EP1390542B1 - Nucleic acid detection method - Google Patents

Nucleic acid detection method Download PDF

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Publication number
EP1390542B1
EP1390542B1 EP02738330A EP02738330A EP1390542B1 EP 1390542 B1 EP1390542 B1 EP 1390542B1 EP 02738330 A EP02738330 A EP 02738330A EP 02738330 A EP02738330 A EP 02738330A EP 1390542 B1 EP1390542 B1 EP 1390542B1
Authority
EP
European Patent Office
Prior art keywords
probe
label
amplification
sequence
acceptor
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP02738330A
Other languages
German (de)
English (en)
French (fr)
Other versions
EP1390542A2 (en
Inventor
Martin Alan Lee
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
UK Secretary of State for Defence
Original Assignee
UK Secretary of State for Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by UK Secretary of State for Defence filed Critical UK Secretary of State for Defence
Publication of EP1390542A2 publication Critical patent/EP1390542A2/en
Application granted granted Critical
Publication of EP1390542B1 publication Critical patent/EP1390542B1/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Eye Examination Apparatus (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
  • Geophysics And Detection Of Objects (AREA)
EP02738330A 2001-05-25 2002-05-24 Nucleic acid detection method Expired - Lifetime EP1390542B1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0112868 2001-05-25
GBGB0112868.5A GB0112868D0 (en) 2001-05-25 2001-05-25 Detection system
PCT/GB2002/002443 WO2002097132A2 (en) 2001-05-25 2002-05-24 Nucleic acid detection method

Publications (2)

Publication Number Publication Date
EP1390542A2 EP1390542A2 (en) 2004-02-25
EP1390542B1 true EP1390542B1 (en) 2005-06-29

Family

ID=9915364

Family Applications (1)

Application Number Title Priority Date Filing Date
EP02738330A Expired - Lifetime EP1390542B1 (en) 2001-05-25 2002-05-24 Nucleic acid detection method

Country Status (15)

Country Link
US (2) US7700275B2 (es)
EP (1) EP1390542B1 (es)
JP (1) JP2004532044A (es)
KR (1) KR20040024556A (es)
CN (1) CN1304596C (es)
AT (1) ATE298804T1 (es)
AU (1) AU2002311414B2 (es)
CA (1) CA2448211C (es)
DE (1) DE60204874T2 (es)
DK (1) DK1390542T3 (es)
ES (1) ES2240753T3 (es)
GB (1) GB0112868D0 (es)
HK (1) HK1068651A1 (es)
PT (1) PT1390542E (es)
WO (1) WO2002097132A2 (es)

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DE602005025791D1 (de) * 2004-10-18 2011-02-17 Univ Brandeis Verfahren zur nukleinsäureamplifikation
WO2006121277A1 (en) * 2005-05-09 2006-11-16 Korea Research Institute Of Standards And Science Fret probes for fluorescent detection of the epsps gene
KR100764559B1 (ko) * 2005-05-09 2007-10-08 한국표준과학연구원 Ｅｐｓｐｓ 유전자 검색에 활용할 수 있는 ｆｒｅｔ형광검출용 프로브
CN100420943C (zh) * 2005-06-23 2008-09-24 李卫 Dna分子内杂交-聚丙烯酰胺凝胶电泳方法
US8968700B2 (en) * 2005-08-11 2015-03-03 The Board Of Trustees Of The Leland Stanford Junior University Imaging of protease activity in live cells using activity based probes
US7785786B2 (en) * 2006-01-23 2010-08-31 Quest Diagnostics Investments Incorporated Methods for detecting nucleic acids using multiple signals
CN100526461C (zh) * 2006-07-10 2009-08-12 中国农业科学院棉花研究所 获得植物内含子序列扩增多态性的方法及其专用引物
JP2009044967A (ja) * 2007-08-14 2009-03-05 Sony Corp 二本鎖核酸の形成に係る情報を得る方法
US8658366B2 (en) * 2008-09-18 2014-02-25 Roche Molecular Systems, Inc. Detection of target variants using a fluorescent label and a soluble quencher
US20100129796A1 (en) * 2008-11-24 2010-05-27 Micah Halpern Dye probe fluorescence resonance energy transfer genotyping
GB201004339D0 (en) 2010-03-16 2010-04-28 Enigma Diagnostics Ltd Sequence detection assay
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US9180209B2 (en) 2011-02-28 2015-11-10 The Board Of Trustees Of The Leland Stanford Junior University Non-peptidic quenched fluorescent imaging probes
DE102011117520A1 (de) * 2011-11-03 2013-05-08 Frank Schleifenbaum Sensorsystem für nicht intrusive Druckmessungen
EP4218829A3 (en) 2013-03-15 2023-08-16 The Board of Trustees of the Leland Stanford Junior University Activity-based probe compounds, compositions, and methods of use
EP3859333A1 (en) * 2015-09-10 2021-08-04 InSilixa, Inc. Systems for multiplex quantitative nucleic acid amplification
EP4310504A3 (en) 2016-12-23 2024-05-01 The Board of Trustees of the Leland Stanford Junior University Activity-based probe compounds, compositions, and methods of use
JP7149614B2 (ja) 2017-03-30 2022-10-07 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー ｉｎ ｖｉｖｏ画像化のためのプロテアーゼ活性化コントラスト剤

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CA2170604C (en) * 1993-08-30 2007-03-13 Vikas V. Padhye Nucleic acid purification compositions and methods
EP0720659A1 (en) 1993-09-23 1996-07-10 Zeneca Limited Nucleic acid detection with energy transfer
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US5491063A (en) * 1994-09-01 1996-02-13 Hoffmann-La Roche Inc. Methods for in-solution quenching of fluorescently labeled oligonucleotide probes
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US5858397A (en) * 1995-10-11 1999-01-12 University Of British Columbia Liposomal formulations of mitoxantrone
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WO2004031408A1 (en) * 2002-09-30 2004-04-15 F.Hoffmann-La Roche Ag Oligonucleotides for genotyping thymidylate synthase gene
GB0223563D0 (en) 2002-10-10 2002-11-20 Secr Defence Detection system
US7354706B2 (en) * 2003-09-09 2008-04-08 The Regents Of The University Of Colorado, A Body Corporate Use of photopolymerization for amplification and detection of a molecular recognition event
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Also Published As

Publication number Publication date
CN1527885A (zh) 2004-09-08
ES2240753T3 (es) 2005-10-16
US20100227326A1 (en) 2010-09-09
CA2448211C (en) 2010-12-07
DE60204874T2 (de) 2006-04-27
JP2004532044A (ja) 2004-10-21
PT1390542E (pt) 2005-11-30
AU2002311414B2 (en) 2006-11-09
EP1390542A2 (en) 2004-02-25
CN1304596C (zh) 2007-03-14
KR20040024556A (ko) 2004-03-20
DK1390542T3 (da) 2005-08-08
US20040241679A1 (en) 2004-12-02
DE60204874D1 (de) 2005-08-04
WO2002097132A2 (en) 2002-12-05
GB0112868D0 (en) 2001-07-18
HK1068651A1 (en) 2005-04-29
ATE298804T1 (de) 2005-07-15
US7700275B2 (en) 2010-04-20
WO2002097132A3 (en) 2003-09-12
CA2448211A1 (en) 2002-12-05

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