 | Janssen Biotech, Inc. | IL4/IL13 binding repeat proteins and uses |
US9289512B2 (en) | 2011-06-21 | 2016-03-22 | Immunogen, Inc. | Maytansinoid derivatives with peptide linker and conjugates thereof |
AU2013283296A1 (en) | 2012-06-28 | 2015-02-05 | Molecular Partners Ag | Designed ankyrin repeat proteins binding to platelet-derived growth factor |
US9956341B2 (en) | 2012-07-03 | 2018-05-01 | Milestone Scientific, Inc. | Drug infusion with pressure sensing and non-continuous flow for identification of and injection into fluid-filled anatomic spaces |
EP2887965A1 (en) | 2012-08-22 | 2015-07-01 | ImmunoGen, Inc. | Cytotoxic benzodiazepine derivatives |
EP2738180A1 (en) | 2012-11-30 | 2014-06-04 | Molecular Partners AG | Binding proteins comprising at least two binding domains against HER2. |
JP6423804B2 (ja) | 2013-02-28 | 2018-11-14 | イミュノジェン・インコーポレーテッド | 細胞結合剤及び細胞毒性剤を含む複合体 |
EP2961434A2 (en) | 2013-02-28 | 2016-01-06 | ImmunoGen, Inc. | Conjugates comprising cell-binding agents and cytotoxic agents |
WO2014194030A2 (en) | 2013-05-31 | 2014-12-04 | Immunogen, Inc. | Conjugates comprising cell-binding agents and cytotoxic agents |
EP3004152B1 (en) | 2013-05-31 | 2020-09-30 | Molecular Partners AG | Designed ankyrin repeat proteins binding to hepatocyte growth factor |
CA2941687A1 (en) | 2014-03-14 | 2015-09-17 | Genentech, Inc. | Methods and compositions for secretion of heterologous polypeptides |
RU2016141385A (ru) | 2014-03-24 | 2018-04-28 | Дженентек, Инк. | Лечение рака антагонистами с-мет и их корреляция с экспрессией hgf |
WO2016036804A1 (en) | 2014-09-03 | 2016-03-10 | Immunogen, Inc. | Cytotoxic benzodiazepine derivatives |
TWI697493B (zh) | 2014-09-03 | 2020-07-01 | 美商免疫原公司 | 細胞毒性苯并二氮呯衍生物 |
BR112017020986A2 (pt) | 2015-04-02 | 2018-08-14 | Molecular Partners Ag. | proteínas de ligação recombinantes e seu uso |
US10220180B2 (en) | 2015-10-16 | 2019-03-05 | Milestone Scientific, Inc. | Method and apparatus for performing a peripheral nerve block |
MA46111A (fr) | 2016-08-31 | 2019-07-10 | Harvard College | Bactéries génétiquement modifiées sécrétant des protéines thérapeutiques et procédés d'utilisation de celles-ci |
US10717772B2 (en) | 2016-09-22 | 2020-07-21 | Molecular Partners Ag | Recombinant binding proteins targeting HER2 and serum albumin, and their uses |
US10632255B2 (en) | 2017-02-15 | 2020-04-28 | Milestone Scientific, Inc. | Drug infusion device |
CN116785450A (zh) | 2017-02-28 | 2023-09-22 | 伊缪诺金公司 | 具有自分解肽接头的类美登素衍生物和其缀合物 |
TW201839001A (zh) | 2017-04-20 | 2018-11-01 | 美商伊繆諾金公司 | 細胞毒性苯并二氮平衍生物及其綴合物 |
US11471595B2 (en) | 2017-05-04 | 2022-10-18 | Milestone Scientific, Inc. | Method and apparatus for performing a peripheral nerve block |
WO2019133652A1 (en) | 2017-12-28 | 2019-07-04 | Immunogen, Inc. | Benzodiazepine derivatives |
CN113631194A (zh) | 2019-03-21 | 2021-11-09 | 伊缪诺金公司 | 制备细胞结合剂-药物缀合物的方法 |
JP2022529583A (ja) | 2019-03-29 | 2022-06-23 | イミュノジェン・インコーポレーテッド | 異常細胞増殖を阻害するまたは増殖性疾患を治療するための細胞毒性ビス-ベンゾジアゼピン誘導体及び細胞結合剤とのその複合体 |
FI3958977T3 (fi) | 2019-04-26 | 2023-12-12 | Immunogen Inc | Kamptotesiinijohdannaisia |
US10646660B1 (en) | 2019-05-16 | 2020-05-12 | Milestone Scientific, Inc. | Device and method for identification of a target region |
MX2022007122A (es) | 2019-12-11 | 2022-09-09 | Molecular Partners Ag | Dominios de repetición de anquirina diseñados con residuos de superficie alterados. |
CA3176845A1 (en) | 2020-05-06 | 2021-11-11 | Patrick AMSTUTZ | Novel ankyrin repeat binding proteins and their uses |
WO2022190016A1 (en) | 2021-03-09 | 2022-09-15 | Molecular Partners Ag | Novel darpin based multi-specific t-cell engagers |
Family Cites Families (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1078051B1 (en) | 1998-05-13 | 2007-12-12 | Domantis Limited | Phage-display system for the selection of correctly folded proteins |
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2006
- 2006-06-30 CA CA2614512A patent/CA2614512C/en not_active Expired - Fee Related
- 2006-06-30 DE DE602006010386T patent/DE602006010386D1/de active Active
- 2006-06-30 DK DK06763985.6T patent/DK1902131T3/da active
- 2006-06-30 CN CN2006800243863A patent/CN101213299B/zh not_active Expired - Fee Related
- 2006-06-30 EP EP06763985A patent/EP1902131B1/en active Active
- 2006-06-30 JP JP2008519913A patent/JP5042218B2/ja not_active Expired - Fee Related
- 2006-06-30 AT AT06763985T patent/ATE448305T1/de active
- 2006-06-30 WO PCT/EP2006/063729 patent/WO2007006665A1/en active Application Filing
- 2006-06-30 NZ NZ564695A patent/NZ564695A/en not_active IP Right Cessation
- 2006-06-30 US US11/988,389 patent/US8846577B2/en active Active
- 2006-06-30 ES ES06763985T patent/ES2335136T3/es active Active
- 2006-06-30 RU RU2008104174/10A patent/RU2412249C2/ru not_active IP Right Cessation
- 2006-06-30 BR BRPI0613593-5A patent/BRPI0613593A2/pt not_active Application Discontinuation
- 2006-06-30 MX MX2008000345A patent/MX2008000345A/es active IP Right Grant
- 2006-06-30 PL PL06763985T patent/PL1902131T3/pl unknown
- 2006-06-30 AU AU2006268780A patent/AU2006268780B2/en not_active Ceased
-
2007
- 2007-12-07 ZA ZA200710687A patent/ZA200710687B/xx unknown
- 2007-12-31 IL IL188518A patent/IL188518A/en not_active IP Right Cessation
-
2008
- 2008-02-05 NO NO20080665A patent/NO20080665L/no not_active Application Discontinuation
- 2008-02-05 KR KR1020087003094A patent/KR101268939B1/ko not_active IP Right Cessation
- 2008-12-23 HK HK08113901.3A patent/HK1122839A1/xx not_active IP Right Cessation
-
2014
- 2014-08-26 US US14/468,597 patent/US9447142B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
WO2007006665A1 (en) | 2007-01-18 |
ATE448305T1 (de) | 2009-11-15 |
KR101268939B1 (ko) | 2013-05-30 |
RU2008104174A (ru) | 2009-08-20 |
US9447142B2 (en) | 2016-09-20 |
JP5042218B2 (ja) | 2012-10-03 |
US20110118146A1 (en) | 2011-05-19 |
AU2006268780B2 (en) | 2011-03-24 |
MX2008000345A (es) | 2008-04-02 |
DE602006010386D1 (en) | 2009-12-24 |
PL1902131T3 (pl) | 2010-05-31 |
RU2412249C2 (ru) | 2011-02-20 |
DK1902131T3 (da) | 2010-03-01 |
ES2335136T3 (es) | 2010-03-22 |
KR20080035603A (ko) | 2008-04-23 |
EP1902131A1 (en) | 2008-03-26 |
ZA200710687B (en) | 2008-11-26 |
US8846577B2 (en) | 2014-09-30 |
EP1902131B1 (en) | 2009-11-11 |
AU2006268780A1 (en) | 2007-01-18 |
CN101213299A (zh) | 2008-07-02 |
IL188518A0 (en) | 2008-04-13 |
JP2009500027A (ja) | 2009-01-08 |
HK1122839A1 (en) | 2009-05-29 |
BRPI0613593A2 (pt) | 2011-01-18 |
IL188518A (en) | 2011-04-28 |
NZ564695A (en) | 2009-02-28 |
CA2614512C (en) | 2014-01-07 |
CA2614512A1 (en) | 2007-01-18 |
US20150057186A1 (en) | 2015-02-26 |
CN101213299B (zh) | 2013-03-27 |
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