EP1557222B1 - Pipette avec éjecteur d'embouts de pipette - Google Patents

Pipette avec éjecteur d'embouts de pipette Download PDF

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Publication number
EP1557222B1
EP1557222B1 EP05000212.0A EP05000212A EP1557222B1 EP 1557222 B1 EP1557222 B1 EP 1557222B1 EP 05000212 A EP05000212 A EP 05000212A EP 1557222 B1 EP1557222 B1 EP 1557222B1
Authority
EP
European Patent Office
Prior art keywords
spring
attachment
pipetting
drive
pipetting device
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP05000212.0A
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German (de)
English (en)
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EP1557222A3 (fr
EP1557222A2 (fr
Inventor
Herbert Belgardt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf SE
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Publication date
Application filed by Eppendorf SE filed Critical Eppendorf SE
Priority to EP10011574.0A priority Critical patent/EP2263800A3/fr
Publication of EP1557222A2 publication Critical patent/EP1557222A2/fr
Publication of EP1557222A3 publication Critical patent/EP1557222A3/fr
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Publication of EP1557222B1 publication Critical patent/EP1557222B1/fr
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/0275—Interchangeable or disposable dispensing tips
    • B01L3/0279—Interchangeable or disposable dispensing tips co-operating with positive ejection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/08—Ergonomic or safety aspects of handling devices
    • B01L2200/087—Ergonomic aspects
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/025—Displaying results or values with integrated means
    • B01L2300/026—Drum counters

Definitions

  • the invention relates to a pipetting device with at least one approach for attaching a pipette tip and a discharge device for releasing a pipette tip of the approach.
  • Pipetting devices are used in particular in the laboratory for dosing liquids. These are picked up in pipette tips through a tip opening and dispensed.
  • a displacement device for a gas is integrated in the pipetting device and connected in a communicating manner with the pipette tip through the approach.
  • the displacement device is usually a cylinder with a piston displaceable therein.
  • the pipette tips are releasably connected to the neck so that they can be replaced with a fresh tip after use. As a result, contamination can be avoided at subsequent dosages.
  • Single-use pipette tips are available inexpensively from plastic.
  • the approach for attaching pipette tips is often a cylindrical or conical projection with respect to a base body or a housing, on which a pipette tip with a mating Aufsteckö réelle or recording is clamped. This can be done without touching the pipette tip by pressing the projection into the plug-in opening of the pipette tip provided in a holder.
  • pipetting devices In order to avoid contamination of the users, pipetting devices have a discharge device with a drive device and an ejector. By Actuating the drive means, the ejector is displaced so that it releases the pipette tip of the approach, without having to be handled by the user.
  • the drive device has a mechanism which must be manually operated by means of an actuating button to release the pipette tip from the neck.
  • drive devices with an electric motor drive. The release of the pipette tip from the neck can require considerable effort, especially with pipette tips firmly clamped onto the neck.
  • a pipetting device having a pipette tip fitting approach which is a shaft protruding portion of a shaft which is rotatable in the shaft.
  • the pipetting device has a rotary drive for the shaft.
  • the end facing the approach of the shaft is designed as a cam surface.
  • the pipette tip also has a cam surface at the plug-in end.
  • pipetting devices and associated pipette tips are known which have an internal shoulder to limit changes in Aufsteckkraft and, accordingly, the force required to operate the Abscheinrichtting.
  • These pipetting systems have the disadvantage that the use of the pipette tips is often limited to the associated pipetting devices and vice versa. The user is limited by this.
  • US 2002 / 001,545 A1 discloses a pipetting device which, when a pipette tip is placed on a hub, stores energy that is released from the hub for ejecting the pipette tip.
  • a mechanism is additionally provided which limits the attachment force of the pipette tip.
  • a spring-loaded ejection sleeve is provided, through which the approach is passed.
  • To attach the tip of the Abwerfhülse is moved against the spring bias from the end of the approach away until a locking mechanism engages.
  • To control the Aufsteckkraft of the pipette tip of the approach is preferably supported on a prestressed spring, so that they can move in the direction of Aufsteckkraft.
  • the pipette tip presses when plugging initially against the outer end of the ejection sleeve and moves it against the biased spring deeper into the housing. Only in a later phase is the pipette tip clamped on the approach. If the attachment force exceeds the bias of the spring supporting the hub, the hub is moved deeper into the housing, thereby avoiding excessive attachment force. With continued pressing of the pipette tip the ejection sleeve reaches the locking position. If the user continues to press the pipette tip further, the ejection sleeve eventually rests in the housing, limiting the path of the pipette tip and thus the attachment force.
  • the user must therefore always apply all the force required for the automatic ejection of the pipette tip when attaching the pipette tip.
  • This force is significantly higher than the Aufsteckkraft to ensure safe ejection of the pipette tip under all circumstances. It should also be taken into account that by changing the ambient conditions (temperature changes, humidity, sample fluid, etc.), the force required to eject the pipette tip can change. As a result, the energy stored for the ejection process must have a safety margin, but this is not needed for each discard operation. The user must apply the corresponding force.
  • the present invention seeks to provide a pipetting available, which reduces the force required for the operation of the discharge device.
  • a pipette tip is plugged by axial insertion of the approach in the plug-in opening.
  • the Aufsteckkraft is introduced into the spring. If the slip force exceeds a certain value, the spring is elastically deformed until the pipette tip clamped on the shoulder abuts against the stop. As soon as the pipette tip touches the stopper, it can no longer be pushed onto the attachment. As a result, the attachment force of the pipette tip is limited.
  • the Aufsteckkraft is determined by the spring characteristic of the spring or any existing bias of the spring. The spring is so designed and optionally biased that the pipette tip exactly when it with the desired Aufsteckkraft sits on the approach, rests against the stop.
  • the Aufsteckkraft is set so that the pipette tip sits securely on the approach or seals. Since a precise defined Aufsteckkraft is achieved, it is possible to limit the Aufsteckkraft to a low value, in which the required safety of the seat and the seal is just given. Thus, the Aufsteckkraft can be significantly reduced compared to conventional pipetting in which due to the uncertainties when plugging regularly excessive Aufsteckkraft is expended. Accordingly, in the pipetting device according to the invention, the force required for the actuation of the ejection device is reduced. In manually operable ejection devices are by
  • Electromotive operated ejection devices can handle low power engines.
  • the spring is designed and / or biased so that the approach of a defined Aufsteckkraft that can be applied by attaching a pipette tip on the approach, in such a way towards the stop is displaced that the pipette tip abuts the stop.
  • the approach with respect to the pipetting device is stationary and the drive means is only operatively connected to the ejector, so that only the ejector is movable by means of the drive means.
  • the approach is supported directly on the spring.
  • the connection of the approach with a fixedly connected to the body displacement means may be effected by means of a flexible connection, for example by means of a flexible hose or a telescopic pipe connection.
  • the approach is firmly connected to a displacement device - for example with a cylinder of a relative to the main body axially displaceable piston-cylinder device - connected. The displacer is then displaced with the neck. The displacement of the displacement device can be compensated for by the drive device for the displacement device.
  • the cylinder is supported by the spring on the base body, so that the approach is indirectly cushioned over the cylinder.
  • the spring is an extended in the axial direction of the neck coil spring.
  • the coil spring is preferably coaxial with the extension.
  • the displacement device or drive device for the displacement device can advantageously be passed through the coil spring.
  • the pipetting device can be executed with a non-preloaded spring. Then the spring is compressed according to the increase in Aufsteckkraft when attaching the approach to the pipette tips.
  • the spring has a spring bias. As a result, it comes only to an axial displacement of the approach when the Aufsteckkraft exceeds the force to bias the spring. As a result, the Aufsteckkraft required for reaching the stop is defined. Furthermore, this favors particularly compact spring arrangements and short displacement paths of the approach.
  • the stop is axially movable with respect to the approach and a fixed to the body connected limitation for the axial movement of the stop to the body out is available.
  • the stopper is formed on the ejector.
  • the stop is formed by the approach end facing the sleeve.
  • the approach can be determined in various ways in its axial starting position for receiving a pipette tip, for example by connecting the approach with the spring.
  • the initial position of the approach is then defined by the initial position of the spring unloaded by the approach.
  • the approach is supported in the direction away from the spring at a further stop, against which it is pressed by the spring. As a result, the starting position is precisely determined. In addition, no connection between the neck and spring is required.
  • the spring is exchangeable and / or there is a device for adjusting the spring preload. This makes it possible to adapt the attachment and ejection forces to differently shaped or sized pipette tips. The range of application of the pipetting device is thereby increased.
  • the drive device can be actuated counter to the action of a further spring supported on the one hand on a movable part of the drive device and on the other hand on the main body. As a result, the drive device automatically returns to its original position after actuation.
  • a fixed limitation with respect to the main body for the movement of the ejector or the drive device. This is at the same time the limitation for the axial movement of the stop to the main body.
  • the base body on a support or frame.
  • the main body has a housing, optionally with a carrier or frame contained therein.
  • the pipetting device is a hand-held device and / or a stationary device and / or an electrically driven device and / or a (semi-) automaton.
  • the pipetting device is manually guided to the location of sample receptacles and Probenabgabcn and is the Pickup and delivery of liquid and the operation of the discharge device controlled by hand.
  • the drive devices for the displacement device and / or the ejector are designed mechanically and / or electromechanically. The latter also applies to the execution of the pipetting as a stationary device.
  • the pipetting device When the pipetting device is designed as a (semi) automaton, all functions or a part of the functions of the pipetting devices (picking up and dispensing liquid, moving the pipetting devices into positions for picking up and dispensing liquid or pipette tips, picking up and dispensing pipette tips) are automatically carried out ,
  • the pipetting device has a series of parallel lugs for receiving pipette tips. It is a multi-channel pipetting system. Due to the multiplied peak discharge forces, the use of the invention is particularly advantageous here.
  • each approach is supported on the main body via a separate spring.
  • the approaches associated with a common ejector are associated with the approaches associated with a common ejector.
  • the displacement device and the drive device for driving the displaceable boundary of the pipetting device can be connected to each other in a simple manner by mating along a longitudinal axis of the bayonet connection and rotating about the longitudinal axis of the bayonet connection or in a reverse manner separable from each other.
  • the operative connection between the drive member and displaceable boundary is at the same time produced, without requiring special further actions for this purpose.
  • the active compound is released without any special further actions.
  • the invention enables a particularly simple, fast and secure connection and disconnection of the displacement device and the drive device for driving the displaceable boundary, for example during assembly, before autoclaving or other cleaning of the lower part, before replacing the lower part for purposes of changing the working area, Repair etc.
  • the bayonet connection is particularly susceptible to interference.
  • the drive device for driving the displaceable boundary can be designed in various ways. It has technical means to shift the drive member so that this shifts the movable limit of the displacement device.
  • the drive member carries e.g. a linear motion.
  • the drive device for driving the displaceable boundary on a linear drive are e.g. to a directly actuated by key operation stroke rod or to a linearly displaceable via an electric drive motor and a gear lift rod.
  • a pneumatically or hydraulically operated pressure medium cylinder as a drive for the lifting rod, which is actuated by a pneumatic or hydraulic control and a pressure medium reservoir. If the drive member does not perform a linear movement, but a spatial feed movement, the drive means for driving the movable boundary on a corresponding drive.
  • the drive device for driving the movable boundary on a housing in which drive and drive member are arranged.
  • the drive member is a displaceable parallel to the longitudinal axis of the bayonet connection lifting rod of the drive device and has the displacement means connected to the boundary, directed transversely to the lifting rod contact surface, which is pressed by a lifting spring against the end of the lifting rod.
  • the operative connection between the drive member and displaceable boundary is automatically produced when producing the bayonet connection and automatically released when dissolving the bayonet connection.
  • the contact surface is formed on a connected via a rod with the movable boundary pressure element and is the lifting spring designed as a helical spring which is supported at one end on the pressure piece and the other end to the displacement chamber.
  • the bayonet connection can be configured in various ways. Included in particular by the invention is the configuration of the drive device as a male part and the displacement device as a female part of the bayonet connection and vice versa.
  • the drive device has a cylindrical receptacle which has an opening at one end, through which the cylindrical receptacle is accessible in the axial direction from the outside, which has at least one axially directed longitudinal groove which is connected to an annular groove directed in the circumferential direction of the cylindrical receptacle is, and the displacement means on a cylindrical portion at least one outwardly projecting projection, wherein the cylindrical portion in the axial direction of the cylindrical receptacle through the opening in the receptacle and with the projection in the longitudinal groove inserted and screwed with the projection in the annular groove is.
  • the drive means is the female and the displacement means the male part.
  • the annular groove at a distance from the longitudinal groove on a in the axial direction of the receptacle extending boundary wall, to which the projection is rotatable. Reaching the limit indicates to the user that the bayonet connection is made.
  • the annular groove is connected at a distance from the longitudinal groove with a parallel thereto extending L jossnutabexcellent, which ends at a distance from the opening.
  • the annular groove has a ramp-like boundary wall whose distance from the opening increases with increasing distance from the longitudinal groove.
  • the ramp-like course of the boundary wall facilitates the finding of the connection position and the separation of the displacement device from the drive device.
  • the longitudinal groove, the annular groove and optionally the L jossnutabêt is formed in a cylindrical coupling piece which forms the receptacle of the drive means and is fixed in this. This facilitates manufacture, assembly and disassembly.
  • the drive device has a spring which presses against the displacement device connected to the drive device via the bayonet connection. This will secure the bayonet connection.
  • the spring is arranged on a further opening of the receptacle, which is opposite to the opening for the axial insertion of the displacement device. Through the opening displacement and spring act on each other.
  • the spring is a helical spring, which is supported on an inner end face of the coupling piece.
  • the longitudinal groove and / or annular groove and / or the L jossnutabites open to the other opening.
  • the displacement device is a piston-cylinder device with a cylinder and a piston displaceable therein and the piston has the displaceable boundary.
  • Other displacement devices are also included by the invention, for example, a displacement chamber with an elastic wall forming the displaceable boundary.
  • a piston-cylinder device is actuated, for example, by a linear drive device.
  • a corresponding actuation is possible with a displacement chamber with an elastic wall.
  • the latter can also be controlled via a drive device with a spatial drive movement.
  • the approach is aligned coaxially to the longitudinal axis of the bayonet connection. According to a further embodiment, the approach is firmly connected to the displacement device.
  • the pipetting device has a discharge device for ejecting a pipette tip from the attachment, which has a discharge drive arranged on the drive device, an ejector arranged on the displacement device and a directed in the direction of the longitudinal axis of the bayonet connection, detachable axial clamping connection between discharge drive and ejector.
  • the clamping connection can be produced simultaneously with the production of the bayonet connection with the phase of the axial collapse of the displacement device and the drive device and can be released in the reverse direction.
  • the ejector drive a projecting from the drive device parallel to the bayonet release rod and the ejector to a neck parallel, axial bore, with which the discharge rod is press-connected.
  • the ejector is guided on the displacement device.
  • the ejector is a sleeve guided on the displacement device.
  • the pipetting device is a hand-held device and / or a stationary device and / or an electrically driven device and / or a (semi-) automaton.
  • the pipetting device is manually guided to the location of the sample receptacles and sample dispensing, and the picking and dispensing of liquid and the actuation of the ejection device are controlled manually.
  • the drive devices for the displacement device and / or the ejector are designed mechanically and / or electromechanically. The latter also applies to the execution of the pipetting as a stationary device.
  • the pipetting device When the pipetting device is designed as a (semi) automaton, all functions or a part of the functions of the pipetting devices (picking up and dispensing liquid, moving the pipetting devices into positions for picking up and dispensing liquid or pipette tips, picking up and dispensing pipette tips) are automatically carried out ,
  • the pipetting device has a series of parallel lugs for receiving pipette tips. It is a multi-channel pipetting system. Each approach of the pipetting device is associated with a separate displacement means or a common, which is connected via a bayonet connection with the drive means. It may be a common drive device for all displacement devices.
  • top, bottom, “horizontal”, “vertical” and “front”, “rear” refer to the orientations of the pipetting devices according to the drawings. These are orientations of the pipetting devices in which the pipette tip is arranged with its tip opening at the bottom, in order to receive liquid from a vessel located below the pipetting device or to deliver it into such a vessel.
  • the pipetting device according to Fig. 1 and 2 has an elongated, shaped as a handle housing 1 with an upper housing part 2 and a lower housing part 3.
  • the upper housing part 2 with all the parts contained forms a drive means and the lower housing part 3 with all parts contained a displacement device.
  • the upper housing part 2 forms a base body. It has at the top a screw cap 4. From this, an adjusting sleeve 5 projects upwards.
  • the adjusting sleeve 5 is mounted axially non-displaceably and rotatably in the upper housing part 2.
  • a push button 6 is arranged, which protrudes further upwards.
  • the push button 6 is connected to a lifting rod 7, which is passed through a spindle 8 in the upper housing part 2.
  • the spindle 8 is screwed into an internal thread 9 of a bearing body 10 fixed in the housing upper part 2.
  • the spindle 8 has a rotatably connected to her driver 11 above.
  • the driver 11 has on the circumference two diametrically opposed radial projections 12.
  • the radial projections 12 engage in - not shown - axially extending grooves of the adjusting sleeve 5 a.
  • the spindle 8 has an end stop 13 in the form of radially outwardly projecting ribs. In the position shown, the end stop 13 is located a short distance below a shoulder 14 of the bearing body 10, with which it cooperates.
  • the lifting rod 7 has a flange 15 which rests in the position shown at the bottom of the spindle 8.
  • a spring plate 16 is arranged, which engages with a collar 17 in the bearing body 10.
  • the spring plate 16 has an axially projecting, sleeve-shaped bearing portion 18 below, through which the lifting rod 7 is passed.
  • the pipetting device on a spring, not shown, which pushes the lifting rod 7 upwards, so that the flange 15 rests against the underside of the spindle 8.
  • a coil spring between flange 15 and spring plate 16 is arranged.
  • a coupling piece 19 is fixed in the housing.
  • This has several pockets inside 20. These have an axial over the Further, they have at the upper end of the coupling piece 19 a over a small part of the circumference of the coupling piece 19 extending annular groove 22. This has at a distance from the upper end of the coupling piece 19 below a ramp-like boundary wall, starting of the longitudinal groove 21 is increasingly approximated to the upper end of the coupling piece 19.
  • the pockets 20 at the other end of the annular groove 22 have a short axial longitudinal groove portion 23 which terminates at a distance from the upper end of the coupling piece 19 in the coupling piece 19.
  • a spring 24 is arranged under bias, which is stirred as a helical spring.
  • the adjusting sleeve 5 has on the circumference a sprocket 25 which cooperates with a gear 26 which drives a counter 27 with a plurality of superimposed on an axis 28 Zählwerkssonn 29.
  • the Zählwerksson 29 each have numerals from 0 to 9. the lower Zählwerksrad 29 is driven by the gear 26.
  • the overlying Zählwerksson 29 are further rotated in each case by one digit when the underlying Zählwerksrad 29 passes from 9 to 0.
  • the lower housing part 3 can be detachably connected to the upper housing part 2.
  • the lower housing part 3 on the mantle of an upper cylindrical portion 30 a plurality of outwardly projecting ribs 31 which extend in the axial direction of the cylindrical portion 30.
  • the lower housing part 3 has below the cylindrical portion 30 a plurality of conical sections 31.1 to 33 of different length and conicity, which emerge from the drawing.
  • the conical section 33 is down with a long, light conical projection 34 connected to attach a pipette tip. This in turn has a short, more conical Aufsteckende 35 below.
  • the lower housing part 3 houses a displacement device in the form of a piston-cylinder unit 36. This has a conical portion 32 arranged in the cylinder 37, in which a piston 38 dips.
  • the piston 38 is connected at the top via a piston rod 39 with a pressure piece 40.
  • the piston 38 forms a displaceable boundary of the cylinder 37.
  • the lower housing part 3 has a piston holder 41, which bridges the cylindrical portion 30 above.
  • the piston holder 41 has at the top a central passage 42, through which a lower portion of the piston 7 can be axially guided.
  • a lifting spring 43 is arranged, which is designed as a helical spring. By the lifting spring 43, the piston 38 and the piston rod 39 are passed.
  • the lifting spring 43 is biased and presses the pressure piece 40 against the piston holder 41, so that the piston 38 is pulled out of the cylinder 37 maximum.
  • a connecting channel 44 extends through the lug 34 and connects the cylinder 37 with an orifice in the push-on end 35.
  • the pipetting device has a discharge device 45.
  • the discharge device 45 has in the upper housing part 2 in addition to the adjustment knob 5, an operating button 46.
  • the actuating button 46 is connected to a discharge bar 47 which extends parallel to the lifting rod 7 through the upper housing part 2 therethrough.
  • a transmission 48 is integrated in the discharge rod 47.
  • the gear 48 converts an axial actuating stroke of the actuating knob 46 into a smaller drive stroke with increased force.
  • Suitable transmissions 48 are in the EP 0 992 288 A described generally in the general part of the description and specifically in the description of the figures, which are incorporated by reference into the present application.
  • the discharge rod 47 is supported via a further coil spring 49 in the upper housing part 2, so that the actuating button 46 is pressed into the initial position shown, in which it can be pressed against the action of the other coil spring 49.
  • the lower end of the discharge rod 47 projects into a receptacle 50 at the lower end of the housing upper part 2.
  • the discharge device 45 has on the lower housing part 3 a discharge sleeve 51. This is guided on the cylindrical portion 30, the conical portion 32 and the projection 34. Accordingly, the contour of the discharge sleeve 51 is equalized to the contours of the aforementioned sections of the housing lower part 3. In this case, the discharge sleeve 51 has internal steps 52, 53 which limit the sliding of the discharge sleeve 51 upwards by abutting against conical sections 31, 33 of the housing lower part 3.
  • the discharge sleeve 51 has laterally at the upper edge a projection 54 which has an axial bore 55 for pressing in the lower end of the discharge rod 47.
  • the pipetting device can be used as follows:
  • Upper housing part 2 and lower housing part 3 can be connected by axial insertion and rotation of the lower part 3 in the coupling piece 19. This will create a bayonet connection produced.
  • the ribs 31 are inserted into the longitudinal grooves 21, rotated by the annular grooves 22 and inserted into the short Lssensnutenabête 23.
  • the spring 24 presses against the upper edge of the cylindrical portion 30, whereby the lower housing part 3 is fixed in its fastening position, in which the ribs 31 abut against the lower ends of the Lssensnutenabroughe 23, which form a stop.
  • the discharge sleeve 51 is pressed with the bore 55 on the lower portion of the discharge rod 47. The dismantling of the upper housing part 2 and lower housing part 3 can be done in the reverse manner.
  • the lifting rod 7 engages through the passage 42 and lies with its lower end against the pressure piece 40.
  • the adjustment sleeve 5 is rotated until the counter 27 indicates the desired volume.
  • the driver 11 is rotated due to the radial projections 12.
  • the spindle 8 rotates in the internal thread 9 and displaces axially in the upper housing part 2 with entrainment of the flange 15 and thus the lifting rod 7.
  • the radial projections 12 displace axially along the grooves on the inside of the adjusting 5. In this way, the stroke of the lifting rod 7 changed, which is executable upon actuation of the push button 6.
  • a pipette tip 56 is clamped on the lower end of the projection 34.
  • the pipette tip 56 has a lower tip opening 57 for receiving and dispensing liquid.
  • the push button 6 For pipetting the push button 6 is pressed down, so that the piston 38 displaces air from the cylinder 37. Then, the pipette tip 56 is immersed with its lower tip opening 57 in the liquid to be pipetted. Thereafter, the push button 6 is released and the lifting rod 7 is returned to its original position under spring action. Similarly, the piston 38 returns under the action of the spring 43 to its original position. In this case, the piston 38 sucks liquid through the lower tip opening 57 into the pipette tip 56.
  • the lower tip opening 57 of the pipetting device is aligned to a delivery location.
  • the liquid contained in the pipette tip 56 is delivered by depressing the push button 6, re-immersing the piston 38 in the cylinder 37 and expressing air through the connecting channel 44. After releasing the operating knob 6, the lifting rod 7 and the piston 38 again by spring force back to the starting position.
  • a dispensing head 61 of a multi-channel pipetting device has a housing 62 in which eight parallel piston-cylinder devices 63 are arranged in a row.
  • Each piston-cylinder device 63 has a cylinder 64 into which a piston 65 dips.
  • each cylinder 64 designed as a helical spring spring 66 is guided, which rests on the bottom of a circumferential projection 67 of the cylinder 64.
  • the cylinder 64 is guided in a guide rail 68.
  • each cylinder 64 is connected below the peripheral projection 67 with a conical projection 69 for attaching a pipette tip 70.
  • the cylinders 64 are passed through a fixedly arranged in the housing 62 support plate 71.
  • the springs 66 are supported on the underside of the support plate 71.
  • the cylinders 64 have a further circumferential projection above, which forms a spring bearing 72.
  • Each spring bearing 72 is engaged by a console, not shown, which projects from the inside of the wall of the housing 62.
  • pistons 65 are held at the top in a piston receptacle 73 in the form of a traverse.
  • the piston receptacle 73 has a pin 74 at the top for connection to a lifting rod, not shown, which leads to a drive for the piston 65.
  • pistons 65 designed as helical springs springs 75 are guided, which are supported on the bottom of the spring bearing 72 and are supported at the top of the underside of the piston seat 73.
  • an ejector 76 is mounted in the form of an angled plate. This has in the lower horizontal leg 77 eight holes through which the lugs 69 are passed.
  • the vertical leg 78 is connected via a shaft 79 with a discharge bar, not shown.
  • the pistons 65 are guided by a piston seal 80 in the cylinder 64.
  • the dispensing head 61 is connected to the lifting rod and the discharge rod of a drive device, not shown.
  • the lugs 69 are moved upwardly within the housing 62 as the attachment forces exceed the forces that bias the springs 66.
  • the further attaching movement is stopped because the ejector 76 abuts against the underside of the guide rail 68 with the upper side of its horizontal leg 77.
  • the Aufsteck the forces required to eject the pipette tips 70 are limited.
  • the pistons 65 are moved by means of the lifting rod connected to the pin 74.
  • For the ejection of the pipette tips 70 of the shaft 79 is moved by means of the lifting rod down, so that the ejector 76 is moved down and with its leg 77, the pipette tips 70 from the lugs 69 depressed.

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
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Claims (31)

  1. Dispositif de pipetage avec
    • un corps de base (2),
    • au moins une saillie (34), en saillie du corps de base (2) et montée sur le corps de base (2) de façon axialement mobile pour y mettre un embout de pipette (56),
    • un ressort (24) à travers duquel la saillie (34) est supportée sur le corps de base (2),
    • une butée (59) associée à la saillie (34), au-delà de laquelle la saillie (34) fait axialement saillie quand elle n'est pas chargée en direction du ressort (24), la butée (59) étant axialement mobile relatif à la saillie (34), et une borne (60) fixement reliée au corps de base (2) existante pour un mouvement axial de la butée (59) en direction du corps de base (2),
    • dans lequel le ressort (24) est configuré et/ou précontraint tellement que la saillie (34) peut être déplacée en direction de la butée (59) par une force de mise en place définie qui se laisse appliquer en mettant un embout de pipette (56) sur la saillie (34), de sorte que l'embout de pipette (56) serré sur la saillie (34) vient en butée contre la butée (59), et
    • un dispositif éjecteur (45) pour détacher l'embout de pipette (56) de la saillie (34), qui a un éjecteur (51) associé à la saillie (34) sur lequel la butée (59) est formée, saillie (34) et éjecteur (51) étant mobiles relatif de l'une à l'autre, et qui a un dispositif d'entraînement (46, 47, 48) relié de manière opérationnelle à l'éjecteur (51) pour mouvement relatif d'éjecteur (51) et saillie (34), qui se laisse actionner contre l'action d'un autre ressort (49) supporté dans un côté sur une partie mobile du dispositif d'entraînement (46, 47, 48) et dans l'autre côté sur le corps de base (2), de sorte qu'après actionnement, le dispositif d'entraînement (46, 47, 48) rentre automatiquement dans sa position initiale qui est définie par la borne (60).
  2. Dispositif de pipetage selon la revendication 1, dans lequel la saillie (34) est fixement reliée à un dispositif de déplacement (36).
  3. Dispositif de pipetage selon la revendication 2, dans lequel le dispositif de déplacement (36) est supporté dans le corps de base (2) à travers le ressort (24).
  4. Dispositif de pipetage selon l'une quelconque des revendications 1 à 3, dans lequel le ressort (24) est un ressort hélicoïdal qui s'étend dans la direction axiale de la saillie (34).
  5. Dispositif de pipetage selon l'une quelconque des revendications 1 à 4, dans lequel la butée (59) est axialement mobile par rapport à la saillie (34).
  6. Dispositif de pipetage selon l'une quelconque des revendications 1 à 5, dans lequel la saillie (34) est supportée dans la direction qui l'éloigne du ressort (24) sur une autre butée (23) contre laquelle elle est pressée par le ressort (24).
  7. Dispositif de pipetage selon l'une quelconque des revendications 1 à 6, dans lequel le ressort (24) est remplaçable et/ou il y a un dispositif pour ajuster la précontrainte du ressort.
  8. Dispositif de pipetage selon l'une quelconque des revendications 1 à 7, dans lequel le corps de base (2) a un porteur et/ou un cadre et/ou un boîtier.
  9. Dispositif de pipetage selon l'une quelconque des revendications 1 à 8, qui est un dispositif à main et/ou un dispositif stationnaire et/ou un dispositif électriquement entrainé et/ou un dispositif semi-automatique.
  10. Dispositif de pipetage selon l'une quelconque des revendications 1 à 9, qui a une série de saillies parallèles (69) pour le logement d'embouts de pipette (70).
  11. Dispositif de pipetage selon la revendication 10, dans lequel chaque saillie (69) est supportée sur le corps de base (62) par un ressort séparé (66).
  12. Dispositif de pipetage selon la revendication 10 ou 11, dans lequel un éjecteur commun (76) est associé aux saillies (69).
  13. Dispositif de pipetage selon l'une quelconque des revendications 1 à 12, avec
    • un dispositif de déplacement (36) avec une chambre de refoulement (37) ayant une borne mobile (38), une saillie (34) pour liaison à un embout de pipette (56) et un canal de liaison (44) entre la chambre de refoulement (37) et l'extrémité libre de la saillie (34),
    • un dispositif d'entraînement (6, 7, 8) pour entraîner la borne mobile (38) du dispositif de déplacement (36) par un élément d'entraînement (7), qui a une liaison active détachable avec la borne mobile (38), et
    • une liaison à baïonnette (19, 22, 30, 31) entre le dispositif d'entraînement (6, 7, 8) et le dispositif de déplacement (36), qui se laisse établir en faisant la liaison active entre l'élément d'entraînement (7) et la borne mobile (38) et est détachable en détachant la liaison active entre l'élément d'entraînement (7) et la borne mobile (38).
  14. Dispositif de pipetage selon la revendication 13, dans lequel l'élément d'entraînement (7) est une tige de levage du dispositif d'entraînement (6, 7, 8) déplaçable en parallèle à l'axe longitudinal de la liaison à baïonnette, et le dispositif de déplacement (36) a une surface de contact reliée à la borne (38) et dirigée transversalement à la tige de levage (7) qui est poussée contre l'extrémité de la tige de levage (7) par un ressort de levage (43).
  15. Dispositif de pipetage selon la revendication 15, dans lequel la surface de contact est formée sur une pièce d'appui (40) reliée à la borne (38) via une barre (39) et est supportée sur la plaque de contact (40) dans l'une extrémité, et dans l'autre extrémité sur la chambre de refoulement (37) via un ressort de levage (43) formé comme un ressort hélicoïdal.
  16. Dispositif de pipetage selon l'une quelconque des revendications 13 à 15, dans lequel le dispositif d'entraînement (6, 7, 8) a un logement cylindrique (19) qui a une ouverture dans l'une extrémité, à travers laquelle le logement cylindrique (19) est accessible de l'extérieur en la direction axiale, qui a au moins une rainure longitudinale (21) axialement dirigée qui est reliée à une rainure annulaire (22) dirigée en la direction de circonférence du logement cylindrique (19) et dans lequel le dispositif de déplacement (36) a sur une portion cylindrique (30) au moins une saillie (31) faisant saillie vers l'extérieur, la portion cylindrique (30) se laissant insérer dans le logement (19) à travers l'ouverture dans le sens axial du logement cylindrique (19) et avec la saillie (31) dans la rainure longitudinale (21) et se laissant insérer avec la saillie (31) dans la rainure annulaire (22) par rotation.
  17. Dispositif de pipetage selon l'une quelconque des revendications 13 à 16, dans lequel la rainure annulaire (22) a une paroi de limitation qui s'étend dans la direction axiale du logement (19) dans une distance de la rainure longitudinale (21) et jusqu'à' laquelle la saillie (31) se laisse tourner.
  18. Dispositif de pipetage selon la revendication 13 ou 17, dans lequel la rainure annulaire (22) est reliée, dans une distance de la rainure longitudinale (21), à une partie de rainure longitudinale (23) qui se déroule en parallèle à celle-ci et finit en une distance de l'ouverture.
  19. Dispositif de pipetage selon l'une quelconque des revendications 16 à 18, dans lequel la rainure annulaire (22) a une paroi de limitation en forme de rampe, dont la distance de l'ouverture accroit avec distance croissante de la rainure longitudinale (21).
  20. Dispositif de pipetage selon l'une quelconque des revendications 16 à 19, dans lequel la rainure longitudinale (21), la rainure annulaire (22) et au choix la partie de rainure longitudinale (23) sont réalisées avec une pièce de couplage cylindrique (19) qui forme le logement du dispositif d'entraînement (6, 7, 8) et est fixée dans celui-ci.
  21. Dispositif de pipetage selon l'une quelconque des revendications 13 à 20, dans lequel le dispositif d'entraînement (6, 7, 8) comporte un ressort (24) qui pousse contre le dispositif de déplacement (36) relié au dispositif d'entraînement (6, 7, 8) à travers la liaison à baïonnette (19, 22, 30, 31).
  22. Dispositif de pipetage selon la revendication 21, dans lequel le ressort (24) est arrangé sur une autre ouverture du logement (19) qui fait face à l'ouverture pour l'introduction axiale du dispositif de déplacement (36).
  23. Dispositif de pipetage selon la revendication 21 ou 22, dans lequel le ressort (24) est un ressort hélicoïdal qui est supporté par un côté avant de la pièce de couplage (19) situé à l'intérieur.
  24. Dispositif de pipetage selon l'une quelconque des revendications 22 à 23, dans lequel la rainure longitudinale (21) et/ou la rainure annulaire (22) et/ou la partie de rainure longitudinale (23) sont ouvertes en direction de l'autre ouverture.
  25. Dispositif de pipetage selon l'une quelconque des revendications 13 à 24, dans lequel le dispositif de déplacement (36) est une unité piston/cylindre avec un cylindre (37) et un piston (38) mobile dans ceci, et le piston (38) comporte la borne mobile.
  26. Dispositif de pipetage selon l'une quelconque des revendications 13 à 25, dans lequel la saillie (34) est dirigée coaxialement à l'axe longitudinal de la liaison à baïonnette (19, 22, 30, 31).
  27. Dispositif de pipetage selon l'une quelconque des revendications 13 à 26, dans lequel la saillie (34) est fixement reliée à la chambre de refoulement (37).
  28. Dispositif de pipetage selon l'une quelconque des revendications 13 à 27, avec un dispositif d'éjection (45) pour détacher un embout de pipette (56) de la saillie (34), qui a un entraînement d'éjection (46, 47, 48) agencé sur le dispositif d'entraînement (6, 7, 8), un éjecteur (51) agencé sur le dispositif de déplacement (36), et une liaison à serrage axial détachable entre l'entraînement d'éjection (45) et l'éjecteur (51), dirigée en la direction de l'axe longitudinal de la liaison à baïonnette (19, 22, 30, 31).
  29. Dispositif de pipetage selon la revendication 28, dans lequel l'entraînement d'éjection (45) a une tige d'éjection (47) faisant saillie du dispositif d'éjection en parallèle à la liaison à baïonnette (19, 22, 30, 31), et l'éjecteur (51) a un perçage (55) axial en parallèle à la saillie (34) qui est relié à la tige d'éjection (47) par pression.
  30. Dispositif de pipetage selon la revendication 29, dans lequel l'éjecteur (51) est guidé sur le dispositif de déplacement (36).
  31. Dispositif de pipetage selon l'une quelconque des revendications 13 à 30, dans lequel l'éjecteur (51) est une douille guidée sur le dispositif de déplacement (36).
EP05000212.0A 2004-01-21 2005-01-07 Pipette avec éjecteur d'embouts de pipette Active EP1557222B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP10011574.0A EP2263800A3 (fr) 2004-01-21 2005-01-07 Pipette avec éjecteur d'embouts de pipette

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102004003433A DE102004003433B4 (de) 2004-01-21 2004-01-21 Pipettiervorrichtung mit einer Abwurfeinrichtung für Pipettenspitzen
DE102004003433 2004-01-21

Related Child Applications (2)

Application Number Title Priority Date Filing Date
EP10011574.0A Division EP2263800A3 (fr) 2004-01-21 2005-01-07 Pipette avec éjecteur d'embouts de pipette
EP10011574.0A Division-Into EP2263800A3 (fr) 2004-01-21 2005-01-07 Pipette avec éjecteur d'embouts de pipette

Publications (3)

Publication Number Publication Date
EP1557222A2 EP1557222A2 (fr) 2005-07-27
EP1557222A3 EP1557222A3 (fr) 2007-05-09
EP1557222B1 true EP1557222B1 (fr) 2014-11-12

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EP10011574.0A Withdrawn EP2263800A3 (fr) 2004-01-21 2005-01-07 Pipette avec éjecteur d'embouts de pipette

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DE (1) DE102004003433B4 (fr)

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Also Published As

Publication number Publication date
EP2263800A3 (fr) 2014-05-21
US7434484B2 (en) 2008-10-14
US20050155439A1 (en) 2005-07-21
EP2263800A2 (fr) 2010-12-22
DE102004003433A1 (de) 2005-08-25
DE102004003433B4 (de) 2006-03-23
EP1557222A3 (fr) 2007-05-09
EP1557222A2 (fr) 2005-07-27

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