EA201990815A1 - Способ редактирования основания у растений - Google Patents

Способ редактирования основания у растений

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Publication number
EA201990815A1
EA201990815A1 EA201990815A EA201990815A EA201990815A1 EA 201990815 A1 EA201990815 A1 EA 201990815A1 EA 201990815 A EA201990815 A EA 201990815A EA 201990815 A EA201990815 A EA 201990815A EA 201990815 A1 EA201990815 A1 EA 201990815A1
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EA
Eurasian Patent Office
Prior art keywords
base
editing
plants
relates
present
Prior art date
Application number
EA201990815A
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English (en)
Inventor
Саиксиа Гао
Йуан Зонг
Original Assignee
Институте Оф Генетисс Анд Девелопментал Биологй. Чинесе Асадемй Оф Ссиенсес
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Application filed by Институте Оф Генетисс Анд Девелопментал Биологй. Чинесе Асадемй Оф Ссиенсес filed Critical Институте Оф Генетисс Анд Девелопментал Биологй. Чинесе Асадемй Оф Ссиенсес
Publication of EA201990815A1 publication Critical patent/EA201990815A1/ru

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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
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    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
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    • C12N15/09—Recombinant DNA-technology
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
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    • C12N9/14—Hydrolases (3)
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    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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Abstract

Настоящее изобретение относится к области генной инженерии растений. В частности, настоящее изобретение относится к способу редактирования основания у растений. Более конкретно, настоящее изобретение относится к способу выполнения эффективного редактирования основания у целевой последовательности в геноме растения (таком, как сельскохозяйственная культура) посредством белка слияния Cas9-цитидиндезаминазы, а также у растений, продуцированных посредством упомянутого способа, и у их потомства.
EA201990815A 2016-11-14 2017-11-14 Способ редактирования основания у растений EA201990815A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN201610998842 2016-11-14
PCT/CN2017/110841 WO2018086623A1 (en) 2016-11-14 2017-11-14 A method for base editing in plants

Publications (1)

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EA201990815A1 true EA201990815A1 (ru) 2019-09-30

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EA201990815A EA201990815A1 (ru) 2016-11-14 2017-11-14 Способ редактирования основания у растений

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US (1) US11447785B2 (ru)
EP (1) EP3538661A4 (ru)
JP (1) JP2019523011A (ru)
KR (1) KR20190039430A (ru)
CN (1) CN108070611B (ru)
AR (1) AR110075A1 (ru)
AU (1) AU2017358264A1 (ru)
BR (1) BR112019002455A2 (ru)
CA (1) CA3043774A1 (ru)
EA (1) EA201990815A1 (ru)
WO (1) WO2018086623A1 (ru)

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JP2020534795A (ja) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ ファージによって支援される連続的進化（ｐａｃｅ）を用いて塩基編集因子を進化させるための方法および組成物
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US11795443B2 (en) 2017-10-16 2023-10-24 The Broad Institute, Inc. Uses of adenosine base editors
CN110157727A (zh) * 2017-12-21 2019-08-23 中国科学院遗传与发育生物学研究所 植物碱基编辑方法
US20210198330A1 (en) * 2018-05-23 2021-07-01 The Broad Institute, Inc. Base editors and uses thereof
CN108753778B (zh) * 2018-06-01 2021-11-02 上海科技大学 利用碱基编辑修复fbn1t7498c突变的试剂和方法
WO2020020193A1 (zh) * 2018-07-24 2020-01-30 中国科学院遗传与发育生物学研究所 基于人apobec3a脱氨酶的碱基编辑器及其用途
WO2020033083A1 (en) * 2018-08-10 2020-02-13 Cornell University Optimized base editors enable efficient editing in cells, organoids and mice
CN110835629B (zh) * 2018-08-15 2022-07-26 华东师范大学 一种新型碱基转换编辑系统的构建方法及其应用
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CN109652440A (zh) * 2018-12-28 2019-04-19 北京市农林科学院 VQRn-Cas9&PmCDA1&UGI碱基编辑系统在植物基因编辑中的应用
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