EA201001198A1 - Рекомбинантные вирусоподобные частицы (вч), схожие с вирусом гриппа, производящиеся в трансгенных растениях, экспрессирующих гемагглютинин - Google Patents

Рекомбинантные вирусоподобные частицы (вч), схожие с вирусом гриппа, производящиеся в трансгенных растениях, экспрессирующих гемагглютинин

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Publication number
EA201001198A1
EA201001198A1 EA201001198A EA201001198A EA201001198A1 EA 201001198 A1 EA201001198 A1 EA 201001198A1 EA 201001198 A EA201001198 A EA 201001198A EA 201001198 A EA201001198 A EA 201001198A EA 201001198 A1 EA201001198 A1 EA 201001198A1
Authority
EA
Eurasian Patent Office
Prior art keywords
virus
influenza virus
particles
produced
transgenic plants
Prior art date
Application number
EA201001198A
Other languages
English (en)
Other versions
EA034733B1 (ru
Inventor
Марк-Андре Д`Ау
Манон КУТЮР
Фредерик Орс
Сонья Трепанье
Пьер-Оливье Лавуа
Мишель Даргис
Луи-Филипп ВЕЗИНА
Натали ЛАНДРИ
Original Assignee
Медикаго Инк.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from CA002615372A external-priority patent/CA2615372A1/en
Application filed by Медикаго Инк. filed Critical Медикаго Инк.
Priority claimed from PCT/CA2009/000032 external-priority patent/WO2009076778A1/en
Publication of EA201001198A1 publication Critical patent/EA201001198A1/ru
Publication of EA034733B1 publication Critical patent/EA034733B1/ru

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A—HUMAN NECESSITIES
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Abstract

Предложен способ синтеза вирусоподобных частиц гриппа (ВЧ) в растениях или в частях растений. Способ включает экспрессию вируса гриппа (НА) в растениях и очистку с помощью эксклюзионной хроматографии. Изобретение также относится к получению ВЧ, содержащих белок НА вируса гриппа и липиды растительного происхождения. Раскрыты также нуклеиновая кислота, кодирующая белок вируса гриппа (НА), а также векторы. ВЧ могут быть использованы для создания вакцин или для увеличения эффективности уже существующих вакцин.
EA201001198A 2008-01-21 2009-01-12 Нуклеиновая кислота для увеличенной экспрессии гемагглютинина вируса гриппа в растении и ее применение EA034733B1 (ru)

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CA002615372A CA2615372A1 (en) 2007-07-13 2008-01-21 Influenza virus-like particles (vlps) comprising hemagglutinin
US2277508P 2008-01-22 2008-01-22
PCT/CA2008/001281 WO2009009876A1 (en) 2007-07-13 2008-07-11 Influenza virus-like particles (vlps) comprising hemagglutinin produced within a plant
PCT/CA2009/000032 WO2009076778A1 (en) 2007-11-27 2009-01-12 Recombinant influenza virus-like particles (vlps) produced in transgenic plants expressing hemagglutinin
CA2730185A CA2730185C (en) 2008-07-11 2009-07-02 Influenza virus-like particles (vlps) comprising hemagglutinin

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9815873B2 (en) 2011-03-23 2017-11-14 Medicago Inc. Method for recovering plant-derived proteins
US11826419B2 (en) 2009-09-22 2023-11-28 Medicago Inc. Method of preparing plant-derived VLPs

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2615372A1 (en) 2007-07-13 2009-01-13 Marc-Andre D'aoust Influenza virus-like particles (vlps) comprising hemagglutinin
EP2610345B1 (en) 2007-11-27 2015-08-19 Medicago Inc. Recombinant influenza virus-like particles (VLPS) produced in transgenic plants expressing hemagglutinin
US20110081367A1 (en) * 2008-06-14 2011-04-07 Veredus Laboratories Pte Ltd Influenza sequences
EP2294202B1 (en) 2008-07-08 2015-05-20 Medicago Inc. Soluble recombinant influenza antigens
PT2318530T (pt) 2008-07-18 2016-09-30 Medicago Inc Novo epítopo de imunização contra o vírus da gripe
US10272148B2 (en) 2009-06-24 2019-04-30 Medicago Inc. Chimeric influenza virus-like particles comprising hemagglutinin
RU2571223C2 (ru) * 2009-09-18 2015-12-20 Фронхофер Юэсэй Инк. Вирусоподобные частицы, содержащие белки-мишени, слитые с белками оболочки растительных вирусов
CN103282501A (zh) * 2010-11-04 2013-09-04 麦迪卡格公司 植物表达系统
TWI526539B (zh) 2010-12-22 2016-03-21 苜蓿股份有限公司 植物中生產類病毒顆粒(vlp)的方法及以該方法生產之vlp
JP6113155B2 (ja) * 2011-06-20 2017-04-12 ユニバーシティ オブ ピッツバーグ − オブ ザ コモンウェルス システム オブ ハイヤー エデュケイション 計算で最適化した広い反応性を示すｈ１ｎ１インフルエンザの抗原
DK3418300T3 (da) 2011-07-18 2020-12-07 Inst Res Biomedicine Neutralisering af anti-influenza-a-virusantistoffer og anvendelser deraf
US11390878B2 (en) 2011-09-30 2022-07-19 Medicago Inc. Increasing protein yield in plants
PT2760882T (pt) 2011-09-30 2023-08-07 Medicago Inc Aumento do rendimento de partícula semelhante a vírus em plantas
CA2855252C (en) * 2011-11-11 2023-03-07 Philip Morris Products S.A. Influenza virus-like particles (vlps) comprising hemagglutinin produced in nicotiana tabacum
WO2013119683A1 (en) * 2012-02-07 2013-08-15 University Of Pittsburgh - Of The Commonwealth System Of Higher Education Computationally optimized broadly reactive antigens for h3n2, h2n2, and b influenza viruses
RU2639551C2 (ru) 2012-03-30 2017-12-21 Юниверсити Оф Питтсбург - Оф Зе Коммонвэлс Систем Оф Хайе Эдьюкейшн Оптимизированные с помощью вычислительных средств антигены с широким спектром реактивности для вирусов гриппа h5n1 и h1n1
KR102134815B1 (ko) 2012-09-05 2020-07-17 메디카고 인코포레이티드 식물에서 피코르나바이러스-유사 입자 생산
US9309290B2 (en) 2012-11-27 2016-04-12 University of Pittsburgh—of the Commonwealth System of Higher Education Computationally optimized broadly reactive antigens for H1N1 influenza
CN112592389A (zh) 2013-03-28 2021-04-02 莫迪卡戈公司 植物中流感样病毒颗粒的产生
CN105980561B (zh) * 2014-01-10 2020-06-02 莫迪卡戈公司 Cpmv增强子元件
US10563213B2 (en) 2014-03-27 2020-02-18 Medicago Inc. Modified CPMV enhancer elements
EP3167057B1 (en) 2014-07-11 2020-03-25 Medicago Inc. Modifying protein production in plants
WO2016168187A1 (en) * 2015-04-13 2016-10-20 The Regents Of The University Of Michigan Virus-like particles
WO2016196846A2 (en) * 2015-06-02 2016-12-08 Sanofi Pasteur Inc. Engineered influenza antigenic polypeptides and immunogenic compositions thereof
KR20180088629A (ko) 2015-07-02 2018-08-06 메디카고 인코포레이티드 자스몬산 경로 활성화제
US10844097B2 (en) 2016-06-02 2020-11-24 Sanofi Pasteur Inc. Engineered influenza antigenic polypeptides and immunogenic compositions thereof
JP6622825B2 (ja) * 2018-01-25 2019-12-18 インスティテュート・フォー・リサーチ・イン・バイオメディシンＩｎｓｔｉｔｕｔｅ Ｆｏｒ Ｒｅｓｅａｒｃｈ Ｉｎ Ｂｉｏｍｅｄｉｃｉｎｅ Ａ型インフルエンザウイルス中和抗体及びその使用法
EP3814507A4 (en) * 2018-06-27 2022-07-06 Medicago Inc. INFLUENZA VIRUS HEMAGGLUTININ MUTANTS
WO2020092207A1 (en) * 2018-10-28 2020-05-07 University Of Georgia Research Foundation Broadly reactive immunogens of influenza virus, compositions, and methods of use thereof
EP3935180A4 (en) * 2019-03-06 2022-12-28 Plantform Corporation T-DNA VECTORS WITH ENGINEERED 5' SEQUENCES PRE-POST-TRANSLATIONAL MODIFICATION ENZYMES AND METHODS OF USE THEREOF
EP3938511A4 (en) 2019-03-14 2023-02-08 Mitsubishi Tanabe Pharma Corporation ENDOGENOUS PLANT EXPRESSION ACTIVATOR
JP2020048568A (ja) * 2019-11-22 2020-04-02 インスティテュート・フォー・リサーチ・イン・バイオメディシンＩｎｓｔｉｔｕｔｅ Ｆｏｒ Ｒｅｓｅａｒｃｈ Ｉｎ Ｂｉｏｍｅｄｉｃｉｎｅ Ａ型インフルエンザウイルス中和抗体及びその使用法
US20230203101A1 (en) * 2020-04-22 2023-06-29 POSTECH Research and Business Development Foundation Coronavirus Disease 2019(COVID -19) Recombinant Spike Protein Forming Trimer, Method for Mass Producing Recombinant Spike Protein in Plants, and Method for Preparing Vaccine Composition on Basis Thereof
CN117479831A (zh) 2021-06-09 2024-01-30 南特知识产权控股有限责任公司 用于在植物中生产感兴趣的蛋白质的方法和系统
CN114891074B (zh) * 2022-05-10 2023-04-11 中山大学·深圳 一种季节性甲型流感通用病毒样颗粒及其制备方法与应用
CN115992101B (zh) * 2023-03-22 2023-07-28 深圳市卫光生物制品股份有限公司 一种流感病毒裂解疫苗原液的制备方法

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5428147A (en) 1983-04-15 1995-06-27 Mycogen Plant Science, Inc. Octopine T-DNA promoters
US5036006A (en) 1984-11-13 1991-07-30 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5100792A (en) 1984-11-13 1992-03-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues
US4962028A (en) 1986-07-09 1990-10-09 Dna Plant Technology Corporation Plant promotors
US5232833A (en) 1988-09-14 1993-08-03 Stressgen Biotechnologies Corporation Accumulation of heat shock proteins for evaluating biological damage due to chronic exposure of an organism to sublethal levels of pollutants
US6403865B1 (en) 1990-08-24 2002-06-11 Syngenta Investment Corp. Method of producing transgenic maize using direct transformation of commercially important genotypes
UA48104C2 (ru) 1991-10-04 2002-08-15 Новартіс Аг Фрагмент днк, содержащий последовательность, которая кодирует инсектицидный протеин, оптимизированную для кукурузы, фрагмент днк, обеспечивающий направленную желательную для сердцевины стебля экспрессию связанного с ней структурного гена в растении, фрагмент днк, обеспечивающий специфическую для пыльцы экспрессию связанного с ней структурного гена в растении, рекомбинантная молекула днк, способ получения оптимизированной для кукурузы кодирующей последовательности инсектицидного протеина, способ защиты растений кукурузы по меньшей мере от одного насекомого-вредителя
US7125978B1 (en) 1999-10-04 2006-10-24 Medicago Inc. Promoter for regulating expression of foreign genes
DE60022369T2 (de) 1999-10-04 2006-05-18 Medicago Inc., Sainte Foy Verfahren zur regulation der transkription von fremden genen in gegenwart von stickstoff
WO2002000885A2 (en) * 2000-06-23 2002-01-03 American Cyanamid Company Assembly of wild-type and chimeric influenza virus-like particles (vlps)
BRPI0410340A (pt) * 2003-05-05 2006-05-30 Dow Agrosciences Llc vetores e células para preparar composições imunoprotetoras derivadas de plantas transgênicas
EP1635772A4 (en) 2003-05-05 2008-02-13 Dow Agrosciences Llc STERILE IMMUNOPROPHYLACTIC AND THERAPEUTIC COMPOSITIONS DERIVED FROM TRANSGENIC VEGETABLE CELLS AND METHODS OF PRODUCTION THEREOF
US8592197B2 (en) * 2003-07-11 2013-11-26 Novavax, Inc. Functional influenza virus-like particles (VLPs)
CN100410378C (zh) * 2005-05-09 2008-08-13 中国农业科学院生物技术研究所 编码禽流感血凝素的基因及其植物表达载体和应用
BRPI0613625A2 (pt) * 2005-07-19 2011-01-18 Dow Global Technologies Inc vacinas da gripe recombinantes
WO2008060669A2 (en) * 2006-04-21 2008-05-22 Dow Agrosciences Llc Vaccine for avian influenza and methods of use
US8778353B2 (en) * 2006-05-01 2014-07-15 Technovax, Inc. Influenza virus-like particle (VLP) compositions
WO2007135480A1 (en) 2006-05-22 2007-11-29 Plant Bioscience Limited Bipartite system, method and composition for the constitutive and inducible expression of high levels of foreign proteins in plants
KR100964462B1 (ko) * 2007-07-10 2010-06-16 성균관대학교산학협력단 형질전환 식물 유래의 조류독감 바이러스 백신 및 그 제조방법

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11826419B2 (en) 2009-09-22 2023-11-28 Medicago Inc. Method of preparing plant-derived VLPs
US11833200B2 (en) 2009-09-22 2023-12-05 Medicago Inc. Method of preparing plant-derived proteins
US9815873B2 (en) 2011-03-23 2017-11-14 Medicago Inc. Method for recovering plant-derived proteins

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