EA200600947A1 - Способ культивирования микроорганизмов рода thraustochytriales с использованием оптимизированной низкосолевой среды - Google Patents

Способ культивирования микроорганизмов рода thraustochytriales с использованием оптимизированной низкосолевой среды

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Publication number
EA200600947A1
EA200600947A1 EA200600947A EA200600947A EA200600947A1 EA 200600947 A1 EA200600947 A1 EA 200600947A1 EA 200600947 A EA200600947 A EA 200600947A EA 200600947 A EA200600947 A EA 200600947A EA 200600947 A1 EA200600947 A1 EA 200600947A1
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Eurasian Patent Office
Prior art keywords
microorganisms
medium
salt
cultivation
salts
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EA200600947A
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English (en)
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EA011858B1 (ru
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Маттиас Рюзинг
Маркус Луи
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Нутринова Ньютришн Спешиэлтис Энд Фуд Ингридиентс Гмбх
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Publication of EA200600947A1 publication Critical patent/EA200600947A1/ru
Publication of EA011858B1 publication Critical patent/EA011858B1/ru

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6434—Docosahexenoic acids [DHA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/115—Fatty acids or derivatives thereof; Fats or oils
    • A23L33/12—Fatty acids or derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6436—Fatty acid esters
    • C12P7/6445—Glycerides
    • C12P7/6472—Glycerides containing polyunsaturated fatty acid [PUFA] residues, i.e. having two or more double bonds in their backbone
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Polymers & Plastics (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Food Science & Technology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Physiology (AREA)
  • Animal Husbandry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Fodder In General (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Edible Oils And Fats (AREA)

Abstract

Данное изобретение относится к оптимизированному способу культивирования микроорганизмов рода Thraustochytriales, в соответствии с которым эти микроорганизмы культивируют в низкосолевой среде без добавления солей натрия и хлоридных солей при общем содержании соли менее чем 3,5 г/л (соответственно менее чем 10% содержания соли морской воды) с последующим выделением PUFA из этих микроорганизмов и/или из этой среды. В частности, описаны новые оптимизированные среды с существенно уменьшенным общим содержанием соли при особенно уменьшенном содержании NaCl. Новая комбинация различных солей в качестве состава сред с суммой массовых долей Na- и Cl-ионов не более чем 1,75 г/л позволяет значительно улучшить и значительно упростить получение PUFA. Кроме того, описанная среда предпочтительно вообще не содержит добавленных солей натрия и хлоридных солей, что помогает избежать загрязнений окружающей среды содержащими соль сточными водами.
EA200600947A 2003-11-10 2004-11-10 Способ культивирования микроорганизмов рода thraustochytriales с использованием оптимизированной низкосолевой среды EA011858B1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE2003152838 DE10352838A1 (de) 2003-11-10 2003-11-10 Verfahren zur Kultivierung von Mikroorganismen der Gattung Thraustochytriales unter Verwendung eines optimierten Niedrigsalzmediums
PCT/EP2004/012718 WO2005045003A2 (de) 2003-11-10 2004-11-10 Verfahren zur kultivierung von mikroorganismen der gattung thraustochytriales unter verwendung eines optimierten niedrigsalzmediums

Publications (2)

Publication Number Publication Date
EA200600947A1 true EA200600947A1 (ru) 2007-02-27
EA011858B1 EA011858B1 (ru) 2009-06-30

Family

ID=34559600

Family Applications (1)

Application Number Title Priority Date Filing Date
EA200600947A EA011858B1 (ru) 2003-11-10 2004-11-10 Способ культивирования микроорганизмов рода thraustochytriales с использованием оптимизированной низкосолевой среды

Country Status (14)

Country Link
US (2) US8900831B2 (ru)
EP (2) EP1685241A2 (ru)
JP (1) JP4791367B2 (ru)
KR (1) KR101238613B1 (ru)
CN (2) CN1977038B (ru)
AU (1) AU2004287954B2 (ru)
BR (1) BRPI0416360A (ru)
CA (1) CA2545410A1 (ru)
DE (1) DE10352838A1 (ru)
EA (1) EA011858B1 (ru)
IL (1) IL175520A (ru)
SG (1) SG148157A1 (ru)
WO (1) WO2005045003A2 (ru)
ZA (1) ZA200603854B (ru)

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EP2105506A1 (de) * 2008-03-26 2009-09-30 Lonza Ag Verfahren zur Herstellung PUFAs enthaltender Öle unter Verwendung von Mikroorganismen der Ordnung Labyrinthulomycota
US8207363B2 (en) 2009-03-19 2012-06-26 Martek Biosciences Corporation Thraustochytrids, fatty acid compositions, and methods of making and uses thereof
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KR101134324B1 (ko) * 2011-10-07 2012-04-13 나윤경 미생물 대량 생산을 위한 분말 배지
KR101418066B1 (ko) * 2012-11-30 2014-07-09 롯데케미칼 주식회사 미세조류를 이용한 도코사헥사엔산 생산방법
EP2826384A1 (de) 2013-07-16 2015-01-21 Evonik Industries AG Verfahren zur Trocknung von Biomasse
FR3019559B1 (fr) * 2014-04-03 2018-01-05 Fermentalg Procede de culture des microalgues du genre aurantiochytrium dans un milieu de culture a teneur reduite en sodium et en chlorure pour la production de dha
KR20170005832A (ko) * 2014-05-08 2017-01-16 도널드 엠. 스미스 인간의 건강을 위한 오메가 3''s 함량이 높은 고기를 생산하기 위한 소 및 들소를 위한 사료 보충제로서 통 조류의 선택, 생산 및 급여
CN106795539B (zh) * 2014-05-22 2021-06-22 玛拉可再生能源公司 在微生物中产生油的方法
CN104004661B (zh) * 2014-05-22 2017-12-15 广东省生物工程研究所（广州甘蔗糖业研究所） 一种可提高鸡蛋品质的蛋鸡日粮
CN104277975A (zh) * 2014-09-28 2015-01-14 江苏科技大学 脱脂蚕蛹渣用于产油微生物培养的预处理方法及其产物和应用
WO2016050552A1 (de) 2014-10-02 2016-04-07 Evonik Degussa Gmbh Verfahren zur herstellung einer pufas enthaltenden biomasse mit hoher zellstabilität
BR112017006833B1 (pt) 2014-10-02 2022-09-13 Evonik Operations Gmbh Alimento para animais contendo ácido graxo poli-insaturado e processo para produzir o mesmo
ES2873094T3 (es) 2014-10-02 2021-11-03 Evonik Operations Gmbh Procedimiento para la producción de un pienso que contiene PUFAs mediante extrusión de una biomasa que contiene PUFAs de tipo Labyrinthulomycetes
BR112017005388B1 (pt) 2014-10-02 2022-09-13 Evonik Operations Gmbh Alimento para animais contendo biomassa de aurantiochytrium
JP6998935B2 (ja) 2016-07-13 2022-01-18 エボニック オペレーションズ ゲーエムベーハー 溶解された脂質含有バイオマスから脂質を分離する方法
KR102027632B1 (ko) * 2016-12-06 2019-10-01 국립해양생물자원관 고온성 미생물 배양용 고체배지조성물 및 그 제조방법
DK3562925T3 (da) 2016-12-27 2021-05-25 Evonik Operations Gmbh Fremgangsmåde til isolering af lipider fra en lipidholdig biomasse
EP3527664A1 (en) 2018-02-15 2019-08-21 Evonik Degussa GmbH Method of isolating lipids from a lipids containing biomass
BR112020023222A2 (pt) 2018-05-15 2021-03-23 Evonik Operations Gmbh método de isolamento de lipídios a partir de uma biomassa contendo lipídios lisados por inversão por emulsão
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Also Published As

Publication number Publication date
EA011858B1 (ru) 2009-06-30
AU2004287954A1 (en) 2005-05-19
KR20070042115A (ko) 2007-04-20
CN104073442A (zh) 2014-10-01
JP2007510422A (ja) 2007-04-26
WO2005045003A3 (de) 2005-08-04
CA2545410A1 (en) 2005-05-19
EP2816104A1 (de) 2014-12-24
WO2005045003A2 (de) 2005-05-19
CN1977038B (zh) 2014-12-24
JP4791367B2 (ja) 2011-10-12
SG148157A1 (en) 2008-12-31
US20150104557A1 (en) 2015-04-16
ZA200603854B (en) 2007-11-28
IL175520A0 (en) 2006-09-05
KR101238613B1 (ko) 2013-02-28
US20070054384A1 (en) 2007-03-08
EP1685241A2 (de) 2006-08-02
CN1977038A (zh) 2007-06-06
IL175520A (en) 2014-08-31
AU2004287954B2 (en) 2010-04-29
US8900831B2 (en) 2014-12-02
DE10352838A1 (de) 2005-07-07
BRPI0416360A (pt) 2007-05-08

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