EA201170104A1 - Улучшенное получение и хранение белков в растениях - Google Patents

Улучшенное получение и хранение белков в растениях

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Publication number
EA201170104A1
EA201170104A1 EA201170104A EA201170104A EA201170104A1 EA 201170104 A1 EA201170104 A1 EA 201170104A1 EA 201170104 A EA201170104 A EA 201170104A EA 201170104 A EA201170104 A EA 201170104A EA 201170104 A1 EA201170104 A1 EA 201170104A1
Authority
EA
Eurasian Patent Office
Prior art keywords
seed
proteins
plants
storage
improved production
Prior art date
Application number
EA201170104A
Other languages
English (en)
Inventor
Элиот М. Херман
Моника А. Шмидт
Original Assignee
Доналд Дэнфорт Плэнт Сайенс Сентер
Дзе Юнайтед Стейтс Оф Америка, Эс Репрезентед Бай Дзе Секретэри Оф Эгрикалчер
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Доналд Дэнфорт Плэнт Сайенс Сентер, Дзе Юнайтед Стейтс Оф Америка, Эс Репрезентед Бай Дзе Секретэри Оф Эгрикалчер filed Critical Доналд Дэнфорт Плэнт Сайенс Сентер
Publication of EA201170104A1 publication Critical patent/EA201170104A1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Nutrition Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Трансгенное двудольное растение, имеющее недостаточность одного или нескольких запасных белков семян растения, также содержащее трансгенную полинуклеотидную конструкцию, содержащую открытую рамку считывания, функционально связанную с промотором запасного белка и сигнальной последовательностью ER. Полинуклеотидная конструкция кодирует белковый продукт, который может накапливаться в семени на высоком уровне. Также предоставлены способы получения гетерологичного белка в семени растения.
EA201170104A 2008-06-28 2009-06-29 Улучшенное получение и хранение белков в растениях EA201170104A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7661608P 2008-06-28 2008-06-28
PCT/US2009/049097 WO2009158716A1 (en) 2008-06-28 2009-06-29 Improved protein production and storage in plants

Publications (1)

Publication Number Publication Date
EA201170104A1 true EA201170104A1 (ru) 2011-08-30

Family

ID=41444990

Family Applications (1)

Application Number Title Priority Date Filing Date
EA201170104A EA201170104A1 (ru) 2008-06-28 2009-06-29 Улучшенное получение и хранение белков в растениях

Country Status (20)

Country Link
US (1) US20100313307A1 (ru)
EP (1) EP2307547A4 (ru)
JP (1) JP2011526155A (ru)
KR (1) KR20110044211A (ru)
CN (1) CN102137932A (ru)
AP (1) AP2011005557A0 (ru)
AR (1) AR072391A1 (ru)
AU (1) AU2009261943A1 (ru)
BR (1) BRPI0914824A2 (ru)
CA (1) CA2729375A1 (ru)
CL (1) CL2010001598A1 (ru)
CO (1) CO6341485A2 (ru)
CU (1) CU20100263A7 (ru)
DO (1) DOP2010000399A (ru)
EA (1) EA201170104A1 (ru)
EC (1) ECSP11010793A (ru)
IL (1) IL210210A0 (ru)
MX (1) MX2010014541A (ru)
PE (1) PE20110562A1 (ru)
WO (2) WO2009158694A1 (ru)

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US8741591B2 (en) 2009-10-09 2014-06-03 The Research Foundation For The State University Of New York pH-insensitive glucose indicator protein
CN103221538B (zh) * 2010-10-01 2016-06-22 诺维信股份有限公司 β-葡糖苷酶变体及其编码多核苷酸
JP5809810B2 (ja) * 2011-02-22 2015-11-11 本田技研工業株式会社 タンパク質の植物細胞内への蓄積方法
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JP2015533293A (ja) * 2012-10-31 2015-11-24 ダニスコ・ユーエス・インク 組成物及び使用方法
KR101449155B1 (ko) * 2012-12-06 2014-10-13 주식회사 바이오앱 식물체에서 목적 단백질의 번역 효율을 증진시키기 위한 염기서열
CN106480089A (zh) * 2016-12-30 2017-03-08 上海交通大学 一种提高大豆含硫氨基酸含量及降低过敏原蛋白的方法
CN114634559A (zh) * 2018-10-12 2022-06-17 武汉禾元生物科技股份有限公司 一种提高胚乳生物反应器中重组蛋白表达水平的方法
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CN110122333A (zh) * 2019-06-17 2019-08-16 西安同人五凤农业有限公司 一种凤凰木种子促进发芽生根的方法
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Also Published As

Publication number Publication date
EP2307547A1 (en) 2011-04-13
DOP2010000399A (es) 2012-11-15
CN102137932A (zh) 2011-07-27
MX2010014541A (es) 2011-07-29
WO2009158694A1 (en) 2009-12-30
IL210210A0 (en) 2011-03-31
CU20100263A7 (es) 2012-06-21
PE20110562A1 (es) 2011-08-11
CO6341485A2 (es) 2011-11-21
US20100313307A1 (en) 2010-12-09
KR20110044211A (ko) 2011-04-28
CA2729375A1 (en) 2009-12-30
AR072391A1 (es) 2010-08-25
AP2011005557A0 (en) 2011-02-28
AU2009261943A1 (en) 2009-12-30
ECSP11010793A (es) 2011-07-29
JP2011526155A (ja) 2011-10-06
BRPI0914824A2 (pt) 2015-12-01
EP2307547A4 (en) 2011-06-22
WO2009158716A1 (en) 2009-12-30
CL2010001598A1 (es) 2011-07-15

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