EP2370470A4 - Screening von abundant sezernierten proteinen und deren verwendung als fusionspartner zur herstellung rekombinanter proteine - Google Patents

Screening von abundant sezernierten proteinen und deren verwendung als fusionspartner zur herstellung rekombinanter proteine

Info

Publication number
EP2370470A4
EP2370470A4 EP08878601A EP08878601A EP2370470A4 EP 2370470 A4 EP2370470 A4 EP 2370470A4 EP 08878601 A EP08878601 A EP 08878601A EP 08878601 A EP08878601 A EP 08878601A EP 2370470 A4 EP2370470 A4 EP 2370470A4
Authority
EP
European Patent Office
Prior art keywords
proteins
screening
production
fusion partners
abundantly secreted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP08878601A
Other languages
English (en)
French (fr)
Other versions
EP2370470B1 (de
EP2370470A1 (de
Inventor
Jung-Hoon Sohn
Jung-Hoon Bae
Hyun-Jin Kim
Kwang-Mook Lim
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Korea Research Institute of Bioscience and Biotechnology KRIBB
Original Assignee
Korea Research Institute of Bioscience and Biotechnology KRIBB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Korea Research Institute of Bioscience and Biotechnology KRIBB filed Critical Korea Research Institute of Bioscience and Biotechnology KRIBB
Priority to EP12187155.2A priority Critical patent/EP2573122A3/de
Priority to EP15160709.0A priority patent/EP2918606B1/de
Publication of EP2370470A1 publication Critical patent/EP2370470A1/de
Publication of EP2370470A4 publication Critical patent/EP2370470A4/de
Application granted granted Critical
Publication of EP2370470B1 publication Critical patent/EP2370470B1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1051—Gene trapping, e.g. exon-, intron-, IRES-, signal sequence-trap cloning, trap vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP08878601.7A 2008-12-04 2008-12-05 Screening von abundant sezernierten proteinen und deren verwendung als fusionspartner zur herstellung rekombinanter proteine Active EP2370470B1 (de)

Priority Applications (2)

Application Number Priority Date Filing Date Title
EP12187155.2A EP2573122A3 (de) 2008-12-04 2008-12-05 Screening von abundant sezernierten Proteinen und deren Verwendung als Fusionspartner zur Herstellung rekombinanter Proteine
EP15160709.0A EP2918606B1 (de) 2008-12-04 2008-12-05 Screening von abundant sezernierten Proteinen und deren Verwendung als Fusionspartner zur Herstellung rekombinanter Proteine

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11997208P 2008-12-04 2008-12-04
PCT/KR2008/007231 WO2010064748A1 (en) 2008-12-04 2008-12-05 Screening of abundantly secreted proteins and their use as fusion partners for the production of recombinant proteins

Related Child Applications (3)

Application Number Title Priority Date Filing Date
EP15160709.0A Division EP2918606B1 (de) 2008-12-04 2008-12-05 Screening von abundant sezernierten Proteinen und deren Verwendung als Fusionspartner zur Herstellung rekombinanter Proteine
EP15160709.0A Division-Into EP2918606B1 (de) 2008-12-04 2008-12-05 Screening von abundant sezernierten Proteinen und deren Verwendung als Fusionspartner zur Herstellung rekombinanter Proteine
EP12187155.2A Division-Into EP2573122A3 (de) 2008-12-04 2008-12-05 Screening von abundant sezernierten Proteinen und deren Verwendung als Fusionspartner zur Herstellung rekombinanter Proteine

Publications (3)

Publication Number Publication Date
EP2370470A1 EP2370470A1 (de) 2011-10-05
EP2370470A4 true EP2370470A4 (de) 2012-04-11
EP2370470B1 EP2370470B1 (de) 2016-10-05

Family

ID=42233390

Family Applications (3)

Application Number Title Priority Date Filing Date
EP15160709.0A Active EP2918606B1 (de) 2008-12-04 2008-12-05 Screening von abundant sezernierten Proteinen und deren Verwendung als Fusionspartner zur Herstellung rekombinanter Proteine
EP08878601.7A Active EP2370470B1 (de) 2008-12-04 2008-12-05 Screening von abundant sezernierten proteinen und deren verwendung als fusionspartner zur herstellung rekombinanter proteine
EP12187155.2A Withdrawn EP2573122A3 (de) 2008-12-04 2008-12-05 Screening von abundant sezernierten Proteinen und deren Verwendung als Fusionspartner zur Herstellung rekombinanter Proteine

Family Applications Before (1)

Application Number Title Priority Date Filing Date
EP15160709.0A Active EP2918606B1 (de) 2008-12-04 2008-12-05 Screening von abundant sezernierten Proteinen und deren Verwendung als Fusionspartner zur Herstellung rekombinanter Proteine

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP12187155.2A Withdrawn EP2573122A3 (de) 2008-12-04 2008-12-05 Screening von abundant sezernierten Proteinen und deren Verwendung als Fusionspartner zur Herstellung rekombinanter Proteine

Country Status (6)

Country Link
US (1) US9550997B2 (de)
EP (3) EP2918606B1 (de)
JP (2) JP5524977B2 (de)
KR (3) KR101498668B1 (de)
CN (2) CN103952425B (de)
WO (1) WO2010064748A1 (de)

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DK2275556T3 (da) 2004-01-17 2013-07-08 Korea Res Inst Of Bioscience Hurtig fremgangsmåde til screening af translationsfusionspartnere til fremstilling af rekombinante proteiner og translationsfusionpartnere screenet derfra
WO2012060666A2 (ko) * 2010-11-04 2012-05-10 한국생명공학연구원 효모에서 인체 상피세포 성장인자를 대량 생산하는 방법
KR20130132789A (ko) * 2010-11-05 2013-12-05 아사히 가라스 가부시키가이샤 스키조사카로미세스속 효모의 형질 전환체 및 그 제조 방법
CN102206615B (zh) * 2011-04-27 2013-06-12 珠海市御品堂生物科技有限公司 一种用酶解法制备乙醇脱氢酶的工艺
CN102732549B (zh) * 2012-06-20 2014-04-23 上海普欣生物技术有限公司 一种重组胰岛素样生长因子-i的制备方法
EP3027752B1 (de) * 2013-07-01 2018-08-08 Biocon Limited Signalsequenz für proteinexpression in pichia pastoris
EP2871235A1 (de) * 2013-11-07 2015-05-13 Centre National de la Recherche Scientifique (CNRS) Neues Verfahren zur Herstellung von aktiver hTERT
CN103709253A (zh) * 2013-12-20 2014-04-09 南开大学 一种生物合成制备glp-1及其类似物的方法
WO2015141705A1 (ja) * 2014-03-19 2015-09-24 独立行政法人産業技術総合研究所 耐酸耐塩性付与方法と耐酸耐塩性酵母を用いた有用物質生産
CN104232666A (zh) * 2014-09-01 2014-12-24 江苏海王生物制药有限公司 表达重组艾塞那肽的基因及其载体
KR102353262B1 (ko) * 2014-12-01 2022-01-18 피페넥스 인크. 펩티드 생산용 융합 파트너
CN105219663B (zh) * 2015-09-18 2018-10-23 上海交通大学 合成海藻糖的专用菌株及其用于合成海藻糖的方法
WO2017120890A1 (zh) * 2016-01-15 2017-07-20 江南大学 一种利用磷脂酶融合表达以提高外源蛋白表达量的方法
CN108192910A (zh) * 2017-09-19 2018-06-22 陈玉皎 培育降血糖和降血栓的转基因植物
KR20200120908A (ko) * 2018-02-12 2020-10-22 론자 리미티드 목적 단백질을 생산하기 위한 숙주 세포
WO2019178056A1 (en) * 2018-03-12 2019-09-19 Massachusetts Institute Of Technology Computational platform for in silico combinatorial sequence space exploration and artificial evolution of peptides
CN108707634B (zh) * 2018-06-05 2021-08-24 江南大学 一种多酶偶联生产海藻糖的方法及其应用
CN114032307A (zh) * 2018-08-28 2022-02-11 博尔诚（北京）科技有限公司 用于检测食道癌的组合物及其用途
CN110373340A (zh) * 2019-07-12 2019-10-25 广东利世康低碳科技有限公司 一种降解餐厨废弃物的产朊假丝酵母单基因表达菌株及其构建方法
CN110498859B (zh) * 2019-07-29 2021-07-02 因之彩生物科技（武汉）有限公司 重组PLB-hbFGF融合蛋白及其应用
CN110734490A (zh) * 2019-11-28 2020-01-31 苏州华研医用科技有限公司 重组人金属硫蛋白ⅲ纯品的制备方法
KR102613936B1 (ko) * 2020-11-13 2023-12-15 한국생명공학연구원 자가분해능이 저하된 켁신 효소 및 이의 생산 방법
CN113087787B (zh) * 2021-05-19 2023-03-21 广西医科大学第二附属医院(广西医科大学第二临床医学院) 一种蚯蚓血红蛋白分离及纯化方法
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EP2918606A2 (de) 2015-09-16
EP2370470B1 (de) 2016-10-05
KR20130091798A (ko) 2013-08-19
JP2012510808A (ja) 2012-05-17
KR20110104512A (ko) 2011-09-22
JP5955875B2 (ja) 2016-07-20
EP2370470A1 (de) 2011-10-05
EP2573122A3 (de) 2013-07-10
JP2014138600A (ja) 2014-07-31
CN102239183A (zh) 2011-11-09
KR20130091797A (ko) 2013-08-19
JP5524977B2 (ja) 2014-06-18
EP2573122A2 (de) 2013-03-27
EP2918606A3 (de) 2015-10-28
CN102239183B (zh) 2017-06-30
KR101387693B1 (ko) 2014-04-21
CN103952425B (zh) 2017-04-12
KR101431091B1 (ko) 2014-08-26
US9550997B2 (en) 2017-01-24
WO2010064748A1 (en) 2010-06-10
KR101498668B1 (ko) 2015-03-06
EP2918606B1 (de) 2018-07-11
US20100159465A1 (en) 2010-06-24
CN103952425A (zh) 2014-07-30

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