EP1997912A3 - Vorrichtung und Verfahren zur Quantifizierung von MRNA mit hohem Durchlauf aus Vollblut - Google Patents

Vorrichtung und Verfahren zur Quantifizierung von MRNA mit hohem Durchlauf aus Vollblut Download PDF

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Publication number
EP1997912A3
EP1997912A3 EP08014198A EP08014198A EP1997912A3 EP 1997912 A3 EP1997912 A3 EP 1997912A3 EP 08014198 A EP08014198 A EP 08014198A EP 08014198 A EP08014198 A EP 08014198A EP 1997912 A3 EP1997912 A3 EP 1997912A3
Authority
EP
European Patent Office
Prior art keywords
mrna
whole blood
throughput quantification
kit
automated system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP08014198A
Other languages
English (en)
French (fr)
Other versions
EP1997912A2 (de
EP1997912B1 (de
Inventor
Masato Mitsuhashi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Resonac Corp
Resonac America Inc
Original Assignee
Hitachi Chemical Co Ltd
Hitachi Chemical Research Center Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hitachi Chemical Co Ltd, Hitachi Chemical Research Center Inc filed Critical Hitachi Chemical Co Ltd
Publication of EP1997912A2 publication Critical patent/EP1997912A2/de
Publication of EP1997912A3 publication Critical patent/EP1997912A3/de
Application granted granted Critical
Publication of EP1997912B1 publication Critical patent/EP1997912B1/de
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C12N1/066—Lysis of microorganisms by physical methods
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6846—Common amplification features

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
EP08014198A 2003-10-30 2004-10-29 Vorrichtung und Verfahren zur Quantifizierung von MRNA mit hohem Durchlauf aus Vollblut Not-in-force EP1997912B1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US69896703A 2003-10-30 2003-10-30
US10/796,298 US7745180B2 (en) 2002-04-24 2004-03-09 Device and method for high-throughput quantification of mRNA from whole blood
EP04796890A EP1682678B1 (de) 2003-10-30 2004-10-29 Vorrichtung und verfahren zur quantifizierung von mrna aus vollblut mit hohem durchsatz

Related Parent Applications (2)

Application Number Title Priority Date Filing Date
EP04796890A Division EP1682678B1 (de) 2003-10-30 2004-10-29 Vorrichtung und verfahren zur quantifizierung von mrna aus vollblut mit hohem durchsatz
EP04796890.4 Division 2004-10-29

Publications (3)

Publication Number Publication Date
EP1997912A2 EP1997912A2 (de) 2008-12-03
EP1997912A3 true EP1997912A3 (de) 2008-12-10
EP1997912B1 EP1997912B1 (de) 2010-04-28

Family

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Family Applications (4)

Application Number Title Priority Date Filing Date
EP08014198A Not-in-force EP1997912B1 (de) 2003-10-30 2004-10-29 Vorrichtung und Verfahren zur Quantifizierung von MRNA mit hohem Durchlauf aus Vollblut
EP04796890A Not-in-force EP1682678B1 (de) 2003-10-30 2004-10-29 Vorrichtung und verfahren zur quantifizierung von mrna aus vollblut mit hohem durchsatz
EP08075176A Not-in-force EP1953245B1 (de) 2003-10-30 2004-10-29 Vorrichtung und Verfahren zur Quantifizierung von MRNA mit hohem Durchlauf aus Vollblut
EP08014199A Not-in-force EP2058405B1 (de) 2003-10-30 2004-10-29 Vorrichtung und Verfahren zur Quantifizierung von mRNA mit hohem Durchlauf aus Vollblut

Family Applications After (3)

Application Number Title Priority Date Filing Date
EP04796890A Not-in-force EP1682678B1 (de) 2003-10-30 2004-10-29 Vorrichtung und verfahren zur quantifizierung von mrna aus vollblut mit hohem durchsatz
EP08075176A Not-in-force EP1953245B1 (de) 2003-10-30 2004-10-29 Vorrichtung und Verfahren zur Quantifizierung von MRNA mit hohem Durchlauf aus Vollblut
EP08014199A Not-in-force EP2058405B1 (de) 2003-10-30 2004-10-29 Vorrichtung und Verfahren zur Quantifizierung von mRNA mit hohem Durchlauf aus Vollblut

Country Status (7)

Country Link
US (6) US7745180B2 (de)
EP (4) EP1997912B1 (de)
JP (3) JP4594321B2 (de)
CN (4) CN101696448A (de)
AT (4) ATE442458T1 (de)
DE (3) DE602004023143D1 (de)
WO (1) WO2005042784A2 (de)

Families Citing this family (68)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6844158B1 (en) * 1997-12-22 2005-01-18 Hitachi Chemical Co., Ltd. Direct RT-PCR on oligonucleotide-immobilized PCR microplates
SE9904539D0 (sv) * 1999-12-10 1999-12-10 Alphahelix Ab Method and device for the handling of samples and reagents
US6638428B2 (en) * 2000-10-31 2003-10-28 Hitachi Chemical Research Center, Inc. Method of preventing formation of bubbles during filtration operations
EP1330518B1 (de) * 2000-10-31 2010-09-08 Hitachi Chemical Research Center, Inc. Methode für das sammeln und das verwenden von kern-mrna
US7745180B2 (en) * 2002-04-24 2010-06-29 Hitachi Chemical Co., Ltd. Device and method for high-throughput quantification of mRNA from whole blood
EP1776471B9 (de) * 2004-05-25 2013-12-18 Hitachi Chemical Company, Ltd. Verfahren zur messung der anfälligkeit für krebs
US20050277121A1 (en) 2004-06-11 2005-12-15 Ambion, Inc. Crude biological derivatives competent for nucleic acid detection
JP2006083114A (ja) * 2004-09-17 2006-03-30 Hitachi High-Technologies Corp 核酸抽出方法、及び核酸遊離方法
US20090011410A1 (en) * 2004-10-20 2009-01-08 Masato Mitsuhashi Method for tailoring administration of drugs by quantitation of mrna
JP2008526255A (ja) * 2005-01-13 2008-07-24 マイクロニクス， インコーポレイテッド 微量流体希薄細胞検出デバイス
ATE512234T1 (de) * 2005-04-28 2011-06-15 Hitachi Chemical Res Ct Inc Ex-vivo-genexpression in vollblut als modell für die beurteilung von individueller variation gegenüber nahrungszusätzen
ATE493515T1 (de) 2005-06-08 2011-01-15 Hitachi Chemical Res Ct Inc Verfahren zur vorhersage der immunreaktion auf neoplastische krankheiten auf der grundlage des mrna-ausdrucksprofil in neoplastischen zellen und stimulierten leukozyten
US7569367B2 (en) * 2005-09-07 2009-08-04 Roche Diagnostics Operations, Inc. Nucleic acid preparation from whole blood for use in diagnosis of transmissible spongiform encephalopathy
US8979770B2 (en) * 2006-02-24 2015-03-17 Merck Sharp & Dohme Corp. Extraction and diagnostic fluid devices, systems and methods of use
US20080075789A1 (en) * 2006-02-28 2008-03-27 The Regents Of The University Of California Genes differentially expressed in bipolar disorder and/or schizophrenia
WO2009070442A2 (en) * 2007-11-14 2009-06-04 Hitachi Chemical Co. Ltd. Fc receptor-mediated tumor necrosis factor superfamily mrna expression in peripheral blood leukocytes
US20090311684A1 (en) * 2006-04-07 2009-12-17 Hitachi Chemical Co., Ltd Enhanced fc receptor-mediated tumor necrosis factor superfamily and chemokine mrna expression in peripheral blood leukocytes in patients with rheumatoid arthritis
US7838239B2 (en) * 2006-04-07 2010-11-23 Hitachi Chemical Co., Ltd. Methods regarding enhanced T-cell receptor-mediated tumor necrosis factor superfamily mRNA expression in peripheral blood leukocytes in patients with crohn's disease
CN101437549A (zh) * 2006-05-08 2009-05-20 日立化成工业株式会社 通过对薄切肿瘤组织中mRNA表达进行定量测试实体瘤中药物敏感性的方法
EP2027293B1 (de) * 2006-05-24 2014-11-19 Gen-Probe Incorporated Verfahren zum lysieren von mycobacterium und amplifikation seiner nukleinsäuren
US20080153078A1 (en) * 2006-06-15 2008-06-26 Braman Jeffrey C System for isolating biomolecules from a sample
US8097419B2 (en) 2006-09-12 2012-01-17 Longhorn Vaccines & Diagnostics Llc Compositions and method for rapid, real-time detection of influenza A virus (H1N1) swine 2009
US9481912B2 (en) 2006-09-12 2016-11-01 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and identifying nucleic acid sequences in biological samples
US8080645B2 (en) 2007-10-01 2011-12-20 Longhorn Vaccines & Diagnostics Llc Biological specimen collection/transport compositions and methods
WO2008116150A2 (en) 2007-03-21 2008-09-25 Cedars-Sinai Medical Center Ileal pouch-anal anastomosis (ipaa) factors in the treatment of inflammatory bowel disease
US9683256B2 (en) 2007-10-01 2017-06-20 Longhorn Vaccines And Diagnostics, Llc Biological specimen collection and transport system
US11041215B2 (en) 2007-08-24 2021-06-22 Longhorn Vaccines And Diagnostics, Llc PCR ready compositions and methods for detecting and identifying nucleic acid sequences
US10004799B2 (en) 2007-08-27 2018-06-26 Longhorn Vaccines And Diagnostics, Llc Composite antigenic sequences and vaccines
AU2008293504B2 (en) 2007-08-27 2012-04-12 Longhorn Vaccines & Diagnostics, Llc Immunogenic compositions and methods
AU2008343745B2 (en) 2007-10-01 2012-05-10 Longhorn Vaccines & Diagnostics Llc Biological specimen collection and transport system and methods of use
US11041216B2 (en) 2007-10-01 2021-06-22 Longhorn Vaccines And Diagnostics, Llc Compositions and methods for detecting and quantifying nucleic acid sequences in blood samples
US20090215064A1 (en) * 2008-02-27 2009-08-27 Hitachi Chemical Co., Ltd. Quantitative assessment of individual cancer susceptibility by measuring dna damage-induced mrna in whole blood
US8357672B2 (en) * 2008-06-13 2013-01-22 Bio-Rad Laboratories, Inc. Cell lysis reagent for isolation of RNA
WO2010062960A2 (en) 2008-11-26 2010-06-03 Cedars-Sinai Medical Center METHODS OF DETERMINING RESPONSIVENESS TO ANTI-TNFα THERAPY IN INFLAMMATORY BOWEL DISEASE
WO2010075579A2 (en) 2008-12-24 2010-07-01 Cedars-Sinai Medical Center Methods of predicting medically refractive ulcerative colitis (mr-uc) requiring colectomy
JPWO2011004517A1 (ja) * 2009-07-06 2012-12-13 オリンパス株式会社 標的核酸の検出方法、及び大腸癌の検査方法
WO2011156763A1 (en) 2010-06-11 2011-12-15 Hitachi Chemical Co., Ltd. Methods for characterizing kidney function
WO2011156734A2 (en) 2010-06-11 2011-12-15 Hitachi Chemical Co., Ltd. Method of characterizing vascular diseases
US8721413B2 (en) * 2010-10-06 2014-05-13 Jameel Akhtab Mohammed Computer game with a target number of formations created from falling pieces in a triangular grid
WO2012170037A1 (en) 2011-06-10 2012-12-13 Hitachi Chemical Co., Ltd. Vesicle capturing devices and methods for using same
JP5837691B2 (ja) 2011-07-18 2015-12-24 日立化成株式会社 白血球機能関連ｍＲＮＡの生体外での誘導によって癌免疫療法に対するホストの応答性を予測する方法
ES2665252T3 (es) 2011-12-30 2018-04-25 Abbott Molecular Inc. Purificación de ácido nucleico de microorganismos a partir de muestras del hospedador
US20130172208A1 (en) * 2012-01-04 2013-07-04 Hitachi Chemical Research Center, Inc. ASSESSMENT OF BONE MARROW RECOVERY BY MEASURING PLASMA EXOSOME mRNAS
EP2806890A4 (de) 2012-01-26 2015-09-02 Longhorn Vaccines & Diagnostics Llc Zusammengesetzte antigensequenzen und impfstoffe
EP3639841B1 (de) 2013-03-27 2023-09-06 Cedars-Sinai Medical Center Behandlung von fibrose durch tl1a-hemmung
JP6297676B2 (ja) 2013-05-06 2018-03-20 日立化成株式会社 標的分子を捕捉するためのデバイス及び方法
US10316083B2 (en) 2013-07-19 2019-06-11 Cedars-Sinai Medical Center Signature of TL1A (TNFSF15) signaling pathway
CN103645332B (zh) * 2013-12-19 2015-04-29 南京卡迪奥密生物技术有限公司 自动化高通量检测胆固醇以及其它分析物细胞流出的多功能工作站
JP6113674B2 (ja) * 2014-02-13 2017-04-12 株式会社神戸製鋼所 耐熱性に優れる表面被覆層付き銅合金板条
USD750785S1 (en) * 2014-04-04 2016-03-01 Hitachi Chemical Company, Ltd. Filter sheet for a cell collecting cartridge
USD747487S1 (en) * 2014-04-04 2016-01-12 Hitachi Chemical Company, Ltd. Filter sheet for a cell collecting cartridge
USD746467S1 (en) * 2014-04-04 2015-12-29 Hitachi Chemical Company, Ltd. Filter sheet for a cell collecting cartridge
USD748267S1 (en) * 2014-04-04 2016-01-26 Hitachi Chemical Company, Ltd. Filter sheet for a cell collecting cartridge
USD748801S1 (en) * 2014-04-04 2016-02-02 Hitachi Chemical Company, Ltd. Filter sheet for a cell collecting cartridge
US10266895B2 (en) * 2014-11-05 2019-04-23 Hitachi Chemical Company Ltd. Exosomes and microvesicles in intestinal luminal fluids and stool and use of same for the assessment of inflammatory bowel disease
US10370719B2 (en) 2014-11-12 2019-08-06 Hitachi Chemical Co., Ltd. Method and device for diagnosing organ injury
EP3288459B1 (de) * 2015-04-29 2023-09-20 Revvity Health Sciences, Inc. Probenahme- und ausgabevorrichtung
CA2985652C (en) 2015-05-14 2020-03-10 Gerald W. FISHER Rapid methods for the extraction of nucleic acids from biological samples
US11028443B2 (en) 2015-08-31 2021-06-08 Showa Denko Materials Co., Ltd. Molecular methods for assessing urothelial disease
US11186872B2 (en) 2016-03-17 2021-11-30 Cedars-Sinai Medical Center Methods of diagnosing inflammatory bowel disease through RNASET2
CN106190817B (zh) * 2016-07-19 2019-01-08 温州市高亿诺生物科技有限公司 基于多孔板的核酸裂解液分离方法和装置
KR20180099964A (ko) * 2017-02-27 2018-09-06 (주)오상헬스케어 혈액 분석용 스트립
US20190283022A1 (en) * 2018-03-15 2019-09-19 Behrad Vahidi Highly parallel microfluidic blood separation device
MX2021009169A (es) * 2019-02-11 2021-10-13 Ethris Gmbh Purificacion de arnm por filtracion de flujo tangencial.
KR20230054790A (ko) * 2019-07-16 2023-04-25 멜리오랩스 인코포레이티드 단일-세포 기반 디지털 고해상도 용융을 위한 방법 및 장치
CN110452998A (zh) * 2019-09-17 2019-11-15 浙江海洋大学 利用线粒体dna控制区进行黄鲫遗传学分析的方法
CN110724633A (zh) * 2019-11-11 2020-01-24 浙江汇泽医药科技有限公司 一种微量细胞核酸提取与扩增系统及工艺
KR102375663B1 (ko) * 2020-03-24 2022-03-16 재단법인대구경북과학기술원 무손실 세포 염색 방법 및 장치

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1138780A1 (de) * 2000-03-31 2001-10-04 Boehringer Mannheim Gmbh Detektion ausgesäter Tumorzellen mittels quantitativer RT-PCR auf Cytokeratin-20 mRNA

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4193980A (en) 1978-01-05 1980-03-18 Corning Glass Works Dry preparation for reticulocyte staining
US4652525A (en) * 1978-04-19 1987-03-24 The Regents Of The University Of California Recombinant bacterial plasmids containing the coding sequences of insulin genes
US5175109A (en) 1986-09-10 1992-12-29 Toa Medical Electronics Co., Ltd. Reagent for classifying leukocytes by flow cytometry
US4925572A (en) 1987-10-20 1990-05-15 Pall Corporation Device and method for depletion of the leukocyte content of blood and blood components
US4923620A (en) * 1987-10-20 1990-05-08 Pall Corporation Device for depletion of the leukocyte content of blood and blood components
US4880548A (en) 1988-02-17 1989-11-14 Pall Corporation Device and method for separating leucocytes from platelet concentrate
US4958013A (en) * 1989-06-06 1990-09-18 Northwestern University Cholesteryl modified oligonucleotides
US5045449A (en) * 1989-06-14 1991-09-03 Thomas Jefferson University Methods of detecting a genetic predisposition for vascular aneurysms
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
GB9027055D0 (en) * 1990-12-13 1991-02-06 Sandoz Ltd Organic compounds
US6300058B1 (en) 1992-01-29 2001-10-09 Hitachi Chemical Research Center, Inc. Method for measuring messenger RNA
IL104670A (en) 1993-02-09 1998-04-05 Travenol Lab Israel Ltd Leukocyte removal method and filter unit for same
GB9314249D0 (en) 1993-07-09 1993-08-18 Proofname Ltd Purification method and apparatus
NO180658C (no) 1994-03-10 1997-05-21 Oeystein Fodstad Fremgangsmåte og anordning for deteksjon av spesifikke målceller i spesialiserte eller blandede cellepopulasjoner og opplösninger som inneholder blandede cellepopulasjoner
US5491063A (en) 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
US5691132A (en) * 1994-11-14 1997-11-25 Cerus Corporation Method for inactivating pathogens in red cell compositions using quinacrine mustard
US5801064A (en) * 1995-12-04 1998-09-01 Foresman; Mark D. Assay methods and reagents for detecting autoantibodies
US6042859A (en) 1996-04-18 2000-03-28 Ramot University Authority For Applied Research And Industrial Development Ltd. Method for the long-term preservation of meat
JP3249919B2 (ja) 1996-07-30 2002-01-28 株式会社堀場製作所 免疫測定方法
US5976797A (en) 1996-12-20 1999-11-02 Hitachi Chemical Co., Ltd. Method for quantifying total mRNA with poly(A)-complementary oligonucleotide-immobilized microtiter plate
US5948666A (en) * 1997-08-06 1999-09-07 Diversa Corporation Isolation and identification of polymerases
AU9186598A (en) 1997-09-26 1999-04-23 Kyowa Hakko Kogyo Co. Ltd. Killer t cell receptor recognizing human immunodeficiency virus
DE69839572D1 (de) * 1997-12-22 2008-07-10 Hitachi Chemical Co Ltd Reinigung von mRNA auf Mikroplatten
US6844158B1 (en) 1997-12-22 2005-01-18 Hitachi Chemical Co., Ltd. Direct RT-PCR on oligonucleotide-immobilized PCR microplates
ATE242780T1 (de) 1998-10-09 2003-06-15 Whatman Bioscience Ltd Verfahren zur isolierung von nukleinsäure und vorrichtung hierfür
DE19914966A1 (de) * 1999-04-01 2000-10-05 Basf Ag Verfahren zur kontinuierlich betriebenen destillativen Abtrennung eines höherschmelzenden Stoffes
WO2000077253A1 (fr) 1999-06-16 2000-12-21 Hitachi, Ltd. Appareil et procede d'examen des genes
JP3707303B2 (ja) 1999-06-30 2005-10-19 株式会社日立製作所 ヒスタミン計測方法およびヒスタミン計測装置
ES2264440T3 (es) * 2000-03-24 2007-01-01 Micromet Ag Amplificacion de mrna.
US20030170669A1 (en) * 2000-04-11 2003-09-11 Garvin Alex M. Method of nucleic acid recovery
EP1337670A4 (de) 2000-08-16 2004-07-14 Whatman Inc Transfusionsmedizinfiltriermittel zur abreicherung von leukozyten als quelle für genetisches material für genotypisierungsstudien
WO2002021133A2 (en) * 2000-09-07 2002-03-14 The Brigham And Women's Hospital, Inc. Methods of detecting cancer based on prostasin
US6602718B1 (en) * 2000-11-08 2003-08-05 Becton, Dickinson And Company Method and device for collecting and stabilizing a biological sample
BR0115715A (pt) * 2000-11-28 2004-02-03 Wyeth Corp Análise de expressão de ácidos nucleìcos e polipeptìdeos úteis na diagnose e tratamento de câncer da próstata
US6638722B2 (en) 2001-06-13 2003-10-28 Invitrogen Corporation Method for rapid amplification of DNA
WO2003062462A2 (en) 2002-01-16 2003-07-31 Dynal Biotech Asa Method for isolating nucleic acids and protein from a single sample
US7745180B2 (en) 2002-04-24 2010-06-29 Hitachi Chemical Co., Ltd. Device and method for high-throughput quantification of mRNA from whole blood
CN1188529C (zh) * 2002-06-11 2005-02-09 刘全俊 一种基因芯片的纳米金标记银染检测法
US20050059024A1 (en) * 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1138780A1 (de) * 2000-03-31 2001-10-04 Boehringer Mannheim Gmbh Detektion ausgesäter Tumorzellen mittels quantitativer RT-PCR auf Cytokeratin-20 mRNA

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
HAMAGUCHI Y ET AL: "Direct reverse transcription-PCR on oligo(dT)-immobilized polypropylene microplates after capturing total mRNA from crude cell lysates", CLINICAL CHEMISTRY, AMERICAN ASSOCIATION FOR CLINICAL CHEMISTRY, WASHINGTON, DC, vol. 44, no. 11, 1 January 1998 (1998-01-01), pages 2256 - 2263, XP002208861, ISSN: 0009-9147 *
MITSUHASHI MASATO ET AL: "Quantification of mRNA in whole blood by assessing recovery of RNA and efficiency of cDNA synthesis.", CLINICAL CHEMISTRY APR 2006, vol. 52, no. 4, April 2006 (2006-04-01), pages 634 - 642, XP002500777, ISSN: 0009-9147 *
TAKASHI ISHIKAWA ET AL: "CONSTRUCTION OF CDNA BANK FROM BIOPSY SPECIMENS FOR MULTIPLE GENE ANALYSIS OF CANCER", CLINICAL CHEMISTRY, AMERICAN ASSOCIATION FOR CLINICAL CHEMISTRY. WINSTON, US, vol. 43, no. 5, 1997, pages 764 - 770, XP002917361, ISSN: 0009-9147 *
TOMINAGA K ET AL: "Colorimetric ELISA measurement of specific mRNA on immobilized-oligonucleotide-coated microtiter plates by reverse transcription with biotinylated mononucleotides.", CLINICAL CHEMISTRY NOV 1996, vol. 42, no. 11, November 1996 (1996-11-01), pages 1750 - 1757, XP002500776, ISSN: 0009-9147 *
VLEMS F A ET AL: "Reliability of quantitative reverse-transcriptase-PCR-based detection of tumour cells in the blood between different laboratories using a standardised protocol", EUROPEAN JOURNAL OF CANCER, PERGAMON PRESS, OXFORD, GB, vol. 39, no. 3, 1 February 2003 (2003-02-01), pages 388 - 396, XP004405028, ISSN: 0959-8049 *

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DE602004023143D1 (de) 2009-10-22
DE602004030472D1 (de) 2011-01-20
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US8101344B2 (en) 2012-01-24
WO2005042784A3 (en) 2005-10-13
JP5160591B2 (ja) 2013-03-13
US7968288B1 (en) 2011-06-28
EP2058405A1 (de) 2009-05-13
US20080009009A1 (en) 2008-01-10
JP4594321B2 (ja) 2010-12-08
EP1953245A1 (de) 2008-08-06
US7745180B2 (en) 2010-06-29
US20070224632A1 (en) 2007-09-27
US20040265864A1 (en) 2004-12-30
EP1997912A2 (de) 2008-12-03
WO2005042784A2 (en) 2005-05-12
CN101696442B (zh) 2013-04-17
JP2010220619A (ja) 2010-10-07
US7939300B2 (en) 2011-05-10
EP1682678A2 (de) 2006-07-26
ATE491042T1 (de) 2010-12-15
JP2010162050A (ja) 2010-07-29
CN1997752A (zh) 2007-07-11
CN101696409B (zh) 2012-06-27
EP2058405B1 (de) 2011-07-20
US20060199212A1 (en) 2006-09-07
CN101696442A (zh) 2010-04-21
US7981608B2 (en) 2011-07-19
DE602004026947D1 (de) 2010-06-10
EP1997912B1 (de) 2010-04-28
JP2007509633A (ja) 2007-04-19
CN101696448A (zh) 2010-04-21
ATE442458T1 (de) 2009-09-15
JP5160592B2 (ja) 2013-03-13
ATE466110T1 (de) 2010-05-15
US8076105B2 (en) 2011-12-13
ATE517193T1 (de) 2011-08-15
EP1682678B1 (de) 2010-12-08
US20070224631A1 (en) 2007-09-27

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