EP0144368A1 - Verfahren zur abtrennung von allergenen aus arnikablüten mittels co 2?-hochdruckextraktion - Google Patents

Verfahren zur abtrennung von allergenen aus arnikablüten mittels co 2?-hochdruckextraktion

Info

Publication number
EP0144368A1
EP0144368A1 EP84901998A EP84901998A EP0144368A1 EP 0144368 A1 EP0144368 A1 EP 0144368A1 EP 84901998 A EP84901998 A EP 84901998A EP 84901998 A EP84901998 A EP 84901998A EP 0144368 A1 EP0144368 A1 EP 0144368A1
Authority
EP
European Patent Office
Prior art keywords
arnica
extraction
extracted
flowers
high pressure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP84901998A
Other languages
German (de)
English (en)
French (fr)
Inventor
Hans Pfeiffer
Gerd GÖBEL
Wilfried Welge
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Air Products and Chemicals Inc
Original Assignee
Henkel AG and Co KGaA
Air Products and Chemicals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Henkel AG and Co KGaA, Air Products and Chemicals Inc filed Critical Henkel AG and Co KGaA
Publication of EP0144368A1 publication Critical patent/EP0144368A1/de
Withdrawn legal-status Critical Current

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D11/00—Solvent extraction
    • B01D11/02—Solvent extraction of solids
    • B01D11/0203—Solvent extraction of solids with a supercritical fluid
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • A61K36/28—Asteraceae or Compositae (Aster or Sunflower family), e.g. chamomile, feverfew, yarrow or echinacea
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K39/35—Allergens
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]

Definitions

  • Arnica montana L. (Compositae family) has been one of the most important medicinal plants of the Central European medicinal treasure for many centuries, monographs of arnica flowers (Flores Arnicae) can be found in all German pharmacopoeias. Numerous medicinal preparations contain extracts from Arnica montana. The main focus of the use of arnica preparations is external use. The effect is described as antiseptic and anti-inflammatory and promotes blood coagulation, arnica extracts are therefore mainly used in wound and healing ointments, but also in hair oils, hair lotions, herbal shampoos and the like. When used internally, a breathing and circulatory and absorption-promoting effect is seen.
  • sesquiterpene lactones can be extracted quantitatively by suitable treatment of arnica flowers with liquid or fluid CO 2 , without other ingredients which are important for the pharmacological effect, such as the flavonoid glycosides and part of the essential oil, also being included.
  • the invention thus relates to a process for the separation of allergens from arnica flowers, characterized in that dried arnica flowers are subjected to a high-pressure extraction with supercritical CO 2 at 7o-5oo bar and 31-9o ° C and the extracted allergenic substances and any co-extracted essential oil Deposits pressure and / or temperature reduction.
  • the dried arnica flowers can be broken up by crushing, for example using a smooth roller under high pressure.
  • this has the disadvantage that, in addition to the allergenic sesquiterpene lactones, the essential oil is also extracted quantitatively. It is therefore more advantageous to subject the uncrushed flowers to the extraction, the sesquiterpene lactone component also being removed quantitatively, while a substantial proportion of the essential oil remains in the extracted drug in addition to the other pharmacologically active substances. This is then used in the usual way for the extraction and production of allergen-free arnica extracts, tinctures, etc.
  • the extraction takes place with supercritical CO 2 , at pressures between 7o and 5oo bar, preferably at 3oo bar and temperatures between 31 and 9o ° C, preferably at 5o - 7o ° C.
  • the separation of the extracts takes place by lowering the pressure and temperature. It takes about 3 to 6 hours to extract the uncrushed drug.
  • the determination of the ingredients in the drug before or after the extraction is carried out qualitatively by thin layer chromatography.
  • the completeness of the extraction can be assessed by appropriate examination of the CO 2 extract.
  • Dried arnica flowers (Arnica. Montana, Flos) are extracted for testing for flavonoids with methanol (lg drug with 20 ml of methanol). It is then filtered and ⁇ s filtrate concentrated to 2 ml.
  • the extracts are dissolved in chloroform / methanol (3: 1), 1oo ⁇ l sample are applied to a silica gel GF 254 plate and developed by means of thin layer chromatography with ethyl acetate / formic acid (anhydrous) / acetic acid / H 2 O (100: 11: 11: 25) . Detection is carried out by spraying with a 1% solution of diphenylboryloxyethylamine in methanol.
  • the samples (2o - 3O ⁇ l) are applied to a silica gel F 254 thin-layer plate (Merck) and developed with n-pentane / ether (8:17).
  • the detection takes place in UV light after spraying with
  • the essential oil content is determined in accordance with DAB 8.
  • Sesquiterpene lactones can be detected by thin layer chromatography
  • the extract contained the total amount of sesquiterpene lactones and 2.2 ml / 100 g of essential oil corresponding to 0.14 ml / 100 g of dried raw material. Flavonoid glycosides could not be detected.
  • Sesquiterpene lactones were no longer detectable in the extracted drug, while the essential oil content was 0.02 ml / 100 g. The flavonoid glycosides were completely preserved.

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Microbiology (AREA)
  • Mycology (AREA)
  • Epidemiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Rheumatology (AREA)
  • Pain & Pain Management (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Alternative & Traditional Medicine (AREA)
  • Biotechnology (AREA)
  • Botany (AREA)
  • Medical Informatics (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
EP84901998A 1983-05-27 1984-05-19 Verfahren zur abtrennung von allergenen aus arnikablüten mittels co 2?-hochdruckextraktion Withdrawn EP0144368A1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19833319184 DE3319184A1 (de) 1983-05-27 1983-05-27 Verfahren zur abtrennung von allergenen aus arnikablueten mittels co(pfeil abwaerts)2(pfeil abwaerts)-hochdruckextraktion
DE3319184 1983-05-27

Publications (1)

Publication Number Publication Date
EP0144368A1 true EP0144368A1 (de) 1985-06-19

Family

ID=6199971

Family Applications (1)

Application Number Title Priority Date Filing Date
EP84901998A Withdrawn EP0144368A1 (de) 1983-05-27 1984-05-19 Verfahren zur abtrennung von allergenen aus arnikablüten mittels co 2?-hochdruckextraktion

Country Status (6)

Country Link
EP (1) EP0144368A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
JP (1) JPS60501456A (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
DE (1) DE3319184A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
ES (1) ES8502864A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
IT (1) IT1180170B (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
WO (1) WO1984004683A1 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0464298A1 (en) * 1990-07-05 1992-01-08 INDENA S.p.A. Echinacea extracts, a process for the preparation thereof and formulations containing them

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT1190129B (it) * 1986-06-17 1988-02-10 Indena Spa Procedimento di estrazione di frutti oleaginosi
DE3637826C1 (en) * 1986-11-06 1988-05-11 Hopfenextraktion Hvg Barth Process for extracting natural dyes from walnut shells and henna
AU607027B2 (en) * 1987-12-16 1991-02-21 Unilever Plc Process for the extraction of lactones from lipid material and use of the extract thus obtained for flavouring foodstuffs
NL8900652A (nl) * 1989-03-16 1990-10-16 Leti Lab Primair-toxische stoffen of allergenen, isoleerbaar uit plantaardig materiaal, alsmede werkwijzen ter bereiding ervan en de toepassing ervan.
US5120558A (en) * 1991-05-01 1992-06-09 Norac Technologies Inc. Process for the supercritical extraction and fractionation of spices
CA2072400C (en) 1991-07-05 2003-08-19 Jayaprakash B. Nair Supercritical extraction of taxanes
FR2843125B1 (fr) 2002-08-02 2012-11-16 Coletica Principes actifs stimulant les beta-defensives humaines de type 2 et/ou de type 3, et compositions cosmetiques ou pharmaceutiques comprenant de tels principes actifs

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE789002A (fr) * 1971-09-22 1973-01-15 Sori Soc Rech Ind Procede d'obtention d'un extrait d'arnica montana
DE2844781A1 (de) * 1978-10-13 1980-04-24 Hag Ag Verfahren zur extraktiven bearbeitung von pflanzlichen und tierischen materialien
DE2931395A1 (de) * 1979-08-02 1981-02-26 Henkel Kgaa Verfahren zur herstellung von gewuerzextrakten
DE3105557A1 (de) * 1981-02-16 1982-09-09 Henkel Kgaa "verfahren zur gewinnung von inhaltsstoffen der kamille durch extraktion mit kohlendioxid"

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See references of WO8404683A1 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0464298A1 (en) * 1990-07-05 1992-01-08 INDENA S.p.A. Echinacea extracts, a process for the preparation thereof and formulations containing them

Also Published As

Publication number Publication date
IT1180170B (it) 1987-09-23
ES532847A0 (es) 1985-02-01
JPH0460451B2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html) 1992-09-28
IT8421097A1 (it) 1985-11-25
IT8421097A0 (it) 1984-05-25
ES8502864A1 (es) 1985-02-01
DE3319184A1 (de) 1984-11-29
JPS60501456A (ja) 1985-09-05
WO1984004683A1 (en) 1984-12-06

Similar Documents

Publication Publication Date Title
DE4202657C2 (de) Pharmazeutisch wirksame Zusammensetzung aus Tanacetum parthenium sowie Verfahren zu deren Extraktion und mit der pharmazeutisch wirksamen Zusammensetzung hergestelltes Arzneimittel
DE69713116T2 (de) Pharmazeutische und kosmetische antiaknezusammensetzungen, die pflanzenextrakte (krameria triandra oder mesua ferrea) enthalten
EP0144368A1 (de) Verfahren zur abtrennung von allergenen aus arnikablüten mittels co 2?-hochdruckextraktion
DE3783809T2 (de) Extraktionsverfahren fuer oelhaltige fruechte.
DE3935772C2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
EP0285752B1 (de) Kamillenöle mit hohem Gehalt an natürlichen Polyinen und Verfahren zu deren Herstellung
EP0904092A1 (de) Gereinigter extrakt von harpagophytum procumbens und/oder harpagophytum zeyheri dence, verfahren zu seiner herstellung und seine verwendung
DE3874468T2 (de) Verfahren zur herstellung eines jasminextraktes und der so gewonnene extrakt.
DE2652380A1 (de) Verfahren zur isolation von uteroevakuierenden substanzen aus der zoapatlepflanze
DE69720234T2 (de) Verfahren zur herstellung von einem valeriana officinalis extrakt mit hohem gehalt an valerensäure
DE69710488T2 (de) Verfahren zur herstellung eines ganodericsäure enthaltenden extraktes aus ganoderma lucidum
DE4205783C1 (en) Prepn. of liquid plant extracts, e.g. contg. pharmaceuticals or perfumes - involves use of an aq. soln. of a nonionic surfactant e.g. fatty acid glycerine polyethylene glycol ester
DE3112737C2 (GUID-C5D7CC26-194C-43D0-91A1-9AE8C70A9BFF.html)
DE2165633C3 (de) Geruchsverbesserte isopropanolhaltige Kamillenextrakte
DE4101841C2 (de) Verwendung von Sabal-Extrakten zum Behandeln von Mammakarzinomen
DE2515670A1 (de) Verfahren zur extraktion, isolation und reinigung von praeparaten mit uteroevakuierender wirkung aus der zoapatl- pflanze
DE4221537A1 (de) Hamamelis-Trockenextrakt, Verfahren zu seiner Herstellung und seine Verwendung als Arzneimittel
EP1492550B1 (de) Weidenextrakt
DE10056902A1 (de) Verfahren zur Gewinnung von Triterpenen aus Pflanzen sowie deren Verwendung
DE69307656T2 (de) Aetherisches oel
DE3901275A1 (de) Verfahren zur herstellung von oeligem kamillenextrakt
DE950165C (de) Verfahren zum Gewinnen von Bixinfarbstoff aus Anattosaat (Orleansaat), insbesondere zur Butter- oder Margarinefaerbung
DE69634658T2 (de) Verfahren zur Isolierung eines Wirkstoffes aus Azadirachta Indica zur Verwendung bei der Therapie von Hyperacidität und Magengeschwüren
DE1467977A1 (de) Verfahren zur Gewinnung von Pflanzenextrakten mit pharmakologischer Wirkung
DE2646321A1 (de) Kosmetisches mittel

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

AK Designated contracting states

Designated state(s): AT BE CH DE FR GB LI NL

17P Request for examination filed

Effective date: 19850517

RAP1 Party data changed (applicant data changed or rights of an application transferred)

Owner name: AIR PRODUCTS AND CHEMICALS, INC.

17Q First examination report despatched

Effective date: 19880308

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 19880719

RIN1 Information on inventor provided before grant (corrected)

Inventor name: PFEIFFER, HANS

Inventor name: WELGE, WILFRIED

Inventor name: GOEBEL, GERD