EP1557222A2 - Pipette avec éjecteur d'embouts de pipette - Google Patents

Pipette avec éjecteur d'embouts de pipette Download PDF

Info

Publication number
EP1557222A2
EP1557222A2 EP05000212A EP05000212A EP1557222A2 EP 1557222 A2 EP1557222 A2 EP 1557222A2 EP 05000212 A EP05000212 A EP 05000212A EP 05000212 A EP05000212 A EP 05000212A EP 1557222 A2 EP1557222 A2 EP 1557222A2
Authority
EP
European Patent Office
Prior art keywords
spring
pipetting device
drive
pipetting
ejector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP05000212A
Other languages
German (de)
English (en)
Other versions
EP1557222B1 (fr
EP1557222A3 (fr
Inventor
Herbert Belgardt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Priority to EP10011574.0A priority Critical patent/EP2263800A3/fr
Publication of EP1557222A2 publication Critical patent/EP1557222A2/fr
Publication of EP1557222A3 publication Critical patent/EP1557222A3/fr
Application granted granted Critical
Publication of EP1557222B1 publication Critical patent/EP1557222B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/08—Ergonomic or safety aspects of handling devices
    • B01L2200/087—Ergonomic aspects
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/025—Displaying results or values with integrated means
    • B01L2300/026—Drum counters

Definitions

  • the invention relates to a pipetting device with at least one approach for attaching a pipette tip and a discharge device for releasing a Pipette tip from the neck.
  • Pipetting devices are used especially in the laboratory for dosing liquids used. These are taken up in pipette tips through a tip opening and spent.
  • air cushion pipettes is a displacement device for a gas integrated into the pipetting device and communicating through the approach with the Pipette tip connected.
  • the displacement device is an air cushion displaced so that liquid sucked into the pipette tip and ejected from it becomes.
  • the displacement device is usually a cylinder with a movable therein Piston.
  • Pipette tips are detachably connected to the neck to keep them in after use can be replaced with a fresh pipette tip. This can be at subsequent dosing contaminations are avoided.
  • Pipette tips for the one-time use are available inexpensively from plastic.
  • the approach for attaching pipette tips is often a cylindrical or conical projection with respect to a base body or a housing, on the one Pipette tip can be clamped with a matching push-on opening or receptacle is. This can be done without touching the pipette tip by pressing the projection into the Plug-in opening happen to be provided in a holder pipette tip.
  • a discharge device with a drive device and an ejector.
  • the ejector By Actuating the drive means, the ejector is displaced so that it the pipette tip from the approach solves, without this must be handled by the user.
  • the drive device has a mechanism that by means of an actuating button must be manually operated to release the pipette tip from the approach.
  • drive devices with an electric motor drive. The Loosening the pipette tip from the neck can require considerable effort especially with pipette tips clamped firmly on the neck.
  • single-channel systems i.e.
  • Pipetting devices that provide a single approach to a single Pipette tip, this may be the ejection of the pipette tip from the approach make it difficult or impossible.
  • Particularly high expenditure of force can be achieved in multi-channel pipetting systems, the multiple parallel approaches for attaching pipette tips have to be required due to multiple peak ejection forces.
  • EP 0 992 288 A2 is a pipetting system with an axially movable Ejector for releasing a pipette tip from a hub, drive means for driving the axial movements of the ejector and an axial drive movement the drive device transmit in an axial movement of the ejector Anlagenstoff-, pressure medium or linkage known. From the ejector to the Force applied by the pipette tip exceeds the force applied by the user, whereby the throwing off is facilitated.
  • a pipetting device which is an approach to Attaching a pipette tip having a protruding from a shaft Section of a shaft which is rotatable in the shaft.
  • the pipetting device has a rotary drive for the shaft.
  • the end facing the approach is the Shank designed as a curved surface.
  • the pipette tip points at the insertion end also a curve surface.
  • the invention is based on the object, a pipetting device to provide that the force required for the operation of the discharge device reduced.
  • a pipette tip by axial Insertion of the approach plugged into the plug-in opening This is the Aufsteckkraft initiated into the spring. If the attachment force exceeds a certain value, the spring is elastically deformed until the pipette tip clamped onto the neck bumps against the stop. As soon as the pipette tip touches the stop, you can they will not be deferred on the approach anymore. As a result, the Tarsteckkraft limited the pipette tip.
  • the Aufsteckkraft is through the spring characteristic the spring or any existing bias of the spring determined.
  • the feather is designed and optionally biased so that the pipette tip is exactly when sitting with the desired attachment force on the neck, at the stop is applied.
  • the Aufsteckkraft is set so that the pipette tip safely on the Approach sits or seals. Since a precise defined Aufsteckkraft is achieved, it is possible to limit the Aufsteckkraft to a low value at which the required Safety of the seat and the seal is just given. Thus, the Aufsteckkraft compared to conventional pipetting significantly reduced in which, due to the uncertainties of putting on a regular excessive Aufsteckkraft is expended. Accordingly, in the inventive Pipetting the effort required for the operation of the ejection device reduced. In manually operable ejection devices are by Reduction of operating forces the tendon and muscle strain of the user reduced. Electromotive operated ejection devices come with motors lower power.
  • the spring is designed and / or biased that the approach of a defined Aufsteckkraft by Attaching a pipette tip can be applied to the approach, in such a way to Stop is displaced that the pipette tip abuts the stop.
  • the neck and ejector are relative axially and / or rotationally movable.
  • the invention relates to a, in where the ejector is stationary with respect to the pipetting and the Drive device exclusively moves the approach. Furthermore, the invention relates Implementations in which the drive means the ejector and the approach emotional. In preferred embodiments, the approach is with respect to the pipetting device stationary and the drive device is only operatively connected to the ejector, so that only the ejector is movable by means of the drive means.
  • the approach is supported directly on the spring.
  • the Connection of the approach with a permanently connected to the main body displacement device can be done by means of a flexible connection, e.g. with help of a flexible hose or a telescopic pipe connection.
  • the approach is fixed with a displacement device - e.g. with a Cylinder of a relative to the body axially displaceable piston-cylinder device - connected.
  • the displacement device is then with the approach relocated.
  • the displacement of the displacement device can be controlled by the drive device be compensated for the displacement device.
  • the cylinder is supported by the spring on the body, so that the Approach indirectly cushioned over the cylinder.
  • the spring is extended in the axial direction of the neck Coil spring.
  • the coil spring is preferably coaxial with the extension.
  • the displacement device or drive device for the displacement device can be advantageously passed through the coil spring.
  • the pipetting device can be executed with a non-preloaded spring. Then it will be the spring corresponding to the increase in Aufsteckkraft when attaching the approach compressed to the pipette tip.
  • the spring has a spring bias. As a result, it comes only to an axial displacement of the approach when the Aufsteckkraft exceeds the force to bias the spring. This will be the for defined the reaching of the stop required Aufsteckkraft. It also favors This particularly compact spring arrangements and short displacement paths of the Approach.
  • the stop on which the pipette tip when plugging meets firmly connected to the body.
  • the stop is axially movable with respect to the approach and a fixed to the Body connected limit for the axial movement of the stop to Base body available.
  • the stop is formed on the ejector.
  • the stop At a Execution of the ejector as the approach coaxial sleeve is the stop by the formed the approach facing the end of the sleeve.
  • the approach is different ways in its initial axial position for recording a pipette tip fixed, for example by connecting the approach with the spring.
  • the starting position of the approach is then through the starting position defines the spring unloaded by the approach.
  • the Approach in the direction of the spring away supported on another stop, against which he is pressed by the spring.
  • the starting position is precisely determined.
  • no connection between approach and spring is required.
  • the spring is exchangeable and / or is a device for adjusting the spring preload available. This is an adaptation of Push-on and ejection forces on differently shaped or sized pipette tips possible. The range of application of the pipetting device is thereby increased.
  • the drive device is against the action of a on the one hand on a movable part of the drive means and on the other hand on Main body supported further spring operable.
  • the drive device returns after actuation automatically back to their original position.
  • a defined Starting position is e.g. with respect to the body fixed limit for the Movement of the ejector or the drive device available. This can at the same time the limit for the axial movement of the stop to the body out be.
  • the base body on a support or frame.
  • the main body has a housing, optionally with a carrier or frame contained therein.
  • the pipetting device is a hand-held device and / or a stationary device and / or an electrically powered device and / or a (Semi) automatic.
  • the pipetting device of Hand led to the location of the sample taking and sample deliveries and becomes the Pickup and delivery of liquid and the operation of the discharge device controlled by hand.
  • the drive means for the displacement device and / or the ejector are designed mechanically and / or electromechanically. The latter also applies to the execution of the pipetting as a stationary device.
  • the pipetting device When the pipetting device is designed as a (semi) automaton, all Functions or part of the functions of the pipetting devices (recording and Dispensing liquid, moving the pipetting devices into positions for Picking up and dispensing liquid or pipette tips, picking up and dispensing from pipette tips) automatically.
  • the pipetting device has a number of parallel Approaches for receiving pipette tips. This is a Multichannel pipetting. Due to the multiplied peak ejection forces Here, the use of the invention particularly advantageous.
  • the approaches associated with a common ejector are associated with the approaches associated with a common ejector.
  • the displacement device and the drive device for driving the displaceable limitation of the pipetting device are easily achieved by Mating along a longitudinal axis of the bayonet connection and turning around the longitudinal axis of the bayonet connection connected to each other or in reverse Way separable from each other.
  • the bayonet connection will be simultaneously the operative connection between the drive member and the displaceable boundary is established, without the need for special further actions.
  • the active compound is solved without special further actions.
  • the invention enables a particularly simple, fast and secure connection and separating the displacing means and the driving means for driving the movable limit, for example, during assembly, before autoclaving or other cleaning of the lower part, before replacing the lower part to For the purpose of changing the work area, repair, etc.
  • the Bayonet connection is particularly susceptible to interference.
  • the drive device for driving the displaceable boundary can be configured in different ways. It has technical means, that To move drive member so that this is the movable limit of Displaced displacement device.
  • the drive member carries e.g. a Linear motion off.
  • the drive means for driving the movable boundary on a linear drive are e.g. around a directly operable by pressing a button manually stroke rod or one over an electric drive motor and a transmission linearly displaceable lifting rod.
  • a pneumatically or hydraulically operated Pressure medium cylinder as a drive for the lifting rod, via a pneumatic or hydraulic control and a pressure medium reservoir is actuated. If that Drive organ performs no linear movement, but a spatial Feed motion, the drive means for driving the displaceable Limiting a corresponding drive.
  • the drive means for driving the displaceable boundary a housing in which drive and drive member are arranged.
  • the drive member is a parallel to the longitudinal axis of the Bayonet connection displaceable lifting rod of the drive device and has the Displacement device connected to the boundary, transverse to the lifting rod directed contact surface, by a lifting spring against the end of the lifting rod is pressed.
  • the operative connection between the drive member and movable limit automatically when making the bayonet connection made and automatically released when dissolving the bayonet connection.
  • the contact surface is connected to a via a rod with the displaceable boundary connected pressure piece formed and is the lifting spring designed as a helical spring, which at one end on the pressure piece and the other at the Displacement chamber is supported.
  • the bayonet connection can be configured in various ways. Involved by The invention is in particular the embodiment of the drive device as a male Part and the displacement device as a female part of the bayonet connection and vice versa.
  • the drive device a cylindrical receptacle having at one end an opening through which the cylindrical receptacle in the axial direction is accessible from the outside, the at least having an axially directed longitudinal groove, with a circumferential direction of the cylindrical Receiving directional annular groove is connected, and has the displacement device on a cylindrical portion at least one outwardly projecting Projection, wherein the cylindrical portion in the axial direction of the cylindrical Recording through the opening in the receptacle and with the projection in the Longitudinal groove can be inserted and screwed with the projection in the annular groove.
  • the drive means the female and the displacement device the male part.
  • the annular groove at a distance from the longitudinal groove a in the axial direction of the recording extended boundary wall, up to the Projection is rotatable. The achievement of the limit indicates to the user that the Bayonet connection is made.
  • the annular groove is at a distance from the longitudinal groove a parallel thereto extending L Lucassnutabexcellent connected in a Distance from the opening ends.
  • the annular groove has a ramp-like boundary wall, their distance from the opening with increasing distance from the Longitudinal groove increases.
  • the ramp-like course of the boundary wall facilitates the Finding the connection position and separating the displacement of the drive device.
  • the longitudinal groove, the annular groove and optionally the L jossnutabrough formed in a cylindrical coupling piece, which is the receptacle of Drive device forms and is fixed in this. This will produce, Assembly and disassembly easier.
  • the drive device on a spring which against the Displacement device connected to the drive device via the bayonet connection suppressed. This will secure the bayonet connection.
  • the spring is at a further opening of the receptacle arranged, that of the opening for axial insertion of the displacement device opposite. Through the opening displacement and spring act on each other one.
  • the spring is a helical spring which is supported on an inner end face of the coupling piece.
  • the longitudinal groove and / or annular groove and / or the Leksnutabrough opened for further opening.
  • the displacement device is a piston-cylinder device with a cylinder and a piston displaceable therein and has the Piston on the movable limit.
  • Other displacement devices are also included by the invention, for example, a displacement chamber with an elastic wall forming the displaceable boundary.
  • a piston-cylinder device is e.g. operated by a linear drive device.
  • a corresponding actuation is in a displacement chamber with an elastic Wall possible.
  • the latter can also be a drive device with a controlled spatial drive movement. So it is possible, for example, the elastic wall by action of a hydraulic or pneumatic Control pressure medium outside.
  • the approach is coaxial with the longitudinal axis of the bayonet connection aligned. According to a further embodiment, the approach is fixed with connected to the displacement device.
  • the pipetting device has a discharge device for Discard a pipette tip from the hub, the one at the drive device arranged discharge drive, one arranged on the displacement device Ejector and a directed in the direction of the longitudinal axis of the bayonet connection, having releasable axial clamping connection between the ejection drive and ejector.
  • the Clamping is simultaneous with the manufacture of the bayonet connection with the Phase of the axial pushing together of displacing device and drive device can be produced and released in the opposite direction.
  • the ejection drive one of the drive device parallel to the bayonet connection projecting discharge rod and the ejector a to Approach parallel, axial bore, with which the discharge rod is press-connected.
  • the ejector is guided on the displacement device.
  • the ejector is one on the displacement device guided sleeve.
  • the pipetting device is a hand-held device and / or a stationary device and / or an electrically powered device and / or a (Semi) automatic.
  • the pipetting device of Hand led to the location of the sample taking and sample deliveries and becomes the Pickup and delivery of liquid and the operation of the discharge device controlled by hand.
  • the drive means for the displacement device and / or the ejector are designed mechanically and / or electromechanically. The latter also applies to the execution of the pipetting as a stationary device.
  • the pipetting device When the pipetting device is designed as a (semi) automaton, all Functions or part of the functions of the pipetting devices (recording and Dispensing liquid, moving the pipetting devices into positions for Picking up and dispensing liquid or pipette tips, picking up and dispensing from pipette tips) automatically.
  • the pipetting device has a number of parallel Approaches for receiving pipette tips.
  • This is a Multichannel pipetting.
  • Each batch of the pipetting device is a separate one Displacement device or a common assigned, via a bayonet connection is connected to the drive device. It can be a act common drive device for all displacement devices.
  • the pipetting device according to FIGS. 1 and 2 has an elongated handle shaped Housing 1 with an upper housing part 2 and a lower housing part 3. Das Upper housing part 2 with all parts contained forms a drive device and the lower housing part 3 with all parts contained a displacement device.
  • the upper housing part 2 forms a base body. It has one upstairs Screw cap 4. From this stands up an adjusting sleeve 5.
  • the adjusting sleeve 5 is axially immovable and rotatably mounted in the upper housing part 2.
  • a push button 6 is arranged, which still further upwards protrudes.
  • the push button 6 is connected to a lifting rod 7, which in the upper housing part. 2 is passed through a spindle 8.
  • the spindle 8 is in an internal thread 9 of a Screwed in the upper housing part 2 bearing body 10 is screwed.
  • the spindle 8 has a rotatably connected to her driver 11 above.
  • the Driver 11 has on the circumference two diametrically opposed radial projections 12.
  • the radial projections 12 engage in - not shown - axially extending Grooves of the adjusting sleeve 5 a.
  • the spindle 8 has at the bottom an end stop 13 in the form of radially outwardly projecting Ribs. In the position shown, the end stop 13 is a small piece below a shoulder 14 of the bearing body 10 with which it cooperates.
  • the lifting rod 7 has a flange 15, which in the position shown at the bottom of the Spindle 8 is applied.
  • a spring plate 16 is arranged, with a Collar 17 engages in the bearing body 10.
  • the spring plate 16 has an axial bottom protruding, sleeve-shaped bearing portion 18 through which the lifting rod 7 passed is.
  • the pipetting device on a spring, not shown, which the lifting rod 7 presses upward, so that the flange 15 at the bottom of the spindle. 8 is applied.
  • a coil spring between flange 15 and spring plate 16th arranged.
  • a coupling piece in the housing 19th attached at a distance below the spring plate 16 at a distance below the spring plate 16 at a distance below the spring plate 16 is a coupling piece in the housing 19th attached.
  • This has several pockets inside 20. These have an axial over the Total length of the coupling piece 19 extended longitudinal groove 21. Furthermore, they have at the top End of the coupling piece 19 a over a small part of the circumference of the coupling piece 19 extended annular groove 22. This has at a distance from the upper end of the Coupling piece 19 below a ramp-like boundary wall, the starting from the longitudinal groove 21 increasingly approximated to the upper end of the coupling piece 19 is. Finally, the pockets 20 at the other end of the annular groove 22 a short axial longitudinal groove portion 23 spaced at a distance from the upper end of Coupling piece 19 ends in the coupling piece 19.
  • spring 24 under bias arranged, which is designed as a helical spring.
  • the adjusting sleeve 5 has on the circumference a sprocket 25 which is connected to a gear 26th cooperates, a counter 27 with several superimposed on an axis 28th arranged Zumblelwerkssonn 29 drives.
  • the counter wheels 29 each have Numerals from 0 to 9, the lower Zählwerksrad 29 is driven by the gear 26.
  • the arranged above it Zählwerksson 29 are further rotated by one digit, when the underlying Zählwerksrad 29 passes from 9 to 0.
  • the lower housing part 3 can be detachably connected to the upper housing part 2. For this purpose points the lower housing part 3 on the jacket of an upper, cylindrical portion 30th a plurality of outwardly projecting ribs 31 which extend in the axial direction of the cylindrical Section 30 extend.
  • the lower housing part 3 has a plurality of below the cylindrical portion 30 conical sections 31 to 33 of different length and taper coming from the Drawing to emerge.
  • the conical section 33 is down with a long, light conical projection 34 connected to attach a pipette tip. This one has turn down a short, more conical Aufsteckende 35.
  • the lower housing part 3 houses a displacement device in the form of a Piston-cylinder unit 36. This has a arranged in the conical portion 32 Cylinder 37, in which a piston 38 dips. The piston 38 is above a piston rod 39 connected to a pressure piece 40. The piston 38 forms a displaceable Limitation of the cylinder 37.
  • the lower housing part 3 has a piston holder 41, the the cylindrical portion 30 bridged above.
  • the piston holder 41 has a top one central passage 42 through which a lower portion of the reciprocating piston 7 axially can be passed.
  • a Lifting spring 43 arranged, which is designed as a helical spring. By the lifting spring 43 the piston 38 and the piston rod 39 are passed.
  • the lifting spring 43 is biased and presses the pressure piece 40 against the piston holder 41, so that the piston 38 is pulled out of the cylinder 37 maximum.
  • a connecting passage 44 passes through the boss 34 and connects the cylinder 37 with a muzzle in Aufsteckende 35.
  • the pipetting device has a discharge device 45.
  • the discharge device 45 has in the upper housing part 2 next to the knob 5, an operating button 46th
  • the operating knob 46 is connected to a discharge rod 47 which is parallel to Lifting rod 7 passes through the upper housing part 2 therethrough.
  • a transmission 48 is integrated in the discharge rod 47.
  • the transmission 48 sets a axial actuating stroke of the actuating knob 46 in a smaller drive stroke with increased strength.
  • Suitable transmissions 48 are described in EP 0 992 288 A, and Although generally in the general part of the description and especially in the figure description, which are incorporated by reference into the present application.
  • the discharge rod 47 is connected via a further coil spring 49 in the upper housing part. 2 supported, so that the actuating button 46 is pressed into the starting position shown is in which he is pressed against the action of the other coil spring 49.
  • the lower end of the discharge rod 47 protrudes into a receptacle 50 at the lower end of the Housing top 2 into it.
  • the discharge device 45 has on the lower housing part 3 a discharge sleeve 51. This is on the cylindrical portion 30, the conical portion 32 and the projection 34th guided. Accordingly, the contour of the discharge sleeve 51 is the contours of the aforementioned Sections of the lower housing part 3 aligned. It has the Abschhülse 51 inside stages 52, 53, the postponement of the ejection sleeve 51 after above limit, by at conical sections 31, 33 of the housing lower part 3rd issue.
  • the pipetting device can be used as follows:
  • the lifting rod engages 7 through the passage 42 through and lies with its lower end to the Pressure piece 40 on.
  • a pipette tip 56 is clamped on the lower end of the projection 34.
  • the pipette tip 56 has a lower tip opening 57 for receiving and dispensing of liquid.
  • the push button 6 For pipetting the push button 6 is pressed down, so that the piston 38th Air displaced from the cylinder 37. Then the pipette tip 56 with its lower Tip opening 57 immersed in the liquid to be pipetted. After that, the Push button 6 released and the lifting rod 7 is under spring action in her Starting position back. Likewise, the piston 38 returns under the action of the spring 43 back to its starting position. Here, the piston 38 sucks liquid through the lower tip opening 57 into the pipette tip 56.
  • the lower tip opening 57 of the pipetting device becomes a discharge site aligned.
  • the liquid contained in the pipette tip 56 is pressed in the push button 6, re-immersion of the piston 38 in the cylinder 37th and expelling air through the connecting channel 44 delivered. After releasing of the actuating knob 6 drive the lifting rod 7 and the piston 38 by spring force back to the starting position.
  • a dispensing head 61 has a multi-channel pipetting device Housing 62, in the eight parallel piston-cylinder devices 63 in a row are arranged. Each piston-cylinder device 63 has a cylinder 64, in the one Plunger 65 is immersed.
  • each cylinder 64 designed as a helical spring spring 66 is guided, the bottom rests on a circumferential projection 67 of the cylinder 64.
  • the cylinder 64 In the area of the circumferential projection 67, the cylinder 64 is in a guide rail 68 led.
  • each cylinder 64 is below the circumferential projection 67 with a conical projection 69 connected to attach a pipette tip 70.
  • the cylinder 64 by a fixed in the housing 62nd arranged support plate 71 passed.
  • the springs 66 are supported on the underside the support plate 71 from.
  • the cylinders 64 have a further circumferential projection above, which is a spring bearing 72 forms.
  • Each spring bearing 72 is engaged by a console, not shown, the inside of the wall of the housing 62 protrudes.
  • All pistons 65 are at the top in a piston receptacle 73 in the form of a traverse held.
  • the piston receptacle 73 has a pin 74 at the top for connection to a Not shown lifting rod, which leads to a drive for the piston 65.
  • pistons 65 On all the pistons 65 are further springs 75 designed as helical springs out, which are supported at the bottom of the spring bearing 72 and at the top at the bottom support the piston seat 73.
  • an ejector 76 in the form of an angled plate stored. This has in the lower, horizontal leg 77 eight holes through which the Approaches 69 are passed.
  • the vertical leg 78 is above a shaft 79 connectable with a discharge bar, not shown.
  • the pistons 65 are guided by a piston seal 80 in the cylinder 64.
  • the dispensing head 61 is connected to the lifting rod and the discharge rod of a not shown Drive device connected.
  • the pistons 65 are connected by means of the pin 74 Lifting rod moves.
  • the shaft 79 by means of the lifting rod moved down so that the ejector 76 is moved down and with its leg 77, the pipette tips 70 from the lugs 69 depressed.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
EP05000212.0A 2004-01-21 2005-01-07 Pipette avec éjecteur d'embouts de pipette Active EP1557222B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP10011574.0A EP2263800A3 (fr) 2004-01-21 2005-01-07 Pipette avec éjecteur d'embouts de pipette

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102004003433 2004-01-21
DE102004003433A DE102004003433B4 (de) 2004-01-21 2004-01-21 Pipettiervorrichtung mit einer Abwurfeinrichtung für Pipettenspitzen

Related Child Applications (2)

Application Number Title Priority Date Filing Date
EP10011574.0A Division-Into EP2263800A3 (fr) 2004-01-21 2005-01-07 Pipette avec éjecteur d'embouts de pipette
EP10011574.0A Division EP2263800A3 (fr) 2004-01-21 2005-01-07 Pipette avec éjecteur d'embouts de pipette

Publications (3)

Publication Number Publication Date
EP1557222A2 true EP1557222A2 (fr) 2005-07-27
EP1557222A3 EP1557222A3 (fr) 2007-05-09
EP1557222B1 EP1557222B1 (fr) 2014-11-12

Family

ID=34625769

Family Applications (2)

Application Number Title Priority Date Filing Date
EP05000212.0A Active EP1557222B1 (fr) 2004-01-21 2005-01-07 Pipette avec éjecteur d'embouts de pipette
EP10011574.0A Withdrawn EP2263800A3 (fr) 2004-01-21 2005-01-07 Pipette avec éjecteur d'embouts de pipette

Family Applications After (1)

Application Number Title Priority Date Filing Date
EP10011574.0A Withdrawn EP2263800A3 (fr) 2004-01-21 2005-01-07 Pipette avec éjecteur d'embouts de pipette

Country Status (3)

Country Link
US (1) US7434484B2 (fr)
EP (2) EP1557222B1 (fr)
DE (1) DE102004003433B4 (fr)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2165765A1 (fr) 2008-09-12 2010-03-24 Eppendorf Ag Dispositif de pipetage avec filetage double
EP2210667A1 (fr) * 2009-01-19 2010-07-28 Eppendorf Ag Pipette avec éjecteur de pointe
CN106470760A (zh) * 2014-07-04 2017-03-01 埃佩多夫股份公司 用于操作注射器的移液器

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CH708024B8 (de) * 2005-12-20 2015-01-15 Tpp Techno Plastic Products Ag Verfahren zur Herstellung einer Pipette.
US20090071267A1 (en) * 2007-09-17 2009-03-19 Greg Mathus Pipette tip ejection mechanism
US9470616B2 (en) 2009-04-27 2016-10-18 E.I. Spectra, Llc Pipette instrument
DE102009051654B4 (de) * 2009-10-30 2013-01-03 Eppendorf Ag Dosiervorrichtung für Flüssigkeiten und Verfahren zum Dosieren von Flüssigkeiten
JP2011115759A (ja) * 2009-12-07 2011-06-16 Fukae Kasei Kk ピペット装置
WO2011148432A1 (fr) * 2010-05-28 2011-12-01 栄研化学株式会社 Pipette
DE102010047126A1 (de) * 2010-10-04 2012-04-05 Eppendorf Ag Pipette
EP2633913B1 (fr) 2012-03-01 2019-05-08 Eppendorf AG Dispositif de pipetage et dispositif de pipetage multi-canal
US9358538B2 (en) 2012-04-30 2016-06-07 The Regents Of The University Of Michigan High resolution pipette
PL2735369T3 (pl) * 2012-11-23 2017-09-29 Eppendorf Ag Pipeta wielokanałowa
US9815053B2 (en) * 2013-01-15 2017-11-14 Mettler-Toledo Rainin, LLC Liquid end assembly for a multichannel air displacement pipette
USD772426S1 (en) 2014-01-13 2016-11-22 Gilson, Inc. Pipette system cartridge
FR3040896B1 (fr) * 2015-09-15 2017-10-13 Gilson Sas Pipette de prelevement comprenant un organe de commande a double fonction d'ejection de cone et de deverrouillage du systeme de reglage de volume
CN108136399B (zh) 2015-10-13 2021-06-01 豪夫迈·罗氏有限公司 用于处理样品或试剂的设备的移液装置、用于处理样品或试剂的设备和将样品或试剂移液的方法
PL3260822T3 (pl) 2016-06-21 2021-02-08 Eppendorf Ag Wielokanałowa strzykawka do stosowania z urządzeniem dozującym
DE102016111912A1 (de) 2016-06-29 2018-01-04 Eppendorf Ag Dosierkopf, Dosiervorrichtung umfassend einen Dosierkopf und Verfahren zum Dosieren mittels eines Dosierkopfes
DE102016111910A1 (de) 2016-06-29 2018-01-04 Eppendorf Ag Dosierkopf, Dosiervorrichtung umfassend einen Dosierkopf und Verfahren zum Dosieren mittels eines Dosierkopfes
DE102016121816A1 (de) * 2016-11-14 2018-05-17 Ika-Werke Gmbh & Co. Kg Fluidabgabeeinheit und Handdosiervorrichtung mit wenigstens einer Fluidabgabeeinheit
JP6865510B2 (ja) * 2017-06-01 2021-04-28 株式会社エー・アンド・デイ マルチチャンネルピペット
US10744498B2 (en) * 2017-09-19 2020-08-18 Mettler-Toledo Rainin, LLC Pipette quickset volume adjustment mechanism
EP3477307B1 (fr) * 2017-10-24 2020-07-22 F. Hoffmann-La Roche AG Dispositif de pipettage et système de positionnement de dispositif de pipettage
EP3666379A1 (fr) 2018-12-14 2020-06-17 Eppendorf AG Tête de pipettage, dispositif de pipettage comprenant une tête de pipettage et procédé de pipettage à l'aide d'une tête de pipettage
EP3851191A1 (fr) * 2020-01-17 2021-07-21 Eppendorf AG Procédé de fonctionnement d'une pipette à course de piston, pipette à course de piston, appareil et système de traitement des données
CA3218868A1 (fr) 2020-02-14 2021-08-19 DeNovix, Inc. Pipette a plage volumetrique etendue dynamique
CN111744567B (zh) * 2020-06-24 2021-03-30 巨爱宁 一种化验科室用移液枪
TWI824896B (zh) * 2022-08-18 2023-12-01 創茂生技股份有限公司 旋轉式移液裝置
TWI840043B (zh) * 2022-12-22 2024-04-21 緯創資通股份有限公司 多功能移液器及自動化檢測機器

Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4086062A (en) * 1977-02-23 1978-04-25 Hach Chemical Company Digital titration device
US4567780A (en) * 1984-03-12 1986-02-04 American Hospital Supply Corporation Hand-held pipette with disposable capillary
EP0289946A2 (fr) * 1987-05-02 1988-11-09 Teruaki Itoh Appareil de distribution d'échantillons de liquides
US5104624A (en) * 1989-10-20 1992-04-14 Costar Corporation Pipetter
WO1998031465A1 (fr) * 1997-01-17 1998-07-23 Matrix Technologies Corporation Dispositif de pipetage comportant un indicateur, et son mode d'utilisation
EP1136127A2 (fr) * 2000-03-20 2001-09-26 Brand Gmbh + Co Kg Dispositif de pipetage à canaux multiples et tige de pipette associée
US20020001545A1 (en) * 2000-06-26 2002-01-03 Cronenberg Richard A. Automatic pipette identification and detipping
WO2002016036A2 (fr) * 2000-08-21 2002-02-28 Mwg-Biotech Ag Tete de pipettage pour robot comportant plusieurs pointes de pipettes

Family Cites Families (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2287941A1 (fr) * 1974-10-15 1976-05-14 Marteau D Autry Eric Dispositif d'ejection de l'embout amovible d'une pipette
US4009611A (en) * 1976-05-13 1977-03-01 Oxford Laboratories Inc. Hand-held micropipettor with improved pipette tip ejector
DE2711124C2 (de) * 1977-03-15 1979-05-10 Labora Mannheim Gmbh Fuer Labortechnik, 6800 Mannheim Handpipette
US4187724A (en) * 1978-01-11 1980-02-12 Indicon Inc. Replaceable tip for a pipette
US4824641A (en) * 1986-06-20 1989-04-25 Cetus Corporation Carousel and tip
JPH0434456Y2 (fr) * 1987-05-02 1992-08-17
JP2701900B2 (ja) * 1988-12-20 1998-01-21 株式会社ニチリョー マルチピペット
FI921765A0 (fi) * 1992-04-21 1992-04-21 Labsystems Oy Med en spetsavlaegsnare foersedd pipett.
FI90207C (fi) * 1992-05-04 1994-01-10 Wallac Oy Pipettilaitteisto
US5614153A (en) * 1995-05-26 1997-03-25 Rainin Instrument Co., Inc. Pipette tip ejector
US5700959A (en) * 1995-07-14 1997-12-23 Rainin Instrument Co., Inc. Manual pipette with magnet assist
US6499363B1 (en) * 1997-12-12 2002-12-31 The Sailor Pen Co., Ltd. Tip for pipette and pipette with the same
FI107025B (fi) * 1998-06-10 2001-05-31 Biohit Oyj Imulaite ja menetelmä kertakäyttökärjen irroittamiseksi
PL190746B1 (pl) 1998-08-05 2006-01-31 P Z Htl Sa Urządzenie do odrzucania wymiennej końcówki w pipecie
DE19835833A1 (de) * 1998-08-07 2000-02-17 Max Planck Gesellschaft Dosierkopf zur parallelen Bearbeitung einer Vielzahl von Fluidproben
DE19845950C1 (de) * 1998-10-06 2000-03-23 Eppendorf Geraetebau Netheler Pipettiersystem mit einer Pipettiervorrichtung und mindestens einer daran lösbar befestigten Pipettenspitze
US6168761B1 (en) * 1998-11-06 2001-01-02 Rainin Instrument Co., Inc. Pipette with improved pipette tip and mounting shaft
US6197259B1 (en) * 1998-11-06 2001-03-06 Rainin Instrument Co., Inc. Easy eject pipette tip
US6833114B1 (en) * 1999-05-18 2004-12-21 Socorex Isba S.A. Pipette with tip ejector
US6532837B1 (en) * 2000-02-03 2003-03-18 Rainin Instrument, Llc Pipette device with tip ejector utilizing stored energy
US6428750B1 (en) * 2000-02-17 2002-08-06 Rainin Instrument, Llc Volume adjustable manual pipette with quick set volume adjustment
FR2807340B1 (fr) * 2000-04-07 2002-12-06 Gilson Sa Pipette de prelevement d'echantillons liquides a mecanisme d'ejecteur de cone
PL192826B1 (pl) 2000-06-07 2006-12-29 P Z Htl Sa Moduł pipety wielokanałowej
JP3719375B2 (ja) * 2000-07-26 2005-11-24 ニプロ株式会社 溶液調製装置
FI20002241A0 (fi) * 2000-10-11 2000-10-11 Labsystems Oy Säädettävä pipetti
FI20010972A0 (fi) * 2001-05-09 2001-05-09 Thermo Labsystems Oy Kärkisäiliöpipetti
DE10135963B4 (de) * 2001-07-24 2005-09-29 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Vorrichtung zum Pipettieren einer Flüssigkeit
WO2003033151A1 (fr) * 2001-10-16 2003-04-24 Matrix Technologies Corporation Pipette manuelle
US6967004B2 (en) * 2001-10-30 2005-11-22 Rainin Instrument, Llc Pipette with improved pipette tip and mounting shaft

Patent Citations (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4086062A (en) * 1977-02-23 1978-04-25 Hach Chemical Company Digital titration device
US4567780A (en) * 1984-03-12 1986-02-04 American Hospital Supply Corporation Hand-held pipette with disposable capillary
EP0289946A2 (fr) * 1987-05-02 1988-11-09 Teruaki Itoh Appareil de distribution d'échantillons de liquides
US5104624A (en) * 1989-10-20 1992-04-14 Costar Corporation Pipetter
WO1998031465A1 (fr) * 1997-01-17 1998-07-23 Matrix Technologies Corporation Dispositif de pipetage comportant un indicateur, et son mode d'utilisation
EP1136127A2 (fr) * 2000-03-20 2001-09-26 Brand Gmbh + Co Kg Dispositif de pipetage à canaux multiples et tige de pipette associée
US20020001545A1 (en) * 2000-06-26 2002-01-03 Cronenberg Richard A. Automatic pipette identification and detipping
WO2002016036A2 (fr) * 2000-08-21 2002-02-28 Mwg-Biotech Ag Tete de pipettage pour robot comportant plusieurs pointes de pipettes

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2165765A1 (fr) 2008-09-12 2010-03-24 Eppendorf Ag Dispositif de pipetage avec filetage double
DE102008048252A1 (de) 2008-09-12 2010-04-15 Eppendorf Ag Pipettiervorrichtung
US8297134B2 (en) 2008-09-12 2012-10-30 Eppendorf Ag Pipetting device
EP2210667A1 (fr) * 2009-01-19 2010-07-28 Eppendorf Ag Pipette avec éjecteur de pointe
CN106470760A (zh) * 2014-07-04 2017-03-01 埃佩多夫股份公司 用于操作注射器的移液器
CN106470760B (zh) * 2014-07-04 2019-06-07 埃佩多夫股份公司 用于操作注射器的移液器
US10507463B2 (en) 2014-07-04 2019-12-17 Eppendorf Ag Pipette for activating a syringe

Also Published As

Publication number Publication date
EP1557222B1 (fr) 2014-11-12
EP1557222A3 (fr) 2007-05-09
US7434484B2 (en) 2008-10-14
DE102004003433B4 (de) 2006-03-23
DE102004003433A1 (de) 2005-08-25
US20050155439A1 (en) 2005-07-21
EP2263800A3 (fr) 2014-05-21
EP2263800A2 (fr) 2010-12-22

Similar Documents

Publication Publication Date Title
EP1557222B1 (fr) Pipette avec éjecteur d'embouts de pipette
EP1559480B1 (fr) Pipette avec mécanisme de déplacement fixé de façon détachable au mécanisme d'actionnement
EP1598113B1 (fr) Pipette
EP2633913B1 (fr) Dispositif de pipetage et dispositif de pipetage multi-canal
EP0062206B1 (fr) Dispositif pneumatique-hydraulique pour la pose de rivets aveugles
EP1084798B1 (fr) Outil de pressage hydraulique
EP2735369B1 (fr) Pipette multi-canal
WO2018002254A1 (fr) Tête de dosage, dispositif de dosage comprenant une tête de dosage et procédé de dosage au moyen d'une tête de dosage
EP3680016B1 (fr) Pipette destinée à une utilisation avec une pointe de pipette
DE102005041183B3 (de) Pipettiervorrichtung
DE102006031460B4 (de) Pipettiervorrichtung
DE102007010299A1 (de) Handpipettiervorrichtung
DE102005023203A1 (de) Pipette
EP0992288A2 (fr) Mechanique d'éjection d'embouts de pipette
DE69636419T2 (de) Schraubenführungsvorrichtung einer Vorrichtung zum Setzen und Eindrehen von Schrauben
EP3793738A1 (fr) Tête de pipetage, dispositif de pipetage comprenant une tête de pipetage et procédé de pipetage au moyen d'une tête de pipetage
EP3680017B1 (fr) Pipette destinée à une utilisation avec une pointe de pipette
EP2659978B1 (fr) Pipette avec systeme de blockage
DE19708151C2 (de) Pipettiervorrichtung
EP2626134B1 (fr) Pipette
EP2210667B1 (fr) Dispositif de dosage avec éjecteur de pointe
DE10355914B3 (de) Pipettiervorrichtung mit einer Abwurfeinrichtung für Pipettenspitzen
DE4127269C2 (de) Pneumatisches Schraubgerät
DE102004055562B4 (de) Handhabbares Dosiersystem
DE102022105534A1 (de) Luftpolsterpipette

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

AK Designated contracting states

Kind code of ref document: A2

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU MC NL PL PT RO SE SI SK TR

AX Request for extension of the european patent

Extension state: AL BA HR LV MK YU

PUAL Search report despatched

Free format text: ORIGINAL CODE: 0009013

AK Designated contracting states

Kind code of ref document: A3

Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HU IE IS IT LI LT LU MC NL PL PT RO SE SI SK TR

AX Request for extension of the european patent

Extension state: AL BA HR LV MK YU

17P Request for examination filed

Effective date: 20070420

17Q First examination report despatched

Effective date: 20070706

AKX Designation fees paid

Designated state(s): DE FR GB IT

GRAP Despatch of communication of intention to grant a patent

Free format text: ORIGINAL CODE: EPIDOSNIGR1

INTG Intention to grant announced

Effective date: 20140623

GRAS Grant fee paid

Free format text: ORIGINAL CODE: EPIDOSNIGR3

GRAA (expected) grant

Free format text: ORIGINAL CODE: 0009210

AK Designated contracting states

Kind code of ref document: B1

Designated state(s): DE FR GB IT

REG Reference to a national code

Ref country code: GB

Ref legal event code: FG4D

Free format text: NOT ENGLISH

REG Reference to a national code

Ref country code: DE

Ref legal event code: R096

Ref document number: 502005014586

Country of ref document: DE

Effective date: 20141224

REG Reference to a national code

Ref country code: DE

Ref legal event code: R097

Ref document number: 502005014586

Country of ref document: DE

PLBE No opposition filed within time limit

Free format text: ORIGINAL CODE: 0009261

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: NO OPPOSITION FILED WITHIN TIME LIMIT

26N No opposition filed

Effective date: 20150813

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 12

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 13

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 14

REG Reference to a national code

Ref country code: FR

Ref legal event code: PLFP

Year of fee payment: 19

REG Reference to a national code

Ref country code: DE

Ref legal event code: R081

Ref document number: 502005014586

Country of ref document: DE

Owner name: EPPENDORF SE, DE

Free format text: FORMER OWNER: EPPENDORF AG, 22339 HAMBURG, DE

P01 Opt-out of the competence of the unified patent court (upc) registered

Effective date: 20230527

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: DE

Payment date: 20240119

Year of fee payment: 20

Ref country code: GB

Payment date: 20240123

Year of fee payment: 20

PGFP Annual fee paid to national office [announced via postgrant information from national office to epo]

Ref country code: IT

Payment date: 20240122

Year of fee payment: 20

Ref country code: FR

Payment date: 20240124

Year of fee payment: 20