EP0232517B1 - Pipette - Google Patents

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Publication number
EP0232517B1
EP0232517B1 EP86117244A EP86117244A EP0232517B1 EP 0232517 B1 EP0232517 B1 EP 0232517B1 EP 86117244 A EP86117244 A EP 86117244A EP 86117244 A EP86117244 A EP 86117244A EP 0232517 B1 EP0232517 B1 EP 0232517B1
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EP
European Patent Office
Prior art keywords
piston
pipette according
stop
pipette
compression spring
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
EP86117244A
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German (de)
French (fr)
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EP0232517A2 (en
EP0232517A3 (en
Inventor
Adolf M. Hirschmann
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Glasgeratebau Hirschmann
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Glasgeratebau Hirschmann
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Publication date
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Priority to AT86117244T priority Critical patent/ATE50518T1/en
Publication of EP0232517A2 publication Critical patent/EP0232517A2/en
Publication of EP0232517A3 publication Critical patent/EP0232517A3/en
Application granted granted Critical
Publication of EP0232517B1 publication Critical patent/EP0232517B1/en
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    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids

Definitions

  • the invention relates to a pipette with a liquid receiver according to the preamble of claim 1.
  • Such a pipette is known from DE-A-2 159 541.
  • the known pipette has a piston which is guided in a longitudinally displaceable manner in a cylinder, which is connected to a pressure pin protruding from the outer sleeve of the pipette, which is supported on the outer sleeve by a first compression spring and which can be moved beyond the end of the pressure stroke, to move the liquid reamer out of its receptacle. Furthermore, the stroke of the piston is limited by stops.
  • EP-A-0 155 087 shows a pipette with a piston which is guided in a cylinder in a longitudinally displaceable manner and which can be moved beyond the end of the pressure stroke in order to move the liquid receiver out of its receptacle.
  • DE-A-2 851 532 shows a pipette with an elastic bellows, in which the stroke of the bellows is limited by predetermined stops.
  • the bellows is biased in the area in which it has a sufficiently large restoring force between the stops in such a way that it returns to a rest position predetermined by one of the stops without external aids.
  • liquid transducers in particular microcapillaries, are often used for such work, which have to be manually inserted into the pipette.
  • the piston which is longitudinally displaceable in the cylinder, beyond the end of the pressure stroke in order to move the liquid receiver out of its receptacle.
  • the liquid receiver is held in the receptacle by friction, so that it can be moved out of the receptacle by pressure in the longitudinal direction.
  • the liquid force transducer can be removed from the receptacle by hand and separated from the pipette without touching it. Because the liquid receiver is held in a sealing manner in the receptacle, the overpressure required to eject the liquid in the liquid receiver can form during the pressure stroke.
  • the object of the invention is to simplify and improve the handling of the pipette specified at the outset.
  • a second compression spring is provided, which is supported on the one hand on the piston and on the other hand on a stop ring which bears against a stop of the piston during the pressure stroke and subsequently against a stop of the outer sleeve.
  • the force of the second compression spring must be overcome like a pressure point after the end of the pressure stroke when the piston is further depressed, as a result of which the operator can feel the end of the pressure stroke and the start of the ejection phase of the liquid sensor in a reliable manner. This simplifies and improves the handling of the pipette according to the invention.
  • the advantage just described is further increased.
  • the pressure point can thus be felt even better or more strongly by the operator.
  • the receptacle preferably consists of a rubber grommet which ensures particularly simple and reliable frictional adhesion of the liquid receiver and seals the liquid receiver to the piston. If the receptacle consists of two support surfaces arranged at a distance from one another, the liquid receiver is held particularly tightly and firmly by this two-point support.
  • An adapter for accommodating the liquid receiver creates an adaptability to liquid receivers of different external dimensions.
  • a cylinder part which bears on a shoulder of the outer sleeve and has stops for the compression spring and for the stop ring.
  • This cylinder part can be easily inserted into the outer sleeve of the pipette, which makes assembly easier.
  • the pressure pin connected to the piston has a stop for the compression spring and stop surfaces for limiting the piston stroke, this stop and these stop surfaces can be formed in a simple manner by pushing the pressure pin onto the piston, which also facilitates assembly.
  • An end cap carried by the outer sleeve with a stop for limiting the piston stroke also makes assembly easier.
  • the liquid receiver consists of a microcapillary and a pressure compensation opening is provided in the cylinder.
  • the pipette is therefore suitable for use with self-aspirating microcapillaries.
  • the capillary is transparent, the level of the liquid in the capillary can be observed. By dimensioning the capillary, predetermined amounts of the liquid to be sucked up can be measured simply and precisely. Because the outer sleeve or the adapter is transparent in the area of the capillary, the absorbed liquid can be observed and / or measured over a greater or possibly over the entire length of the capillary.
  • the pressure equalization opening is located in the work area at a point where it is closed immediately after the start of the pressure stroke, the pressure stroke can be used over its greatest possible length when using a microcapillary. This is also possible if the cylinder part described above is used.
  • FIGS. 1 to 3 An embodiment of the invention is shown in FIGS. 1 to 3.
  • the outer body of the pipette consists of an outer sleeve 101, an end cap 102 attached to its rear end and an adapter 111 attached to the front end of the outer sleeve 101.
  • a microcapillary 120 is frictionally engaged in a rubber sleeve 110 which is connected to the outer sleeve 101 kept sealed.
  • the microcapillary 120 protrudes through an opening 121 of the adapter 111.
  • the rubber grommet 110 is inserted into the outer sleeve 1 and has an area of larger diameter at its end, which is delimited by two stop surfaces.
  • stop surfaces lies against the end face of the outer sleeve 1
  • the second stop surface lies against a stop surface of the adapter 111 formed by a shoulder.
  • the rubber grommet 110 is held firmly by these two stop surfaces when the adapter 111 is connected to the outer sleeve 101.
  • outer sleeve 101 In the outer sleeve 101 there is a cylinder part 105, which rests with a shoulder 126 in the interior of the outer sleeve 101.
  • the outer sleeve 101 also has a stop 123 on its inner surface.
  • a piston 104 is guided in a longitudinally displaceable manner in the interior of the cylinder part 105.
  • a sealing ring 109 for sealing the piston against the cylinder part 105.
  • the piston 104 has a larger-diameter collar 127, on which a compression spring 108 is supported.
  • the other end of the compression spring 108 is supported on a stop 128 on the inner surface of the cylinder part 105.
  • the compression spring 108 causes the pipette to perform the suction stroke independently.
  • the piston 104 has at its end a smaller-diameter part, on which a pressure pin 103 is pushed up to a stop.
  • the pressure pin 3 passes through a bore in the end cap 102 and has on its outer surface a stop ring 122 with two stop surfaces 124, 125.
  • the stop surface 125 of the pressure pin 103 bears against the stop surface 126 formed by the end face of the end cap 102.
  • the stop 125, 126 is reached independently by the pipette through the action of the compression spring 108.
  • the stop formed by the stop surfaces 125, 126 thus limits the suction stroke of the pipette.
  • the compression spring 108 is also biased in the position shown in FIG. 1.
  • the end face of the pressure pin 103 forms the stop face 128 against which the compression spring 107 rests.
  • the other end of the compression spring 107 rests on the stop ring 106 which is guided in a longitudinally displaceable manner on the piston and which in turn rests on the larger-diameter collar 127 of the piston 104.
  • the compression spring 107 is biased in the embodiment of the invention shown in FIG. 1.
  • vent hole 105a In the cylinder part 105 there is the vent hole 105a, which creates a connection to the outside air.
  • the vent hole 105a is located in the area of the working volume 132 of the cylinder directly next to the sealing ring 109 of the piston 104, which delimits the working volume 132.
  • a smaller-diameter part 130 of the piston 104 extends into the working volume 132 and terminates with the stop surface 131.
  • the diameter of the part 130 of the piston 104 is selected so that it can penetrate the bore 133 passing through the cylinder part 105.
  • the microcapillary 120 rests on the outer end face of the cylinder part 105.
  • the rubber grommet 110 lies in a ring-shaped manner on the outer end face of the cylinder part 105.
  • the capillary 120 When using the pipette, the capillary 120 is pushed manually through the opening of the adapter 111 and further through the rubber grommet 110 up to the stop on the front face of the cylinder part 105. In this two-point support, the capillary 120 is tight and tight.
  • the piston 104 In the unactuated state shown in FIG. 1, the piston 104 is struck all the way back with the stop surface 125 of the stop ring 122 against the stop surface 126 formed by the end face of the end cap 102 by the force of the compression spring 108. The piston 104 clears the vent hole 105a so that the capillary 120 can now be guided to the liquid with the pipette.
  • the capillary 120 is filled by the capillary action. The pressure equalization takes place via the vent hole 105a. If the adapter 111 is transparent, it can be easily observed that the suction process must be ended when the fill level is soft. This can be supported in that the - transparent - capillary carries 120 markings.
  • the sucked-in liquid can be transferred to another vessel in that the piston 104 is pressed down against the force of the compression spring 108 via the pressure pin 103.
  • the sealing ring 109 at the front end of the piston 104 moves over the vent hole 105a and builds up a slight overpressure in the working space 132 between piston 104 or sealing ring 109 and liquid, which leads to the discharge of the liquid.
  • the volume of the working space and the volume of the absorbed liquid must be in a certain relationship to one another so that the liquid column in the capillary 120 is completely “blown out” when the capillary 120 is emptied, without air returning into the capillary 120.
  • the piston 104 At the end of the first stroke, the piston 104 abuts with its stop ring 106 against the stop 134 formed by the end face of the cylinder part 105, as shown in FIG. 2.
  • the front end 131 of the smaller-diameter part 130 of the piston 104 is now almost at the rear end of the capillary 120.
  • the smaller-diameter part 130 of the piston 104 partially penetrates the bore 133 at the end of the cylinder part 105.
  • the relatively high pretensioning force of the compression spring 107 must be overcome like a pressure point. This begins the ejection phase of the capillary 120.
  • the movable stop ring 106 runs up to the block length of the compression spring 108 against the front of the pressure pin 103.
  • the stroke is ended when the stop surface 124 of the stop ring 122 of the pressure pin 103 strikes the stop surface 123 in the interior of the outer sleeve 101, as shown in FIG. 3.
  • the piston 104 sits with the end face 131 of the smaller-diameter part 130 in the exemplary embodiment shown about 2 minutes before the constriction of the rubber nozzle 110, and the capillary 120 falls out of its own accord when the pipette is held vertically.
  • FIGS. 1 to 3 shows a pipette which holds the capillary tightly and axially, so that it fills itself when a liquid surface is touched.

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  • Health & Medical Sciences (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Devices For Use In Laboratory Experiments (AREA)
  • Sampling And Sample Adjustment (AREA)

Description

Die Erfindung betrifft eine Pipette mit einem Flüssigkeitsaufnehmer nach dem Oberbegriff des Anspruchs 1.The invention relates to a pipette with a liquid receiver according to the preamble of claim 1.

Eine derartige Pipette ist aus der DE-A-2 159 541 bekannt. Die vorbekannte Pipette besitzt einen Kolben, der in einem Zylinder längsverschieblich geführt ist, der mit einem aus der Außenhülse der Pipette herausragenden Druckstift verbunden ist, der durch eine erste Druckfeder an der Außenhülse abgestützt ist und der über das Ende des Druckhubes hinaus bewegt werden kann, um den Flüssigkeitsaufriehmer aus seiner Aufnahme herauszubewegen. Weiterhin ist der Hub des Kolbens durch Anschläge begrenzt.Such a pipette is known from DE-A-2 159 541. The known pipette has a piston which is guided in a longitudinally displaceable manner in a cylinder, which is connected to a pressure pin protruding from the outer sleeve of the pipette, which is supported on the outer sleeve by a first compression spring and which can be moved beyond the end of the pressure stroke, to move the liquid reamer out of its receptacle. Furthermore, the stroke of the piston is limited by stops.

Die EP-A-0 155 087 zeigt eine Pipette ,mit einem Kolben, der in einem Zylinder längsverschieblich geführt ist und der über das Ende des Druckhubes hinaus bewegt werden kann, um den Flüssigkeitsaufnehmer aus seiner Aufnahme herauszubewegen.EP-A-0 155 087 shows a pipette with a piston which is guided in a cylinder in a longitudinally displaceable manner and which can be moved beyond the end of the pressure stroke in order to move the liquid receiver out of its receptacle.

Die DE-A-2 851 532 zeigt eine Pipette mit einem elastischen Balg, bei der der Hub des Balges von vorgegebenen Anschlägen begrenzt ist. Der Balg ist in dem Bereich, in dem er eine ausreichend große Rückstellkraft besitzt, zwischen den Anschlägen so vorgespannt, daß er ohne externe Hilfsmittel in eine durch einen der Anschläge vorgegebene Ruhestellung zurückkehrt.DE-A-2 851 532 shows a pipette with an elastic bellows, in which the stroke of the bellows is limited by predetermined stops. The bellows is biased in the area in which it has a sufficiently large restoring force between the stops in such a way that it returns to a rest position predetermined by one of the stops without external aids.

Wenn mit einer Pipette bakterizide Stoffe oder toxische Reagenzien behandelt werden sollen, muß gewährleistet sein, daß die Bedienungsperson nicht mit derartigen Substanzen in Berührung kommt. Vielfach werden jedoch für solche Arbeiten Flüssigkeftsaufnehmer, insbesondere Mikrokapillaren, verwendet, die manuell in die Pipette eingesetzt werden müssen. Bei der eingangs erwähnten Pipette ist es möglich, den in dem Zylinder längsverschieblich geführten Kolben über das Ende des Druckhubes hinaus zu bewegen, um den Flüssigkeitsaufnehmer aus seiner Aufnahme herauszubewegen. Der Flüssigkeitsaufnehmer ist in der Aufnahme reibschlüssig gehalten, so daß er durch einen Druck in Längsrichtung aus der Aufnahme herausbewegt werden kann. Weiterhin kann der Flüssigkeftsaufnehmer ohne Berührung von Hand aus der Aufnahme entfernt und von der Pipette getrennt werden. Dadurch, daß der Flüssigkeitsaufnehmer in der Aufnahme abdichtend gehalten ist, kann sich während des Druckhubes der zum Ausstoß der in dem Flüssigkeitsaufnehmer befindlichen Flüssigkeit erforderliche Überdruck bilden.If bactericidal substances or toxic reagents are to be treated with a pipette, it must be ensured that the operator does not come into contact with such substances. However, liquid transducers, in particular microcapillaries, are often used for such work, which have to be manually inserted into the pipette. In the case of the pipette mentioned at the outset, it is possible to move the piston, which is longitudinally displaceable in the cylinder, beyond the end of the pressure stroke in order to move the liquid receiver out of its receptacle. The liquid receiver is held in the receptacle by friction, so that it can be moved out of the receptacle by pressure in the longitudinal direction. Furthermore, the liquid force transducer can be removed from the receptacle by hand and separated from the pipette without touching it. Because the liquid receiver is held in a sealing manner in the receptacle, the overpressure required to eject the liquid in the liquid receiver can form during the pressure stroke.

Aufgabe der Erfindung ist es, die Handhabung der eingangs angegebenen Pipette zu vereinfachen und zu verbessern.The object of the invention is to simplify and improve the handling of the pipette specified at the outset.

Erfindungsgemäß wird diese Aufgabe durch die im kennzeichnenden Teil des Anspruchs 1 angegebenen Merkmale gelöst. Es ist eine zweite Druckfeder vorgesehen, die sich einerseits an dem Kolben und andererseits an einem Anschlagring abstützt, welcher während des Druckhubes an einem Anschlag des Kolbens und anschliessend an einem Anschlag der Außenhülse anliegt. Dadurch muß nach dem Ende des Druckhubes beim weiteren Niederdrücken des Kolbens druckpunktartig die Kraft der zweiten Druckfeder überwunden werden, wodurch der Bedienungsperson auf zuverlässige Weise fühlbar das Ende des Druckhubes und der Beginn der Ausstoßphase des Flüssigkeitsaufnehmers angezeigt wird. Hierdurch wird die Handhabung der erfindungsgemäßen Pipette erleichtert und verbessert.According to the invention, this object is achieved by the features specified in the characterizing part of claim 1. A second compression spring is provided, which is supported on the one hand on the piston and on the other hand on a stop ring which bears against a stop of the piston during the pressure stroke and subsequently against a stop of the outer sleeve. As a result, the force of the second compression spring must be overcome like a pressure point after the end of the pressure stroke when the piston is further depressed, as a result of which the operator can feel the end of the pressure stroke and the start of the ejection phase of the liquid sensor in a reliable manner. This simplifies and improves the handling of the pipette according to the invention.

Vorteilhafte Weiterbildungen der Erfindung sind in den abhängiger Ansprüchen beschrieben.Advantageous developments of the invention are described in the dependent claims.

Wenn die zweite Druckfeder vorgespannt und/oder härter als die erste Druckfeder ist, wird der soeben beschriebene Vorteil noch verstärkt. Der Druckpunkt ist dadurch für die Bedienungsperson noch besser bzw. stärker fühlbar.If the second compression spring is biased and / or harder than the first compression spring, the advantage just described is further increased. The pressure point can thus be felt even better or more strongly by the operator.

Vorzugsweise besteht die Aufnahme aus einer Gummitülle, die eine besonders einfache und sichere reibschlüssige Hafterung des Flüssigkeitsaufnehmers gewährleistet und den Flüssigkeitsaufnehmer zum Kolben abdichtet. Wenn die Aufnahme aus zwei im Abstand voneinander angeordneten Auflageflächen besteht, wird der Flüssigkeitsaufnehmer durch diese Zweipunktauflage besonders dicht und fest gehalten. Durch einen Adapter für die Aufnahme des Flüssigkeitsaufnehmers wird eine Anpassungsmöglickeit an Flüssigkeitsaufnehmer verschiedener Außenabmessungen geschaffen.The receptacle preferably consists of a rubber grommet which ensures particularly simple and reliable frictional adhesion of the liquid receiver and seals the liquid receiver to the piston. If the receptacle consists of two support surfaces arranged at a distance from one another, the liquid receiver is held particularly tightly and firmly by this two-point support. An adapter for accommodating the liquid receiver creates an adaptability to liquid receivers of different external dimensions.

In vorteilhafter Weiterbildung der Erfindung ist ein Zylinderteil vorgesehen, das an einem Absatz der Außenhülse anliegt und Anschläge für die Druckfeder und für den Anschlagring aufweist. Dieser Zylinderteil kann auf einfache Weise in die Außenhülse der Pipette eingesteckt werden, was die Montage erleichtert.In an advantageous development of the invention, a cylinder part is provided which bears on a shoulder of the outer sleeve and has stops for the compression spring and for the stop ring. This cylinder part can be easily inserted into the outer sleeve of the pipette, which makes assembly easier.

Wenn der mit dem Kolben verbundene Druckstift einen Anschlag für die Druckfeder und Anschlagflächen für die Begrenzung des Kolbenhubes aufweist, können dieser Anschlag und diese Anschlagflächen auf einfache Weise dadurch gebildet werden, daß der Druckstift auf den Kolben aufgesteckt wird, was ebenfalls die Montage erleichtert. Eine von der Außenhülse getragene Endkappe mit einem Anschlag zur Begrenzung des Kolbenhubes führt auch zu einer Montageerleichterung.If the pressure pin connected to the piston has a stop for the compression spring and stop surfaces for limiting the piston stroke, this stop and these stop surfaces can be formed in a simple manner by pushing the pressure pin onto the piston, which also facilitates assembly. An end cap carried by the outer sleeve with a stop for limiting the piston stroke also makes assembly easier.

In vorteilhafter Weiterbildung der Erfindung besteht der Flüssigkeitsaufnehmer aus einer Mikrokapillare und ist eine Druckausgleichsöffnung in dem Zylinder vorgesehen. Die Pipette Ist damit geeignet, mit selbst aufsaugenden Mikrokapillaren verwendet zu werden.In an advantageous development of the invention, the liquid receiver consists of a microcapillary and a pressure compensation opening is provided in the cylinder. The pipette is therefore suitable for use with self-aspirating microcapillaries.

Wenn die Kapillare durchsichtig ist, kann der Füllstand der Flüssigkeit in der Kapillare beobachtet werden. Durch eine Bemaßung der Kapillare können vorbestimmte Mengen der aufzusaugenden Flüssigkeit einfach und genau abgemessen werden. Dadurch, daß die Außenhülse bzw. der Adapter im Bereich der Kapillare durchsichtig ist, kann die aufgesaugte Flüssigkeit über eine größere oder ggf. über die gesamte Länge der Kapillare beobachtet und/oder abgemessen werden.If the capillary is transparent, the level of the liquid in the capillary can be observed. By dimensioning the capillary, predetermined amounts of the liquid to be sucked up can be measured simply and precisely. Because the outer sleeve or the adapter is transparent in the area of the capillary, the absorbed liquid can be observed and / or measured over a greater or possibly over the entire length of the capillary.

Wenn sich die Druckausgleichsöffnung im Arbeitsraum an einer Stelle befindet, an der sie unmittelbar nach Beginn des Druckhubes geschlossen wird, kann bei Verwendung einer Mikrokapillare der Druckhub über seine größtmögliche Länge ausgenutzt werden. Dies ist auch dann möglich, wenn der oben beschriebene Zylinderteil verwendet wird.If the pressure equalization opening is located in the work area at a point where it is closed immediately after the start of the pressure stroke, the pressure stroke can be used over its greatest possible length when using a microcapillary. This is also possible if the cylinder part described above is used.

Ausführungsbeispiele der Erfindung werden nachfolgend anhand der beigefügten Zeichnungen beschrieben. In diesen zeigen

  • Fig. 1 eine Ausführungsform der Erfindung vor Beginn des Druckhubes,
  • Fig. 2 die Ausführungsform nach Fig. 1 am Ende des Druckhubes und
  • Fig. 3 die Ausführungsform der Erfindung nach den Fig. 1 und 2 nach Ausführung der den Flüssigkeitsaufnehmer aus der Aufnahme herausbewegenden Bewegung.
Embodiments of the invention are described below with reference to the accompanying drawings. Show in these
  • 1 shows an embodiment of the invention before the start of the pressure stroke,
  • Fig. 2 shows the embodiment of FIG. 1 at the end of the pressure stroke and
  • Fig. 3 shows the embodiment of the invention according to FIGS. 1 and 2 after execution of the movement moving the liquid receiver out of the receptacle.

In den Fig. 1 bis 3 ist eine Ausführungsform der Erfindung gezeigt. Der Außenkörper der Pipette besteht aus einer Außenhülse 101, einer an deren hinterem Ende befestigten Endkappe 102 und aus einem am vorderen Ende der Außenhülse 101 befestigten Adapter 111. Eine Mikrokapillare 120 ist in einer Gummittülle 110, die mit der Außenhülse 101 verbunden ist, reibschlüssig und abdichtend gehalten. Die Mikrokapillare 120 ragt durch eine Öffnung 121 des Adapters 111. Die Gummitülle 110 wird in die Außenhülse 1 eingeschoben und weist an ihrem Ende einen Bereich größeren Durchmessers auf, der von zwei Anschlagflächen begrenzt ist. Eine dieser Anschlagflächen liegt an der Stirnfläche der Außenhülse 1 an, die zweite Anschlagfläche liegt an einer durch einen Absatz gebildeten Anschlagfläche des Adapters 111 an. Durch diese beiden Anschlagflächen wird die Gummitülle 110 bei mit der Außenhülse 101 verbundenem Adapter 111 fest gehalten.An embodiment of the invention is shown in FIGS. 1 to 3. The outer body of the pipette consists of an outer sleeve 101, an end cap 102 attached to its rear end and an adapter 111 attached to the front end of the outer sleeve 101. A microcapillary 120 is frictionally engaged in a rubber sleeve 110 which is connected to the outer sleeve 101 kept sealed. The microcapillary 120 protrudes through an opening 121 of the adapter 111. The rubber grommet 110 is inserted into the outer sleeve 1 and has an area of larger diameter at its end, which is delimited by two stop surfaces. One of these stop surfaces lies against the end face of the outer sleeve 1, the second stop surface lies against a stop surface of the adapter 111 formed by a shoulder. The rubber grommet 110 is held firmly by these two stop surfaces when the adapter 111 is connected to the outer sleeve 101.

In der Außenhülse 101 befindet sich ein Zylinderteil 105, welches mit einem Absatz 126 im Inneren der Außenhülse 101 an diese anliegt. Die Außenhülse 101 weist weiterhin an ihrer Innenfläche einen Anschlag 123 auf.In the outer sleeve 101 there is a cylinder part 105, which rests with a shoulder 126 in the interior of the outer sleeve 101. The outer sleeve 101 also has a stop 123 on its inner surface.

Im Inneren des Zylinderteils 105 ist ein Kolben 104 längsverschieblich geführt. In einer Nut des Kolbens 104 befindet sich ein Dichtring 109 zur Abdichtung des Kolbens gegenüber dem Zylinderteil 105. Der Kolben 104 weist einen durchmessergrößeren Bund 127 auf, an dem eine Druckfeder 108 abgestützt Ist. Das andere Ende der Druckfeder 108 stützt sich an einem Anschlag 128 an der Innenfläche des Zylinderteils 105 ab. Die Druckfeder 108 bewirkt die selbständige Ausführung des Saughubes der Pipette.A piston 104 is guided in a longitudinally displaceable manner in the interior of the cylinder part 105. In a groove of the piston 104 there is a sealing ring 109 for sealing the piston against the cylinder part 105. The piston 104 has a larger-diameter collar 127, on which a compression spring 108 is supported. The other end of the compression spring 108 is supported on a stop 128 on the inner surface of the cylinder part 105. The compression spring 108 causes the pipette to perform the suction stroke independently.

Der Kolben 104 weist an seinem Ende einen durchmesserkleineren Teil auf, auf den ein Druckstift 103 bis zu einem Anschlag aufgesteckt ist. Der Dru.ckstift 3 durchsetzt eine Bohrung der Endkappe 102 und weist an seiner Außenfläche einen Anschlagring 122 mit zwei Anschlagflächen 124, 125 auf. In der In Fig. 2 gezeigten Stellung liegt die Anschlagfläche 125 des Druckstifts 103 an der durch die Stirnseite der Endkappe 102 gebildeten Anschlagfläche 126 an. Der Anschlag 125, 126 wird von der Pipette selbständig erreicht durch die Wirkung der Druckfeder 108. Der durch die Anschlagflächen 125, 126 gebildete Anschlag begrenzt also den Saughub der Pipette. Bei dem in Fig. 1 gezeigten Ausführungsbeispiel der Erfindung ist die Druckfeder 108 auch in der in Fig. 1 gezeigten Stellung noch vorgespannt.The piston 104 has at its end a smaller-diameter part, on which a pressure pin 103 is pushed up to a stop. The pressure pin 3 passes through a bore in the end cap 102 and has on its outer surface a stop ring 122 with two stop surfaces 124, 125. In the position shown in FIG. 2, the stop surface 125 of the pressure pin 103 bears against the stop surface 126 formed by the end face of the end cap 102. The stop 125, 126 is reached independently by the pipette through the action of the compression spring 108. The stop formed by the stop surfaces 125, 126 thus limits the suction stroke of the pipette. In the embodiment of the invention shown in FIG. 1, the compression spring 108 is also biased in the position shown in FIG. 1.

Die Stirnfläche des Druckstifts 103 bildet die Anschlagfläche 128, an der die Druckfeder 107 anliegt. Das andere Ende der Druckfeder 107 liegt an dem auf dem Kolben längsverschieblich geführten Anschlagring 106 an, der seinerseits an dem durchmessergrößeren Bund 127 des Kolbens 104 anliegt. Die Druckfeder 107 ist bei dem in Fig. 1 gezeigten Ausführungsbeispiel der Erfindung vorgespannt.The end face of the pressure pin 103 forms the stop face 128 against which the compression spring 107 rests. The other end of the compression spring 107 rests on the stop ring 106 which is guided in a longitudinally displaceable manner on the piston and which in turn rests on the larger-diameter collar 127 of the piston 104. The compression spring 107 is biased in the embodiment of the invention shown in FIG. 1.

In dem Zylinderteil 105 befindet sich die Entlüftungsbohrung 105a, die ein Verbindung zur Au-8enluft herstellt. Die Entlüftungsbohrung 105a befindet sich im Bereich des Arbeitsvolumens 132 des Zylinders unmittelbar neben dem Dichtring 109 des Kolbens 104, der das Arbeitsvolumen 132 begrenzt. In das Arbeitsvolumen 132 erstreckt sich ein durchmesserkleinerer Teil 130 des Kolbens 104, der mit der Anschlagfläche 131 abschließt. Der Durchmesser des Teils 130 des Kolbens 104 ist dabei so gewählt, daß er die den Zylinderteil 105 durchsetzende Bohrung 133 durchdringen kann. An der äußeren Stirnfläche des Zylinderteils 105 liegt die Mikrokapillare 120 an. Weiterhin liegt an der äußeren Stirnfläche des Zylinderteils 105 die Gummitülle 110 ringförmig abschließend an.In the cylinder part 105 there is the vent hole 105a, which creates a connection to the outside air. The vent hole 105a is located in the area of the working volume 132 of the cylinder directly next to the sealing ring 109 of the piston 104, which delimits the working volume 132. A smaller-diameter part 130 of the piston 104 extends into the working volume 132 and terminates with the stop surface 131. The diameter of the part 130 of the piston 104 is selected so that it can penetrate the bore 133 passing through the cylinder part 105. The microcapillary 120 rests on the outer end face of the cylinder part 105. Furthermore, the rubber grommet 110 lies in a ring-shaped manner on the outer end face of the cylinder part 105.

Beim Gebrauch der Pipette wird die Kapillare 120 manuell durch die Öffnung des Adapters 111 und weiterführend durch die Gummitülle 110 bis zum Anschlag an die vordere Stirnseite des Zylinderteils 105 geschoben. In dieser Zweipunktauflage sitzt die Kapillare 120 dicht und fest. In dem in Fig. 1 gezeigten unbetätigten Zustand ist der Kolben 104 ganz nach hinten mit der Anschlagfläche 125 des Anschlagrings 122 an die von der Stirnfläche der Endkappe 102 gebildete Anschlagfläche 126 durch die Kraft der Druckfeder 108 angeschlagen. Der Kolben 104 gibt dabei die Entlüftungsbohrung 105a frei so daß nun die Kapillare 120 mit der Pipette an die Flüssigkeit geführt werden kann. Durch die Kapillarwirkung wird die Kapillare 120 gefülft. Der Druckausgleich erfolgt über die Entlüftungsbohrung 105a. Wenn der Adapter 111 transparent ist, kann gut beobachtet werden, bei weichem Füllstand der Ansaugvorgang beendet werden muß. Dies kann dadurch unterstützt werden, daß die - durchsichtige - Kapillare 120 Markierungen trägt.When using the pipette, the capillary 120 is pushed manually through the opening of the adapter 111 and further through the rubber grommet 110 up to the stop on the front face of the cylinder part 105. In this two-point support, the capillary 120 is tight and tight. In the unactuated state shown in FIG. 1, the piston 104 is struck all the way back with the stop surface 125 of the stop ring 122 against the stop surface 126 formed by the end face of the end cap 102 by the force of the compression spring 108. The piston 104 clears the vent hole 105a so that the capillary 120 can now be guided to the liquid with the pipette. The capillary 120 is filled by the capillary action. The pressure equalization takes place via the vent hole 105a. If the adapter 111 is transparent, it can be easily observed that the suction process must be ended when the fill level is soft. This can be supported in that the - transparent - capillary carries 120 markings.

Nach Beendigung des Ansaugvorgangs kann die angesaugt« Flüssigkeit in ein anderes Gefäß übertragen werden, indem der Kolben 104 über den Druckstift 103 gegen die Kraft der Druckfeder 108 niedergedrückt wird. Dabei bewegt sich der Dichtring 109 am vorderen Ende des Kolbens 104 über die Entlüftungsbohrung 105a hinweg und baut auf seinem weiteren Weg in dem Arbeitsraum 132 zwischen Kolben 104 bzw. Dichtring 109 und Flüssigkeit einen leichten Überdruck auf, der zum Ausstoß der Flüssigkeit führt. Das Volumen des Arbeitsraumes und das Volumen der aufgesaugten Flüssigkeit müssen in einem bestimmten Verhältnis zueinander stehen, damit die in der Kapillare 120 befindliche Flüssigkeitssäule beim Entleeren der Kapillare 120 ohne Rücksprung von Luft in die Kapillare 120 vollständig "ausgeblasen" wird.After the suction process has ended, the sucked-in liquid can be transferred to another vessel in that the piston 104 is pressed down against the force of the compression spring 108 via the pressure pin 103. The sealing ring 109 at the front end of the piston 104 moves over the vent hole 105a and builds up a slight overpressure in the working space 132 between piston 104 or sealing ring 109 and liquid, which leads to the discharge of the liquid. The volume of the working space and the volume of the absorbed liquid must be in a certain relationship to one another so that the liquid column in the capillary 120 is completely “blown out” when the capillary 120 is emptied, without air returning into the capillary 120.

Am Ende des ersten Hubweges liegt der Kolben 104 mit seinem Anschlagring 106 an dem von der Stirnfläche des Zylinderteils 105 gebildeten Anschlag 134 an, wie es in Fig. 2 gezeigt ist. Das vordere Ende 131 des durchmesserkleineren Teils 130 des Kolbens 104 steht nun fast am hinteren Ende der Kapillare 120 an. Der durchmesserkleinere Teil 130 des Kolbens 104 durchsetzt dabei teilweise die Bohrung 133 am Ende des Zylinderteils 105.At the end of the first stroke, the piston 104 abuts with its stop ring 106 against the stop 134 formed by the end face of the cylinder part 105, as shown in FIG. 2. The front end 131 of the smaller-diameter part 130 of the piston 104 is now almost at the rear end of the capillary 120. The smaller-diameter part 130 of the piston 104 partially penetrates the bore 133 at the end of the cylinder part 105.

Beim weiteren Niederdrücken des Kolbens 104 muß druckpunktartig die relativ hohe Vorspannkraft der Druckfeder 107 überwunden werden. Damit beginn die Ausstoßphase der Kapillare 120. Der bewegliche Anschlagring 106 läuft bis fast zur Blocklänge der Druckfeder 108 gegen die Vorderseite des Druckstiftes 103.When the piston 104 is depressed further, the relatively high pretensioning force of the compression spring 107 must be overcome like a pressure point. This begins the ejection phase of the capillary 120. The movable stop ring 106 runs up to the block length of the compression spring 108 against the front of the pressure pin 103.

Der Hub ist beendet, wenn die Anschlagfläche 124 des Anschlagrings 122 des Druckstifts 103 an die Anschlagfläche 123 im Inneren der Außenhülse 101 anschlägt, wie es in Fig. 3 gezeigt ist. In diesem Zustand sitzt der Kolben 104 mit der Stirnfläche 131 des durchmesserkleineren Teils 130 bei dem gezeigten Ausführungsbeispiel etwa 2 min vor der Engstelle der Gummitülle 110, und die Kapillare 120 fällt bei Vertikalhaltung der Pipette von selbst heraus.The stroke is ended when the stop surface 124 of the stop ring 122 of the pressure pin 103 strikes the stop surface 123 in the interior of the outer sleeve 101, as shown in FIG. 3. In this state, the piston 104 sits with the end face 131 of the smaller-diameter part 130 in the exemplary embodiment shown about 2 minutes before the constriction of the rubber nozzle 110, and the capillary 120 falls out of its own accord when the pipette is held vertically.

Durch das in den Fig. 1 bis 3 dargestellte Ausführungsbeispiel der Erfindung wird eine Pipette gezeigt, die die Kapillare dicht und axial festhält, so daß sie sich beim Berühren einer Flüssigkeitsoberfläche von selbst füllt.The exemplary embodiment of the invention shown in FIGS. 1 to 3 shows a pipette which holds the capillary tightly and axially, so that it fills itself when a liquid surface is touched.

Beim Niederdrücken des Druckstifts wird zunächst die eingesaugte Flüssigkeit gleichmäßig langsam und ohne Abreißen des Flüssigkeitsfadens abgegeben. Anschließend wird die Kapillare durch weiteres Niederdrücken des Druckstifts ohne Zuhilfenahme der Hand aus der Pipette ausgestossen.When the pressure pin is pressed down, the liquid that is sucked in is first released evenly slowly and without tearing off the liquid thread. The capillary is then ejected from the pipette by pressing the pressure pin down further without the aid of the hand.

Claims (14)

1. Pipette with
an external sleeve (101),
a liquid take-up (120) which is held in a mount (110) which grips it by frictional force and forms a seal,
and a piston (104) which is connected to a plunger (103) projecting out of the external sleeve (101) and mounted with longitudinal displacement in a cylinder (105, 105a) which presses against the external sleeve (101) by the action of a first compression spring (108) and which can be moved beyond the end of the compression stroke in order to extend the liquid take-up (120) beyond its mount (110),
where the stroke of the piston (104) is limited by stops (122, 124, 125, 123), characterized therein that a second compression spring (107) is provided which rests at one end against the piston (104) and at the other against a stop ring (106) which lies against a stop (127) of the piston (104) during the compression stroke and then against a stop on the external sleeve (134).
2. Pipette according to claim 1, characterized therein that the second compression spring (107) is pretensioned and/or harder than the first compression spring (108).
3. Pipette according to claim 1 or 2, characterized therein that the mount consists of a sealing rubber bushing (110).
4. Pipette according to claim 1, 2 or 3, characterized therein that the mount (110) consists of two supporting surfaces (110, 121) located at a given spacing.
5. Pipette according to any one of the preceding claims, characterized therein that part of the ex- temal sleeve (101) of the pipette is formed into an extension piece (111) for the liquid take-up (120).
6. Pipette according to any one of the preceding claims, characterized therein that a cylinder section (105) is provided which lies against a shoulder (126) of the external sleeve (101) and has stops (129, 134) for the first compression spring (108) and for the stop ring (106).
7. Pipette according to any one of the preceding claims, characterized therein that the plunger (103) connected to the piston (104) has a stop (128) for the second compression spring (107) and stop surfaces (124, 125) to limit the piston stroke.
8. Pipette according to any one of the preceding claims, characterized therein that the external sleeve (101) has a terminal cap (102) with a stop (126) to limit the piston stroke.
9. Pipette according to any one of the preceding claims, characterized therein that a pressure equalizing aperture (105a) is provided in the cylinder (105).
10. Pipette according to any one of the preceding claims, characterized therein that the liquid take-up (120) consists of a microcapillary.
11. Pipette according to claim 10, characterized therein that the microcapillary (120) is transparent.
12. Pipette according to claim 10 or 11, characterized therein that the microcapillary (120) is graduated and/or marked with volumetric marks.
13. Pipette according to any one of the preceding claims, characterized therein that the external sleeve (101) and/or the extension piece (111) is transparent in the region of the liquid take-up (120).
14. Pipette according to any one of the preceding claims, characterized therein that the pressure equalizing aperture (105a) in the working space (132) is at a location where it is closed immediately after the start of the compression stroke and that the pressure equalizing aperture (105a) is on the cylinder section (105).
EP86117244A 1985-12-12 1986-12-11 Pipette Expired - Lifetime EP0232517B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
AT86117244T ATE50518T1 (en) 1985-12-12 1986-12-11 PIPETTE.

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DE3543950 1985-12-12
DE3543950 1985-12-12
DE19863614085 DE3614085A1 (en) 1985-12-12 1986-04-25 PIPETTE
DE3614085 1986-04-25

Publications (3)

Publication Number Publication Date
EP0232517A2 EP0232517A2 (en) 1987-08-19
EP0232517A3 EP0232517A3 (en) 1988-06-22
EP0232517B1 true EP0232517B1 (en) 1990-02-28

Family

ID=25838773

Family Applications (1)

Application Number Title Priority Date Filing Date
EP86117244A Expired - Lifetime EP0232517B1 (en) 1985-12-12 1986-12-11 Pipette

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Country Link
US (1) US4784834A (en)
EP (1) EP0232517B1 (en)
DE (2) DE3614085A1 (en)

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Also Published As

Publication number Publication date
US4784834A (en) 1988-11-15
DE3669118D1 (en) 1990-04-05
EP0232517A2 (en) 1987-08-19
DE3614085A1 (en) 1987-06-19
EP0232517A3 (en) 1988-06-22
DE3614085C2 (en) 1989-09-07

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