EA200600952A1 - Способ культивирования микроорганизмов рода thraustochytriales - Google Patents

Способ культивирования микроорганизмов рода thraustochytriales

Info

Publication number
EA200600952A1
EA200600952A1 EA200600952A EA200600952A EA200600952A1 EA 200600952 A1 EA200600952 A1 EA 200600952A1 EA 200600952 A EA200600952 A EA 200600952A EA 200600952 A EA200600952 A EA 200600952A EA 200600952 A1 EA200600952 A1 EA 200600952A1
Authority
EA
Eurasian Patent Office
Prior art keywords
caco
microorganisms
thraustochytriales
pufa
increased
Prior art date
Application number
EA200600952A
Other languages
English (en)
Other versions
EA011877B1 (ru
Inventor
Маркус Луи
Маттиас Рюзинг
Original Assignee
Нутринова Ньютришн Спешиэлтис Энд Фуд Ингридиентс Гмбх
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Нутринова Ньютришн Спешиэлтис Энд Фуд Ингридиентс Гмбх filed Critical Нутринова Ньютришн Спешиэлтис Энд Фуд Ингридиентс Гмбх
Publication of EA200600952A1 publication Critical patent/EA200600952A1/ru
Publication of EA011877B1 publication Critical patent/EA011877B1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Fodder In General (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Edible Oils And Fats (AREA)

Abstract

Данное изобретение относится к оптимизированному способу получения PUFA культивированием микроорганизмов отряда Thraustochytriales в ферментационной среде со стабилизированным при помощи СаСОзначением рН, включающей в себя 3-15 г/л СаСО, и последующим выделением PUFA из этих микроорганизмов и/или этой среды. В частности, описаны новые оптимизированные среды с различным содержанием СаСО. Применение соответствующих количеств СаСОделает возможным значительное упрощение проведения процесса во время ферментации. Кроме того, могут быть получены повышенные количества DHA при повышенном содержании масла в биомассе. Эти повышенные количества делают возможной ферментацию микроорганизмов Thraustochytriales без регулирования рН и тем самым позволяют значительно улучшить и значительно упростить получение PUFA.
EA200600952A 2003-11-10 2004-11-10 Способ культивирования микроорганизмов рода thraustochytriales EA011877B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10352837A DE10352837A1 (de) 2003-11-10 2003-11-10 Prozess zur Kultivierung von Mikroorganismen der Gattung Thraustochytriales
PCT/EP2004/012715 WO2005045050A1 (de) 2003-11-10 2004-11-10 Prozess zur kultivierung von mikroorganismen der gattung thraustochytrieales

Publications (2)

Publication Number Publication Date
EA200600952A1 true EA200600952A1 (ru) 2007-02-27
EA011877B1 EA011877B1 (ru) 2009-06-30

Family

ID=34559599

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200600952A EA011877B1 (ru) 2003-11-10 2004-11-10 Способ культивирования микроорганизмов рода thraustochytriales

Country Status (17)

Country Link
US (1) US8889382B2 (ru)
EP (1) EP1685255B1 (ru)
JP (1) JP4791366B2 (ru)
KR (1) KR101159869B1 (ru)
CN (2) CN104195186A (ru)
AT (1) ATE556143T1 (ru)
AU (1) AU2004287953B2 (ru)
BR (1) BRPI0416362A (ru)
CA (1) CA2545416C (ru)
DE (1) DE10352837A1 (ru)
EA (1) EA011877B1 (ru)
ES (1) ES2387305T3 (ru)
IL (1) IL175521A (ru)
PL (1) PL1685255T3 (ru)
SG (1) SG148156A1 (ru)
WO (1) WO2005045050A1 (ru)
ZA (1) ZA200603855B (ru)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2066780A4 (en) * 2006-09-18 2013-02-27 Univ Arizona ALGACED FATTY ACIDS WITH MEDIUM CHAIN LENGTH AND HYDROCARBONS ALGACES
ITMI20061843A1 (it) * 2006-09-27 2008-03-28 Immobiliare G M S R L Formulazioni di medium colturali idonei alla applicazione industriale
US20100086979A1 (en) * 2006-10-27 2010-04-08 Thomas Kiy Production of omega-3 fatty acids in microflora of thraustochytriales using modified media
JP5219390B2 (ja) * 2007-03-19 2013-06-26 学校法人立命館 微生物培養用培地及び微生物製剤
EP2071019A1 (en) * 2007-12-15 2009-06-17 Lonza AG Method for the cultivation of microoranisms of the order thraustochytriales
JP5709196B2 (ja) * 2009-01-09 2015-04-30 国立大学法人山梨大学 油脂生産菌の培養方法
WO2010097809A2 (en) * 2009-02-25 2010-09-02 V.B. Medicare Pvt. Ltd. Improved methods for fermentative production of docosahexaenoic acid
CN103649313B (zh) * 2011-03-07 2017-10-24 Dsm营养产品股份公司 工程化破囊壶菌属微生物
MX344443B (es) * 2011-07-13 2016-12-15 Alltech Inc Composiciones de lipidos algales y metodos para prepararlas y utilizarlas.
CN102911868B (zh) * 2012-09-28 2015-12-09 新奥科技发展有限公司 一种微生物培养基及培养方法
CN106795539B (zh) * 2014-05-22 2021-06-22 玛拉可再生能源公司 在微生物中产生油的方法
CZ307271B6 (cs) * 2014-08-26 2018-05-09 Vysoká škola chemicko - technologická v Praze Způsob kultivace mořských protistů, zejména mikroorganismů rodu Thraustochytriales, na médiu obsahujícím odpadní solný roztok z demineralizace sladké mléčné syrovátky

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0113183A1 (en) * 1982-12-01 1984-07-11 Thomas Anthony Carson Fermentation method and composition for use therein
JPH01247079A (ja) * 1988-03-28 1989-10-02 Nec Corp 培地のｐＨ調整方法
US5407957A (en) 1990-02-13 1995-04-18 Martek Corporation Production of docosahexaenoic acid by dinoflagellates
JPH08196288A (ja) * 1995-01-30 1996-08-06 Kaiyo Bio Technol Kenkyusho:Kk 長鎖脂肪酸の製造法
JP2764572B2 (ja) * 1995-04-17 1998-06-11 工業技術院長 ドコサヘキサエン酸生産能を有する新規微生物及びそれを用いたドコサヘキサエン酸の製造方法
EP0823475B1 (en) * 1995-04-17 2009-06-17 National Institute of Advanced Industrial Science and Technology Novel microorganisms capable of producing highly unsaturated fatty acids and process for producing highly unsaturated fatty acids by using the microorganisms
EE04063B1 (et) * 1996-07-23 2003-06-16 Nagase Biochemicals, Ltd. Meetod dokosaheksaeenhappe ning dokosapentaeenhappe valmistamiseks

Also Published As

Publication number Publication date
SG148156A1 (en) 2008-12-31
AU2004287953B2 (en) 2010-04-29
AU2004287953A1 (en) 2005-05-19
ES2387305T3 (es) 2012-09-20
IL175521A (en) 2011-12-29
ZA200603855B (en) 2007-10-31
EA011877B1 (ru) 2009-06-30
ATE556143T1 (de) 2012-05-15
BRPI0416362A (pt) 2007-03-13
US8889382B2 (en) 2014-11-18
CN1914327A (zh) 2007-02-14
WO2005045050A1 (de) 2005-05-19
KR101159869B1 (ko) 2012-06-25
IL175521A0 (en) 2006-09-05
JP4791366B2 (ja) 2011-10-12
DE10352837A1 (de) 2005-07-07
JP2007512809A (ja) 2007-05-24
PL1685255T3 (pl) 2012-12-31
EP1685255A1 (de) 2006-08-02
CN104195186A (zh) 2014-12-10
CA2545416C (en) 2014-02-25
KR20070042114A (ko) 2007-04-20
CA2545416A1 (en) 2005-05-19
EP1685255B1 (de) 2012-05-02
US20070141686A1 (en) 2007-06-21

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Legal Events

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MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): AM AZ BY KZ KG MD TJ TM

MM4A Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s)

Designated state(s): RU