EP1354211A1 - Dispositif de pipettage - Google Patents

Dispositif de pipettage

Info

Publication number
EP1354211A1
EP1354211A1 EP02734828A EP02734828A EP1354211A1 EP 1354211 A1 EP1354211 A1 EP 1354211A1 EP 02734828 A EP02734828 A EP 02734828A EP 02734828 A EP02734828 A EP 02734828A EP 1354211 A1 EP1354211 A1 EP 1354211A1
Authority
EP
European Patent Office
Prior art keywords
pipette
carrier
cable
pipetting device
hollow profile
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP02734828A
Other languages
German (de)
English (en)
Inventor
Martin Ruedisser
Peter Muerset
Nikolaus Ingenhoven
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tecan Trading AG
Original Assignee
Tecan Trading AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tecan Trading AG filed Critical Tecan Trading AG
Publication of EP1354211A1 publication Critical patent/EP1354211A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
    • G01N2035/1025—Fluid level sensing

Definitions

  • the invention relates to a pipetting device, as seen in chemical, biological and medical laboratories.
  • a generic pipetting device is known in which the cable connecting the liquid detector to a control device runs essentially directly from the pipette tip to the carrier.
  • the position of the cables used is difficult to control, all the more so since they usually have low rigidity. This can cause them to get caught and the
  • the invention is based on the object of improving the known pipetting device in the simplest possible way in such a way that malfunctions due to cables are excluded. This object is achieved by the invention as characterized in the characterizing part of claim 1. Further, inventive features result from the dependent claims.
  • the cables are guided in such a way that they are always under control and cannot interfere with the processes. This is all the more important the more complicated the movements to which the pipettes are subjected.
  • FIG. 1 is a side view of a carrier of a pipetting device according to the invention, with various parts, especially the housing, being at least partially removed,
  • Fig. 3 enlarges a section of a section along III-III in
  • FIG. 1 Fig. 4 enlarges a section of Fig. 3 and
  • the pipetting device comprises (FIGS. 1, 2) an elongated carrier 1 with a two shells 2a, b and a housing arranged above it and connected to the same, comprising a C-profile 3, which is suspended so that it can be moved across a work surface the z.
  • B. microtiter plates are positioned can be moved in a controlled manner.
  • a carriage 4 is mounted in the carrier 1 so as to be longitudinally displaceable and carries eight pipettes 5 arranged in a row. However, more or fewer pipettes 5 can also be arranged in such a row.
  • the carriage 4 can be moved longitudinally in the carrier 1 by parallel movement of two toothed belts 6, 7, rollers 8 arranged on both sides in three rows one above the other rolling on rails 9 fastened in the housing.
  • Each of the pipettes 5 (see also FIGS. 3, 5) is mounted in a vertically displaceable manner on a holder 10 designed as a vertical profile and comprises a pipette housing the vertical hollow profile 12 enclosing a channel 11, which is guided in the holder 10 between two guide arms 13a, b with mutually facing grooves 14 into which webs 15 of the hollow profile 12 engage. It has an external toothing 16 which extends from the upper end to the vicinity of the lower end and with which a drive wheel 17 designed as a gearwheel engages.
  • the same is attached to one of a total of eight profile rods 18 extending over the length of the carrier 1, which are preferably individually driven by a motor (not shown), but cannot be rotated longitudinally, so that it is also displaced by the holder 10.
  • the hollow profile 12 can therefore be raised and lowered by rotating the corresponding profile rod 18 and thus the drive wheel 17, which engages with its external toothing 16.
  • the external toothing 15 breaks off just above the lower end of the hollow profile 12 and it then has an opening 19 through which the channel 11 is accessible.
  • a pipette head 20 is attached, which (see FIG. 4) comprises a base plate 21 made of metal, on which a molded part 22 made of an electrically insulating material, preferably plastic, is attached.
  • a vertically continuous channel 23 penetrates both the base plate 21 and the molded part 22.
  • a lower sleeve 24 made of metal is arranged, which surrounds a connecting hose 25 made of plastic. Both are passed through the base plate 21 and protrude beyond the underside thereof.
  • a pipette tip 26 is plugged onto the lower sleeve 24 with a tube 27 narrowing downwards, which projects into the channel 23 within the connecting hose 25, and a conical sleeve 28, which bears against the outside of the lower sleeve 24 and one at the lower end of a recess the sleeve 28, which receives the lower part of the lower sleeve 24, arranged ring 29, which consists of electrically conductive material.
  • the tube 27 consists of electrically conductive plastic or metal and is electrically conductively connected to the base plate 21 via the ring 29 and the lower sleeve 24.
  • Above the lower sleeve 24 is the connecting hose 25 from surrounded by an upper sleeve 30 made of metal, the lower part of which lies in the channel 23 and which projects into the hollow profile 12.
  • the upper sleeve 30 is spaced from the lower sleeve 24 and is electrically isolated. It is electrically conductively connected via a tab 31 to an external contact of a plug 32, which is inserted into a recess in the molded part 22 which extends as far as the base plate 21.
  • a shielded cable 33 extends from the plug 32, which leads to a circuit 34 (FIGS. 1, 2), and whose grounded shield is electrically conductively connected to the upper sleeve 30 via the external contact of the plug 32 and the tab 31, while its Soul with the base plate 21 and via this with the tube 27 of the pipette tip 26 is connected.
  • the pipette head 20 further comprises a slider 35 which can be displaced vertically to a limited extent relative to the base plate 21 and the molded part 22 and which is acted upon by a downward force by a spiral spring 36 which is supported on the tab 31 and presses against a transverse bolt 37. However, it is held in place by the attached pipette tip 26.
  • Control of the pipetting device can be used. For example, the lowering of the corresponding pipette tip can be stopped and the liquid can be sucked in. If no pipette tip is plugged on, the slide 35 is in a lower position (not shown), in which the transverse bolt 37 rests on the base plate 21, so that a short circuit is produced by the spiral spring 36, which is also from the circuit 33 is registered.
  • the slide 35 At the upper end of the hollow profile 12 is connected to an elastic sleeve 38, which is connected to the hollow profile 12 by means of a connecting sleeve 39, the lower part of which is slipped onto the hollow profile 12 and over the narrower upper part of the sleeve 38 a vertical starting direction is stamped.
  • the envelope tube 38 is guided to the carrier 1, more precisely to the top of the C-profile 3, where its opposite end is fixed.
  • the fastening can also be designed in such a way that it impresses an approximately perpendicular starting direction on the adjoining section of the casing tube 38.
  • the connecting hose 25 runs through the channel 11 in the hollow profile 12 and further inside the casing tube 38 and an opening in the C-profile 3 inside the same, where it is guided to the end of the carrier 1.
  • the cable 33 originating from the plug 32 is also pulled through the opening 19 in the lower end of the hollow profile 12 into the channel 11, through which it also reaches the upper end of the hollow profile 12 and further inside the casing tube 38 into the interior of the C- Profile 3 runs, from where it is drawn through further openings in the same and in the shell 2a to the circuit 34.
  • the length and the rigidity of the enveloping tube 38 are each selected such that it forms a relatively tight arc at every position of the pipette from which it originates, so that it does not hinder the movements of the pipette, but also does not itself carry out any uncontrolled movements .
  • the envelope tube 38 is preferably made of plastic. It can be provided with an embossed helical structure and / or be reinforced by a braid.
  • the enveloping tubes 38 ensure secure and controlled guidance, above all of the cables 33, but also of the connecting tubes 25, which should therefore be relatively soft and flexible, and protect the pipetting device from malfunctions which would otherwise result from cables getting caught on others Parts could be caused.
  • an enveloping tube other solutions are also conceivable, e.g. B.
  • a differently designed support structure or a differently designed support member such as an elastic flap made of plastic, on which the cable and the connecting hose at one point or preferably on are fixed in several places or continuously.
  • the connecting hose itself to be relatively rigid and to use it to support the softer, more flexible cable.
  • the cable can, for example, be attached to the connecting hose with clips and / or wound around it.
  • the cable does not extend from the pipette head 20, but from the upper end of the pipette housing. This significantly reduces the risk of undesirable effects on the movements of other parts of the pipetting device.

Abstract

Selon l'invention, plusieurs pipettes (5) sont placées de manière à pouvoir coulisser longitudinalement et monter et descendre dans un support oblong (1). Chaque extrémité (26) des pipettes est électroconductrice de sorte que, lorsqu'elle plonge dans un liquide, une variation de capacité se produit entre ladite extrémité et des pièces reliées de manière électroconductrices à cette dernière ainsi que d'autres pièces mises à la terre, ladite variation étant enregistrée pour la détection du liquide. A cet effet, l'extrémité de pipette (26) est reliée par un câble flexible (33) à un circuit (34) installé dans le support (1). Afin d'éviter que le câble (33) ne se prenne dans des pièces mobiles, il est guidé, ainsi que le tuyau flexible de raccordement (25) de la pipette (5), dans un canal d'un boîtier de pipette se présentant sous la forme d'un profilé creux (12) jusqu'à son extrémité supérieure puis de préférence à travers l'intérieur d'un tuyau de gainage (38) plus rigide, se présentant sous la forme d'un élément de soutien, vers un profilé (3) en forme de C du support (1).
EP02734828A 2001-01-25 2002-01-10 Dispositif de pipettage Withdrawn EP1354211A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
CH1292001 2001-01-25
CH129012001 2001-01-25
PCT/CH2002/000013 WO2002059626A1 (fr) 2001-01-25 2002-01-10 Dispositif de pipettage

Publications (1)

Publication Number Publication Date
EP1354211A1 true EP1354211A1 (fr) 2003-10-22

Family

ID=4387602

Family Applications (1)

Application Number Title Priority Date Filing Date
EP02734828A Withdrawn EP1354211A1 (fr) 2001-01-25 2002-01-10 Dispositif de pipettage

Country Status (3)

Country Link
US (1) US20040076550A1 (fr)
EP (1) EP1354211A1 (fr)
WO (1) WO2002059626A1 (fr)

Cited By (1)

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Publication number Priority date Publication date Assignee Title
US9459272B2 (en) 2010-03-22 2016-10-04 BioNex Solutions, Inc. Transfer or interrogation of materials by carrier and receiving devices moving independently and simultaneously on multiple axes

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US20020106308A1 (en) * 2001-02-02 2002-08-08 Zweifel Ronald A. Microdrop dispensing apparatus
EP1477815B1 (fr) * 2003-05-14 2006-07-26 Tecan Trading AG Dispositif pour s'approcher précisément des puits d'une microplaque
DE602004000441T2 (de) * 2004-01-15 2006-08-10 Agilent Technologies, Inc. (n.d.Ges.d.Staates Delaware), Palo Alto Positionierung für eine Flüssigkeitsübertragung
DE102005060862B3 (de) * 2005-12-20 2007-06-28 Stratec Biomedical Systems Ag Verfahren und Vorrichtung zur Beurteilung eines Dosiervorgangs
US7988934B2 (en) * 2006-04-28 2011-08-02 Tecan Trading Ag Carrier for positioning objects in relation to laboratory articles
CH699374B1 (de) * 2006-04-28 2010-02-26 Tecan Trading Ag Träger zum Positionieren von Objekten gegenüber Laborartikeln.
DE102006034245C5 (de) * 2006-07-21 2014-05-28 Stratec Biomedical Systems Ag Positioniereinrichtung zur Positionierung von Pipetten
WO2009023448A1 (fr) 2007-08-13 2009-02-19 Polyone Corporation Mélanges de polyoléfines électriquement conducteurs
US8136383B2 (en) 2007-08-28 2012-03-20 Westerngeco L.L.C. Calibrating an accelerometer
FR2920675B1 (fr) * 2007-09-10 2010-12-03 Gilson Sas Systeme de pipetage multicanaux comprenant un porte-pistons a guidage ameliore
DE202008006338U1 (de) 2008-05-12 2009-03-26 Matthias, Torsten, Dr. Analysegerät
DE102008022835B3 (de) 2008-05-12 2009-10-22 Torsten Dr. Matthias Analysegerät
WO2011157303A1 (fr) 2010-06-18 2011-12-22 Tecan Trading Ag Cisaille d'étalement
JP5846773B2 (ja) 2010-06-29 2016-01-20 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ サンプルの分配
EP2410342B1 (fr) 2010-06-29 2020-02-19 F.Hoffmann-La Roche Ag Dispositif de pipetage avec pointes de pipette mobiles indépendamment
US10591501B2 (en) 2011-10-31 2020-03-17 Torsten Matthias Automatic structure determination
DE102011117273A1 (de) 2011-10-31 2013-05-02 Torsten Matthias Automatische Strukturbestimmung
CH708139A2 (de) * 2013-06-06 2014-12-15 Tecan Trading Ag Pipettiervorrichtung.

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Publication number Priority date Publication date Assignee Title
US9459272B2 (en) 2010-03-22 2016-10-04 BioNex Solutions, Inc. Transfer or interrogation of materials by carrier and receiving devices moving independently and simultaneously on multiple axes
US9823264B2 (en) 2010-03-22 2017-11-21 BioNex Solutions, Inc. Transfer or interrogation of materials by carrier and receiving devices moving independently and simultaneously on multiple axes

Also Published As

Publication number Publication date
US20040076550A1 (en) 2004-04-22
WO2002059626A1 (fr) 2002-08-01

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