EA028164B1 - Слитые белки для облегчения отбора клеток, инфицированных рекомбинантным вирусом осповакцины с геном специфического иммуноглобулина - Google Patents
Слитые белки для облегчения отбора клеток, инфицированных рекомбинантным вирусом осповакцины с геном специфического иммуноглобулина Download PDFInfo
- Publication number
- EA028164B1 EA028164B1 EA201491965A EA201491965A EA028164B1 EA 028164 B1 EA028164 B1 EA 028164B1 EA 201491965 A EA201491965 A EA 201491965A EA 201491965 A EA201491965 A EA 201491965A EA 028164 B1 EA028164 B1 EA 028164B1
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- immunoglobulin
- library
- antigen
- virus
- eeu
- Prior art date
Links
- 241000700618 Vaccinia virus Species 0.000 title claims description 86
- 108020001507 fusion proteins Proteins 0.000 title claims description 64
- 102000037865 fusion proteins Human genes 0.000 title claims description 64
- 108700005091 Immunoglobulin Genes Proteins 0.000 title description 4
- 108060003951 Immunoglobulin Proteins 0.000 claims abstract description 160
- 102000018358 immunoglobulin Human genes 0.000 claims abstract description 160
- 239000000427 antigen Substances 0.000 claims abstract description 144
- 108091007433 antigens Proteins 0.000 claims abstract description 144
- 102000036639 antigens Human genes 0.000 claims abstract description 144
- 239000012634 fragment Substances 0.000 claims abstract description 124
- 238000000034 method Methods 0.000 claims abstract description 79
- 230000006798 recombination Effects 0.000 claims abstract description 9
- 238000005215 recombination Methods 0.000 claims abstract description 9
- 108091033319 polynucleotide Proteins 0.000 claims description 145
- 102000040430 polynucleotide Human genes 0.000 claims description 145
- 239000002157 polynucleotide Substances 0.000 claims description 145
- 241000700605 Viruses Species 0.000 claims description 141
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 132
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 124
- 229920001184 polypeptide Polymers 0.000 claims description 122
- 230000027455 binding Effects 0.000 claims description 65
- 102220542269 Eukaryotic peptide chain release factor GTP-binding subunit ERF3B_A56K_mutation Human genes 0.000 claims description 49
- 102000018697 Membrane Proteins Human genes 0.000 claims description 32
- 108010052285 Membrane Proteins Proteins 0.000 claims description 32
- 239000013598 vector Substances 0.000 claims description 28
- 239000012528 membrane Substances 0.000 claims description 27
- 229960005486 vaccine Drugs 0.000 claims description 27
- 229940083538 smallpox vaccine Drugs 0.000 claims description 26
- 150000001413 amino acids Chemical class 0.000 claims description 25
- 229940072221 immunoglobulins Drugs 0.000 claims description 23
- 102000006496 Immunoglobulin Heavy Chains Human genes 0.000 claims description 22
- 108010019476 Immunoglobulin Heavy Chains Proteins 0.000 claims description 22
- 102000013463 Immunoglobulin Light Chains Human genes 0.000 claims description 19
- 108010065825 Immunoglobulin Light Chains Proteins 0.000 claims description 19
- 230000004927 fusion Effects 0.000 claims description 18
- 230000003834 intracellular effect Effects 0.000 claims description 13
- 239000002245 particle Substances 0.000 claims description 13
- 239000013600 plasmid vector Substances 0.000 claims description 7
- 238000000605 extraction Methods 0.000 claims description 5
- 241000700647 Variola virus Species 0.000 claims description 4
- 229940023143 protein vaccine Drugs 0.000 claims 1
- 230000014509 gene expression Effects 0.000 abstract description 26
- 238000012216 screening Methods 0.000 abstract description 24
- 210000003527 eukaryotic cell Anatomy 0.000 abstract description 8
- 210000004027 cell Anatomy 0.000 description 234
- 239000011324 bead Substances 0.000 description 117
- 108090000623 proteins and genes Proteins 0.000 description 80
- 102000004169 proteins and genes Human genes 0.000 description 65
- 108020004414 DNA Proteins 0.000 description 42
- 238000010186 staining Methods 0.000 description 35
- 208000015181 infectious disease Diseases 0.000 description 34
- 210000002845 virion Anatomy 0.000 description 34
- 150000007523 nucleic acids Chemical group 0.000 description 32
- 238000004458 analytical method Methods 0.000 description 31
- 238000000684 flow cytometry Methods 0.000 description 31
- 102000039446 nucleic acids Human genes 0.000 description 31
- 108020004707 nucleic acids Proteins 0.000 description 31
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 29
- 206010046865 Vaccinia virus infection Diseases 0.000 description 29
- 239000000243 solution Substances 0.000 description 29
- 125000002088 tosyl group Chemical group [H]C1=C([H])C(=C([H])C([H])=C1C([H])([H])[H])S(*)(=O)=O 0.000 description 26
- 208000007089 vaccinia Diseases 0.000 description 26
- 239000000047 product Substances 0.000 description 19
- 108010076504 Protein Sorting Signals Proteins 0.000 description 18
- 102200091301 rs1114167429 Human genes 0.000 description 16
- 230000003321 amplification Effects 0.000 description 15
- 238000010790 dilution Methods 0.000 description 15
- 239000012895 dilution Substances 0.000 description 15
- 238000003199 nucleic acid amplification method Methods 0.000 description 15
- 238000002474 experimental method Methods 0.000 description 14
- 239000013612 plasmid Substances 0.000 description 14
- 239000006228 supernatant Substances 0.000 description 14
- 125000003275 alpha amino acid group Chemical group 0.000 description 13
- 239000002773 nucleotide Substances 0.000 description 13
- 125000003729 nucleotide group Chemical group 0.000 description 13
- 230000000692 anti-sense effect Effects 0.000 description 12
- 238000010367 cloning Methods 0.000 description 10
- 229960004927 neomycin Drugs 0.000 description 10
- 241000124008 Mammalia Species 0.000 description 9
- 241001465754 Metazoa Species 0.000 description 9
- 229930193140 Neomycin Natural products 0.000 description 9
- 229960000856 protein c Drugs 0.000 description 9
- 230000032258 transport Effects 0.000 description 9
- 101800004937 Protein C Proteins 0.000 description 8
- 102000017975 Protein C Human genes 0.000 description 8
- 101800001700 Saposin-D Proteins 0.000 description 8
- 238000003556 assay Methods 0.000 description 8
- 239000000872 buffer Substances 0.000 description 8
- ZCCUUQDIBDJBTK-UHFFFAOYSA-N psoralen Chemical compound C1=C2OC(=O)C=CC2=CC2=C1OC=C2 ZCCUUQDIBDJBTK-UHFFFAOYSA-N 0.000 description 8
- 239000006145 Eagle's minimal essential medium Substances 0.000 description 7
- 230000015572 biosynthetic process Effects 0.000 description 7
- 238000010276 construction Methods 0.000 description 7
- 238000013461 design Methods 0.000 description 7
- 238000001514 detection method Methods 0.000 description 7
- 239000000203 mixture Substances 0.000 description 7
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 7
- 239000000758 substrate Substances 0.000 description 7
- 230000003612 virological effect Effects 0.000 description 7
- 208000003322 Coinfection Diseases 0.000 description 6
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 6
- 239000002299 complementary DNA Substances 0.000 description 6
- 230000002458 infectious effect Effects 0.000 description 6
- 241000894007 species Species 0.000 description 6
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 5
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 5
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 5
- 230000000890 antigenic effect Effects 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 5
- 210000001185 bone marrow Anatomy 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 5
- 230000006870 function Effects 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 5
- 238000010187 selection method Methods 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- 238000013518 transcription Methods 0.000 description 5
- 230000035897 transcription Effects 0.000 description 5
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 4
- VXGRJERITKFWPL-UHFFFAOYSA-N 4',5'-Dihydropsoralen Natural products C1=C2OC(=O)C=CC2=CC2=C1OCC2 VXGRJERITKFWPL-UHFFFAOYSA-N 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 108010090804 Streptavidin Proteins 0.000 description 4
- 239000000654 additive Substances 0.000 description 4
- 230000000996 additive effect Effects 0.000 description 4
- 230000000295 complement effect Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- UGDGKPDPIXAUJL-UHFFFAOYSA-N ethyl n-[4-[benzyl(2-phenylethyl)amino]-2-(4-ethylphenyl)-1h-imidazo[4,5-c]pyridin-6-yl]carbamate Chemical compound N=1C(NC(=O)OCC)=CC=2NC(C=3C=CC(CC)=CC=3)=NC=2C=1N(CC=1C=CC=CC=1)CCC1=CC=CC=C1 UGDGKPDPIXAUJL-UHFFFAOYSA-N 0.000 description 4
- 239000013604 expression vector Substances 0.000 description 4
- 230000005764 inhibitory process Effects 0.000 description 4
- 239000003550 marker Substances 0.000 description 4
- 238000004091 panning Methods 0.000 description 4
- 230000008707 rearrangement Effects 0.000 description 4
- 230000001105 regulatory effect Effects 0.000 description 4
- 238000012163 sequencing technique Methods 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000000725 suspension Substances 0.000 description 4
- 238000004448 titration Methods 0.000 description 4
- 241000251468 Actinopterygii Species 0.000 description 3
- 241000271566 Aves Species 0.000 description 3
- 108091026890 Coding region Proteins 0.000 description 3
- 102000029749 Microtubule Human genes 0.000 description 3
- 108091022875 Microtubule Proteins 0.000 description 3
- 241000699670 Mus sp. Species 0.000 description 3
- 229930040373 Paraformaldehyde Natural products 0.000 description 3
- 230000003115 biocidal effect Effects 0.000 description 3
- 210000000170 cell membrane Anatomy 0.000 description 3
- 238000007796 conventional method Methods 0.000 description 3
- 210000000805 cytoplasm Anatomy 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000003623 enhancer Substances 0.000 description 3
- 210000004688 microtubule Anatomy 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- 229920002866 paraformaldehyde Polymers 0.000 description 3
- 230000037361 pathway Effects 0.000 description 3
- 230000010076 replication Effects 0.000 description 3
- 230000003248 secreting effect Effects 0.000 description 3
- 230000028327 secretion Effects 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 241000283707 Capra Species 0.000 description 2
- 241000282693 Cercopithecidae Species 0.000 description 2
- 108010047041 Complementarity Determining Regions Proteins 0.000 description 2
- 230000004543 DNA replication Effects 0.000 description 2
- 241000450599 DNA viruses Species 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 241000283086 Equidae Species 0.000 description 2
- 102000003886 Glycoproteins Human genes 0.000 description 2
- 108090000288 Glycoproteins Proteins 0.000 description 2
- 101710154606 Hemagglutinin Proteins 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 102000009786 Immunoglobulin Constant Regions Human genes 0.000 description 2
- 108010009817 Immunoglobulin Constant Regions Proteins 0.000 description 2
- 102000005431 Molecular Chaperones Human genes 0.000 description 2
- 108010006519 Molecular Chaperones Proteins 0.000 description 2
- 241000699666 Mus <mouse, genus> Species 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 2
- 101710093908 Outer capsid protein VP4 Proteins 0.000 description 2
- 101710135467 Outer capsid protein sigma-1 Proteins 0.000 description 2
- 241000288906 Primates Species 0.000 description 2
- 101710176177 Protein A56 Proteins 0.000 description 2
- 241000283984 Rodentia Species 0.000 description 2
- 102220469221 S-adenosyl-L-methionine-dependent tRNA 4-demethylwyosine synthase TYW1B_E18A_mutation Human genes 0.000 description 2
- 101150026794 SHO1 gene Proteins 0.000 description 2
- 125000000539 amino acid group Chemical group 0.000 description 2
- 238000012197 amplification kit Methods 0.000 description 2
- 210000000628 antibody-producing cell Anatomy 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 238000004113 cell culture Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 239000013078 crystal Substances 0.000 description 2
- 230000001086 cytosolic effect Effects 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 238000007429 general method Methods 0.000 description 2
- 210000004602 germ cell Anatomy 0.000 description 2
- 210000002288 golgi apparatus Anatomy 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 239000000185 hemagglutinin Substances 0.000 description 2
- 238000002744 homologous recombination Methods 0.000 description 2
- 230000006801 homologous recombination Effects 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 210000003519 mature b lymphocyte Anatomy 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000007758 minimum essential medium Substances 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 238000002823 phage display Methods 0.000 description 2
- 210000004180 plasmocyte Anatomy 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 238000002864 sequence alignment Methods 0.000 description 2
- 238000002741 site-directed mutagenesis Methods 0.000 description 2
- 230000009870 specific binding Effects 0.000 description 2
- 230000008685 targeting Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 230000005030 transcription termination Effects 0.000 description 2
- 230000009261 transgenic effect Effects 0.000 description 2
- 108091005703 transmembrane proteins Proteins 0.000 description 2
- 102000035160 transmembrane proteins Human genes 0.000 description 2
- 229960000575 trastuzumab Drugs 0.000 description 2
- 239000013603 viral vector Substances 0.000 description 2
- JTTIOYHBNXDJOD-UHFFFAOYSA-N 2,4,6-triaminopyrimidine Chemical compound NC1=CC(N)=NC(N)=N1 JTTIOYHBNXDJOD-UHFFFAOYSA-N 0.000 description 1
- UFBJCMHMOXMLKC-UHFFFAOYSA-N 2,4-dinitrophenol Chemical compound OC1=CC=C([N+]([O-])=O)C=C1[N+]([O-])=O UFBJCMHMOXMLKC-UHFFFAOYSA-N 0.000 description 1
- UAIUNKRWKOVEES-UHFFFAOYSA-N 3,3',5,5'-tetramethylbenzidine Chemical compound CC1=C(N)C(C)=CC(C=2C=C(C)C(N)=C(C)C=2)=C1 UAIUNKRWKOVEES-UHFFFAOYSA-N 0.000 description 1
- 206010069754 Acquired gene mutation Diseases 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241000282421 Canidae Species 0.000 description 1
- 241000282472 Canis lupus familiaris Species 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 1
- 241000700198 Cavia Species 0.000 description 1
- 241000282994 Cervidae Species 0.000 description 1
- 241000195628 Chlorophyta Species 0.000 description 1
- MFYSYFVPBJMHGN-UHFFFAOYSA-N Cortisone Natural products O=C1CCC2(C)C3C(=O)CC(C)(C(CC4)(O)C(=O)CO)C4C3CCC2=C1 MFYSYFVPBJMHGN-UHFFFAOYSA-N 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 241000938605 Crocodylia Species 0.000 description 1
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000283074 Equus asinus Species 0.000 description 1
- 241000283073 Equus caballus Species 0.000 description 1
- 244000024675 Eruca sativa Species 0.000 description 1
- 241000350139 Erythrophleum suaveolens Species 0.000 description 1
- 241000206602 Eukaryota Species 0.000 description 1
- 241000282324 Felis Species 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000282818 Giraffidae Species 0.000 description 1
- 102000001554 Hemoglobins Human genes 0.000 description 1
- 108010054147 Hemoglobins Proteins 0.000 description 1
- 101000984653 Homo sapiens Large subunit GTPase 1 homolog Proteins 0.000 description 1
- 101000724418 Homo sapiens Neutral amino acid transporter B(0) Proteins 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 101710111858 Immunoglobulin gamma-1 heavy chain Proteins 0.000 description 1
- 102100026215 Immunoglobulin gamma-1 heavy chain Human genes 0.000 description 1
- 101710189008 Immunoglobulin kappa light chain Proteins 0.000 description 1
- 102100029567 Immunoglobulin kappa light chain Human genes 0.000 description 1
- 102100027113 Large subunit GTPase 1 homolog Human genes 0.000 description 1
- 239000012097 Lipofectamine 2000 Substances 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 102100028267 Neutral amino acid transporter B(0) Human genes 0.000 description 1
- 108091034117 Oligonucleotide Proteins 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 241000282579 Pan Species 0.000 description 1
- 241000282320 Panthera leo Species 0.000 description 1
- 241000282376 Panthera tigris Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 239000004743 Polypropylene Substances 0.000 description 1
- 241000282405 Pongo abelii Species 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 108050002653 Retinoblastoma protein Proteins 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 108010008038 Synthetic Vaccines Proteins 0.000 description 1
- 102000006601 Thymidine Kinase Human genes 0.000 description 1
- 108020004440 Thymidine kinase Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 101150044453 Y gene Proteins 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- CUJRVFIICFDLGR-UHFFFAOYSA-N acetylacetonate Chemical compound CC(=O)[CH-]C(C)=O CUJRVFIICFDLGR-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 229940088710 antibiotic agent Drugs 0.000 description 1
- 102000025171 antigen binding proteins Human genes 0.000 description 1
- 108091000831 antigen binding proteins Proteins 0.000 description 1
- 208000016063 arterial thoracic outlet syndrome Diseases 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 229960000397 bevacizumab Drugs 0.000 description 1
- 230000006287 biotinylation Effects 0.000 description 1
- 238000007413 biotinylation Methods 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000011712 cell development Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 238000013373 clone screening Methods 0.000 description 1
- 238000012761 co-transfection Methods 0.000 description 1
- 230000003918 constitutive secretory pathway Effects 0.000 description 1
- 239000013256 coordination polymer Substances 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000013024 dilution buffer Substances 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 238000010494 dissociation reaction Methods 0.000 description 1
- 230000005593 dissociations Effects 0.000 description 1
- 210000001163 endosome Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000013613 expression plasmid Substances 0.000 description 1
- 238000005206 flow analysis Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000007446 host cell death Effects 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 210000003297 immature b lymphocyte Anatomy 0.000 description 1
- 238000001114 immunoprecipitation Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 239000012678 infectious agent Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 229920000609 methyl cellulose Polymers 0.000 description 1
- 239000001923 methylcellulose Substances 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 241000264288 mixed libraries Species 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 108091005706 peripheral membrane proteins Proteins 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 230000007505 plaque formation Effects 0.000 description 1
- 230000008488 polyadenylation Effects 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- -1 polypropylene Polymers 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 230000004481 post-translational protein modification Effects 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 210000001948 pro-b lymphocyte Anatomy 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- XJMOSONTPMZWPB-UHFFFAOYSA-M propidium iodide Chemical compound [I-].[I-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CCC[N+](C)(CC)CC)=C1C1=CC=CC=C1 XJMOSONTPMZWPB-UHFFFAOYSA-M 0.000 description 1
- 238000002818 protein evolution Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 229940124551 recombinant vaccine Drugs 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 230000001177 retroviral effect Effects 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- LPXPTNMVRIOKMN-UHFFFAOYSA-M sodium nitrite Substances [Na+].[O-]N=O LPXPTNMVRIOKMN-UHFFFAOYSA-M 0.000 description 1
- 230000037439 somatic mutation Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010257 thawing Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
- 229940124954 vaccinia virus vaccine Drugs 0.000 description 1
- 239000011534 wash buffer Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/005—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/52—Constant or Fc region; Isotype
- C07K2317/522—CH1 domain
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/24011—Poxviridae
- C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
- C12N2710/24122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/24011—Poxviridae
- C12N2710/24111—Orthopoxvirus, e.g. vaccinia virus, variola
- C12N2710/24141—Use of virus, viral particle or viral elements as a vector
- C12N2710/24143—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B30/00—Methods of screening libraries
- C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
- C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Virology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Oncology (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261639046P | 2012-04-26 | 2012-04-26 | |
| US201261732776P | 2012-12-03 | 2012-12-03 | |
| US13/844,388 US9708601B2 (en) | 2012-04-26 | 2013-03-15 | Fusion proteins to facilitate selection of cells infected with specific immunoglobulin gene recombinant vaccinia virus |
| PCT/US2013/038497 WO2013163602A1 (en) | 2012-04-26 | 2013-04-26 | Fusion proteins to facilitate selection of cells infected with specific immunoglobulin gene recombinant vaccinia virus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EA201491965A1 EA201491965A1 (ru) | 2015-03-31 |
| EA028164B1 true EA028164B1 (ru) | 2017-10-31 |
Family
ID=49477810
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EA201491965A EA028164B1 (ru) | 2012-04-26 | 2013-04-26 | Слитые белки для облегчения отбора клеток, инфицированных рекомбинантным вирусом осповакцины с геном специфического иммуноглобулина |
Country Status (16)
| Country | Link |
|---|---|
| US (3) | US9708601B2 (enExample) |
| EP (1) | EP2841606B1 (enExample) |
| JP (2) | JP6498599B2 (enExample) |
| KR (1) | KR102108589B1 (enExample) |
| CN (1) | CN104520444B (enExample) |
| AU (1) | AU2013251371B2 (enExample) |
| CA (1) | CA2871597C (enExample) |
| DK (1) | DK2841606T3 (enExample) |
| EA (1) | EA028164B1 (enExample) |
| ES (1) | ES2725673T3 (enExample) |
| IL (1) | IL235323B (enExample) |
| NZ (1) | NZ630854A (enExample) |
| PL (1) | PL2841606T3 (enExample) |
| PT (1) | PT2841606T (enExample) |
| SG (1) | SG11201406974YA (enExample) |
| WO (1) | WO2013163602A1 (enExample) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| NO2427212T3 (enExample) | 2009-05-08 | 2018-01-20 | ||
| CN104168956A (zh) | 2011-10-11 | 2014-11-26 | 瓦西尼斯公司 | 臂板蛋白-4d结合分子用于调节血脑屏障渗透性的用途 |
| US9708601B2 (en) | 2012-04-26 | 2017-07-18 | Vaccinex, Inc. | Fusion proteins to facilitate selection of cells infected with specific immunoglobulin gene recombinant vaccinia virus |
| US10494440B2 (en) | 2012-05-11 | 2019-12-03 | Vaccinex, Inc. | Use of semaphorin-4D binding molecules to promote neurogenesis following stroke |
| BR112015032690B1 (pt) | 2013-06-25 | 2020-03-10 | Vaccinex, Inc. | Uso de moléculas inibidoras de semaforina-4d em combinação com uma terapia imunomoduladora para inibir o crescimento tumoral e metástase |
| NZ630881A (en) | 2013-10-10 | 2016-03-31 | Vaccinex Inc | Use of semaphorin-4d binding molecules for treatment of atherosclerosis |
| NZ630892A (en) | 2013-10-21 | 2016-03-31 | Vaccinex Inc | Use of semaphorin-4d binding molecules for treating neurodegenerative disorders |
| CA2939572A1 (en) * | 2014-02-19 | 2015-08-27 | Jody Berry | Marburg monoclonal antibodies |
| KR20170019080A (ko) | 2015-08-11 | 2017-02-21 | 주식회사 엘티전자 | 플렉시블 사이니지 장치 |
| EP3402520A4 (en) * | 2016-01-14 | 2019-01-02 | BPS Bioscience, Inc. | Anti-pd-1 antibodies and uses thereof |
| BR112018071686A2 (pt) * | 2016-04-22 | 2019-02-19 | Vaccinex, Inc. | exibição de proteínas integrais de membrana em vírions extracelulares envelopados de poxvírus |
| WO2018026715A1 (en) | 2016-08-02 | 2018-02-08 | Vaccinex, Inc. | Improved methods for producing polynucleotide libraries in vaccinia virus/eukaryotic cells |
| CN110636858B (zh) | 2017-05-05 | 2024-03-19 | 瓦西尼斯公司 | 人抗脑信号蛋白4d抗体 |
| AU2021267163B2 (en) | 2020-05-06 | 2024-05-23 | Vaccinex, Inc. | Integral membrane protein display on poxvirus extracellular enveloped virions |
| IL317110A (en) | 2022-06-10 | 2025-01-01 | Vaccinex Inc | Methods for selecting antibodies specific for complex membrane antigens |
| WO2025184411A1 (en) | 2024-02-27 | 2025-09-04 | Calidi Biotherapeutics (Nevada), Inc. | Serum-resistant eev viruses and uses thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050266425A1 (en) * | 2003-12-31 | 2005-12-01 | Vaccinex, Inc. | Methods for producing and identifying multispecific antibodies |
| US20090304627A1 (en) * | 2008-05-28 | 2009-12-10 | Vgx Pharmaceuticals, Inc. | Smallpox dna vaccine and the antigens therein that elicit an immune response |
| US20100081575A1 (en) * | 2008-09-22 | 2010-04-01 | Robert Anthony Williamson | Methods for creating diversity in libraries and libraries, display vectors and methods, and displayed molecules |
Family Cites Families (48)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5445953A (en) | 1991-08-26 | 1995-08-29 | Immuno Aktiengesellschaft | Direct molecular cloning of a modified poxvirus genome |
| EP0758397B1 (en) | 1994-04-29 | 2005-06-22 | Baxter Healthcare S.A. | Recombinant poxviruses with foreign polynucleotides in essential regions |
| US6576754B2 (en) | 1995-11-09 | 2003-06-10 | Dana-Farber Cancer Institute | CD100 antigen and uses therefor |
| US6872518B2 (en) | 1997-09-22 | 2005-03-29 | University Of Rochester | Methods for selecting polynucleotides encoding T cell epitopes |
| AU776865B2 (en) | 1998-11-10 | 2004-09-23 | University Of Rochester | T cells specific for target antigens and methods and vaccines based thereon |
| US7067251B2 (en) | 2000-03-28 | 2006-06-27 | University Of Rochester | Methods of directly selecting cells expressing inserts of interest |
| CA2341356C (en) * | 2000-04-14 | 2011-10-11 | Transgene S.A. | Poxvirus with targeted infection specificity |
| AU2001297872B2 (en) * | 2000-11-17 | 2006-11-09 | University Of Rochester | In vitro methods of producing and identifying immunoglobulin molecules in eukaryotic cells |
| AU2002255495A1 (en) | 2001-02-02 | 2002-08-19 | University Of Rochester | Methods of identifying regulator molecules |
| US9809654B2 (en) | 2002-09-27 | 2017-11-07 | Vaccinex, Inc. | Targeted CD1d molecules |
| EP1413316A1 (en) | 2002-09-27 | 2004-04-28 | Bruno Robert | Bifunctional conjugates or fusion proteins |
| EP1442749A1 (en) | 2003-01-31 | 2004-08-04 | Institut National De La Sante Et De La Recherche Medicale (Inserm) | Use of anti-CD100 antibodies for the treatment and the diagnosis of inflammatory disorder affecting the central nervous system |
| JP5042631B2 (ja) | 2003-12-04 | 2012-10-03 | バクシネックス インコーポレーティッド | アポトーシス腫瘍細胞上に露出した細胞内抗原をターゲッティングすることによって腫瘍細胞を死滅させる方法 |
| AU2005280163B2 (en) | 2004-08-27 | 2011-11-24 | Albert Einstein College Of Medicine, Inc. | Ceramide derivatives as modulators of immunity and autoimmunity |
| US8022043B2 (en) | 2004-08-27 | 2011-09-20 | Albert Einstein College Of Medicine Of Yeshiva University | Ceramide derivatives as modulators of immunity and autoimmunity |
| US7858599B2 (en) | 2005-12-30 | 2010-12-28 | Hander Robert W | Enhancement of urogenital function |
| US9382327B2 (en) | 2006-10-10 | 2016-07-05 | Vaccinex, Inc. | Anti-CD20 antibodies and methods of use |
| EP2069404B1 (en) | 2007-02-14 | 2011-01-05 | Vaccinex, Inc. | Humanized anti-cd100 antibodies |
| EP2112930B1 (en) | 2007-02-21 | 2017-01-11 | Vaccinex, Inc. | Modulation of nkt cell activity with antigen-loaded cdid molecules |
| EP2237792B1 (en) | 2007-12-26 | 2017-05-24 | Vaccinex, Inc. | Anti-c35 antibody combination therapies and methods |
| KR20110137290A (ko) | 2009-01-08 | 2011-12-22 | 알버트 아인슈타인 컬리지 오브 메디신 오브 예쉬바 유니버시티 | 세포벽 결합 세라미드 유사 당지질을 갖는 박테리아 백신 및 이의 용도 |
| NO2427212T3 (enExample) | 2009-05-08 | 2018-01-20 | ||
| RS66008B1 (sr) * | 2010-03-10 | 2024-10-31 | Genmab As | Monoklonska antitela protiv c-met |
| TW201134488A (en) * | 2010-03-11 | 2011-10-16 | Ucb Pharma Sa | PD-1 antibodies |
| AU2011248083B2 (en) * | 2010-05-04 | 2015-09-10 | Five Prime Therapeutics, Inc. | Antibodies that bind CSF1R |
| SG185487A1 (en) * | 2010-05-11 | 2012-12-28 | Aveo Pharmaceuticals Inc | Anti-fgfr2 antibodies |
| US8834883B2 (en) | 2010-06-14 | 2014-09-16 | Vaccinex, Inc. | Anti-VEGF antibodies and uses thereof |
| EP2404936A1 (en) * | 2010-07-06 | 2012-01-11 | Ganymed Pharmaceuticals AG | Cancer therapy using CLDN6 target-directed antibodies in vivo |
| CA2804399A1 (en) * | 2010-07-06 | 2012-01-12 | Aveo Pharmaceuticals, Inc. | Anti-ron antibodies |
| US20130164325A1 (en) | 2010-07-07 | 2013-06-27 | Albert Einstein College Of Medicine Of Yeshiva University | Ceramide-like glycolipid-associated bacterial vaccines and uses thereof |
| AU2011295902B2 (en) | 2010-09-02 | 2014-12-04 | Vaccinex, Inc. | Anti-CXCL13 antibodies and methods of using the same |
| US9447191B2 (en) | 2011-05-13 | 2016-09-20 | National University Corporation Tokyo Medical And Dental University | Osteogenesis promoter |
| CN104168956A (zh) | 2011-10-11 | 2014-11-26 | 瓦西尼斯公司 | 臂板蛋白-4d结合分子用于调节血脑屏障渗透性的用途 |
| US8790652B2 (en) | 2011-12-06 | 2014-07-29 | Vaccinex, Inc. | Use of the combination of semaphorin-4D inhibitory molecules and VEGF inhibitory molecules to inhibit angiogenesis |
| IN2014DN08199A (enExample) | 2012-03-02 | 2015-05-01 | Vaccinex Inc | |
| US9090709B2 (en) | 2012-03-28 | 2015-07-28 | Vaccinex, Inc. | Anti-SEMA4D antibodies and epitopes |
| US9708601B2 (en) | 2012-04-26 | 2017-07-18 | Vaccinex, Inc. | Fusion proteins to facilitate selection of cells infected with specific immunoglobulin gene recombinant vaccinia virus |
| US10494440B2 (en) | 2012-05-11 | 2019-12-03 | Vaccinex, Inc. | Use of semaphorin-4D binding molecules to promote neurogenesis following stroke |
| EP2951204B1 (en) | 2013-01-31 | 2019-05-22 | Vaccinex, Inc. | Methods for increasing immunoglobulin a levels |
| US9371352B2 (en) | 2013-02-08 | 2016-06-21 | Vaccinex, Inc. | Modified glycolipids and methods of making and using the same |
| BR112015032690B1 (pt) | 2013-06-25 | 2020-03-10 | Vaccinex, Inc. | Uso de moléculas inibidoras de semaforina-4d em combinação com uma terapia imunomoduladora para inibir o crescimento tumoral e metástase |
| NZ630881A (en) | 2013-10-10 | 2016-03-31 | Vaccinex Inc | Use of semaphorin-4d binding molecules for treatment of atherosclerosis |
| NZ630892A (en) | 2013-10-21 | 2016-03-31 | Vaccinex Inc | Use of semaphorin-4d binding molecules for treating neurodegenerative disorders |
| BR112018071686A2 (pt) | 2016-04-22 | 2019-02-19 | Vaccinex, Inc. | exibição de proteínas integrais de membrana em vírions extracelulares envelopados de poxvírus |
| WO2018026715A1 (en) | 2016-08-02 | 2018-02-08 | Vaccinex, Inc. | Improved methods for producing polynucleotide libraries in vaccinia virus/eukaryotic cells |
| WO2018156509A1 (en) | 2017-02-22 | 2018-08-30 | Vaccinex, Inc. | Early detection of glial cell activation in neurodegenerative or neuroinflammatory diseases |
| DK3600419T5 (en) | 2017-03-20 | 2024-05-27 | Vaccinex Inc | Treatment of cancer with a semaphorin-4d antibody in combination with an epigenetic modulating agent |
| CN110636858B (zh) | 2017-05-05 | 2024-03-19 | 瓦西尼斯公司 | 人抗脑信号蛋白4d抗体 |
-
2013
- 2013-03-15 US US13/844,388 patent/US9708601B2/en active Active
- 2013-04-26 DK DK13780660.0T patent/DK2841606T3/da active
- 2013-04-26 EA EA201491965A patent/EA028164B1/ru not_active IP Right Cessation
- 2013-04-26 KR KR1020147033046A patent/KR102108589B1/ko active Active
- 2013-04-26 WO PCT/US2013/038497 patent/WO2013163602A1/en not_active Ceased
- 2013-04-26 EP EP13780660.0A patent/EP2841606B1/en active Active
- 2013-04-26 ES ES13780660T patent/ES2725673T3/es active Active
- 2013-04-26 AU AU2013251371A patent/AU2013251371B2/en active Active
- 2013-04-26 CA CA2871597A patent/CA2871597C/en active Active
- 2013-04-26 PT PT13780660T patent/PT2841606T/pt unknown
- 2013-04-26 CN CN201380033060.7A patent/CN104520444B/zh active Active
- 2013-04-26 NZ NZ630854A patent/NZ630854A/en unknown
- 2013-04-26 SG SG11201406974YA patent/SG11201406974YA/en unknown
- 2013-04-26 JP JP2015509201A patent/JP6498599B2/ja active Active
- 2013-04-26 PL PL13780660T patent/PL2841606T3/pl unknown
-
2014
- 2014-10-23 IL IL235323A patent/IL235323B/en active IP Right Grant
-
2015
- 2015-12-21 US US14/977,067 patent/US9701958B2/en active Active
-
2017
- 2017-06-12 US US15/619,790 patent/US10662422B2/en active Active
-
2018
- 2018-11-01 JP JP2018206485A patent/JP2019055956A/ja not_active Withdrawn
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050266425A1 (en) * | 2003-12-31 | 2005-12-01 | Vaccinex, Inc. | Methods for producing and identifying multispecific antibodies |
| US20090304627A1 (en) * | 2008-05-28 | 2009-12-10 | Vgx Pharmaceuticals, Inc. | Smallpox dna vaccine and the antigens therein that elicit an immune response |
| US20100081575A1 (en) * | 2008-09-22 | 2010-04-01 | Robert Anthony Williamson | Methods for creating diversity in libraries and libraries, display vectors and methods, and displayed molecules |
Non-Patent Citations (2)
| Title |
|---|
| GALMICHE et al. "Expression of a functional single chain antibody on the surface of extracellular enveloped vaccinia virus as a step towards selective tumour cell targeting," Journal of General Virology, 01 November 1997 (01.11.1997), vol. 78, pgs. 3019-3027. entire document * |
| SMITH et al. "Nucleotide sequence of 42 kbp of vaccinia virus strain WR from near the right inverted terminal repeat," Journal of General Virology, 01 June 1991 (01.06.1991), vol. 72, pgs. 1349-1376, entire document * |
Also Published As
| Publication number | Publication date |
|---|---|
| CA2871597A1 (en) | 2013-10-31 |
| AU2013251371B2 (en) | 2018-01-04 |
| JP2015514811A (ja) | 2015-05-21 |
| CA2871597C (en) | 2021-04-20 |
| PT2841606T (pt) | 2019-05-17 |
| US20170306318A1 (en) | 2017-10-26 |
| US9708601B2 (en) | 2017-07-18 |
| EP2841606A4 (en) | 2015-12-30 |
| SG11201406974YA (en) | 2014-11-27 |
| US20130288927A1 (en) | 2013-10-31 |
| US9701958B2 (en) | 2017-07-11 |
| EA201491965A1 (ru) | 2015-03-31 |
| JP2019055956A (ja) | 2019-04-11 |
| NZ630854A (en) | 2017-03-31 |
| PL2841606T3 (pl) | 2019-07-31 |
| KR20150009560A (ko) | 2015-01-26 |
| IL235323B (en) | 2020-01-30 |
| CN104520444B (zh) | 2017-12-12 |
| ES2725673T3 (es) | 2019-09-26 |
| IL235323A0 (en) | 2014-12-31 |
| KR102108589B1 (ko) | 2020-05-07 |
| CN104520444A (zh) | 2015-04-15 |
| DK2841606T3 (da) | 2019-05-06 |
| EP2841606A1 (en) | 2015-03-04 |
| JP6498599B2 (ja) | 2019-04-10 |
| AU2013251371A1 (en) | 2014-11-13 |
| US20160152971A1 (en) | 2016-06-02 |
| WO2013163602A1 (en) | 2013-10-31 |
| US10662422B2 (en) | 2020-05-26 |
| EP2841606B1 (en) | 2019-01-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EA028164B1 (ru) | Слитые белки для облегчения отбора клеток, инфицированных рекомбинантным вирусом осповакцины с геном специфического иммуноглобулина | |
| US12129291B2 (en) | Anti-GPC3 antibody | |
| JP6043431B2 (ja) | 特異的な結合タンパク質または機能性タンパク質の遺伝子転位を利用した同定 | |
| Karachaliou et al. | IgY technology: Methods for developing and evaluating avian immunoglobulins for the in vitro detection of biomolecules | |
| RU2625033C2 (ru) | Система индикации на основе полноразмерного антитела для эукариотических клеток и ее применение | |
| RU2759846C2 (ru) | Дисплей интегрального мембранного белка на внеклеточных оболочечных вирионах поксвируса | |
| JP7141154B2 (ja) | 抗体ライブラリーの構築方法およびその使用 | |
| CN107353340B (zh) | 抗h7n9全人源单克隆抗体2l11及其制法与应用 | |
| TW200907059A (en) | Recombinant expression vector elements (rEVEs) for enhancing expression of recombinant proteins in host cells | |
| JP2003512860A (ja) | 組換え融合分子 | |
| US20230060376A1 (en) | B cell receptor modification in b cells | |
| Bergman et al. | Preferential targeting of vesicular stomatitis virus to breast cancer cells | |
| Yan et al. | Preparation of a new monoclonal antibody against subgroup A of avian leukosis virus and identifying its antigenic epitope | |
| JP2022521147A (ja) | ｍＲＮＡディスプレイ抗体ライブラリー及び方法 | |
| EP2311493A1 (en) | Cytotoxic composition | |
| RU2829359C1 (ru) | Универсальный интеграционный вектор SB7G_HIGH и рекомбинантная плазмида SB7G_HIGH_DEV_57, обеспечивающая синтез и секрецию рекомбинантного человеческого антитела к рецептор-связывающему домену (RBD) коронавируса SARS-CoV-2 в клетках млекопитающих и полученная с использованием универсального вектора SB7G_HIGH, и рекомбинантное моноклональное антитело DEV_K57, обладающее вируснейтрализующей активностью в отношении SARS-CoV-2 | |
| CN104507970B (zh) | 通过抑制p68与钙调蛋白相互作用来预防癌症转移及抑制炎症的方法 | |
| KR102266878B1 (ko) | 감자 잎말림 바이러스 특이적 Scfv 항체 및 이의 용도 | |
| JP2011103876A (ja) | キメラ抗体の一段階作製方法 | |
| JP2015163047A (ja) | タンパク質の迅速改良法 | |
| EA043515B1 (ru) | Анти-gpc3-антитело | |
| Deen | Isolation of Human Endoplasmic Reticulum Antibodies Using Phage Display Technology |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): AM AZ BY KZ KG TJ TM |