DE602004030472D1 - Vorrichtung und verfahren zur quantifizierung von mrna aus vollblut mit hohem durchsatz - Google Patents
Vorrichtung und verfahren zur quantifizierung von mrna aus vollblut mit hohem durchsatzInfo
- Publication number
- DE602004030472D1 DE602004030472D1 DE602004030472T DE602004030472T DE602004030472D1 DE 602004030472 D1 DE602004030472 D1 DE 602004030472D1 DE 602004030472 T DE602004030472 T DE 602004030472T DE 602004030472 T DE602004030472 T DE 602004030472T DE 602004030472 D1 DE602004030472 D1 DE 602004030472D1
- Authority
- DE
- Germany
- Prior art keywords
- high throughput
- full blood
- quantifying mrna
- mrna
- kit
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000000034 method Methods 0.000 title abstract 3
- 210000004369 blood Anatomy 0.000 title abstract 2
- 239000008280 blood Substances 0.000 title abstract 2
- 108020004999 messenger RNA Proteins 0.000 title abstract 2
- 210000000265 leukocyte Anatomy 0.000 abstract 1
- 238000011002 quantification Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
- C12N1/066—Lysis of microorganisms by physical methods
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6846—Common amplification features
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US69896703A | 2003-10-30 | 2003-10-30 | |
| US10/796,298 US7745180B2 (en) | 2002-04-24 | 2004-03-09 | Device and method for high-throughput quantification of mRNA from whole blood |
| PCT/US2004/036309 WO2005042784A2 (en) | 2003-10-30 | 2004-10-29 | Device and method for high-throughput quantification of mrna from whole blood |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE602004030472D1 true DE602004030472D1 (de) | 2011-01-20 |
Family
ID=34556635
Family Applications (3)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE602004026947T Active DE602004026947D1 (de) | 2003-10-30 | 2004-10-29 | Vorrichtung und Verfahren zur Quantifizierung von MRNA mit hohem Durchlauf aus Vollblut |
| DE602004023143T Active DE602004023143D1 (de) | 2003-10-30 | 2004-10-29 | Vorrichtung und Verfahren zur Quantifizierung von MRNA mit hohem Durchlauf aus Vollblut |
| DE602004030472T Active DE602004030472D1 (de) | 2003-10-30 | 2004-10-29 | Vorrichtung und verfahren zur quantifizierung von mrna aus vollblut mit hohem durchsatz |
Family Applications Before (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE602004026947T Active DE602004026947D1 (de) | 2003-10-30 | 2004-10-29 | Vorrichtung und Verfahren zur Quantifizierung von MRNA mit hohem Durchlauf aus Vollblut |
| DE602004023143T Active DE602004023143D1 (de) | 2003-10-30 | 2004-10-29 | Vorrichtung und Verfahren zur Quantifizierung von MRNA mit hohem Durchlauf aus Vollblut |
Country Status (7)
| Country | Link |
|---|---|
| US (6) | US7745180B2 (de) |
| EP (4) | EP1997912B1 (de) |
| JP (3) | JP4594321B2 (de) |
| CN (4) | CN101696442B (de) |
| AT (4) | ATE491042T1 (de) |
| DE (3) | DE602004026947D1 (de) |
| WO (1) | WO2005042784A2 (de) |
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| US20080261207A1 (en) * | 2004-05-25 | 2008-10-23 | Masato Mitsuhashi | Method of Measuring Cancer Susceptibility |
| US20050277121A1 (en) | 2004-06-11 | 2005-12-15 | Ambion, Inc. | Crude biological derivatives competent for nucleic acid detection |
| JP2006083114A (ja) * | 2004-09-17 | 2006-03-30 | Hitachi High-Technologies Corp | 核酸抽出方法、及び核酸遊離方法 |
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| US6638722B2 (en) | 2001-06-13 | 2003-10-28 | Invitrogen Corporation | Method for rapid amplification of DNA |
| WO2003062462A2 (en) | 2002-01-16 | 2003-07-31 | Dynal Biotech Asa | Method for isolating nucleic acids and protein from a single sample |
| US7745180B2 (en) | 2002-04-24 | 2010-06-29 | Hitachi Chemical Co., Ltd. | Device and method for high-throughput quantification of mRNA from whole blood |
| CN1188529C (zh) * | 2002-06-11 | 2005-02-09 | 刘全俊 | 一种基因芯片的纳米金标记银染检测法 |
| US20050059024A1 (en) * | 2003-07-25 | 2005-03-17 | Ambion, Inc. | Methods and compositions for isolating small RNA molecules |
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2004
- 2004-03-09 US US10/796,298 patent/US7745180B2/en not_active Expired - Fee Related
- 2004-10-29 EP EP08014198A patent/EP1997912B1/de not_active Ceased
- 2004-10-29 CN CN200910149381.9A patent/CN101696442B/zh not_active Expired - Fee Related
- 2004-10-29 AT AT04796890T patent/ATE491042T1/de not_active IP Right Cessation
- 2004-10-29 AT AT08014198T patent/ATE466110T1/de not_active IP Right Cessation
- 2004-10-29 AT AT08014199T patent/ATE517193T1/de not_active IP Right Cessation
- 2004-10-29 EP EP04796890A patent/EP1682678B1/de not_active Not-in-force
- 2004-10-29 DE DE602004026947T patent/DE602004026947D1/de active Active
- 2004-10-29 JP JP2006538406A patent/JP4594321B2/ja not_active Expired - Fee Related
- 2004-10-29 AT AT08075176T patent/ATE442458T1/de not_active IP Right Cessation
- 2004-10-29 EP EP08014199A patent/EP2058405B1/de not_active Not-in-force
- 2004-10-29 EP EP08075176A patent/EP1953245B1/de not_active Not-in-force
- 2004-10-29 DE DE602004023143T patent/DE602004023143D1/de active Active
- 2004-10-29 CN CNA2004800323356A patent/CN1997752A/zh active Pending
- 2004-10-29 DE DE602004030472T patent/DE602004030472D1/de active Active
- 2004-10-29 WO PCT/US2004/036309 patent/WO2005042784A2/en active Search and Examination
- 2004-10-29 CN CN2009101493823A patent/CN101696409B/zh not_active Expired - Fee Related
- 2004-10-29 CN CN200910149383A patent/CN101696448A/zh active Pending
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2006
- 2006-03-15 US US11/376,018 patent/US8101344B2/en active Active
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2007
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