EP1183098A1 - Reacteur contenant un tube siphon - Google Patents

Reacteur contenant un tube siphon

Info

Publication number
EP1183098A1
EP1183098A1 EP00914180A EP00914180A EP1183098A1 EP 1183098 A1 EP1183098 A1 EP 1183098A1 EP 00914180 A EP00914180 A EP 00914180A EP 00914180 A EP00914180 A EP 00914180A EP 1183098 A1 EP1183098 A1 EP 1183098A1
Authority
EP
European Patent Office
Prior art keywords
reaction vessel
reaction
housing
vessel according
siphon
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP00914180A
Other languages
German (de)
English (en)
Inventor
Johann Schwartz
Heinz STADTMÜLLER
Walter Hudler
Wolfgang JÖRG
Martin Schmidt
Gerhard Steiner
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Boehringer Ingelheim Pharma GmbH and Co KG
Original Assignee
Boehringer Ingelheim Pharma GmbH and Co KG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Boehringer Ingelheim Pharma GmbH and Co KG filed Critical Boehringer Ingelheim Pharma GmbH and Co KG
Publication of EP1183098A1 publication Critical patent/EP1183098A1/fr
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5025—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures for parallel transport of multiple samples
    • B01L3/50255—Multi-well filtration
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00281—Individual reactor vessels
    • B01J2219/00283—Reactor vessels with top opening
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00279—Features relating to reactor vessels
    • B01J2219/00281—Individual reactor vessels
    • B01J2219/00286—Reactor vessels with top and bottom openings
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J2219/00274—Sequential or parallel reactions; Apparatus and devices for combinatorial chemistry or for making arrays; Chemical library technology
    • B01J2219/00277—Apparatus
    • B01J2219/00351—Means for dispensing and evacuation of reagents
    • B01J2219/00423—Means for dispensing and evacuation of reagents using filtration, e.g. through porous frits
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B60/00—Apparatus specially adapted for use in combinatorial chemistry or with libraries
    • C40B60/14—Apparatus specially adapted for use in combinatorial chemistry or with libraries for creating libraries

Definitions

  • the invention relates to a reaction vessel for holding a reaction liquid which can be used together with other reaction vessels in a reaction block for carrying out solid-phase syntheses and whose reaction liquid can be fed to a microtiter plate via a siphon outlet.
  • Such a reaction vessel is known from US Pat. No. 5,770,157 A.
  • a tube piece is inserted into the outlet opening of this reaction vessel, which is firmly connected at one end by melting to the bottom of the reaction vessel and the other end is to be suspended in a drainage tube so that the reaction liquid is conveyed into it can.
  • the drainage tubes are arranged at a lateral distance from each other next to a reaction vessel in a reaction block, so that the tube piece is to be bent 180 ° both in the area where it merges with the bottom and in its other end area.
  • the siphon outlet formed by the hose piece is accordingly arranged laterally next to the reaction vessel outside of it.
  • the arrangement of the piece of tubing forming the siphon outlet takes up a considerable amount of space next to the reaction vessel in the reaction block, the reaction block is thus almost completely filled by the reaction vessels and tube pieces, so that uniform heating or cooling of the reaction vessels by rinsing is almost impossible.
  • the known reaction vessels are therefore unsuitable for use in a reaction block.
  • the object of the invention is therefore to create a reaction vessel for receiving a reaction liquid of the aforementioned type, which is not only designed in a structurally simple manner and is therefore economical to produce, but which can also be almost completely ruled out that leaks occur. Rather, it should be achieved that an additional processing to form a siphon outlet is not required and that considerable space can be saved with the same capacity of the reaction vessel, so that in a reaction block larger free spaces for the supply of liquids and gases to the in one Reaction block used to heat or cool evenly used reaction vessels. In addition, a versatile usability with low susceptibility to malfunction and simple handling should be guaranteed.
  • a reaction vessel for receiving a reaction liquid of the type mentioned at the outset in that a siphon tube connected to the outlet thereof is preferably centrally located in the reaction vessel and a riser tube is located at a radial distance therefrom End region is closed, are arranged and that the riser communicates in the lower region of the reaction vessel with its receiving space and is connected to the siphon tube in the upper region.
  • the siphon tube in one piece with a housing forming the receiving space of the reaction vessel and preferably to spray it onto the bottom thereof and to insert the riser pipe into the housing at an axial distance from it, with the bottom of the riser pipe being held in the housing in the housing
  • Edge area can be provided with a preferably circumferential extension, in particular in the form of a shoulder, on which the riser pipe is supported with a molded collar, and the collar should preferably have symmetrically arranged through openings.
  • a filter insert in particular in the form of a frit, in the transition area between the receiving space of the housing and the riser tube, which can be supported on the collar of the riser tube, and the riser tube in the upper edge area with one in the receiving area of the Ge -
  • the throttle for. B. should be assigned in the form of a frit used in the interior of the riser.
  • reaction vessel is designed to hold a reaction liquid according to the invention, it is not only possible to assemble it in an extremely simple manner, but it is also almost impossible for the individual components of the reaction vessel to be damaged.
  • the riser tube has to be put over the siphon tube formed on the housing of the reaction vessel in order to create a reaction vessel which can be used in a very versatile manner over a long period of time and without operational disturbances being feared.
  • the siphon tube is arranged inside the housing of the reaction vessel and thus space is saved, that when the reaction vessel is inserted into a reaction block there are large free spaces in which to cool and heat the reaction liquids filled in the reaction vessels the open spaces are flushed with liquids or gases, can be used. A uniform influencing of the individual reaction vessels is therefore ensured, so that there is a versatile usability with simple handling.
  • the drawing shows an exemplary embodiment of the reaction vessel designed according to the invention for holding a reaction liquid, which is explained in detail below:
  • the designated 1 in an axial section reaction vessel serves to receive reaction liquids 2 in order to be able to carry out solid-phase syntheses in a reaction block, and essentially consists of a cup-shaped housing 11, a centrally formed on the bottom 13 in the receiving space 12 of the Housing 11 projecting siphon tube 16 and a riser tube 21 slipped over it and arranged at a lateral distance from it, which is supported on the bottom 13 of the housing 11 and communicates with the interior 12 thereof.
  • the riser pipe 21 an annular flange 23 is formed, and on the bottom 13 of the housing 11 a peripheral projecting extension 14 is provided in the edge region, on which the flange 23 rests.
  • the riser pipe 21 or its flange 23 is thus supported at a distance from the bottom 13. And since the flange 23 is provided with openings 24 arranged in a uniformly distributed manner, the reaction liquid 2 can flow from the interior 12 of the housing 11 into the annular space 22 formed between the siphon tube 16 and the riser tube 21. In addition, a filter insert 25 in the form of an annular frit is placed on the flange 23, particles in the reaction liquid are thus retained and do not get into the annular space 22.
  • reaction vessels 1 After a plurality of reaction vessels 1 have been inserted into a reaction block (not shown) and filled with the reaction liquid 2, these are sealed airtight together with the aid of a plate 20. If appropriate, the reaction vessels 1 are then heated or cooled in order to influence the solid phase synthesis. When this process is complete, the reaction liquid has to be transferred to a microplate. This is done in such a way that the reaction liquid 2 located in the receiving space 12 of the housing 11 is acted upon by a pressurized gas which can be supplied via a bore 18 machined into the upper region of the housing 12.
  • reaction liquid 102 rises in the annular space 22 because the riser tube 21 is closed in the upper end region and flows into the interior 17 of the siphon tube 16, which is connected to an outlet connection 15 formed centrally on the outside of the base 13 is.
  • the reaction liquid 2 can accordingly be filled into the respective receiving space of a microtiter plate.
  • a frit 27 is also arranged in front of the through hole 26, which acts as a throttle, so that only a small amount of air can be sucked in.

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Organic Chemistry (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Devices And Processes Conducted In The Presence Of Fluids And Solid Particles (AREA)

Abstract

L'invention concerne un réacteur (1) pouvant être utilisé pour réaliser des synthèses en phase solide dans un bloc de réaction, le liquide de réaction (2) pouvant être acheminé par l'intermédiaire d'une évacuation de siphon d'une plaque de microtitration. Selon l'invention, un tube siphon (16) est placé au niveau de l'évacuation (15) du réacteur, et un tube ascendant (21) est placé à une distance radiale dudit tube siphon (16). Ce tube ascendant (21) est fermé au niveau de sa partie supérieure et relié au tube siphon (16). Il communique au niveau de la zone inférieure du réacteur avec une zone de réception (12) de ce dernier. Cette conception permet d'éviter d'avoir recours à un usinage supplémentaire pour former l'évacuation de siphon et empêche l'apparition de pratiquement tout défaut d'étanchéité dans la zone de l'évacuation de siphon.
EP00914180A 1999-04-08 2000-04-04 Reacteur contenant un tube siphon Withdrawn EP1183098A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE1999115811 DE19915811C2 (de) 1999-04-08 1999-04-08 Reaktionsgefäß
DE19915811 1999-04-08
PCT/EP2000/002988 WO2000061279A1 (fr) 1999-04-08 2000-04-04 Reacteur contenant un tube siphon

Publications (1)

Publication Number Publication Date
EP1183098A1 true EP1183098A1 (fr) 2002-03-06

Family

ID=7903862

Family Applications (1)

Application Number Title Priority Date Filing Date
EP00914180A Withdrawn EP1183098A1 (fr) 1999-04-08 2000-04-04 Reacteur contenant un tube siphon

Country Status (7)

Country Link
EP (1) EP1183098A1 (fr)
JP (1) JP2002540931A (fr)
AU (1) AU3558800A (fr)
CA (1) CA2372796A1 (fr)
DE (1) DE19915811C2 (fr)
MX (1) MXPA01010014A (fr)
WO (1) WO2000061279A1 (fr)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10057195A1 (de) * 2000-11-17 2002-05-23 Robert Loesel Reaktionsgefäß mit Membranboden
EP1291074A1 (fr) * 2001-09-07 2003-03-12 F. Hoffmann-La Roche Ag Bloc de réaction pour la chimie de synthèse en parallèle et récipient pour cela
EP1291075B1 (fr) * 2001-09-07 2007-04-11 F. Hoffmann-La Roche Ag Bloc de réaction pour la chimie de synthèse en parallèle et récipient pour cela

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE940110L (en) * 1989-03-23 1990-09-23 Bunce Roger A Liquid transfer devices
US5516491A (en) * 1994-07-28 1996-05-14 Merck & Co., Inc. Disposable reactor vessel
SE503529C2 (sv) * 1994-10-17 1996-07-01 System Teeg Ab Handfatsventil med integrerat vattenlås
US5609826A (en) * 1995-04-17 1997-03-11 Ontogen Corporation Methods and apparatus for the generation of chemical libraries
US5746982A (en) * 1996-02-29 1998-05-05 Advanced Chemtech, Inc. Apparatus for automated synthesis of chemical compounds
AU2939497A (en) * 1996-05-10 1998-01-05 Alanex Corporation Simultaneous chemical reaction apparatus

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO0061279A1 *

Also Published As

Publication number Publication date
MXPA01010014A (es) 2002-04-24
CA2372796A1 (fr) 2000-10-19
JP2002540931A (ja) 2002-12-03
DE19915811C2 (de) 2001-05-03
DE19915811A1 (de) 2000-10-19
AU3558800A (en) 2000-11-14
WO2000061279A1 (fr) 2000-10-19

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