EP0969917A1 - Verfahren und vorrichtung zum produzieren von suspensionen aus partikeln und flüssigkeit - Google Patents

Verfahren und vorrichtung zum produzieren von suspensionen aus partikeln und flüssigkeit

Info

Publication number
EP0969917A1
EP0969917A1 EP98917266A EP98917266A EP0969917A1 EP 0969917 A1 EP0969917 A1 EP 0969917A1 EP 98917266 A EP98917266 A EP 98917266A EP 98917266 A EP98917266 A EP 98917266A EP 0969917 A1 EP0969917 A1 EP 0969917A1
Authority
EP
European Patent Office
Prior art keywords
liquid
particles
container
volume
deposit
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP98917266A
Other languages
English (en)
French (fr)
Other versions
EP0969917B1 (de
Inventor
Bruno Colin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Original Assignee
Biomerieux SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux SA filed Critical Biomerieux SA
Publication of EP0969917A1 publication Critical patent/EP0969917A1/de
Application granted granted Critical
Publication of EP0969917B1 publication Critical patent/EP0969917B1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/45—Magnetic mixers; Mixers with magnetically driven stirrers
    • B01F33/451—Magnetic mixers; Mixers with magnetically driven stirrers wherein the mixture is directly exposed to an electromagnetic field without use of a stirrer, e.g. for material comprising ferromagnetic particles or for molten metal

Definitions

  • the present invention relates to the suspension of particles of a solid, in a predetermined volume of liquid, the particles and the volume of liquid being contained together inside a container itself delimited by a wall.
  • the invention is concerned with the suspension, or resuspension of particles, forming a deposit adhering to the internal face of the wall of the container, in the form of a continuous film or of aggregates, and bringing together at least a portion of these particles in the agglomerated state, and this in a predetermined volume of the liquid, contained inside said container.
  • particles / liquid defined above is encountered in particular during certain biological analyzes, using particles, of size at most equal to 10 ⁇ m, and preferably between 0.01 ⁇ m and 10 / / m, and for example between 1 and 5 // m, these particles comprising a magnetic substrate on which is fixed at least one analyte, or at least one reagent, and being dispersed in a continuous liquid phase, for example an aqueous phase .
  • these particles under the effect of a magnetic field, from the liquid phase, leads in part to their accumulation and agglomeration in the form of a deposit, continuous or discontinuous, adhering to the internal face of the container wall.
  • the method described above is specific to magnetic particles, and can only be implemented with such particles, because it is the conjunction of the rotational movement of said particles, generated by the rotating magnetic field, and the translational movement relative, alternative, between said field and the container, which detaches and disperses any deposit of magnetic particles liable to agglomerate on the internal face of the container.
  • the present invention therefore relates to a process for suspending particles, from the physical state described or defined above, independent of the nature of the particles, and purely mechanical or hydrodynamic, without introducing into the container an additional quantity of liquid.
  • the solution according to the invention also provides another advantage.
  • the maximum height of the reflux flow obtained according to the present invention mechanically makes it possible to lick and loosen agglomerated particles on the internal face of the container, above the normal level.
  • FIG. 1 shows schematically a container, in the physical state of its different phases before the application of the method according to the invention
  • FIG. 3 shows schematically particles as used by the invention.
  • - Figures 4, 5 and 6 respectively represent the influence of agitation on the signal values obtained for different types of particles (Fig. 4: Estapor particles, Fig. 5: Dynal particles, Fig. 6: Seradyn particles) .
  • the measurement of the light signal is expressed in RLU ("Relative Light Unit").
  • RLU Relative Light Unit
  • the implementation of the present invention is independent of the magnetic nature or not of the particles.
  • a treatment which consists in applying a magnetic field for example at the level of the lateral walls of the container 1 2 the particles are found, either completely gathered and agglomerated in the form of a deposit 4 adhering to the internal face 3a of the wall 3, that is to say partly dispersed within the volume of liquid and partly gathered and agglomerated in the form of a deposit 4 as described below. above.
  • the particles 1 are, partly dispersed within the volume of liquid, and remain partly gathered and agglomerated in the form of a deposit 4 (continuous film or aggregates) adhering to the internal face 3a of the wall 3.
  • a means 9 for securing the container and a means 10 for alternately moving the securing means 5, in a reference direction 6, for example vertical.
  • This alternative displacement means can consist of a simple eccentric cooperating with the means 9 for securing the container.
  • the container 1 2 is subjected to an alternating movement 5 in the reference direction 6, with a relatively large frequency, at least equal to 10 Hz, and one obtains or observes, within the predetermined volume of liquid, a flow / reflux 7 against the wall 3 of the container, on either side of the reference direction 6, breaking the deposit 4, and dispersing the particles 1 towards the center volume of liquid.
  • a relatively large frequency at least equal to 10 Hz
  • the predetermined volume 2 of liquid contains, homogeneously and completely dispersed, the particles 1, so that the analysis process can be continued.
  • its amplitude is between 1 mm and 15 mm, preferably between 1 mm and 8 mm, and for example between 3 mm and 6 mm,
  • the reciprocating movement has a frequency preferably between 1 5 Hz and 60 Hz, for example between 1 5 Hz and 30 Hz, for example, the preferred frequency is 1 6 Hz,
  • the direction of the reciprocating movement is preferably vertical, that is to say perpendicular to the horizontal interface 2a of the predetermined volume of liquid; but the direction of the reciprocating movement optionally includes a vertical main component and a horizontal secondary component.
  • Example: The present invention has been implemented in the context of a quantitative analysis of a pathogenic microorganism, namely Toxoplasma M (analyte), with magnetic particles coated with a ligand (antibody) specific for said microorganism.
  • the particles are incubated in an analysis cuvette (container), with a sample or inoculum containing said microorganism. Then the particles which have reacted with said microorganism are separated by means of a magnetic field, bringing them together and fixing them against the wall of the bowl. The bowl is then emptied, then washed. A predetermined volume of a reagent (antibody labeled, for example by an enzyme such as alkaline phosphatase, directed against the analyte is then introduced and incubated in the cuvette, after removal of the magnetic field. After incubation and separation of the elements n ' having not reacted, a luminescent detection reagent is introduced into the cuvette and the light signal corresponding to the reagent having reacted with the particles is measured.
  • a reagent antibody labeled, for example by an enzyme such as alkaline phosphatase
  • the resuspension of the particles according to the method and the device of the invention facilitates the contact of the particles with the reactive entities (analyte and / or detection reagent), which results in an increase in the measured signal, for example the signal luminous.
  • the implementation of the resuspension or resuspension according to the invention makes it possible to increase the measurement light signal by at least 60%, or even in certain cases of the order of 100%, according to the magnetic particles used, for example under the commercial references Estapor NH2-gluta, Dynal M280, and Seradyn. The results with and without stirring are presented below, respectively with each type of particles.
EP98917266A 1997-03-27 1998-03-27 Verfahren und vorrichtung zum produzieren von suspensionen aus partikeln und flüssigkeit Expired - Lifetime EP0969917B1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR9704017 1997-03-27
FR9704017A FR2761277B1 (fr) 1997-03-27 1997-03-27 Procede et dispositif de mise en suspension de particules d'un solide dans un liquide
PCT/FR1998/000639 WO1998043730A1 (fr) 1997-03-27 1998-03-27 Procede et dispositif de mise en suspension de particules d'un solide dans un liquide

Publications (2)

Publication Number Publication Date
EP0969917A1 true EP0969917A1 (de) 2000-01-12
EP0969917B1 EP0969917B1 (de) 2002-01-16

Family

ID=9505443

Family Applications (1)

Application Number Title Priority Date Filing Date
EP98917266A Expired - Lifetime EP0969917B1 (de) 1997-03-27 1998-03-27 Verfahren und vorrichtung zum produzieren von suspensionen aus partikeln und flüssigkeit

Country Status (10)

Country Link
US (1) US6390660B1 (de)
EP (1) EP0969917B1 (de)
JP (1) JP2001517149A (de)
AT (1) ATE211946T1 (de)
AU (1) AU7053198A (de)
CA (1) CA2283687A1 (de)
DE (1) DE69803166T2 (de)
ES (1) ES2171017T3 (de)
FR (1) FR2761277B1 (de)
WO (1) WO1998043730A1 (de)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6808304B2 (en) * 2002-08-27 2004-10-26 Dade Behring Inc. Method for mixing liquid samples using a linear oscillation stroke
US7258480B2 (en) * 2005-01-10 2007-08-21 Dade Behring Inc. Apparatus for mixing liquid samples using a two dimensional stirring pattern
DE502007004200D1 (de) * 2007-08-14 2010-08-05 Qiagen Gmbh Verfahren zum Suspendieren oder Resuspendieren von Partikeln in einer Lösung sowie daran angepasste Vorrichtung
WO2018161204A1 (zh) * 2017-03-06 2018-09-13 东北大学 一种利用间歇共振的混合物封闭搅拌器及方法

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US1417219A (en) * 1921-08-22 1922-05-23 Thomas H Warren Autohemic therapy potentizer
DE560885C (de) * 1929-02-23 1932-10-08 Martin Stuehler Verfahren und Vorrichtung zum Herstellen von Betonkoerpern o. dgl. durch Ruetteln
US1999671A (en) * 1934-06-30 1935-04-30 Hubert Van Bree Amalgam mixer
US2198637A (en) * 1938-12-24 1940-04-30 Submarine Signal Co Apparatus for treating metals
US2561027A (en) * 1949-06-11 1951-07-17 Velsicol Corp Variable frequency vibrating device
US2766881A (en) * 1951-03-26 1956-10-16 Research Corp Acoustic separatory methods and apparatus
DE1457328A1 (de) * 1965-11-22 1969-10-23 Abe Hershler Verfahren und Vorrichtung zum Ruehren und Bewegen von stroemungsfaehigen Medien
US3498384A (en) * 1966-11-08 1970-03-03 Chyugoku Kogyo Kk Vibratory impact device
US3587193A (en) * 1968-12-04 1971-06-28 E R Lewis Rock polisher
GB1330975A (en) * 1971-11-05 1973-09-19 N I Kt I Eamliro Vannogi Khim Apparatus for carrying out physical and chamical processes in a vortex bed of ferromagnetic particles
US3764116A (en) * 1972-02-28 1973-10-09 Branson Instr Ultrasonic treatment apparatus
US4085955A (en) * 1976-11-22 1978-04-25 Salle Donald A Pen shaking device
DE7817065U1 (de) * 1978-06-07 1978-09-28 Kerscher, Wolfgang, Ing.(Grad.), 8400 Regensburg Schuettelmaschine, insbesondere fuer tuscheschreibgeraete
DE3046157A1 (de) * 1980-12-06 1982-07-22 Deutsche Wurlitzer GmbH, 4971 Hüllhorst Getraenke-selbstverkaufs-automat
DE3233926A1 (de) * 1981-09-14 1983-04-28 Fuji Electric Corporate Research and Development, Ltd., Yokosuka, Kanagawa Zerkleinerungs-, misch- oder ruehrvorrichtung
JPS58158182A (ja) * 1982-03-15 1983-09-20 Olympus Optical Co Ltd 培養細胞の剥離方法およびその装置
JPS6058235A (ja) * 1983-09-07 1985-04-04 Toshiba Corp 撹拌方法および撹拌装置
US4610546A (en) * 1984-12-31 1986-09-09 Technicon Instruments Corporation Apparatus and method for self-resonant vibrational mixing
FR2600137B1 (fr) * 1986-06-16 1990-06-22 Pechiney Aluminium Suspension pneumatique des vibrotasseuses utilisees en particulier pour la production de blocs carbones
US4841848A (en) * 1987-02-17 1989-06-27 F. Korbel And Bros. Method and apparatus for riddling wine in bottles
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Also Published As

Publication number Publication date
JP2001517149A (ja) 2001-10-02
DE69803166T2 (de) 2002-08-29
DE69803166D1 (de) 2002-02-21
US6390660B1 (en) 2002-05-21
FR2761277B1 (fr) 2000-01-28
ATE211946T1 (de) 2002-02-15
FR2761277A1 (fr) 1998-10-02
WO1998043730A1 (fr) 1998-10-08
CA2283687A1 (fr) 1998-10-08
ES2171017T3 (es) 2002-08-16
AU7053198A (en) 1998-10-22
EP0969917B1 (de) 2002-01-16

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