AR072391A1 - Produccion de proteina heterologa y almacenamiento en semillas - Google Patents

Produccion de proteina heterologa y almacenamiento en semillas

Info

Publication number
AR072391A1
AR072391A1 ARP090102400A ARP090102400A AR072391A1 AR 072391 A1 AR072391 A1 AR 072391A1 AR P090102400 A ARP090102400 A AR P090102400A AR P090102400 A ARP090102400 A AR P090102400A AR 072391 A1 AR072391 A1 AR 072391A1
Authority
AR
Argentina
Prior art keywords
seed
protein
plant
seed storage
heterologous
Prior art date
Application number
ARP090102400A
Other languages
English (en)
Inventor
Monica S Schmidt
Eliot M Herman
Original Assignee
Donald Danforth Plant Science
Us Agriculture
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Donald Danforth Plant Science, Us Agriculture filed Critical Donald Danforth Plant Science
Publication of AR072391A1 publication Critical patent/AR072391A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/823—Reproductive tissue-specific promoters
    • C12N15/8234—Seed-specific, e.g. embryo, endosperm
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C12N15/8258—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Nutrition Science (AREA)
  • Developmental Biology & Embryology (AREA)
  • Pregnancy & Childbirth (AREA)
  • Reproductive Health (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Además se proveen métodos para producir una proteína heterologa en una semilla de planta y composicion enzimática. Reivindicacion 1: Una planta dicotiledonea transgénica caracterizado porque comprende: a) una deficiencia de una o más proteínas de almacenamiento de semillas, donde la deficiencia da por resultado al menos un 50% de reduccion en proteína de almacenamiento de semillas endogena en comparacion con la de una planta de tipo salvaje; y b) un polinucleotido heterologo que comprende un promotor de proteína de almacenamiento de semillas, un marco de lectura abierto que comprende una secuencia senal de ER, una secuencia codificadora de proteína deseada, y una senal de retencion de ER; donde el marco de lectura abierta está operativamente enlazado al promotor de la proteína de almacenamiento de semillas; y donde la semilla de la planta transgénica es capaz de producir una proteína heterologa a un nivel que es superior al 5% del peso seco total de la semilla.
ARP090102400A 2008-06-28 2009-06-29 Produccion de proteina heterologa y almacenamiento en semillas AR072391A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US7661608P 2008-06-28 2008-06-28

Publications (1)

Publication Number Publication Date
AR072391A1 true AR072391A1 (es) 2010-08-25

Family

ID=41444990

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP090102400A AR072391A1 (es) 2008-06-28 2009-06-29 Produccion de proteina heterologa y almacenamiento en semillas

Country Status (20)

Country Link
US (1) US20100313307A1 (es)
EP (1) EP2307547A4 (es)
JP (1) JP2011526155A (es)
KR (1) KR20110044211A (es)
CN (1) CN102137932A (es)
AP (1) AP2011005557A0 (es)
AR (1) AR072391A1 (es)
AU (1) AU2009261943A1 (es)
BR (1) BRPI0914824A2 (es)
CA (1) CA2729375A1 (es)
CL (1) CL2010001598A1 (es)
CO (1) CO6341485A2 (es)
CU (1) CU20100263A7 (es)
DO (1) DOP2010000399A (es)
EA (1) EA201170104A1 (es)
EC (1) ECSP11010793A (es)
IL (1) IL210210A0 (es)
MX (1) MX2010014541A (es)
PE (1) PE20110562A1 (es)
WO (2) WO2009158694A1 (es)

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DK2622069T3 (en) 2010-10-01 2016-02-22 Novozymes Inc Beta-glucosidasevarianter and polynucleotides encoding them
JP5809810B2 (ja) * 2011-02-22 2015-11-11 本田技研工業株式会社 タンパク質の植物細胞内への蓄積方法
WO2013149801A1 (en) * 2012-04-05 2013-10-10 Basf Plant Science Company Gmbh Fungal resistant plants expressing hydrophobin
MX2015005425A (es) * 2012-10-31 2015-08-05 Danisco Inc Composiciones y metodos de uso.
KR101449155B1 (ko) * 2012-12-06 2014-10-13 주식회사 바이오앱 식물체에서 목적 단백질의 번역 효율을 증진시키기 위한 염기서열
CN106480089A (zh) * 2016-12-30 2017-03-08 上海交通大学 一种提高大豆含硫氨基酸含量及降低过敏原蛋白的方法
CN114634559A (zh) * 2018-10-12 2022-06-17 武汉禾元生物科技股份有限公司 一种提高胚乳生物反应器中重组蛋白表达水平的方法
CN109220805A (zh) * 2018-11-05 2019-01-18 贵州大学 一种红豆树组培苗瓶外生根方法
IL265841A (en) * 2019-04-03 2020-10-28 Yeda Res & Dev A plant that expresses animal milk proteins
KR102213745B1 (ko) 2019-04-16 2021-02-09 주식회사 바이오앱 돼지 유행성 설사병 바이러스 백신 조성물 및 이의 제조 방법
CN110122333A (zh) * 2019-06-17 2019-08-16 西安同人五凤农业有限公司 一种凤凰木种子促进发芽生根的方法
WO2020256372A1 (ko) 2019-06-17 2020-12-24 주식회사 바이오앱 아프리카 돼지열병의 진단을 위한 항원 생산용 재조합 벡터 및 이의 용도
US11326176B2 (en) * 2019-11-22 2022-05-10 Mozza Foods, Inc. Recombinant micelle and method of in vivo assembly
CA3198652A1 (en) * 2020-10-28 2022-05-05 Hyeon-Je Cho Leghemoglobin in soybean
WO2022093977A1 (en) * 2020-10-30 2022-05-05 Fortiphyte, Inc. Pathogen resistance in plants
KR102630105B1 (ko) * 2020-11-26 2024-01-29 전남대학교산학협력단 Cel12A 단백질을 포함하는 셀룰로오스 분해용 조성물 및 이의 제조방법
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2023027402A1 (ko) 2021-08-27 2023-03-02 주식회사 바이오앱 아프리카 돼지열병 바이러스 유래 항원 단백질을 포함하는 아프리카 돼지열병의 예방용 백신
WO2023076272A1 (en) * 2021-10-25 2023-05-04 The Regents Of The University Of California Vectors and methods for improved dicot plant transformation frequency

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Also Published As

Publication number Publication date
JP2011526155A (ja) 2011-10-06
WO2009158694A1 (en) 2009-12-30
CO6341485A2 (es) 2011-11-21
CL2010001598A1 (es) 2011-07-15
CN102137932A (zh) 2011-07-27
PE20110562A1 (es) 2011-08-11
CA2729375A1 (en) 2009-12-30
MX2010014541A (es) 2011-07-29
WO2009158716A1 (en) 2009-12-30
BRPI0914824A2 (pt) 2015-12-01
CU20100263A7 (es) 2012-06-21
ECSP11010793A (es) 2011-07-29
IL210210A0 (en) 2011-03-31
DOP2010000399A (es) 2012-11-15
AU2009261943A1 (en) 2009-12-30
US20100313307A1 (en) 2010-12-09
EA201170104A1 (ru) 2011-08-30
EP2307547A1 (en) 2011-04-13
KR20110044211A (ko) 2011-04-28
AP2011005557A0 (en) 2011-02-28
EP2307547A4 (en) 2011-06-22

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