WO2000077253A1 - Appareil et procede d'examen des genes - Google Patents
Appareil et procede d'examen des genes Download PDFInfo
- Publication number
- WO2000077253A1 WO2000077253A1 PCT/JP1999/003209 JP9903209W WO0077253A1 WO 2000077253 A1 WO2000077253 A1 WO 2000077253A1 JP 9903209 W JP9903209 W JP 9903209W WO 0077253 A1 WO0077253 A1 WO 0077253A1
- Authority
- WO
- WIPO (PCT)
- Prior art keywords
- filter
- sample preparation
- pcr amplification
- amplification reaction
- opening
- Prior art date
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Definitions
- the present invention relates to a sample preparation apparatus for preparing a sample for performing a gene diagnosis using whole blood or a cell culture solution, a gene test apparatus for easily performing a process from sample preparation to detection of a target arrested child, and a gene.
- a sample preparation apparatus for preparing a sample for performing a gene diagnosis using whole blood or a cell culture solution
- a gene test apparatus for easily performing a process from sample preparation to detection of a target arrested child
- a gene Related to inspection method. Background art
- JP-A-7-98314 discloses a fecal occult blood measuring device.
- the stool sample lysate is first removed of solid components by a separation filter. Thereafter, the stool sample solution from which the solid components have been removed is dropped onto the measurement filter.
- the measurement filter is colored.
- the measurement filter is uncolored. Therefore, the presence or absence of fecal occult blood can be easily determined by visually judging the presence or absence of coloring of the measurement filter. Disclosure of the invention
- a second member in which a plurality of second regions having a second filter holding a CR amplification reaction solution is formed at a lower portion, and a second member in which a plurality of third regions having a hydrophobic third filter are formed.
- a genetic test method using a sample preparation device in which a flow path is formed (1) A buffer solution in which cells are suspended is injected into the first opening, and the cells are captured by a first filter. (2) injecting a denaturing agent into the first opening to capture the polynucleotide eluted from the cells in the second filter; and (3) fluorescently labeling the first opening.
- the first member, the second member, and the third member are arranged in this order from above, and the first region, the second region, and the third member are
- C 20 a flow path connecting an upper opening into which a buffer solution in which cells are suspended is injected, and a lower opening through which waste liquid flows out, wherein the flow path extends from the upper opening to the lower opening.
- FIG. 10 is a cross-sectional view showing an example of the configuration of an arrested child detecting device for detecting the amplification process of a target gene in real time in the second embodiment of the present invention.
- Fig. 11 shows the configuration of an abductor detection device that irradiates a laser to a plurality of sample preparation units from the lateral direction and detects the amplification process of the target gene in real time in the second embodiment of the present invention. It is a perspective view showing an example.
- FIG. 16 is a perspective view showing a configuration example of a sample preparation device having a sample preparation unit for simultaneously processing a plurality of samples in the fifth embodiment of the present invention.
- FIG. 27 is a sectional view showing a configuration example of a sample preparation device according to an eighth embodiment of the present invention.
- Blood collection volume is 1 mL
- the solution 300 passes through the first filter 110 and flows down into the space below the first filter 110, but most of the solution 300 does not pass through the first filter 110 by gravity alone. Then, a pressure is applied from above the sample preparation unit 100 to pass the solution 300 through the first filter I10. At this time, only the white blood cells 302 in the solution 300 are captured by the first filter 110.
- the solution that has passed through the first filter 110 passes through the space below the first filter 110, the second filter 130, and the third filter 140, and flows out to the waste liquid tank. Instead of applying pressure for passing the solution through the first, second, and third filters, centrifugal force generation, suction from the lower opening, etc. can also be used. Subsequently, 3 mL of the denaturing agent 3I0 is poured into the upper space of the first filter 110 (step-2).
- FIG. 5 is a cross-sectional view showing a configuration example of an arrested child detecting device that detects an amplification process of a target gene in real time using an optical fiber in the first embodiment of the present invention.
- the optical fiber 131 is inserted from the upper part of the sample preparation unit 100, pressure is applied to the center of the first filter 110, the filter is pierced, a hole is made, and the fiber 31 is passed through the upper part of the second filter 130. PCR amplification products retained in
- the pretreatment of the sample blood is performed in the same manner as in the first embodiment.
- a third filter force is formed in the upper part, and lower openings 611-1-3, 61-2-3, 613-3, 614-3 are formed in the lower part of each third filter.
- the fourth substrate 640 has through-holes 6 1 1—4, 6 1 2, and 6 1 1—3, 6 1 2—3, 6 13—3, 6 6 1 3-4, 6 14 —4 forces, 'formed.
- the fourth substrate includes the inside and the inside of each second filter.
- a Peltier element for controlling the temperature in the PCR amplification by raising and lowering the temperature of the solution held in the upper space of each second filter and the second filter is embedded.
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/JP1999/003209 WO2000077253A1 (fr) | 1999-06-16 | 1999-06-16 | Appareil et procede d'examen des genes |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
PCT/JP1999/003209 WO2000077253A1 (fr) | 1999-06-16 | 1999-06-16 | Appareil et procede d'examen des genes |
Publications (1)
Publication Number | Publication Date |
---|---|
WO2000077253A1 true WO2000077253A1 (fr) | 2000-12-21 |
Family
ID=14235988
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PCT/JP1999/003209 WO2000077253A1 (fr) | 1999-06-16 | 1999-06-16 | Appareil et procede d'examen des genes |
Country Status (1)
Country | Link |
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WO (1) | WO2000077253A1 (fr) |
Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003031972A1 (fr) * | 2001-10-05 | 2003-04-17 | Bml, Inc. | Plaque de detection |
EP1504121A2 (fr) * | 2002-04-24 | 2005-02-09 | Hitachi Chemical Research Center, Inc. | Dispositif et methode de quantification a haut debit d'arn messagers a partir du sang total |
JP2005295942A (ja) * | 2004-04-15 | 2005-10-27 | Kyocera Corp | 遺伝子反応管およびこれを用いた遺伝子検査装置 |
JP2007529225A (ja) * | 2004-03-16 | 2007-10-25 | アンビオン インコーポレーティッド | 分画された血液白血球からのrnaの抽出のための方法および試薬 |
JP2009510398A (ja) * | 2005-09-26 | 2009-03-12 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | 流体処理方法及び流体処理装置 |
US7745180B2 (en) | 2002-04-24 | 2010-06-29 | Hitachi Chemical Co., Ltd. | Device and method for high-throughput quantification of mRNA from whole blood |
Citations (7)
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JPH07107999A (ja) * | 1993-10-12 | 1995-04-25 | Hitachi Ltd | 遺伝子解析方法及び装置 |
JPH08266267A (ja) * | 1995-03-24 | 1996-10-15 | Becton Dickinson & Co | 複数の液体標本を反応させるための自動化された装置 |
JPH08275800A (ja) * | 1995-03-24 | 1996-10-22 | Becton Dickinson & Co | 核酸増幅方法及び装置 |
WO1997003348A1 (fr) * | 1995-07-13 | 1997-01-30 | Immunological Associates Of Denver | Appareil autonome integrant l'extraction, l'amplification et la detection de l'acide nucleique |
JPH10505497A (ja) * | 1994-09-09 | 1998-06-02 | ナノゲン・インコーポレイテッド | 自動化された分子生物学的診断システム |
JPH10239300A (ja) * | 1997-02-28 | 1998-09-11 | Kagaku Gijutsu Shinko Jigyodan | 全自動遺伝子解析システム |
JPH10257887A (ja) * | 1996-09-30 | 1998-09-29 | Dainippon Printing Co Ltd | 遺伝子解析装置および方法 |
-
1999
- 1999-06-16 WO PCT/JP1999/003209 patent/WO2000077253A1/fr active Application Filing
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH07107999A (ja) * | 1993-10-12 | 1995-04-25 | Hitachi Ltd | 遺伝子解析方法及び装置 |
JPH10505497A (ja) * | 1994-09-09 | 1998-06-02 | ナノゲン・インコーポレイテッド | 自動化された分子生物学的診断システム |
JPH08266267A (ja) * | 1995-03-24 | 1996-10-15 | Becton Dickinson & Co | 複数の液体標本を反応させるための自動化された装置 |
JPH08275800A (ja) * | 1995-03-24 | 1996-10-22 | Becton Dickinson & Co | 核酸増幅方法及び装置 |
WO1997003348A1 (fr) * | 1995-07-13 | 1997-01-30 | Immunological Associates Of Denver | Appareil autonome integrant l'extraction, l'amplification et la detection de l'acide nucleique |
JPH10257887A (ja) * | 1996-09-30 | 1998-09-29 | Dainippon Printing Co Ltd | 遺伝子解析装置および方法 |
JPH10239300A (ja) * | 1997-02-28 | 1998-09-11 | Kagaku Gijutsu Shinko Jigyodan | 全自動遺伝子解析システム |
Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2003031972A1 (fr) * | 2001-10-05 | 2003-04-17 | Bml, Inc. | Plaque de detection |
US7981608B2 (en) | 2002-04-24 | 2011-07-19 | Hitachi Chemical Co., Ltd. | Device and method for high-throughput quantification of MRNA from whole blood |
EP1504121A4 (fr) * | 2002-04-24 | 2005-06-29 | Hitachi Chemical Res Ct Inc | Dispositif et methode de quantification a haut debit d'arn messagers a partir du sang total |
US7745180B2 (en) | 2002-04-24 | 2010-06-29 | Hitachi Chemical Co., Ltd. | Device and method for high-throughput quantification of mRNA from whole blood |
US7939300B2 (en) | 2002-04-24 | 2011-05-10 | Hitachi Chemical Co., Ltd | Device and method for high-throughput quantification of mRNA from whole blood |
US7968288B1 (en) | 2002-04-24 | 2011-06-28 | Hitachi Chemical Co., Ltd. | Device and method for high-throughput quantification of mRNA from whole blood |
EP1504121A2 (fr) * | 2002-04-24 | 2005-02-09 | Hitachi Chemical Research Center, Inc. | Dispositif et methode de quantification a haut debit d'arn messagers a partir du sang total |
US8076105B2 (en) | 2002-04-24 | 2011-12-13 | Hitachi Chemical Research Center, Inc. | Device and method for high-throughput quantification of MRNA from whole blood |
US8101344B2 (en) | 2002-04-24 | 2012-01-24 | Hitachi Chemical Research Center, Inc. | Device and method for high-throughput quantification of mRNA from whole blood |
JP2007529225A (ja) * | 2004-03-16 | 2007-10-25 | アンビオン インコーポレーティッド | 分画された血液白血球からのrnaの抽出のための方法および試薬 |
JP2005295942A (ja) * | 2004-04-15 | 2005-10-27 | Kyocera Corp | 遺伝子反応管およびこれを用いた遺伝子検査装置 |
JP2009510398A (ja) * | 2005-09-26 | 2009-03-12 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | 流体処理方法及び流体処理装置 |
JP4779104B2 (ja) * | 2005-09-26 | 2011-09-28 | キアゲン ゲゼルシャフト ミット ベシュレンクテル ハフツング | 流体処理方法及び流体処理装置 |
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