Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
  • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
  • C07K14/52—Cytokines; Lymphokines; Interferons
  • C07K14/53—Colony-stimulating factor [CSF]
  • C07K14/535—Granulocyte CSF; Granulocyte-macrophage CSF
• • B—PERFORMING OPERATIONS; TRANSPORTING
  • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
  • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
  • B01J39/00—Cation exchange; Use of material as cation exchangers; Treatment of material for improving the cation exchange properties
  • B01J39/26—Cation exchangers for chromatographic processes
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07K—PEPTIDES
  • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
  • C07K1/14—Extraction; Separation; Purification
  • C07K1/16—Extraction; Separation; Purification by chromatography
  • C07K1/18—Ion-exchange chromatography

Definitions

• Ion-exchange chromatography is a conventional chromatographic technique, inherently mild, low cost, large capacity and readily scalable. However, to date it has not been a useful technique for separating closely related impurities from proteins, especially rDNA proteins.
• rDNA protein is human recombinant GM-CSF.
• Complementary DNAs (cDNAs) for GM- CSF factors which support growth and development of granulocytes and macrophages in the blood, have recently been cloned and sequenced by a number of laboratories.
• non-recombinant GM-CSF has been purified from culture supernatants of the Mo cell line (described in U.S. Patent 4,438,032), and the first sixteen amino acids from the N-terminus have been sequenced, Gasson et al. , Science, Vol. 226, pgs. 1339-1342 (1984).
• GM-CSF GM-CSF
• nucleotide sequence and amino acid sequence heterogeneity have been observed at the amino acid level.
• threonine and isoleucine have been observed at position 100 with respect to the N- terminal alanine, suggesting that several allelic forms, or polymorphs, of GM-CSF may exist within human populations.
• a variety of methods are now available for de novo preparation and cloning of cDNAs, such as cDNAs for GM-CSF, and for the construction of cDNA libraries.
• total mRNA is extracted from cells (e.g., a nontransformed human T cell source) producing poly- peptides exhibiting the desired activity.
• the double- stranded cDNAs can be constructed from this total mRNA by using primer-initiated reverse transcription to make first the complement of each mRNA sequence, and then by priming for second strand synthesis. Subsequently, the cDNAs can be cloned by joining them to suitable plasmid or bacteriophage vectors through complementary homo- polymeric tails or cohesive ends created with linker segments containing appropriate restriction sites and then transforming a suitable host.
• suitable plasmid or bacteriophage vectors through complementary homo- polymeric tails or cohesive ends created with linker segments containing appropriate restriction sites and then transforming a suitable host.
• a wide range of expression systems i.e., host-expression vector combinations
• Possible types of host cells include, but are not limited to, bacterial, yeast, insect, mammalian and the like.
• Various methods have been disclosed for extracting the GM-CSF from the host cells and subsequently purifying it, but GM-CSF is not always adequately purified in
• the present invention is directed at a method for separating a crude protein into a relatively pure protein fraction and an impure protein fraction characterized by contacting the crude protein with an ion exchange resin at a pH such that the pure protein and the impurity are oppositely charged, whereby one of the fractions is selectively bound to the ion exchange resin.
• the ion exchange resin preferably is a strong ion exchange resin and the pure protein fraction preferably is bound to the ion exchange resin.
• the fraction bound to the ion exchange resin preferably is eluted.
• the pH of the crude protein preferably is adjusted to a pH within the range of isoelectric points of the protein fractions to be separated such that there is an amplified net charge difference between the fractions.
• the present invention also is directed at a method for determining the pH at which to separate a crude protein into a relatively pure protein fraction and an impure protein fraction characterized by determining the isoelectric points of the pure protein to be recovered and the impurity to be removed and determining the pH within the range of isoelectric points such that the pure protein and the impurity are oppositely charged and an amplified difference between said charges exists.
• the isoelectric points of the protein fractions preferably are determined by computer simulation or by using isoelectric focusing gels.
• GM-CSF granulocyte macrophage colony stimulating factor
• protein is purified by sequentially contacting the protein with:
• a cation exchange resin preferably having a sulfonate functionality attached to a cross-linked dextran, cellulose, agarose or acrylic support
• the present invention relates to a high resolution ion-exchange chromatographic separation techique for purifying crude proteins, particularly crude rDNA proteins.
• chromatography is carried out near the isoelectric points of the pure protein sought to be recovered and the impurity sought to be removed, preferably at a pH where an amplified (e.g. the maximum) difference between charges on the pure protein and impurity exists, and where the molecules are oppositely charged (polarized).
• This amplified net charge difference which occurs only near the isoelectric point, can be used to select appropriate ion exchange chromatography conditions, resulting in selective binding to ion exchange resin in the near absence of charges.
• this technique is hereafter referred to as Delta Isoelectric Point (DIP) chromatography.
• DIP Delta Isoelectric Point
• rDNA impurities closely related to a desired rDNA protein are difficult to remove from crude protein by gel filtration chromatography, ion exchange chromatography, hydrophobic interaction chromatography, reversed-phase high performance liquid chromatography, metal chelating affinity chromatography as well as other conventional types of chromatography.
• These closely related impurities usually are proteins with a molecular weight and charge distribution close to the protein product of interest, usually missing one (or more) charged amino acids. Examples are impurities that are proteolytic degradation products formed by nicking a peptide terminus that is accessible to proteolysis as well as to chromatographic interaction. Such impurities are considered inseparable by conventional ion exchange chromatography.
• the DIP chromatography technique differs from conventional ion exchange chromatography in a number of ways. DIP is performed at a pH where proteins to be separated possess very small net electrical charges. This is achieved by loading the ion exchange resin at a pH that is only fractional pH units away from the isoelectric point (pi) of the desired protein and its closely related impurities. This is in contrast to conventional ion exchange chromatography, which is performed at pH's where proteins of interest possess strong electrical charges, and wherein loading is carried out about 1-2 whole pH units away from the pi, an order of magnitude greater than in DIP chromatography.
• the pH at which to perform DIP is predicted by computer simulations of charge density vs. pH for the protein and related impurities, or by analytical techniques such as the use of isoelectric focusing gels. In conventional ion exchange chromatography, loading pH is determined by gross net charge on the desired protein, and loading conditions are optimized empirically, if at all. Tedious trial and error experimentation is needed to determine elution conditions.
• the loading pH is selected so that it lies between the pi of the closely related impurity and that of the desired protein, and therefore, the desired protein attains an opposite electrical charge to the polarized impurity.
• a high ionic strength environment is imposed to reduce protein-protein interactions during loading, and a strong ion exchanger is used.
• a strong ion exchanger is used in conventional ion exchange technology, to obtain maximum resolution, weak ion exchange resins with lower ionic strength loading conditions are preferred.
• Computer simulation of charge density vs. pH can be conducted using commercially available software and an appropriate computer (e.g. mainframe, microcomputer or personal computer).
• a VAX computer Digital Corp.
• a software package such as "Polypeptide Analysis System” by Intellegentics, Inc., copyright 1981, 1982, 1983, 1984, 1985 and 1986.
• the primary amino acid sequence is the input and the charge density distribution can be obtained at different pH's.
• pi may be determined by using isoelectric focusing (IEF) gel electrophoresis according to procedures well known to those skilled in the art.
• IEF isoelectric focusing
• DIP chromatography can be applied to proteins, especially recombinant proteins, which have isoelectric points in a pH range wherein the protein is not denatured.
• the isoelectric points of many proteins are known. For example, see the extensive review article by Righetti, et. al. , "Isoelectric Points and Molecular
• GM-CSF GM-CSF
• leukocyte and lymphoblastoid interferons growth hormone, superoxide dismutase and erythropoietin.
• Other proteins include antithrombin III, lactogen, plasminogen, prolactin, urokinase and vitamin B 12 -binding protein.
• GM-CSF is usually purified by a combination of anion exchange on a quaternary aminoethyl column, gel filtration and reversed phase chromatography, which procedure is not effective in removing the ⁇ 4 impurity.
• DIP chromatography has been successful in removing the ⁇ 4 impurity.
• a computer simulation of the intact GM-CSF protein was compared to the computer simulation of the ⁇ 4 impurity, and it was determined that the pi of GM-CSF is 5.24 and the pi of the ⁇ 4 impurity is 4.98.
• a working pH within this range had to be determined at which (a) GM- CSF attained an opposite charge to the ⁇ 4 impurity, and (b) the relative magnitude of the difference between the charges is sufficiently amplified to enable separation.
• a minute charge difference of 1 charge/molecule (1 ch/mol) between the intact GM-CSF and the ⁇ 4 impurity exists over a broad pH range (i.e., at pH 0.5, the respective charges are +16 and +15 and at a pH 11.5, the charges are -11.1 and -12).
• the charge difference between intact GM-CSF and the ⁇ 4 impurity is amplified
• a strong cation exchanger at pH 5 e.g. a column such as a sulfonate functionality attached to a cross-linked dextran, cellulose, agarose or acrylic support (e.g., S- Sepharose, manufactured by Pharmacia, Inc., Piscataway, NJ) is therefore preferred.
• a cation exchange resin column was found to accomplish a one-step removal of the undesirable ⁇ 4 impurity, various low pi impurities, a 20K E. coli impurity not previously removed by other procedures, and a proteolytic factor, thereby stabilizing the final product.
• Performing the cation exchange chromatography raises the purity of the GM-CSF from about 50% to about 90%, with yields of 70-80%.
• the purity of the GM-CSF is raised to about 99%.
• the anion exchange chromatography is a conventional step in the purification of GM-CSF and is performed to remove high pi impurities from the supernatant after the host cells are killed.
• the gel filtration step also conventional, is performed to remove high and low molecular weight impurities. While the following describes a process in which the purified protein fraction is bound to the strong ion exchange resin, it is also contemplated that in certain embodiments of the invention the impure protein fraction may be bound to the strong ion exchange resin.
• other contacting methods such as batch contacting, also are contemplated.
• Protein concentration is determined at each stage by a Coomassie Blue binding assay. If the expected degree of purification is not achieved in any chromatographic procedure, eluted fractions may be re-chromatographed on the same column, or, alternatively, recycled through a previous step or a previous series of steps. This reprocessing can be done on eluted side fractions from a batch or a pool of batches. At any step, concentration may be performed by an ammonium sulfate preciptation, isoelectric point precipitation and/or ultrafiltration. Buffer solutions are made with deionized water (DI), reverse osmosis water
• RO water for injection
• WFI water for injection
• the pH of the crude extract of GM-CSF is adjusted to 5-7.5 with buffer such as 1M Bis-tris (bis [2- hydroxyethyl] imino-tris-[hydroxymethyl]methane) and/or 4N HC1.
• buffer such as 1M Bis-tris (bis [2- hydroxyethyl] imino-tris-[hydroxymethyl]methane) and/or 4N HC1.
• the solution is then clarified by centrifugation and/or filtration.
• the conductivity is adjusted to below 10 millisiemens/centimeter (mS/cm) by dilution with water or addition of a salt solution such as 4N NaCl.
• the batch is applied to a quaternary amine column (i.e., a quaternary amine functionality attached to a cross-linked dextran, cellulose, agarose or acrylic support, such as Q-Sepharose, manufactured by Pharmacia, Inc.) at a loading of not greater than 50 grams of protein per liter of gel. Elution is performed with a gradient in the range of 0-0.4M NaCl or other appropriate salt in a buffer such as 20mM Bis-tris. Appropriate fractions are combined for further processing.
• a quaternary amine column i.e., a quaternary amine functionality attached to a cross-linked dextran, cellulose, agarose or acrylic support, such as Q-Sepharose, manufactured by Pharmacia, Inc.
• Elution is performed with a gradient in the range of 0-0.4M NaCl or other appropriate salt in a buffer such as 20mM Bis-tris. Appropriate fractions are combined for further processing.
• the combined fractions are adjusted to pH 5, which is within the range of isoelectric points of the protein fractions as determined by DIP chromatography, with an acid such as 1M acetic acid or 4N HC1 or a base such as 6N NaOH.
• the conductivity is adjusted to 13 mS/cm with a buffer such as 0.01M acetic acid adjusted to pH 5 with NaOH.
• the solution is filtered through a 0.2 micron filter and charged onto a sulfonate column (i.e., a sulfonate functionality attached to a cross-linked dextran, cellulose, agarose or acrylic support, such as S-Sepharose) at a loading of not greater than 20 grams of protein per liter of column material.
• a sulfonate column i.e., a sulfonate functionality attached to a cross-linked dextran, cellulose, agarose or acrylic support, such as S-Sepharose
• Elution is performed with a solution of a salt such as NaCl at a concentration gradient up to 0.5M in a buffer such as 20mM acetic acid, 0.13M NaCl, pH 5.0 buffer. Appropriate fractions are combined for further processing. This chromatography step is typically repeated.
• a salt such as NaCl
• a buffer such as 20mM acetic acid, 0.13M NaCl, pH 5.0 buffer.
• the ammonium sulfate precipitate is dissolved in a buffer such as lOmM sodium phosphate, 50mM citric acid, pH 6 buffer containing up to 0.35M of a salt such as sodium chloride.
• a buffer such as lOmM sodium phosphate, 50mM citric acid, pH 6 buffer containing up to 0.35M of a salt such as sodium chloride.
• the solution is centrifuged and filtered through a 0.2 micron range filter prior to loading on to a gel filtration column having a fractionation range of from about 5,000 to about 100,000 daltons for proteins, e.g., Sephacryl S-200 HR (manufactured by Pharmacia, Inc.), pre-equilibrated with the same buffer.
• the loading is not greater than 3.5 grams of protein per liter of gel.
• the column is eluted with the same buffer and appropriate fractions are combined.
• the fractions are dialyzed against a buffer such as an lOmM phosphate
• the combined fractions are filtered .through a 0.2 micron or smaller pore size filter and stored at -20°C or below.
• the pH of 180L of crude GM-CSF extract was adjusted to pH 6.0 with 3.6L IM Bis-tris buffer (pH 6.0) and with 2.0L of 4N HC1.
• the solution was clarified by centrifugation using a Sharpies centrifuge at a feed rate of 0.75L/min.
• the supernatant was diluted approximately 1.55 fold with cold deionized water to reach a final conductivity of 5.5 mS/cm.
• a 12L column of Q-Sepharose was equilibrated with 10 column volumes of 20mM Bis-tris buffer at pH 6.0, and 43.6L of extract was applied to the column at a loading of 20 mg protein per ml of resin.
• the column (diameter 25 cm) was washed at a flow rate of 250 l/min. with 120L of equilibration buffer. A linear gradient was established between 78L of 20mM Bis- tris buffer containing 0.03 NaCl at pH 6.0 and 78L of 20mM Bis-tris buffer at pH 6.0 containing 0.32M NaCl. Fractions (1.2L each) were combined based on gel electrophoresis (SDS-PAGE) and the pooled protein (4.9L) was chromatographed in Step 2.
• the combined fractions (4.9L) from Step 1 were adjusted to pH 5 with 49 ml IM acetic acid (pH 5.0) and the conductivity was adjusted to 15 mS/cm with 2L 0.01M acetic acid adjusted to pH 5 with NaOH.
• the solution was filtered through a 0.2 micron filter and then charged to a 0.8L S-Sepharose column previously equilibrated with 20mM acetic acid containing 0.13M NaCl at pH 5 at a loading rate of 4.7 mg/ml column material.
• the column was washed with 2.4L equilibrating buffer, then eluted with a linear gradient established between 5.6L 20mM acetic acid at pH 5 containing 0.13M NaCl and 5.6L 20mM acetic acid at pH 5 containing 0.5M NaCl. The fractions were combined based on gel electrophoresis. This chromatography step was repeated.
• Ammonium sulfate was added to the combined fractions from Step 2 (240L), giving a concentration of 351 g/L (55% saturation). The solution was held at 4°C without mixing for 2 hours, then was centrifuged at 4,500 rpm for 30 min at 4°C to obtain the precipitate.
• the ammonium sulfate pellet was dissolved in 36 ml of 18mM sodium phosphate, 2mM citric acid, pH 7.2 buffer. The solution was centrifuged at 4,500 rpm for 30 min. and the supernatant filtered through a 0.2 micron filter. The filtrate was loaded onto a 1.8L Sephacryl S- 200HR column previously equilibrated with phosphate- citrate pH 7.2 buffer at a loading rate of 0.21 mg/ml gel. The column was eluted with 1.9L of the same pH 7.2 buffer. Fractions were combined based on protein assay. - - Step 5: Filtration

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