EP2626134B1 - Pipette - Google Patents

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Publication number
EP2626134B1
EP2626134B1 EP13000386.6A EP13000386A EP2626134B1 EP 2626134 B1 EP2626134 B1 EP 2626134B1 EP 13000386 A EP13000386 A EP 13000386A EP 2626134 B1 EP2626134 B1 EP 2626134B1
Authority
EP
European Patent Office
Prior art keywords
housing part
ejection
decoupling
stroke
pipette
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP13000386.6A
Other languages
German (de)
English (en)
Other versions
EP2626134A2 (fr
EP2626134A3 (fr
Inventor
Holger Link
Jens Wilmer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Priority to PL13000386T priority Critical patent/PL2626134T3/pl
Publication of EP2626134A2 publication Critical patent/EP2626134A2/fr
Publication of EP2626134A3 publication Critical patent/EP2626134A3/fr
Application granted granted Critical
Publication of EP2626134B1 publication Critical patent/EP2626134B1/fr
Active legal-status Critical Current
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/023—Adapting objects or devices to another adapted for different sizes of tubes, tips or container
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/028—Modular arrangements

Definitions

  • the invention relates to a pipette with a discharge device for pipette tips.
  • Pipettes are used especially in the laboratory for dosing liquids.
  • a pipette tip is clamped with an upper opening on a neck of the pipette. Through a lower opening, the pipette tip can absorb and dispense liquid.
  • Bubble pipettes include a positive displacement device for air communicating with the pipette tip through the hub. By means of the displacement device, an air cushion is displaced, so that liquid is sucked into the pipette tip and ejected therefrom.
  • the displacement device is usually a cylinder with a piston displaceable therein.
  • the pipette tips are released from the batch after use and replaced with a fresh pipette tip. As a result, contamination can be avoided at subsequent dosages.
  • Single-use pipette tips are available inexpensively from plastic.
  • the approach for releasably holding pipette tips is a conical or cylindrical projection with respect to a housing of the pipette, on which a pipette tip with a matching upper opening is clamped. This can be done without touching the pipette tip by pressing the pipette with the approach in the upper opening of the pipette tip standing in a holder.
  • known pipettes have a discharge device with a drive device. By actuating a ejector button, a discharge slide is displaced via a discharge rod, that he loosens the pipette tip from the approach without the user having to touch it.
  • These pipettes have a dosing button for operating the displacement device and the separate ejector button for actuating the ejection device, so that two buttons must be operated.
  • a hand-held pipette with one-button operation known.
  • This has a housing from which protrudes above a depressible against spring action actuator, which is connected via a lifting rod with a piston movable in a cylinder.
  • the piston Upon actuation of the actuator, the piston performs an adjustable power stroke.
  • the piston can perform an overstroke.
  • there is a separable magnet arrangement between the actuating element and piston-cylinder unit which indicates to the user the end of the working stroke through a resistance, after which the overstroke is carried out.
  • a discharge stroke is provided for ejecting the pipette tip.
  • the piston rod has a stop element, which can move a discharge sleeve at an actuating end, so that it pushes the pipette tip at its upper edge from the approach with a discharge end.
  • the beginning of the discharge stroke is indicated to the user by the resistance of a spring acting on the discharge sleeve.
  • the piston is moved, so that a clearance in the cylinder is needed.
  • the free space forms a dead volume, which affects the dosing accuracy.
  • EP 0 181 957 A1 describes a pipette that extends a Dosierhub when by pressing a pushbutton a piston rod. is moved down until a Anschlagstcin meets a magnetic plate. For a Ausblashub the push button is further depressed, so that the magnetic plate from a housing-fixed Ferromagnetplättchen dissolves and hits a moving with the magnetic plate down sleeve on a ring at the top of a thrower. For ejecting the pipette tip, the push button is pressed even deeper, so that the spacer sleeve over the ring the ejector down displaced and the ejector sleeve, the pipette tip from a neck off. This pipette also has a large dead volume. In addition, the ejection of a pipette tips requires a great deal of effort, because a piston return spring, a blowout spring and a Abwerferfeder must be compressed.
  • the DE 197 08 151 A1 describes another pipette with one-button operation, which has a lower dead volume.
  • the pipette has a moving means, a piston for moving air movable therefrom in a cylinder, a mounting boss having a connection with the cylinder-having opening for fixing a pipette tip, and a movement of the piston when moving air to the opening of the attachment approach limiting stop.
  • the stop is an end face of the cylinder, which the piston encounters when moving air to the opening of the attachment approach.
  • the pipette has a discharge device, which has a discharge section for separating a pipette tip from the attachment approach during a dropping movement of the discharge device and an actuation section for effecting the discharge movement.
  • a decoupling device which decouples the piston when reaching the stop of the movement device.
  • the decoupling device is formed by a magnet arrangement with a magnet and a magnetizable counterpart, which come loose after overcoming a holding force.
  • a driver coupled to the movement device acts on the actuation section when the piston is decoupled, wherein movement of the carrier by the movement device forces the ejection movement of the ejection device.
  • the movement of the piston is limited by the stroke after the power stroke and possibly a Ausblashub to blow out residual fluid. Then the decoupling device decouples the piston from the moving means.
  • the piston Upon further actuation of the movement means in the same sense, the piston is not moved further, however, a coupled with the moving means driver moves, which acts on the actuating portion of the ejection device and forces the ejection movement.
  • the ejection movement without further movement of the piston reduces the dead volume and improves the dosing accuracy.
  • the piston In the known pipette, the piston is loaded when hitting the end face of the cylinder, so that it must be carried out sufficiently stable. For small sizes for low dosing volumes, this is expensive.
  • the upper part of the housing which includes the decoupling device and the piston, must be with a housing base are screwed together, which includes the cylinder.
  • the housing tops In the production of pipettes with different sizes for different Dosier Symposiume the housing tops must be equipped with different decoupling devices and cylinders and then assembled with the respective housing lower parts.
  • the DE 10 2004 003 434 B4 describes a pipette with a displacement device and a drive device releasably connected thereto.
  • the displacement device has a displacement chamber with a displaceable restriction, a connection for connection to a pipette tip and a connection channel between the displacement chamber and the free end of the attachment.
  • the drive device for driving the displaceable boundary of the displacement device has a drive element which has a detachable operative connection with the displaceable boundary. Between the drive device and the displacement device, a bayonet connection is present, which can be produced producing the operative connection between the drive element and displaceable boundary and releasing the active connection between the drive element and displaceable boundary.
  • the pipette has a separate ejection device for releasing a pipette tip from the projection, which has a release drive arranged separately on the drive device, an ejector arranged on the displacement device and a detachable axial clamping connection directed between the discharge drive and ejector in the direction of the longitudinal axis of the bayonet connection.
  • the object of the invention is to provide a pipette which reduces the effort for the provision of different sizes and is easier to handle.
  • the pipette according to the invention has a displacement unit and a drive unit, which are detachably connected to each other by means of a detachable connection.
  • the detachable connection can be configured as a bayonet connection, as in the DE 10 2004 003 434 B4 described.
  • the displacement unit is arranged in a housing lower part. It includes the displacement chamber and the movable boundary. This is preferably
  • a cylinder with a piston displaceably arranged therein When assembling the displacement unit and drive unit so the piston or another movable limit must not be installed in the displacement unit.
  • the drive element When the upper housing part is connected to the lower housing part is, the drive element is applied to the contact element.
  • the coupling of the drive device of the drive unit with the displaceable boundary is effected in that the drive element of the drive unit contacts the contact element of the displacement unit.
  • the contact can be easily made by assembling and making the detachable connection between the displacement unit and the drive unit.
  • the pipette has an end stop on which an end stop element connected to the drive element strikes, in order to limit the displacement of the drive element downwards.
  • the end stop is not the bottom face of the cylinder. Rather, the end stop is arranged in the upper housing part and fixedly connected to the upper housing part and the drive element has the end stop element. As a result, the piston is not subjected to stress by hitting the end stop.
  • the end stop and end stop element can be designed so that they withstand the largest forces expected. It is also possible to use displacement units that are not designed so that the piston hits the end face of the cylinder at the end of the displacement downwards.
  • the piston Upon reaching the end stop, the piston is preferably just before or just on the lower end face of the cylinder in order to keep the dead volume and associated dosing errors as low as possible.
  • the drive unit has a decoupling device between the drive element and drive member. The decoupling device decoupled at system of the end stop element on the end stop the drive element of the drive device when the actuating element is further displaced in the direction of actuation. Consequently the drive device is moved on in the same sense as before.
  • the drive device is coupled to a lower stop body, which strikes the operating end of the discharge extension when the drive element is decoupled from the drive device.
  • the pipette according to the invention thus enables the control of the dosage and the ejection of pipette tips by operating only a single actuator.
  • the displacement chamber and displaceable boundary are not adversely affected. Larger dead volumes and associated metering errors are avoided.
  • the basic structure of the pipette of displacement unit and drive unit facilitates assembly and disassembly.
  • the displacement unit and drive unit can be used to assemble different types of pipettes.
  • displacement units which are not originally designed to perform the control of dosing operations and discharge operations by means of only one actuator, may be used with the drive unit to form one-touch operation pipettes.
  • the same drive unit can be assembled with different displacement units to form pipettes with different dosing ranges.
  • the actuating element interacts with the drive device by being mechanically or directly connected therewith.
  • the drive element cooperates with the drive device, in which it is directly or indirectly mechanically connected thereto.
  • the actuating direction of the actuating element preferably runs in a straight line. It preferably runs in the longitudinal direction of the rod-shaped housing. Alternatively, it runs in one Angle (for example, 45 ° or 90 °) to. According to another alternative, the actuating direction runs along a curved curve, for example on a circular path. In a rectilinear actuator, the actuator is preferably a push button and in a curved actuator a pivoting lever.
  • the displacement unit is according to a preferred embodiment, a cylinder with a piston displaceable therein.
  • the displacement unit is a displacement chamber with a flexible wall or membrane displaceable therein.
  • the drive device is according to a preferred embodiment, a lifting rod.
  • the lifting rod is a cylindrical lifting rod.
  • the lifting rod is made of metal or plastic.
  • the contact element is according to a preferred embodiment, a piston plate at the upper end of the piston or a piston rod connected to the upper end of the piston.
  • the piston plate is preferably aligned horizontally.
  • the contact element is the upper end of the piston or the upper end of a piston rod connected to the piston.
  • the lower stopper body is according to a preferred embodiment, against the action of an overstroke spring downwardly displaceable stop body on which a stop element of the drive means impinges at the end of a Dosierhubes.
  • an overstroke is performed and displaced the stopper body against the action of the over-travel spring down.
  • the pipette is a hand-held pipette, ie a pipette, which the user can hold and operate during pipetting with just one hand.
  • the drive device has a stop element and an upper stop body and the lower stop body, between which the stop element is displaceable to perform a Dosierhub, wherein the lower stop body is supported via a Kochhubfeder in the upper housing part to after execution of the metering by further Relocating the actuating element in the direction of actuation against the spring force of the overstroke spring to reach the end stop by the end stop an overstroke.
  • the stopper member is moved between the upper and lower stopper body, being displaced from the upper stopper body to the lower stopper body for sucking liquid in a pipette tip attached to the approach from the lower stopper body to the upper stopper body and to eject air or liquid from the pipette tip.
  • the impact of the stop element feels the user, since the lower stop body is supported by a Kochhubfeder in the upper housing part. As a result, it is ensured when taking in liquid that exactly the intended metering stroke is performed.
  • the user can also perform an overstroke to blow out residual liquid from the pipette tip.
  • the user can overcome the spring force of the overstroke spring and cause an overstroke until the drive element is stopped by the end stop.
  • the user can control the ejection of the pipette tip in the manner described by decoupling the decoupling device by applying a corresponding force to the actuator.
  • the lower stop body sits with its outer edge at the end of the overstroke or at the beginning of the further displacement of the actuating element in the direction of actuation on the operating end of the ejection extension to move as a driver, the ejection extension down.
  • the lower stopper body satisfies the functions to limit the metering stroke, to allow the overtravel and to control the ejection of the pipette tip in an advantageous manner that reduces the structural complexity.
  • this embodiment makes it possible for the discharge extension to be guided laterally past the overstrokes, the drive element and the decoupling device.
  • space for an ejection spring for resetting the ejection device is thereby created below the overstroke to a starting position in which a pipette tip is attachable to the approach.
  • the upper stop body in the upper housing part is displaceable and has the upper housing part coupled to the upper stop body adjusting device for adjusting the position of the upper stop body and set the Dosierhubes on.
  • the amount of liquid to be metered is adjustable.
  • the upper stop body is according to another embodiment, a threaded spindle having an upper passageway through which a lifting rod of the drive means is passed.
  • the threaded spindle is arranged by means of the adjusting in a housing upper part and fixedly connected to this spindle nut rotatable and thereby axially displaceable.
  • the lower end face of the threaded spindle serves as a stop for the stop element. The position of the lower end face is decisive for the metering stroke.
  • the stop element is a collar on the circumference of the lifting rod and the lifting rod is hin malerstreckt through an upper passageway of the upper stop body and through a lower passage channel of the lower stop body and the collar between the upper stop body and the lower stop body is arranged.
  • the decoupling device comprises two decoupling elements, of which one decoupling element is connected to the drive device and the other decoupling element to the drive element, wherein the decoupling elements are releasably held together in a certain connection position and after removing a certain holding force from each other and removable the holding position are displaced back.
  • the decoupling device can be easily integrated between the drive device and the drive element. The decoupling device decouples only when the force acting on the decoupling device by actuation of the actuating element exceeds the holding force. The user thus senses a resistance at the end of dispensing liquid from a pipette tip.
  • the holding force is preferably a maximum of 25N, more preferably a maximum of 22N.
  • the holding force is preferably at least 10N, furthermore preferably at least 16N. It is preferably 19N.
  • the decoupling elements comprise two magnets or a magnet and a ferromagnetic counterpart and / or a pawl device and / or a spring device which the Releasably hold the decoupling elements in the connection position.
  • the decoupling elements preferably have a magnet arrangement with two magnets or a magnet and a ferromagnetic counterpart.
  • the magnet is preferably a permanent magnet.
  • the decoupling device can in particular be designed as in the DE 197 08 151 A described.
  • the drive element is a sleeve whose lower end rests on the contact element
  • the decoupling device comprises an upper decoupling element with an upper through hole, which is arranged in the sleeve and fixedly connected thereto, and a lower decoupling element which is displaceable in the Sleeve is arranged and fixed to the lower end of a lifting rod, which is from the top into the sleeve and through the upper through hole extends therethrough, and the sleeve through a further through hole of the end stop extends and has a radially outwardly projecting further collar, the the movement of the drive element is limited downwards by seating on top of the end stop.
  • the decoupling device is integrated to save space in the drive element.
  • the decoupling device can be mounted with the drive element on the lifting rod of the drive device, the end stop element is easily realized by a further collar of the sleeve.
  • a decoupling spring is arranged in the sleeve below the lower decoupling element, via which the decoupling element is supported in the sleeve.
  • the decoupling spring presses to relieve the Actuator the lower decoupling element to the upper decoupling element back to bring the decoupling element in the connecting position.
  • the sleeve has a lower sleeve portion and an upper sleeve portion, wherein the lower sleeve portion has a smaller diameter than the upper sleeve portion is hineinerstreckt through the upper housing opening of the housing base and seated with the lower end on the contact element and in the upper sleeve portion the decoupling elements are arranged.
  • the decoupling elements are arranged.
  • the overstroke spring is supported on an annular Göhubfederlager in the upper housing part, the sleeve passed through the through hole of the Studentshubfederlagers and is the overstroke spring bearing the end stop for the collar of the sleeve.
  • the function of the overstroke spring bearing and the end stop is merged and the structural complexity is reduced.
  • the discharge extension is supported in the upper housing part by a discharge spring arranged below the overhub spring bearing in the upper housing part.
  • the discharge spring is at the top of a arranged below the Matterhubfederlagers upper Abschfederlager to the Shedding extension and supported at the bottom of a housing fixed lower Abschfederlager.
  • the discharge slide is a discharge sleeve, which is guided on its inner circumference on the housing lower part.
  • the discharge sleeve has the shape of a sleeve.
  • the Abschverinrung a plurality of parallel rods, which are connected to each other at a distance from the discharge slide by an annular upper Abschfederlager.
  • the execution of the discharge slide as a sleeve allows easy installation on the displacement unit.
  • the discharge slide for cleaning, maintenance or repair is easy to disassemble and assemble.
  • the design of the ejection extension with several parallel rods favors a space-saving guidance in the housing upper part. Due to the annular ejection spring bearings, the rods are stabilized in the upper region and prevented from accidentally pulling out of the upper housing part.
  • the discharge slide and the discharge extension are connected to each other by at least one snap connection.
  • a snap connection facilitates the mounting and, if necessary, dismantling of discharge slide and discharge extension.
  • the discharge extension is connected by a first snap connection with a Abschring and the Abschring is connected by a second snap connection with the discharge slide.
  • the overstroke spring and / or the decoupling spring and / or the piston spring and / or the ejection spring is / are a helical spring and / or a control spring.
  • Coil springs or conical springs are advantageously accommodated in the rod-shaped housing and can be provided with a suitable spring hardness.
  • the terms “up” and “down”, “above” and “below” and “horizontally” and “vertically” refer to an orientation of the pipette in which the housing is aligned with the approach vertically downwards. In this orientation, an attached on the approach pipette tip can be directed to an underlying vessel to absorb liquid or give.
  • the pipette 1 has a rod-shaped, molded as a handle housing with an upper housing part 2 and a lower housing part 3.
  • the upper housing part 2 with all the parts included forms a drive unit and the lower housing part 3 with all parts contained a displacement unit.
  • an actuator 4 in the form of a cylindrical push button out.
  • the actuating element 4 is mounted axially displaceably and rotatably in the upper housing part 2.
  • the actuating element 4 is screwed with a thread in a cover 5 on a cylindrical lifting rod 6.
  • the lifting rod 6 is passed in the upper housing part 2 through an upper passage channel 7 of a threaded spindle 8.
  • the threaded spindle 8 is screwed into an internal thread of a housing upper part 2 held in a defined position spindle nut 9.
  • the spindle nut 9 is fixedly connected to a lifting body 10 which is fixed in the upper housing part 2.
  • the lifting body 10 is substantially cylindrical and is a support for the spindle nut 9, the threaded spindle 8 screwed therein and the lifting rod 6 guided therein.
  • these and other components are preassembled on the lifting body 10 and the lifting body 10 equipped with the components is mounted in the upper housing part 2, so that it is firmly held in the upper housing part 2.
  • the lifting body 10 is locked to the upper housing part 2.
  • the upper housing part 2 can be equipped inside corresponding to the lifting body 10.
  • the threaded spindle 8 has a rotatably connected thereto Spindelmitcret 11 above.
  • the Spindelmitstream 11 has on the circumference a hexagon 12 with a central hole 13.
  • the hexagon 12 engages in a Innsensechskant 14 of the actuating element 4 a.
  • the actuator 4 is provided at the bottom with two diametrically opposed, radial projections 15, 16 which project outwardly.
  • radial projections 15, 16 are present.
  • the radial projections 15, 16 engage in axially extending grooves 17, 18 on the inside of a hollow cylindrical transmission part 19, which is rotatably mounted in the upper housing part 2.
  • the transmission member 19 has at the top of the outer circumference a sprocket 20 which meshes with a gear of a counter gear 21 which drives a plurality of juxtaposed on a horizontal axis counter wheels 22 of a counter 23.
  • the counter 23 is attached to the upper housing part.
  • the Zählwerks impart 22 each have digits from 0 to 9. Das - based on Fig. 1 - The last counter gear 22 is driven by the counter gear 21. The next one arranged counting wheels 22 are further rotated in each case by one digit when the behind arranged Zählwerksrad passes from 9 to 0.
  • the upper housing part 2 has a housing cover 24 with a window through which the digits of the counter wheels 22 can be read.
  • a beaded collar 25 is arranged as a stop element on the lifting rod 6. The displacement of the lifting rod 6 upwards is limited by contact of the collar 25 at the lower end face 26 of the threaded spindle 8, which forms an upper stop body for the collar 25.
  • a substantially disc-shaped lower stop body 27 is arranged in the lifting body 10.
  • the lower stop body 27 has a cup-shaped depression, in which a central passageway 28 is arranged centrally. Further, the lower stopper body 27 has three radially outwardly projecting projections 29 which are distributed uniformly around its circumference.
  • the lower stop member 27 is guided on the projections 29 in axially extending guide slots 30 of the lifting body 10. This is also in Fig. 3 shown. Upwards he is 30 until the projection of the projections 29 at the upper end of the guide slots 30 displaced.
  • a lifting spring 31 designed as a helical spring is arranged in the lifting body 10.
  • the overstroke spring bears against the lower side of the lower stop body 27.
  • Bottom supports the overtravel spring 31 abuts against an overhub spring bearing 32 which is arranged and thus firmly connected.
  • the over-stroke spring bearing 32 is formed by a ring with L-section, wherein the horizontal leg of the L-profile surrounds a central through hole 33 of the overstroke spring bearing 32.
  • the overstroke spring 31 is supported on the horizontal leg of the L-profile and is laterally bordered by the vertical leg. The overstroke spring 31 presses the lower stopper body 27 with the protrusions 29 under bias against the upper ends of the guide slots 30.
  • a drive element 34 in the form of a coaxial with the lifting rod 6 aligned sleeve is present in the lifting body 10.
  • the drive member 34 has an upper sleeve portion 35 and a lower sleeve portion 36, wherein the upper sleeve portion 35 has larger inner and outer diameter than the lower sleeve portion 36.
  • the lower sleeve portion 36 has a frusto-conical tip 37 at the bottom.
  • a radially outwardly projecting, further circumferential collar 38 is present at the upper edge of the upper sleeve portion 35.
  • the outer diameter of the upper sleeve portion 35 is smaller than the inner diameter of the through hole 33 of the overstroke spring bearing 32, so that the lower and the upper sleeve portion 35, 36 are insertable into the through hole 33.
  • the outer diameter of the further collar 38 exceeds the inner diameter of the through-hole 33, so that the drive element 34 can not completely pass through the through-hole 33.
  • the over-stroke spring bearing 32 forms an end stop and the further collar 38 an end stop element, which limit the displacement of the drive element 34 down.
  • a hollow cylindrical armature 39 is arranged from a ferromagnetic material above. Below this, a hollow cylindrical magnet 40 is arranged in the upper sleeve section 35. Below this is a pot 41 which receives the magnet 40.
  • the armature 39 has an interference fit in the upper sleeve portion 35.
  • the lifting rod 6 extends slidably through the central hole of the armature 39 therethrough. Below the armature, the lifting rod 6 has a needle-shaped portion 42 of reduced diameter.
  • the magnet 40 and the pot 41 are seated on the needle-shaped portion 42.
  • the magnet 40 and cup 41 are fixed on the needle-shaped portion 44, e.g. by having a press fit there.
  • the magnet 40 is supported at the top on a shoulder of the lifting rod from which the needle-shaped portion 42 extends.
  • FIG. 1 . 2 . 5 and 6 is the upper housing part 2 below next to a lower housing opening 45 on the inner circumference with unspecified means for releasably connecting 46 with further means for releasably connecting the housing base 3 is provided.
  • the lower housing part 3 has a hollow cylindrical section 47 at the top, followed by a short upper hollow cone section 48 with a large cone angle, followed in turn by a long lower hollow cone section 49 with a small cone angle, with its lower end a conical projection 50 for clamping a pipette tip 51 forms.
  • a clamped pipette tip 51 is also substantially conical with an upper opening 52 for attachment to the lug 50 and a lower opening 53 for the passage of liquid.
  • the upper opening 52 is substantially larger than the lower opening 53 and from the upper to the lower opening the pipette tip tapers 51
  • the hollow cylindrical portion 47 of the lower housing part 3 is provided at the top of the outer circumference with other means for releasable connection 54, which are not described in more detail, which are adapted to the means for releasably connecting 46 of the upper housing part 2 to releasably connect the lower housing part 3 with the upper housing part 2.
  • Suitable means for releasably connecting 46, 54 of the upper housing part 2 and lower housing part 3 are in the DE 10 2004 003 434 B4 described.
  • the lower hollow cone section 49 has at the top a projection 55 extending beyond the upper floc cone section 48 in the lower housing part 3.
  • the lower hollow cone portion 49 has a connecting channel 56 which connects the upper end face of the extension 55 with the lower end face of the projection 50.
  • an arrangement of a cylinder 57 is arranged with a displaceable piston 58 therein.
  • the cylinder 57 is inserted with a lower portion in the connecting channel 56 and fixed therein by press-fitting or gluing.
  • the cylinder 57 is sealed off from the connecting channel 56 by means of an O-ring 59.
  • the piston 58 has on the circumference a piston seal 60, which seals on the inside of the cylinder 57. Below the piston seal 60, the piston 58 has a needle-shaped Extension 61, which is insertable into a through hole 62 in the bottom of the cylinder 57 and in the connecting channel 56 in order to reduce the dead volume. Cylinder 57 and piston 58 are vertically aligned. At the top, the piston 58 carries a horizontally oriented piston plate 63, which has at the center a vertically oriented, conical recess 64 for receiving the tip 37 of the drive member 34.
  • the lower housing part 3 has at the top a pot-shaped closure cap 65 with a cylindrical or conical jacket.
  • the bottom of the cap 65 is disposed above the piston plate 63 and has a central upper housing opening 67, through which the piston plate 63 is accessible from above.
  • the cap 65 has outwardly projecting protrusions 68 which are snapped into corresponding recesses 69 of the hollow cylindrical portion 47 of the housing base 3.
  • the bottom of the cap 65 limits the displacement of the piston 58 upwards.
  • a trained as a spiral spring piston spring 70 which is supported at the bottom of the extension 55 and the top of the underside of the piston plate 63, biases the piston 58 against the underside of the cap 65 before.
  • the pipette 1 has a discharge device 71.
  • the discharge device 71 comprises a discharge slide 72, which is arranged on the housing lower part 3.
  • the discharge slide 72 has a contour adapted to the contours of the hollow cylindrical section 47, the upper hollow cone section 48 and the lower hollow taper section 49.
  • Below it has an annular discharge end 73.
  • the discharge end 73 is in the position of the discharge slide 72 of Fig. 1 pushed as far as possible up to the lower housing part 3, so that the conical projection 50 is free for attaching a pipette tip 51.
  • a discharge extension 74 This includes three vertical discharge bars 75, which are connected to the upper edge of the discharge slide 72.
  • the discharge bars 75 are evenly distributed over the upper edge of the discharge slide 72.
  • the discharge bars 75 are connected at the bottom via a first snap connection with a discharge ring 76, which is connected via a second snap connection with the upper edge of the discharge slide 72.
  • the discharge rods 75 are connected at the top at its inner periphery by an annular upper Abschfederlager 77 with each other.
  • the upper Abschfederlager 77 has an L-shaped cross section, wherein the horizontal leg of the cross section, the through hole 33 adjacent to the Matterhubfederlagers 32.
  • the vertical, circumferential leg of the upper Abschfederlagers 77 is directed downward.
  • the discharge bars 75 have actuation ends 78 at the top.
  • the discharge extension 74 and the discharge bars 75 are hineinerstreckt through the lower housing opening 45 in the upper housing part 2.
  • the displacement of the Abschverinrung 74 upwards is limited by conditioning the upper Abschfederlagers 77 on the underside of the Kochhubfederlagers 32.
  • the webs 79 form a lower Abschfederlager 80.
  • a designed as a helical spring ejection spring 81 is under bias between the upper Abschfederlager 77 and the lower Abschfederlager 80th arranged and pushes the ejector 71 upwards, so that the upper Abschfederlager 77 abuts the overstroke spring bearing 32.
  • the lower housing part 3 is guided with an upper region of the hollow cylindrical section 47 into the lower housing opening 45 of the housing upper part 2.
  • the means for releasably connecting 46, 54 of the lower housing part 3 and upper housing part 2 are detachably connected to each other.
  • the drive member 34 engages with the tip 37 in the upper housing opening 67 and is located in the recess 64 on the piston head 63 at.
  • the piston plate 63 pushes the drive member 34 upwards and via the decoupling device 44, the lifting rod 6 is pressed against the threaded spindle 8 with the collar.
  • a pipette tip 51 is clamped on the lug 50, preferably by being pressed into a pipette tip 51, which is held in a holder.
  • Remains remaining in the pipette tip 51 can be blown out by pressing the actuating element 4 further down under increased force becomes.
  • the lower stopper body 27 is displaced against the action of the overstroke spring 31 in the guide slots 30 down and on the decoupling device 44, the drive element 34 further displaced downwards, which pushes the piston 58 even deeper into the cylinder 57.
  • a further overpressure is generated which pushes out residual liquid adhering to the inner wall of the pipette tip 51.
  • the overstroke is completed when the drive element 34 with the other collar 38 strikes the overstroke spring bearing 32, which forms the end stop.
  • the projections 29 reach the actuating end 78 of the discharge extension 74 or are located at a very small distance above it. This situation is in Fig. 7c shown.
  • the pipette tip 51 can be dropped.
  • the actuator 4 is pressed with increased force further down.
  • the over-stroke spring bearing 32 prevents the drive member 34 from moving further down.
  • the magnet 40 releases from the armature 39 and the lifting rod 6 moves further down and takes the lower stopper body 27 with.
  • the lower stopper body 27 presses with the projections 29, the discharge extension 74 to the operating ends 78 down.
  • the Abschverinrung 74 takes the discharge slide 72 with downward, which pushes the pipette tip 51 from the approach 50 with the discharge end 73.
  • the pipette 1 After releasing the actuator 4, the pipette 1 automatically returns to the initial situation of Fig. 1 . 2 . 5 . 6 and 7a back.
  • the ejection spring 81 pushes the discharge extension 74 and thus the discharge slide 72 upwards.
  • the decoupling spring 43 pushes the lifting rod 6 with the magnet 40 upwards until the magnet 40 is held by the magnetic forces on the armature 39.
  • the overstroke spring 31 pushes the lower stopper body 27 upward until the projections 29 have arrived at the upper ends of the guide slots 30.
  • the piston spring pushes the piston 58, the drive element 34 and the decoupling device 44, the lifting rod 6 upwards until the collar 25 rests against the threaded spindle 8.
  • the upper housing part 2 can be easily connected to another lower housing part 3, in which the cylinder 57 and the piston 58 have a different cross-section. Accordingly, the same upper housing part 2 can be used for the production of pipettes 1 with different dosing volumes. Furthermore, it is possible, in the event of a defect, to easily replace the lower housing part 3 or upper housing part 2 or, if required, to fit the upper housing part 2 with another lower housing part 3. In addition, the housing lower part 3 can be easily removed from the upper housing part 2 for maintenance, repair and cleaning purposes.
  • actuation force to be applied increases only slightly when the metering stroke is carried out.
  • the Dosierhub is finished after about 14mm actuation.
  • the force to be exerted on the actuating element 4 must first be greatly increased, from about 3 Newton to about 10.5 Newton. Thereafter, an increasing operating force is applied for the overstroke, which increases slightly more than the actuation force for carrying out a Dosierhubes. The overtravel is completed after about 17mm actuation.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Quick-Acting Or Multi-Walled Pipe Joints (AREA)
  • Sampling And Sample Adjustment (AREA)

Claims (15)

  1. Pipette, avec
    - une unité de refoulement comprenant une partie inférieure de boîtier (3) en forme de tige avec une chambre de refoulement qui y est disposée intérieurement avec une limitation déplaçable, avec un élément de contact raccordé à la limitation déplaçable sur un orifice de boîtier (67) supérieur en haut sur la partie inférieure de boîtier (3), avec un embout (50) pour le raccordement à une pointe de pipette (51) en bas sur la partie inférieure de boîtier (3) et avec un canal de raccordement (56) entre la chambre de refoulement et l'extrémité libre de l'embout (50),
    - une unité d'entraînement pour l'entraînement de la limitation déplaçable de l'unité de refoulement dans une partie supérieure de boîtier (2) en forme de tige, avec un système d'entraînement (6) qui y est disposé intérieurement, avec un élément d'actionnement (4), coopérant avec le système d'entraînement, sur la partie supérieure de boîtier (2) et avec un élément d'entraînement (34) qui coopère avec le système d'entraînement sur un orifice de boîtier (45) inférieur en bas sur la partie supérieure de boîtier (2) qui peut être déplacé vers le bas par l'actionnement de l'élément d'actionnement (4) dans la direction d'actionnement pour réaliser une course de dosage de la limitation déplaçable,
    - des moyens de raccordement détachable (46, 54) qui raccordent l'une à l'autre de façon détachable la partie supérieure de boîtier (2) et la partie inférieure de boîtier (3) dans un agencement dans lequel l'élément d'entraînement (34) est adjacent à l'élément de contact, pour former un boîtier en forme de tige,
    - un système de délivrance (71) qui présente un curseur de délivrance (72) disposé de façon déplaçable sur la partie inférieure de boîtier (3) qui a en bas une extrémité de délivrance (73) pour le desserrage d'une pointe de pipette (51) par rapport à l'embout (50), et le système de délivrance (71) présente également un prolongement de délivrance (74), disposé de façon déplaçable sur la partie supérieure de boîtier (2), qui est raccordé en bas à l'extrémité supérieure du curseur de délivrance (72) et qui a en haut une extrémité d'actionnement (78),
    - une butée d'extrémité (32) disposée dans la partie supérieure de boîtier (2), raccordée de façon fixe à la partie supérieure de boîtier (2), et un élément de butée d'extrémité (38) raccordé à l'élément d'entraînement (34) pour la limitation du déplacement de l'élément d'entraînement (34) vers le bas,
    - un système de découplage (44) entre le système d'entraînement et l'élément d'entraînement (34) qui, lors de l'appui de l'élément de butée d'extrémité (38) contre la butée d'extrémité (32) et lors de la poursuite du déplacement de l'élément d'actionnement (4) dans la direction d'actionnement, découple l'élément d'entraînement (34) par rapport au système d'entraînement, et
    - un corps de butée (27) inférieur pouvant être couplé au système d'entraînement qui, lors du découplage de l'élément d'entraînement (34) par rapport au système d'entraînement par le système de découplage (44), heurte l'extrémité d'actionnement (78) du prolongement de délivrance (74) pour déplacer vers le bas le prolongement de délivrance (74) et le curseur de délivrance (72) lors de la poursuite du déplacement de l'élément d'actionnement (4) dans la direction d'actionnement.
  2. Pipette selon la revendication 1, dans laquelle le système d'entraînement présente un élément de butée (25) sur une tige de course du système d'entraînement, un corps de butée (8) supérieur et le corps de butée (27) inférieur entre lesquels l'élément de butée (25) peut être déplacé pour réaliser une course de dosage, le corps de butée (27) inférieur étant appuyé dans la partie supérieure de boîtier (2) par le biais d'un ressort de dépassement de course (31) pour, après de la réalisation d'une course de dosage, réaliser un dépassement de course par la poursuite du déplacement de l'élément d'actionnement (4) dans la direction d'actionnement contre la force de ressort du ressort de dépassement de course (31) jusqu'à ce que la butée d'extrémité (32) soit atteinte par l'élément de butée d'extrémité (38) et pour déplacer vers le bas le prolongement de délivrance (74).
  3. Pipette selon la revendication 2, dans laquelle le corps de butée (8) supérieur peut être déplacé dans la partie supérieure de boîtier (2) et la partie supérieure de boîtier (2) présente un système de réglage (4, 9, 10) couplé au corps de butée (8) supérieur pour le réglage de la position du corps de butée (8) supérieur dans la partie supérieure de boîtier (2) et pour la détermination de la course de dosage.
  4. Pipette selon la revendication 2 ou 3, dans laquelle l'élément de butée (25) est un collet sur la périphérie de la tige de course (6), la tige de course (6) s'étend à travers un canal de traversée (7) supérieur du corps de butée (8) supérieur et un canal de traversée (28) du corps de butée (27) inférieur, et le collet (25) est disposé entre le corps de butée (8) supérieur et le corps de butée (27) inférieur.
  5. Pipette selon l'une des revendications 1 à 4, dans laquelle le système de découplage (44) présente deux éléments de découplage (39, 40) dont un élément de découplage (40) est raccordé au système d'entraînement (6) et l'autre élément de découplage (39) est raccordé à l'élément d'entraînement (34), les éléments de découplage (39, 40) étant maintenus assemblés de façon détachable dans une position de raccordement définie et pouvant être éloignés l'un de l'autre après qu'une force de retenue définie a été surmontée et pouvant être ramenés à la position de raccordement.
  6. Pipette selon la revendication 5, dans laquelle les éléments de découplage comprennent deux aimants ou un aimant et une pièce antagoniste ferromagnétique et/ou un système de cliquet et/ou un système de ressort qui retiennent les éléments de découplage de façon détachable dans la position de raccordement.
  7. Pipette selon l'une des revendications 1 à 6, dans laquelle l'élément d'entraînement (34) est une douille dont l'extrémité inférieure repose sur l'élément de contact (63), et un des éléments de découplage (39, 40) du système de découplage (44) est disposé dans la douille et est raccordé de façon fixe à celle-ci et est constitué en tant qu'élément de découplage (39) supérieur avec un trou traversant supérieur, et l'autre élément de découplage (40) du système de découplage (44) est disposé de façon déplaçable dans la douille et est constitué en tant qu'élément de découplage (40) qui est fixé sur l'extrémité inférieur d'une tige de course (6) qui s'étend à partir du haut dans la douille et à travers le trou traversant supérieur, et la douille s'étend à travers un trou de passage (33) de la butée d'extrémité (32) et présente un autre collet (38) faisant saillie radialement vers l'extérieur qui limite le mouvement de l'élément d'entraînement (34) vers le bas par le placement sur le côté supérieur de la butée d'extrémité (32).
  8. Pipette selon la revendication 7, dans laquelle, sous l'élément de découplage (40) inférieur, un ressort de découplage (43) est disposé dans la douille par le biais duquel l'élément de découplage (40) inférieur est supporté dans la douille.
  9. Pipette selon la revendication 7 ou 8, dans laquelle la douille présente un tronçon de douille (36) inférieur et un tronçon de douille (35) supérieur, le tronçon de douille (36) inférieur présentant un diamètre plus faible que le tronçon de douille (35) supérieur, s'étendant à travers l'orifice de boîtier (67) supérieur de la partie inférieure de boîtier (3) et reposant par l'extrémité inférieure sur l'élément de contact (63), et les éléments de découplage (39, 40) étant disposés dans le tronçon de douille (35) supérieur.
  10. Pipette selon l'une des revendications 1 à 9, dans laquelle le ressort de dépassement de course (31) est supporté sur un palier de ressort de dépassement de course (32) en forme de disque annulaire circulaire dans la partie supérieure de boîtier, la douille est conduite à travers le trou de passage (33) du palier de ressort de dépassement de course (32), et le palier de ressort de dépassement de course (32) est la butée d'extrémité pour le collet (38) de la douille.
  11. Pipette selon l'une des revendications 1 à 10, dans laquelle le corps de butée (27) inférieur repose sur l'extrémité d'actionnement (78) du prolongement de délivrance (74) par un bord extérieur dépassant latéralement à l'extrémité du dépassement de course ou au début de la poursuite du déplacement de l'élément d'actionnement (4) dans la direction d'actionnement pour déplacer vers le bas le prolongement de délivrance (74) en tant qu'élément d'entraînement.
  12. Pipette selon l'une des revendications 1 à 11, dans laquelle le prolongement de délivrance (74) est supporté dans la partie supérieure de boîtier (2) par un ressort de délivrance (81) disposé au-dessous du palier de ressort de dépassement de course (32) dans la partie supérieure de boîtier (2).
  13. Pipette selon la revendication 12, dans laquelle le ressort de délivrance (81) est supporté en haut sur un palier de ressort de délivrance (77) supérieur disposé au-dessous du palier de ressort de dépassement de course (32) sur le prolongement de délivrance (74), et supporté en bas sur un palier de ressort de délivrance (80) inférieur disposé dans la partie supérieure de boîtier (2) et raccordé de façon fixe à la partie supérieure de boîtier .
  14. Pipette selon l'une des revendications 1 à 13, dans laquelle le curseur de délivrance (72) est une douille de délivrance qui est guidée à l'extérieur sur la partie inférieure de boîtier (3), et/ou le prolongement de délivrance (74) présente plusieurs tiges de délivrance (75) parallèles qui sont raccordées les unes aux autres à distance du curseur de délivrance (72) par un palier de ressort de délivrance (77) supérieur en forme d'anneau circulaire.
  15. Pipette selon l'une des revendications 1 à 14, dans laquelle le curseur de délivrance (72) et le prolongement de délivrance (74) sont raccordés l'un à l'autre par au moins un encliquetage.
EP13000386.6A 2012-02-07 2013-01-26 Pipette Active EP2626134B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
PL13000386T PL2626134T3 (pl) 2012-02-07 2013-01-26 Pipeta

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE102012002169.0A DE102012002169B4 (de) 2012-02-07 2012-02-07 Pipette

Publications (3)

Publication Number Publication Date
EP2626134A2 EP2626134A2 (fr) 2013-08-14
EP2626134A3 EP2626134A3 (fr) 2013-11-06
EP2626134B1 true EP2626134B1 (fr) 2017-03-01

Family

ID=47623907

Family Applications (1)

Application Number Title Priority Date Filing Date
EP13000386.6A Active EP2626134B1 (fr) 2012-02-07 2013-01-26 Pipette

Country Status (4)

Country Link
EP (1) EP2626134B1 (fr)
CN (1) CN103447104B (fr)
DE (1) DE102012002169B4 (fr)
PL (1) PL2626134T3 (fr)

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CN113926500A (zh) * 2020-07-14 2022-01-14 埃佩多夫股份公司 与具有集成的尖端活塞的移液器尖端一起使用的移液器

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DE102014109345A1 (de) * 2014-07-04 2016-01-07 Eppendorf Ag Pipette zum Betätigen einer Spritze
DE102016121813A1 (de) * 2016-11-14 2018-05-17 Ika-Werke Gmbh & Co. Kg Pipette und Verfahren zum Einstellen eines zu pipettierenden Volumens einer Pipette
DE202017101008U1 (de) * 2017-02-23 2018-05-24 Brand Gmbh + Co Kg Dispenser und System zum Aufnehmen und Abgeben von Fluidvolumina
EP3680017B1 (fr) * 2019-01-08 2021-07-21 Eppendorf AG Pipette destinée à une utilisation avec une pointe de pipette

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DE2319175A1 (de) * 1973-04-16 1974-10-31 Oxford Lab Detail-kolbenpipette
DE2549477C3 (de) * 1975-11-05 1982-01-07 Eppendorf Gerätebau Netheler + Hinz GmbH, 2000 Hamburg Pipettiervorrichtung
EP0181957A1 (fr) * 1984-11-20 1986-05-28 HTL High-Tech Lab Herstellung und Vertrieb medizinisch technischer Erzeugnisse GmbH Pipette à dose réglable
EP0364621B1 (fr) * 1988-10-21 1992-09-30 Eppendorf-Netheler-Hinz Gmbh Dispositif pour pipetter
DE4014333A1 (de) * 1990-05-04 1991-11-28 Eppendorf Geraetebau Netheler Pipettiervorrichtung
DE19708151C2 (de) * 1997-02-28 1999-05-27 Eppendorf Geraetebau Netheler Pipettiervorrichtung
DE102004003434B4 (de) 2004-01-21 2006-06-08 Eppendorf Ag Pipettiervorrichtung mit einer Verdrängungseinrichtung und einer damit lösbar verbundenen Antriebseinrichtung
DE102005041183B3 (de) * 2005-08-31 2007-01-04 Eppendorf Ag Pipettiervorrichtung

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Publication number Priority date Publication date Assignee Title
CN113926500A (zh) * 2020-07-14 2022-01-14 埃佩多夫股份公司 与具有集成的尖端活塞的移液器尖端一起使用的移液器
CN113926500B (zh) * 2020-07-14 2023-05-09 埃佩多夫欧洲股份公司 与具有集成的尖端活塞的移液器尖端一起使用的移液器

Also Published As

Publication number Publication date
CN103447104B (zh) 2016-05-18
EP2626134A2 (fr) 2013-08-14
DE102012002169B4 (de) 2017-02-09
EP2626134A3 (fr) 2013-11-06
CN103447104A (zh) 2013-12-18
PL2626134T3 (pl) 2017-08-31
DE102012002169A1 (de) 2013-08-08

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