EP0232517B1 - Pipette - Google Patents

Pipette Download PDF

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Publication number
EP0232517B1
EP0232517B1 EP86117244A EP86117244A EP0232517B1 EP 0232517 B1 EP0232517 B1 EP 0232517B1 EP 86117244 A EP86117244 A EP 86117244A EP 86117244 A EP86117244 A EP 86117244A EP 0232517 B1 EP0232517 B1 EP 0232517B1
Authority
EP
European Patent Office
Prior art keywords
piston
pipette according
stop
pipette
compression spring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP86117244A
Other languages
German (de)
English (en)
Other versions
EP0232517A3 (en
EP0232517A2 (fr
Inventor
Adolf M. Hirschmann
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Glasgeratebau Hirschmann
Original Assignee
Glasgeratebau Hirschmann
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glasgeratebau Hirschmann filed Critical Glasgeratebau Hirschmann
Priority to AT86117244T priority Critical patent/ATE50518T1/de
Publication of EP0232517A2 publication Critical patent/EP0232517A2/fr
Publication of EP0232517A3 publication Critical patent/EP0232517A3/de
Application granted granted Critical
Publication of EP0232517B1 publication Critical patent/EP0232517B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids

Definitions

  • the invention relates to a pipette with a liquid receiver according to the preamble of claim 1.
  • Such a pipette is known from DE-A-2 159 541.
  • the known pipette has a piston which is guided in a longitudinally displaceable manner in a cylinder, which is connected to a pressure pin protruding from the outer sleeve of the pipette, which is supported on the outer sleeve by a first compression spring and which can be moved beyond the end of the pressure stroke, to move the liquid reamer out of its receptacle. Furthermore, the stroke of the piston is limited by stops.
  • EP-A-0 155 087 shows a pipette with a piston which is guided in a cylinder in a longitudinally displaceable manner and which can be moved beyond the end of the pressure stroke in order to move the liquid receiver out of its receptacle.
  • DE-A-2 851 532 shows a pipette with an elastic bellows, in which the stroke of the bellows is limited by predetermined stops.
  • the bellows is biased in the area in which it has a sufficiently large restoring force between the stops in such a way that it returns to a rest position predetermined by one of the stops without external aids.
  • liquid transducers in particular microcapillaries, are often used for such work, which have to be manually inserted into the pipette.
  • the piston which is longitudinally displaceable in the cylinder, beyond the end of the pressure stroke in order to move the liquid receiver out of its receptacle.
  • the liquid receiver is held in the receptacle by friction, so that it can be moved out of the receptacle by pressure in the longitudinal direction.
  • the liquid force transducer can be removed from the receptacle by hand and separated from the pipette without touching it. Because the liquid receiver is held in a sealing manner in the receptacle, the overpressure required to eject the liquid in the liquid receiver can form during the pressure stroke.
  • the object of the invention is to simplify and improve the handling of the pipette specified at the outset.
  • a second compression spring is provided, which is supported on the one hand on the piston and on the other hand on a stop ring which bears against a stop of the piston during the pressure stroke and subsequently against a stop of the outer sleeve.
  • the force of the second compression spring must be overcome like a pressure point after the end of the pressure stroke when the piston is further depressed, as a result of which the operator can feel the end of the pressure stroke and the start of the ejection phase of the liquid sensor in a reliable manner. This simplifies and improves the handling of the pipette according to the invention.
  • the advantage just described is further increased.
  • the pressure point can thus be felt even better or more strongly by the operator.
  • the receptacle preferably consists of a rubber grommet which ensures particularly simple and reliable frictional adhesion of the liquid receiver and seals the liquid receiver to the piston. If the receptacle consists of two support surfaces arranged at a distance from one another, the liquid receiver is held particularly tightly and firmly by this two-point support.
  • An adapter for accommodating the liquid receiver creates an adaptability to liquid receivers of different external dimensions.
  • a cylinder part which bears on a shoulder of the outer sleeve and has stops for the compression spring and for the stop ring.
  • This cylinder part can be easily inserted into the outer sleeve of the pipette, which makes assembly easier.
  • the pressure pin connected to the piston has a stop for the compression spring and stop surfaces for limiting the piston stroke, this stop and these stop surfaces can be formed in a simple manner by pushing the pressure pin onto the piston, which also facilitates assembly.
  • An end cap carried by the outer sleeve with a stop for limiting the piston stroke also makes assembly easier.
  • the liquid receiver consists of a microcapillary and a pressure compensation opening is provided in the cylinder.
  • the pipette is therefore suitable for use with self-aspirating microcapillaries.
  • the capillary is transparent, the level of the liquid in the capillary can be observed. By dimensioning the capillary, predetermined amounts of the liquid to be sucked up can be measured simply and precisely. Because the outer sleeve or the adapter is transparent in the area of the capillary, the absorbed liquid can be observed and / or measured over a greater or possibly over the entire length of the capillary.
  • the pressure equalization opening is located in the work area at a point where it is closed immediately after the start of the pressure stroke, the pressure stroke can be used over its greatest possible length when using a microcapillary. This is also possible if the cylinder part described above is used.
  • FIGS. 1 to 3 An embodiment of the invention is shown in FIGS. 1 to 3.
  • the outer body of the pipette consists of an outer sleeve 101, an end cap 102 attached to its rear end and an adapter 111 attached to the front end of the outer sleeve 101.
  • a microcapillary 120 is frictionally engaged in a rubber sleeve 110 which is connected to the outer sleeve 101 kept sealed.
  • the microcapillary 120 protrudes through an opening 121 of the adapter 111.
  • the rubber grommet 110 is inserted into the outer sleeve 1 and has an area of larger diameter at its end, which is delimited by two stop surfaces.
  • stop surfaces lies against the end face of the outer sleeve 1
  • the second stop surface lies against a stop surface of the adapter 111 formed by a shoulder.
  • the rubber grommet 110 is held firmly by these two stop surfaces when the adapter 111 is connected to the outer sleeve 101.
  • outer sleeve 101 In the outer sleeve 101 there is a cylinder part 105, which rests with a shoulder 126 in the interior of the outer sleeve 101.
  • the outer sleeve 101 also has a stop 123 on its inner surface.
  • a piston 104 is guided in a longitudinally displaceable manner in the interior of the cylinder part 105.
  • a sealing ring 109 for sealing the piston against the cylinder part 105.
  • the piston 104 has a larger-diameter collar 127, on which a compression spring 108 is supported.
  • the other end of the compression spring 108 is supported on a stop 128 on the inner surface of the cylinder part 105.
  • the compression spring 108 causes the pipette to perform the suction stroke independently.
  • the piston 104 has at its end a smaller-diameter part, on which a pressure pin 103 is pushed up to a stop.
  • the pressure pin 3 passes through a bore in the end cap 102 and has on its outer surface a stop ring 122 with two stop surfaces 124, 125.
  • the stop surface 125 of the pressure pin 103 bears against the stop surface 126 formed by the end face of the end cap 102.
  • the stop 125, 126 is reached independently by the pipette through the action of the compression spring 108.
  • the stop formed by the stop surfaces 125, 126 thus limits the suction stroke of the pipette.
  • the compression spring 108 is also biased in the position shown in FIG. 1.
  • the end face of the pressure pin 103 forms the stop face 128 against which the compression spring 107 rests.
  • the other end of the compression spring 107 rests on the stop ring 106 which is guided in a longitudinally displaceable manner on the piston and which in turn rests on the larger-diameter collar 127 of the piston 104.
  • the compression spring 107 is biased in the embodiment of the invention shown in FIG. 1.
  • vent hole 105a In the cylinder part 105 there is the vent hole 105a, which creates a connection to the outside air.
  • the vent hole 105a is located in the area of the working volume 132 of the cylinder directly next to the sealing ring 109 of the piston 104, which delimits the working volume 132.
  • a smaller-diameter part 130 of the piston 104 extends into the working volume 132 and terminates with the stop surface 131.
  • the diameter of the part 130 of the piston 104 is selected so that it can penetrate the bore 133 passing through the cylinder part 105.
  • the microcapillary 120 rests on the outer end face of the cylinder part 105.
  • the rubber grommet 110 lies in a ring-shaped manner on the outer end face of the cylinder part 105.
  • the capillary 120 When using the pipette, the capillary 120 is pushed manually through the opening of the adapter 111 and further through the rubber grommet 110 up to the stop on the front face of the cylinder part 105. In this two-point support, the capillary 120 is tight and tight.
  • the piston 104 In the unactuated state shown in FIG. 1, the piston 104 is struck all the way back with the stop surface 125 of the stop ring 122 against the stop surface 126 formed by the end face of the end cap 102 by the force of the compression spring 108. The piston 104 clears the vent hole 105a so that the capillary 120 can now be guided to the liquid with the pipette.
  • the capillary 120 is filled by the capillary action. The pressure equalization takes place via the vent hole 105a. If the adapter 111 is transparent, it can be easily observed that the suction process must be ended when the fill level is soft. This can be supported in that the - transparent - capillary carries 120 markings.
  • the sucked-in liquid can be transferred to another vessel in that the piston 104 is pressed down against the force of the compression spring 108 via the pressure pin 103.
  • the sealing ring 109 at the front end of the piston 104 moves over the vent hole 105a and builds up a slight overpressure in the working space 132 between piston 104 or sealing ring 109 and liquid, which leads to the discharge of the liquid.
  • the volume of the working space and the volume of the absorbed liquid must be in a certain relationship to one another so that the liquid column in the capillary 120 is completely “blown out” when the capillary 120 is emptied, without air returning into the capillary 120.
  • the piston 104 At the end of the first stroke, the piston 104 abuts with its stop ring 106 against the stop 134 formed by the end face of the cylinder part 105, as shown in FIG. 2.
  • the front end 131 of the smaller-diameter part 130 of the piston 104 is now almost at the rear end of the capillary 120.
  • the smaller-diameter part 130 of the piston 104 partially penetrates the bore 133 at the end of the cylinder part 105.
  • the relatively high pretensioning force of the compression spring 107 must be overcome like a pressure point. This begins the ejection phase of the capillary 120.
  • the movable stop ring 106 runs up to the block length of the compression spring 108 against the front of the pressure pin 103.
  • the stroke is ended when the stop surface 124 of the stop ring 122 of the pressure pin 103 strikes the stop surface 123 in the interior of the outer sleeve 101, as shown in FIG. 3.
  • the piston 104 sits with the end face 131 of the smaller-diameter part 130 in the exemplary embodiment shown about 2 minutes before the constriction of the rubber nozzle 110, and the capillary 120 falls out of its own accord when the pipette is held vertically.
  • FIGS. 1 to 3 shows a pipette which holds the capillary tightly and axially, so that it fills itself when a liquid surface is touched.

Landscapes

  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)

Claims (14)

1. Pipette comportant
une chemise extérieure (101),
une prise de liquide (120) qui est tenue dans un logement (110) qui la tient par friction et forme un joint étanche,
ainsi qu'un piston (104) qui est relié à un plongeur (103) qui sort de la chemise extérieure (101) qui est guidée de manière à pouvoir se déplacer dans le sens longitudinal dans un cylindre (105, 105a) lequel s'appuie par un premier ressort de pression (108) contre la chemise extérieure et qui peut être projeté vers l'extérieur par l'intermédiaire de l'extrémité de la course de pression pour faire sortir la prise de liquide (120) du logement (110),
la course du piston (104) étant limitée par des butées (122, 124, 125, 123), caractérisée en ce que un deuxième ressort de pression (107) est prévu, qui s'appuie d'une part contre le piston (104) et d'autre part contre un anneau de butée (106) qui s'appuie pendant la course de pression à une butée (127) du piston (104) et ensuite à une butée de la chemise extérieure (134).
2. Pipette selon la revendication 1, caractérisée en ce que le deuxième ressort de pression (107) est pré-contraint et/ou plus dur que le premier ressort de pression (108).
3. Pipette selon les revendications 1 ou 2, caractérisée en ce que le logement consiste en une douille en caoutchouc (110).
4. Pipette selon l'une des revendications 1, 2 ou 3, caractérisée en ce que le logement (110) consiste en deux surfaces d'appui (110, 121) disposées de manière espacée l'une de l'autre.
5. Pipette selon l'une des revendications précédentes, caractérisée en ce qu'une partie de la chemise extérieure (101) de la pipette est formée par un adaptateur (111) pour la prise de liquide (120).
6. Pipette selon l'une des revendications précédentes, caractérisée en ce qu'une partie cylindrique (105) est prévue, qui s'appuie contre un gradin (126) de la chemise extérieure (101) et présente des butées (129, 134) pour le premier ressort de pression (108) et pour l'anneau de butée (106).
7. Pipette selon l'une des revendications précédentes, caractérisée en ce que le plongeur (103) raccordé au piston (104) présente une butée (128) pour le deuxième ressort de pression (107) et des surfaces de butée (124,125) pour la délimitation de la course du piston.
8. Pipette selon l'une des revendications précédentes, caractérisée en ce que la chemise extérieure (101) porte un capuchon terminal (102) avec une butée (126) pour la délimitation de la course du piston.
9. Pipette selon l'une des revendications précédentes, caractérisée en ce qu'une ouverture d'équilibrage de la pression (105a) est prévue dans le cylindre (105).
10. Pipette selon l'une des revendications précédentes, caractérisée en ce que la prise de liquide (120) consiste en une microcapillaire.
11. Pipette selon la revendication 10, caractérisée en ce que la microcapiliaire (120) est transparente. -
12. Pipette selon les revendications 10 ou 11, caractérisée en ce que la microcapillaire (120) est d'une mesure déterminée et/ou est marquée d'une graduation volumétrique.
13. Pipette selon l'une des revendications précédentes, caractérisée en ce que la chemise extérieure (101) et/ou l'adaptateur (111) sont transparents au niveau de la prise de liquide (120).
14. Pipette selon l'une des revendications précédentes, caractérisée en ce que l'ouverture d'équilibrage de la pression (105a) située dans l'espace de travail (132) en un endroit où elle peut être fermée immédiatement après le début de la course de pression et que la partie cylindrique (105) présente l'ouverture d'équilibrage de la pression (105a).
EP86117244A 1985-12-12 1986-12-11 Pipette Expired - Lifetime EP0232517B1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT86117244T ATE50518T1 (de) 1985-12-12 1986-12-11 Pipette.

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE3543950 1985-12-12
DE3543950 1985-12-12
DE19863614085 DE3614085A1 (de) 1985-12-12 1986-04-25 Pipette
DE3614085 1986-04-25

Publications (3)

Publication Number Publication Date
EP0232517A2 EP0232517A2 (fr) 1987-08-19
EP0232517A3 EP0232517A3 (en) 1988-06-22
EP0232517B1 true EP0232517B1 (fr) 1990-02-28

Family

ID=25838773

Family Applications (1)

Application Number Title Priority Date Filing Date
EP86117244A Expired - Lifetime EP0232517B1 (fr) 1985-12-12 1986-12-11 Pipette

Country Status (3)

Country Link
US (1) US4784834A (fr)
EP (1) EP0232517B1 (fr)
DE (2) DE3614085A1 (fr)

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US5104625A (en) * 1989-10-04 1992-04-14 Drummond Scientific Company Pipetter device
DE4341229C2 (de) * 1993-12-03 1995-09-07 Eppendorf Geraetebau Netheler Pipettensystem
US5623106A (en) * 1995-06-06 1997-04-22 Johnson & Johnson Clinical Diagnostics, Inc. Method and apparatus for forming disposable tips in an analyzer
DE19922285A1 (de) * 1999-05-14 2000-11-16 Febit Ferrarius Biotech Gmbh Probengefäß
GB9917325D0 (en) 1999-07-23 1999-09-22 Clinical Diagnostic Chemicals Apparatus for collecting a liquid sample
DE10011235C2 (de) * 2000-03-08 2002-08-08 Max Planck Gesellschaft Ausstechvorrichtung zur Probenaufnahme und Verfahren zur Probenaufnahme
DE102005033378B4 (de) * 2005-07-16 2012-05-31 Eppendorf Ag Kolbenhubpipette
DE102005041183B3 (de) * 2005-08-31 2007-01-04 Eppendorf Ag Pipettiervorrichtung
JP5122091B2 (ja) 2006-06-13 2013-01-16 ユニバーサル・バイオ・リサーチ株式会社 担体封入変形容器、担体封入変形容器処理装置、および担体封入変形容器処理方法
MX2010003578A (es) 2007-10-02 2010-06-09 Theranos Inc Dispositivos modulares de punto de cuidado y usos de los mismos.
GB2467929A (en) * 2009-02-19 2010-08-25 Nordiag Asa Pipette with bellows
CN102740978A (zh) * 2009-12-18 2012-10-17 扎芬纳股份公司 微量移液管
US8372354B2 (en) * 2010-07-19 2013-02-12 Chevron U.S.A. Inc. Multiphase contact and distribution apparatus for hydroprocessing
TWI690594B (zh) 2011-01-21 2020-04-11 美商賽瑞諾斯Ｉｐ有限責任公司 樣本使用最大化之系統及方法
US9619627B2 (en) 2011-09-25 2017-04-11 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US9268915B2 (en) 2011-09-25 2016-02-23 Theranos, Inc. Systems and methods for diagnosis or treatment
US8435738B2 (en) 2011-09-25 2013-05-07 Theranos, Inc. Systems and methods for multi-analysis
US8475739B2 (en) 2011-09-25 2013-07-02 Theranos, Inc. Systems and methods for fluid handling
US8840838B2 (en) 2011-09-25 2014-09-23 Theranos, Inc. Centrifuge configurations
US20140170735A1 (en) 2011-09-25 2014-06-19 Elizabeth A. Holmes Systems and methods for multi-analysis
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
US10012664B2 (en) 2011-09-25 2018-07-03 Theranos Ip Company, Llc Systems and methods for fluid and component handling
US9250229B2 (en) 2011-09-25 2016-02-02 Theranos, Inc. Systems and methods for multi-analysis
AU2013205139B2 (en) * 2011-09-25 2016-03-17 Labrador Diagnostics Llc Systems and methods for multi-analysis
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
CA2938882A1 (fr) * 2014-02-04 2015-08-13 Austen Bioinnovation Institute In Akron Piston pour pipette seringue a faible volume
US9751082B1 (en) 2014-05-22 2017-09-05 Dunsong Yang Multi-actuated micro-pipette controller and associated use thereof
DE102016109536A1 (de) * 2016-05-24 2017-11-30 Kabe-Labortechnik Gmbh Kapillarentleerungshilfe

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Also Published As

Publication number Publication date
DE3669118D1 (en) 1990-04-05
EP0232517A3 (en) 1988-06-22
DE3614085A1 (de) 1987-06-19
US4784834A (en) 1988-11-15
EP0232517A2 (fr) 1987-08-19
DE3614085C2 (fr) 1989-09-07

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