EA020145B9 - Гидролазы, кодирующие их нуклеиновые кислоты и способы их получения и применения - Google Patents
Гидролазы, кодирующие их нуклеиновые кислоты и способы их получения и примененияInfo
- Publication number
- EA020145B9 EA020145B9 EA201170388A EA201170388A EA020145B9 EA 020145 B9 EA020145 B9 EA 020145B9 EA 201170388 A EA201170388 A EA 201170388A EA 201170388 A EA201170388 A EA 201170388A EA 020145 B9 EA020145 B9 EA 020145B9
- Authority
- EA
- Eurasian Patent Office
- Prior art keywords
- methods
- hydrolases
- making
- nucleic acids
- acids encoding
- Prior art date
Links
- 108090000604 Hydrolases Proteins 0.000 title abstract 3
- 102000004157 Hydrolases Human genes 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
- -1 saturases Proteins 0.000 abstract 3
- 108090001060 Lipase Proteins 0.000 abstract 2
- 102000004882 Lipase Human genes 0.000 abstract 2
- 239000004367 Lipase Substances 0.000 abstract 2
- 235000019421 lipase Nutrition 0.000 abstract 2
- 108010047498 long-chain carboxylesterase Proteins 0.000 abstract 2
- 102000040430 polynucleotide Human genes 0.000 abstract 2
- 108091033319 polynucleotide Proteins 0.000 abstract 2
- 239000002157 polynucleotide Substances 0.000 abstract 2
- 229920001184 polypeptide Polymers 0.000 abstract 2
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 2
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 2
- 235000010692 trans-unsaturated fatty acids Nutrition 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 239000010775 animal oil Substances 0.000 abstract 1
- 239000000828 canola oil Substances 0.000 abstract 1
- 239000003921 oil Substances 0.000 abstract 1
- 235000019198 oils Nutrition 0.000 abstract 1
- 235000015112 vegetable and seed oil Nutrition 0.000 abstract 1
- 239000008158 vegetable oil Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/18—Carboxylic ester hydrolases (3.1.1)
- C12N9/20—Triglyceride splitting, e.g. by means of lipase
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/66—Enzymes
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11D—DETERGENT COMPOSITIONS; USE OF SINGLE SUBSTANCES AS DETERGENTS; SOAP OR SOAP-MAKING; RESIN SOAPS; RECOVERY OF GLYCEROL
- C11D3/00—Other compounding ingredients of detergent compositions covered in group C11D1/00
- C11D3/16—Organic compounds
- C11D3/38—Products with no well-defined composition, e.g. natural products
- C11D3/386—Preparations containing enzymes, e.g. protease or amylase
- C11D3/38681—Chemically modified or immobilised enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/10—General cosmetic use
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
- C12Y301/01021—Retinyl-palmitate esterase (3.1.1.21)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Medicinal Chemistry (AREA)
- Public Health (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Dermatology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Cosmetics (AREA)
- Fats And Perfumes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
В изобретении предлагаются гидролазы, включающие липазы, сатуразы, пальмитазы и/или стеаратазы и кодирующие их полинуклеотиды, а также способы получения и применения этих полинуклеотидов и полипептидов. Дополнительно предлагаются полипептиды, например ферменты, обладающие гидролазной активностью, например липазы, сатуразы, пальмитазы и/или стеаратазы, и способы получения масел низкой насыщенности или имеющих низкое содержание жирных транс-кислот, таких как животные или растительные масла низкой насыщенности, или имеющие низкое содержание жирных транс-кислот, например масло сои или канолы.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US12/202,119 US8198062B2 (en) | 2008-08-29 | 2008-08-29 | Hydrolases, nucleic acids encoding them and methods for making and using them |
PCT/US2009/055412 WO2010025395A2 (en) | 2008-08-29 | 2009-08-28 | Hydrolases, nucleic acids encoding them and methods for making and using them |
Publications (3)
Publication Number | Publication Date |
---|---|
EA201170388A1 EA201170388A1 (ru) | 2011-10-31 |
EA020145B1 EA020145B1 (ru) | 2014-09-30 |
EA020145B9 true EA020145B9 (ru) | 2014-11-28 |
Family
ID=41722322
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EA201170388A EA020145B9 (ru) | 2008-08-29 | 2009-08-28 | Гидролазы, кодирующие их нуклеиновые кислоты и способы их получения и применения |
Country Status (11)
Country | Link |
---|---|
US (4) | US8198062B2 (ru) |
EP (1) | EP2328907B1 (ru) |
KR (1) | KR20110050703A (ru) |
CN (1) | CN102197043B (ru) |
AR (1) | AR073942A1 (ru) |
BR (1) | BRPI0918265A8 (ru) |
CA (2) | CA2735230C (ru) |
EA (1) | EA020145B9 (ru) |
ES (1) | ES2714673T3 (ru) |
MX (1) | MX2011002297A (ru) |
WO (1) | WO2010025395A2 (ru) |
Families Citing this family (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP2620446A1 (en) | 2012-01-27 | 2013-07-31 | Laboratorios Del Dr. Esteve, S.A. | Immunogens for HIV vaccination |
CN103421788B (zh) * | 2013-04-27 | 2015-06-10 | 华中农业大学 | 水稻逆境应答型启动子OsSN1P的分离和利用 |
EP3243897B2 (en) | 2016-05-09 | 2022-02-16 | The Procter & Gamble Company | Detergent composition comprising an fatty acid-transforming enzyme |
ES2835648T3 (es) | 2016-05-09 | 2021-06-22 | Procter & Gamble | Composición detergente que comprende una descarboxilasa de ácidos grasos |
CN109072137B (zh) * | 2016-05-09 | 2021-06-29 | 宝洁公司 | 包含油酸转化酶的洗涤剂组合物 |
EP3694339A4 (en) * | 2017-10-12 | 2021-07-21 | Syngenta Participations AG | IMPROVED FEEDING COMPOSITIONS AND METHODS OF USE |
CN108559735B (zh) * | 2018-05-10 | 2020-07-07 | 江南大学 | 一种亮氨酸脱氢酶突变体的构建及其应用 |
US11666651B2 (en) | 2019-11-14 | 2023-06-06 | Aelix Therapeutics, S.L. | Prime/boost immunization regimen against HIV-1 utilizing a multiepitope T cell immunogen comprising Gag, Pol, Vif, and Nef epitopes |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20070202566A1 (en) * | 2003-03-07 | 2007-08-30 | Bornscheuer Uwe T | Hydrolases, Nucleic Acids Encoding Them And Methods For Making And Using Them |
Family Cites Families (160)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3949105A (en) * | 1974-03-25 | 1976-04-06 | Lever Brothers Company | Margarine fat |
US4261868A (en) * | 1979-08-08 | 1981-04-14 | Lever Brothers Company | Stabilized enzymatic liquid detergent composition containing a polyalkanolamine and a boron compound |
US4458066A (en) | 1980-02-29 | 1984-07-03 | University Patents, Inc. | Process for preparing polynucleotides |
IE52535B1 (en) * | 1981-02-16 | 1987-12-09 | Ici Plc | Continuous release pharmaceutical compositions |
US4404128A (en) * | 1981-05-29 | 1983-09-13 | The Procter & Gamble Company | Enzyme detergent composition |
IE54838B1 (en) | 1982-04-30 | 1990-02-28 | Unilever Plc | Improvements in and relating to interesterification of triglycerides of fatty acids |
DK402583D0 (da) * | 1983-09-05 | 1983-09-05 | Novo Industri As | Fremgangsmade til fremstilling af et immobiliseret lipasepraeparat og anvendelse deraf |
US4707364A (en) * | 1984-01-27 | 1987-11-17 | Miles Laboratories, Inc. | Composition for accelerating cheese aging |
US5204015A (en) * | 1984-05-29 | 1993-04-20 | Genencor International, Inc. | Subtilisin mutants |
US5087571A (en) | 1984-06-22 | 1992-02-11 | President And Fellows Of Harvard College | Method for providing a cell culture from a transgenic non-human mammal |
US5691181A (en) * | 1984-08-21 | 1997-11-25 | Celltech Limited | DNA encoding lipase from human gastric mucosal tissue |
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
US4683195A (en) | 1986-01-30 | 1987-07-28 | Cetus Corporation | Process for amplifying, detecting, and/or-cloning nucleic acid sequences |
WO1986005803A1 (fr) | 1985-03-30 | 1986-10-09 | Marc Ballivet | Procede d'obtention d'adn, arn, peptides, polypeptides ou proteines, par une technique de recombinaison d'adn |
US4752483A (en) * | 1986-05-30 | 1988-06-21 | Campbell Soup Company | Method for producing a highly flavored cheese ingredient |
US4962028A (en) | 1986-07-09 | 1990-10-09 | Dna Plant Technology Corporation | Plant promotors |
WO1988001213A1 (en) * | 1986-08-18 | 1988-02-25 | Clinical Technologies Associates, Inc. | Delivery systems for pharmacological agents |
US4946778A (en) | 1987-09-21 | 1990-08-07 | Genex Corporation | Single polypeptide chain binding molecules |
US4987071A (en) | 1986-12-03 | 1991-01-22 | University Patents, Inc. | RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods |
US5015580A (en) | 1987-07-29 | 1991-05-14 | Agracetus | Particle-mediated transformation of soybean plants and lines |
US5573933A (en) | 1987-04-14 | 1996-11-12 | Luminis Pty, Ltd. | Transgenic pigs |
US4897268A (en) * | 1987-08-03 | 1990-01-30 | Southern Research Institute | Drug delivery system and method of making the same |
US4944944A (en) * | 1987-11-19 | 1990-07-31 | Oklahoma Medical Research Foundation | Dietary compositions and methods using bile salt-activated lipase |
US6054270A (en) | 1988-05-03 | 2000-04-25 | Oxford Gene Technology Limited | Analying polynucleotide sequences |
US5700637A (en) | 1988-05-03 | 1997-12-23 | Isis Innovation Limited | Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays |
US5000975A (en) * | 1988-12-29 | 1991-03-19 | American Home Products Corporation | Randomized palm oil fat composition for infant formulas |
US5217879A (en) | 1989-01-12 | 1993-06-08 | Washington University | Infectious Sindbis virus vectors |
US5069810A (en) * | 1989-03-16 | 1991-12-03 | Olin Corporation | Cleaning composition comprising microbial lipase SD2 and sodium dodecylbenzene sulfonate |
US5800992A (en) | 1989-06-07 | 1998-09-01 | Fodor; Stephen P.A. | Method of detecting nucleic acids |
US5527681A (en) | 1989-06-07 | 1996-06-18 | Affymax Technologies N.V. | Immobilized molecular synthesis of systematically substituted compounds |
US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
US5744101A (en) | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
US5288619A (en) * | 1989-12-18 | 1994-02-22 | Kraft General Foods, Inc. | Enzymatic method for preparing transesterified oils |
US5589583A (en) | 1990-01-11 | 1996-12-31 | Monsanto Company | Plant promoter |
AU7791991A (en) | 1990-04-24 | 1991-11-11 | Stratagene | Methods for phenotype creation from multiple gene populations |
ES2217250T3 (es) | 1990-06-15 | 2004-11-01 | Scios Inc. | Mamifero transgenico, no humano que muestra la patologia de formacion amiloides de la enfermedad de alzheimer. |
CA2096843C (en) | 1990-11-23 | 2007-08-07 | Kathleen D'halluin | Process for transforming monocotyledonous plants |
ATE177782T1 (de) | 1990-12-20 | 1999-04-15 | Ixsys Inc | Optimierung von bindenden proteinen |
US6110700A (en) | 1991-03-11 | 2000-08-29 | The General Hospital Corporation | PRAD1 cyclin and its cDNA |
DE4115938A1 (de) * | 1991-05-16 | 1992-11-19 | Metallgesellschaft Ag | Enzymatisches verfahren zur verminderung des gehaltes an phosphorhaltigen bestandteilen in pflanzlichen und tierischen oelen |
US5922854A (en) | 1991-06-14 | 1999-07-13 | Kumar; Ramesh | Purifying Human Hemogloblin from transgenic pig red cells and plasmids containing pig globin nucleic acids |
CA2075135A1 (en) | 1991-08-02 | 1993-02-03 | David E. Ellis | Particle-mediated transformation of gymnosperms |
US5632957A (en) | 1993-11-01 | 1997-05-27 | Nanogen | Molecular biological diagnostic systems including electrodes |
JPH05236997A (ja) | 1992-02-28 | 1993-09-17 | Hitachi Ltd | ポリヌクレオチド捕捉用チップ |
US5902617A (en) * | 1992-05-19 | 1999-05-11 | Pabst; Patrea L. | Enzyme supplemented baby formula |
US5445955A (en) * | 1992-05-25 | 1995-08-29 | The Nisshin Oil Mills, Ltd. | Immobilization of lipase on a polymer carrier containing epoxy and tertiary amino groups |
EP0571982A1 (en) * | 1992-05-27 | 1993-12-01 | Showa Denko Kabushiki Kaisha | Alkaline lipase, method for producing the same, microorganism producing the same and detergent composition containing alkaline lipase |
AU5676394A (en) | 1992-11-20 | 1994-06-22 | Agracetus, Inc. | Transgenic cotton plants producing heterologous bioplastic |
DK0698090T3 (da) * | 1993-05-20 | 1998-08-10 | Loders Croklaan Bv | Fremgangsmåde til fremstilling af immobiliserede lipaser |
JP2859520B2 (ja) * | 1993-08-30 | 1999-02-17 | ノボ ノルディスク アクティーゼルスカブ | リパーゼ及びそれを生産する微生物及びリパーゼ製造方法及びリパーゼ含有洗剤組成物 |
AU7925094A (en) | 1993-09-30 | 1995-04-18 | Agracetus, Inc. | Transgenic cotton plants producing heterologous peroxidase |
US6045996A (en) | 1993-10-26 | 2000-04-04 | Affymetrix, Inc. | Hybridization assays on oligonucleotide arrays |
US6468742B2 (en) | 1993-11-01 | 2002-10-22 | Nanogen, Inc. | Methods for determination of single nucleic acid polymorphisms using bioelectronic microchip |
US5965452A (en) | 1996-07-09 | 1999-10-12 | Nanogen, Inc. | Multiplexed active biologic array |
US5834252A (en) | 1995-04-18 | 1998-11-10 | Glaxo Group Limited | End-complementary polymerase reaction |
US6335160B1 (en) | 1995-02-17 | 2002-01-01 | Maxygen, Inc. | Methods and compositions for polypeptide engineering |
US5837458A (en) | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
US6117679A (en) | 1994-02-17 | 2000-09-12 | Maxygen, Inc. | Methods for generating polynucleotides having desired characteristics by iterative selection and recombination |
US5639940A (en) | 1994-03-03 | 1997-06-17 | Pharmaceutical Proteins Ltd. | Production of fibrinogen in transgenic animals |
AU692841B2 (en) | 1994-03-09 | 1998-06-18 | Abbott Laboratories | Transgenic animals producing oligosaccharides and glycoconjugates |
CA2184687A1 (en) | 1994-03-09 | 1995-09-14 | Pedro Antonio Prieto | Humanized milk |
CA2186592C (en) | 1994-03-29 | 2008-02-19 | Helle Outtrup | Alkaline bacillus amylase |
US5750870A (en) | 1994-06-17 | 1998-05-12 | Agritope, Inc. | Plant genetic transformation methods and transgenic plants |
US5807522A (en) | 1994-06-17 | 1998-09-15 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for fabricating microarrays of biological samples |
US5959171A (en) | 1994-08-17 | 1999-09-28 | Pharming B.V. | Method for the production of biologically active polypeptides in a mammal's |
WO1996006927A1 (en) | 1994-09-01 | 1996-03-07 | Merck & Co., Inc. | Transgenic animal expressing a familial form of human amyloid precursor protein |
US5795737A (en) | 1994-09-19 | 1998-08-18 | The General Hospital Corporation | High level expression of proteins |
US6111166A (en) | 1994-09-19 | 2000-08-29 | Medarex, Incorporated | Transgenic mice expressing human Fcα and β receptors |
US5880327A (en) | 1994-09-21 | 1999-03-09 | American National Red Cross | Transgenic mammals expressing human coagulation factor VIII |
US5556752A (en) | 1994-10-24 | 1996-09-17 | Affymetrix, Inc. | Surface-bound, unimolecular, double-stranded DNA |
US6187992B1 (en) | 1994-12-05 | 2001-02-13 | Merck & Co., Inc. | Transgenic mouse having a disrupted amyloid precursor protein gene |
US5830645A (en) | 1994-12-09 | 1998-11-03 | The Regents Of The University Of California | Comparative fluorescence hybridization to nucleic acid arrays |
US5633440A (en) | 1994-12-20 | 1997-05-27 | Dna Plant Technology Corporation | P119 promoters and their uses |
US6093562A (en) * | 1996-02-05 | 2000-07-25 | Novo Nordisk A/S | Amylase variants |
US5959098A (en) | 1996-04-17 | 1999-09-28 | Affymetrix, Inc. | Substrate preparation process |
US6313081B1 (en) | 1995-04-28 | 2001-11-06 | Henkel Kommanditgesellschaft Auf Aktien (Kgaa) | Detergents comprising cellulases |
US5552317A (en) * | 1995-05-26 | 1996-09-03 | Industrial Technology Research Institute | Method for preparing optically active homophenylalanine and esters thereof using lipase from wheat germ or Candida lipolytica |
WO1996039154A1 (en) | 1995-06-06 | 1996-12-12 | Isis Pharmaceuticals, Inc. | Oligonucleotides having phosphorothioate linkages of high chiral purity |
WO1997001629A1 (en) | 1995-06-28 | 1997-01-16 | Novo Nordisk A/S | A cellulase with reduced mobility |
US5856174A (en) | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
US5985662A (en) | 1995-07-13 | 1999-11-16 | Isis Pharmaceuticals Inc. | Antisense inhibition of hepatitis B virus replication |
US5958672A (en) | 1995-07-18 | 1999-09-28 | Diversa Corporation | Protein activity screening of clones having DNA from uncultivated microorganisms |
WO1997016533A1 (en) | 1995-10-31 | 1997-05-09 | The Regents Of The University Of California | Mammalian artificial chromosomes and methods of using same |
US6764835B2 (en) | 1995-12-07 | 2004-07-20 | Diversa Corporation | Saturation mutageneis in directed evolution |
US6361974B1 (en) | 1995-12-07 | 2002-03-26 | Diversa Corporation | Exonuclease-mediated nucleic acid reassembly in directed evolution |
US6740506B2 (en) | 1995-12-07 | 2004-05-25 | Diversa Corporation | End selection in directed evolution |
US6171820B1 (en) * | 1995-12-07 | 2001-01-09 | Diversa Corporation | Saturation mutagenesis in directed evolution |
US6358709B1 (en) | 1995-12-07 | 2002-03-19 | Diversa Corporation | End selection in directed evolution |
US5830696A (en) | 1996-12-05 | 1998-11-03 | Diversa Corporation | Directed evolution of thermophilic enzymes |
US6238884B1 (en) | 1995-12-07 | 2001-05-29 | Diversa Corporation | End selection in directed evolution |
US6562594B1 (en) | 1999-09-29 | 2003-05-13 | Diversa Corporation | Saturation mutagenesis in directed evolution |
US5939250A (en) | 1995-12-07 | 1999-08-17 | Diversa Corporation | Production of enzymes having desired activities by mutagenesis |
US5965408A (en) | 1996-07-09 | 1999-10-12 | Diversa Corporation | Method of DNA reassembly by interrupting synthesis |
US6537776B1 (en) | 1999-06-14 | 2003-03-25 | Diversa Corporation | Synthetic ligation reassembly in directed evolution |
US6022963A (en) | 1995-12-15 | 2000-02-08 | Affymetrix, Inc. | Synthesis of oligonucleotide arrays using photocleavable protecting groups |
US6013440A (en) | 1996-03-11 | 2000-01-11 | Affymetrix, Inc. | Nucleic acid affinity columns |
US6096548A (en) | 1996-03-25 | 2000-08-01 | Maxygen, Inc. | Method for directing evolution of a virus |
US6025155A (en) | 1996-04-10 | 2000-02-15 | Chromos Molecular Systems, Inc. | Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes |
US6197070B1 (en) * | 1996-05-15 | 2001-03-06 | The Procter & Gamble Company | Detergent compositions comprising alpha combination of α-amylases for malodor stripping |
WO1997046313A1 (en) | 1996-06-07 | 1997-12-11 | Eos Biotechnology, Inc. | Immobilised linear oligonucleotide arrays |
BR9710635A (pt) * | 1996-07-30 | 1999-08-17 | Procter & Gamble | Composi-Æo detergente compreendendo dois componentesde celulase com e sem um dominio de liga-Æo - celulose |
US6326341B1 (en) * | 1996-09-11 | 2001-12-04 | The Procter & Gamble Company | Low foaming automatic dishwashing compositions |
US5834259A (en) * | 1996-10-28 | 1998-11-10 | Monsanto Company | Process and composition for preparing D-aspartic acid |
PL334336A1 (en) * | 1996-12-19 | 2000-02-28 | Unilever Nv | Immobilised enzyme and application thereof in processing triglyceride oils |
US6069122A (en) | 1997-06-16 | 2000-05-30 | The Procter & Gamble Company | Dishwashing detergent compositions containing organic diamines for improved grease cleaning, sudsing, low temperature stability and dissolution |
DE69835360T2 (de) | 1997-01-17 | 2007-08-16 | Maxygen, Inc., Redwood City | EVOLUTION Prokaryotischer GANZER ZELLEN DURCH REKURSIVE SEQUENZREKOMBINATION |
US6326204B1 (en) | 1997-01-17 | 2001-12-04 | Maxygen, Inc. | Evolution of whole cells and organisms by recursive sequence recombination |
DE19703364A1 (de) * | 1997-01-30 | 1998-08-06 | Henkel Ecolab Gmbh & Co Ohg | Pastenförmiges Wasch- und Reinigungsmittel |
BR9808368A (pt) | 1997-03-18 | 2000-05-23 | Novo Nordisk As | Processos para a construção de uma biblioteca de polinucleotideos homólogos recombinados, para a identificação um polipeptìdeo de interesse, e, para a produção de um polipeptìdeo de interesse. |
AU6611498A (en) | 1997-03-18 | 1998-10-12 | Novo Nordisk A/S | Method for constructing a library using dna shuffling |
US5948653A (en) | 1997-03-21 | 1999-09-07 | Pati; Sushma | Sequence alterations using homologous recombination |
US6153410A (en) | 1997-03-25 | 2000-11-28 | California Institute Of Technology | Recombination of polynucleotide sequences using random or defined primers |
US6826296B2 (en) | 1997-07-25 | 2004-11-30 | Affymetrix, Inc. | Method and system for providing a probe array chip design database |
JP2001514906A (ja) | 1997-08-15 | 2001-09-18 | ハイセック,インコーポレーテッド | 核酸種を検出または定量するための方法および組成物 |
WO1999013313A1 (en) | 1997-09-11 | 1999-03-18 | Genovations, Inc. | Method of making high density arrays |
US6465178B2 (en) | 1997-09-30 | 2002-10-15 | Surmodics, Inc. | Target molecule attachment to surfaces |
US6399383B1 (en) | 1997-10-28 | 2002-06-04 | Maxygen, Inc. | Human papilloma virus vectors |
DE69842027D1 (de) * | 1997-10-30 | 2011-01-13 | Novozymes As | Mutanten der alpha-amylase |
EP1030861A4 (en) | 1997-10-31 | 2001-09-05 | Maxygen Inc | MODIFICATION OF VIRAL TROPISM AND THE DIVERSITY OF HOST SPECIES BY RECOMBINATION OF THE VIRAL GENOME |
ATE234913T1 (de) * | 1997-11-10 | 2003-04-15 | Procter & Gamble | Verfahren zur herstellung einer waschmitteltablette |
DE69828574T2 (de) | 1997-11-14 | 2005-10-06 | Sankyo Co., Ltd. | Transgenes Tier als Modell für Allergie und seine Verwendung |
US6537746B2 (en) | 1997-12-08 | 2003-03-25 | Maxygen, Inc. | Method for creating polynucleotide and polypeptide sequences |
US6506559B1 (en) | 1997-12-23 | 2003-01-14 | Carnegie Institute Of Washington | Genetic inhibition by double-stranded RNA |
CA2320958A1 (en) | 1998-02-11 | 1999-08-19 | Maxygen, Inc. | Antigen library immunization |
CA2320431A1 (en) | 1998-02-11 | 1999-08-19 | Maxygen, Inc. | Targeting of genetic vaccine vectors |
IL138668A0 (en) | 1998-04-03 | 2001-10-31 | Phylos Inc | Addressable protein arrays |
US6156952A (en) | 1998-04-09 | 2000-12-05 | Constituent Institution Of The University Of Maryland System | HIV transgenic animals and uses therefor |
EP1082482A1 (en) * | 1998-05-01 | 2001-03-14 | Novozymes A/S | Enhancers such as n-hydroxyacetanilide |
US6048695A (en) | 1998-05-04 | 2000-04-11 | Baylor College Of Medicine | Chemically modified nucleic acids and methods for coupling nucleic acids to solid support |
DE19824705A1 (de) * | 1998-06-03 | 1999-12-09 | Henkel Kgaa | Amylase und Protease enthaltende Wasch- und Reinigungsmittel |
US6022567A (en) | 1998-06-23 | 2000-02-08 | Nestec S.A. | Flavor enhancer |
ATE547532T1 (de) | 1998-06-29 | 2012-03-15 | Bristol Myers Squibb Co | Verfahren zur erzeugung von hochgradig diversen bibliotheken |
JP4335995B2 (ja) * | 1998-07-22 | 2009-09-30 | 昭 神谷 | 環境保全型粒状洗浄用組成物 |
FR2782323B1 (fr) | 1998-08-12 | 2002-01-11 | Proteus | Procede de production in vitro de sequences polynucleotidiques recombinees, banques de sequences et sequences ainsi obtenues |
GB2340755B (en) | 1998-08-24 | 2002-09-25 | Cannon Rubber Ltd | Breast pump insert |
US6277628B1 (en) | 1998-10-02 | 2001-08-21 | Incyte Genomics, Inc. | Linear microarrays |
US6277489B1 (en) | 1998-12-04 | 2001-08-21 | The Regents Of The University Of California | Support for high performance affinity chromatography and other uses |
US6368861B1 (en) | 1999-01-19 | 2002-04-09 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
US6376246B1 (en) | 1999-02-05 | 2002-04-23 | Maxygen, Inc. | Oligonucleotide mediated nucleic acid recombination |
US6436675B1 (en) | 1999-09-28 | 2002-08-20 | Maxygen, Inc. | Use of codon-varied oligonucleotide synthesis for synthetic shuffling |
US7024312B1 (en) | 1999-01-19 | 2006-04-04 | Maxygen, Inc. | Methods for making character strings, polynucleotides and polypeptides having desired characteristics |
US6399121B1 (en) * | 1999-03-16 | 2002-06-04 | Novozymes A/S | Process for producing cheese |
DE60018267T3 (de) | 1999-03-16 | 2014-02-06 | Novozymes A/S | Verfahren zur herstellung von käse |
US6511824B1 (en) | 1999-03-17 | 2003-01-28 | Exelixis, Inc. | Nucleic acids and polypeptides of invertebrate TWIK channels and methods of use |
US6309871B1 (en) * | 1999-03-31 | 2001-10-30 | Novozymes A/S | Polypeptides having alkaline α-amylase activity |
US6221653B1 (en) | 1999-04-27 | 2001-04-24 | Agilent Technologies, Inc. | Method of performing array-based hybridization assays using thermal inkjet deposition of sample fluids |
US6653151B2 (en) | 1999-07-30 | 2003-11-25 | Large Scale Proteomics Corporation | Dry deposition of materials for microarrays using matrix displacement |
US6337187B1 (en) | 1999-11-05 | 2002-01-08 | Millennium Pharmaceuticals, Inc. | 18891, a novel human lipase |
US20010008765A1 (en) | 1999-12-06 | 2001-07-19 | Fuji Photo Film Co., Ltd. | DNA chip and reactive solid carrier |
US6531644B1 (en) | 2000-01-14 | 2003-03-11 | Exelixis, Inc. | Methods for identifying anti-cancer drug targets |
AU1166502A (en) | 2000-10-12 | 2002-04-22 | Exelixis Inc | Human ect2 and methods of use |
US6309872B1 (en) | 2000-11-01 | 2001-10-30 | Novozymes Biotech, Inc | Polypeptides having glucoamylase activity and nucleic acids encoding same |
MXPA04007886A (es) * | 2002-02-13 | 2004-10-15 | Dow Global Technologies Inc | Sobre-expresion de genes de extremozimo en pseudomonadas y bacterias estrechamente relacionadas. |
WO2003070013A1 (en) | 2002-02-20 | 2003-08-28 | Novozymes A/S | Process for producing cheese |
BRPI0416744A (pt) * | 2003-11-19 | 2007-01-16 | Dow Global Technologies Inc | sistemas de expressão de proteìna aperfeiçoados |
EP1774017B1 (en) | 2004-07-26 | 2013-05-15 | Pfenex Inc. | Process for improved protein expression by strain engineering |
US8569015B2 (en) * | 2006-05-30 | 2013-10-29 | Pfenex Inc. | RPA optimization |
UA97127C2 (ru) * | 2006-12-06 | 2012-01-10 | Бандж Ойлз, Инк. | Способ непрерывной ферментативной обработки композиции, содержащей липид, и система для его осуществления |
CN101821402B (zh) | 2007-07-31 | 2015-03-04 | Bp法人北美有限公司 | 修剪式多位点组合装配 |
-
2008
- 2008-08-29 US US12/202,119 patent/US8198062B2/en active Active
-
2009
- 2009-08-28 BR BRPI0918265A patent/BRPI0918265A8/pt not_active Application Discontinuation
- 2009-08-28 CA CA2735230A patent/CA2735230C/en active Active
- 2009-08-28 CA CA3060541A patent/CA3060541C/en active Active
- 2009-08-28 AR ARP090103327A patent/AR073942A1/es not_active Application Discontinuation
- 2009-08-28 KR KR1020117007310A patent/KR20110050703A/ko not_active Application Discontinuation
- 2009-08-28 WO PCT/US2009/055412 patent/WO2010025395A2/en active Application Filing
- 2009-08-28 ES ES09810676T patent/ES2714673T3/es active Active
- 2009-08-28 CN CN200980142971.7A patent/CN102197043B/zh active Active
- 2009-08-28 MX MX2011002297A patent/MX2011002297A/es active IP Right Grant
- 2009-08-28 EP EP09810676.8A patent/EP2328907B1/en active Active
- 2009-08-28 EA EA201170388A patent/EA020145B9/ru not_active IP Right Cessation
-
2012
- 2012-05-14 US US13/471,206 patent/US9238804B2/en active Active
-
2015
- 2015-12-10 US US14/964,708 patent/US20160108383A1/en not_active Abandoned
-
2017
- 2017-11-07 US US15/805,269 patent/US20180057803A1/en not_active Abandoned
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20070202566A1 (en) * | 2003-03-07 | 2007-08-30 | Bornscheuer Uwe T | Hydrolases, Nucleic Acids Encoding Them And Methods For Making And Using Them |
Non-Patent Citations (1)
Title |
---|
SCHMITT ET AL., Blocking the tunnel: engineering of Candida rugosa lipase mutants with short chain length specificity, Protein Engineering, July, 2002, vol. 15, no 7, p. 595-601; abstract * |
Also Published As
Publication number | Publication date |
---|---|
US20100055085A1 (en) | 2010-03-04 |
EA020145B1 (ru) | 2014-09-30 |
CA3060541A1 (en) | 2010-03-04 |
EP2328907A2 (en) | 2011-06-08 |
WO2010025395A3 (en) | 2010-04-22 |
CA3060541C (en) | 2021-08-10 |
EP2328907A4 (en) | 2012-03-21 |
US9238804B2 (en) | 2016-01-19 |
CN102197043A (zh) | 2011-09-21 |
ES2714673T3 (es) | 2019-05-29 |
CA2735230A1 (en) | 2010-03-04 |
US20180057803A1 (en) | 2018-03-01 |
MX2011002297A (es) | 2011-05-25 |
WO2010025395A2 (en) | 2010-03-04 |
KR20110050703A (ko) | 2011-05-16 |
EP2328907B1 (en) | 2018-12-12 |
AR073942A1 (es) | 2010-12-15 |
CA2735230C (en) | 2019-12-24 |
US20160108383A1 (en) | 2016-04-21 |
US20120227120A1 (en) | 2012-09-06 |
US8198062B2 (en) | 2012-06-12 |
EA201170388A1 (ru) | 2011-10-31 |
CN102197043B (zh) | 2018-01-23 |
BRPI0918265A2 (pt) | 2017-10-31 |
BRPI0918265A8 (pt) | 2018-01-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
MY162662A (en) | Hydrolases, nucleic acids encoding them and methods for making and using them | |
EA020145B9 (ru) | Гидролазы, кодирующие их нуклеиновые кислоты и способы их получения и применения | |
EA201171291A1 (ru) | Кормовая добавка | |
IN2012DN03825A (ru) | ||
MX2016001315A (es) | Variantes de enzimas. | |
MX364988B (es) | Composición de alimento animal que comprende variantes de fitasa de buttiauxella sp. | |
MY187676A (en) | Microorganisms for the production of 1,4-butanediol and related methods | |
MX2009010574A (es) | Multienzimas y su uso en la fabricacion de acidos grasos poliinsaturados. | |
AU2007356171A8 (en) | Glucanases, nucleic acids encoding them, and methods for making and using them | |
EP1989301A4 (en) | CELLULOLYTIC ENZYMES, NUCLEIC ACIDS ENCODING SAME, AND METHODS FOR PREPARING AND USING SAME | |
MY160100A (en) | Beta-glucosidase variants having improved activity, and uses thereof | |
WO2011079314A8 (en) | Compositions and methods for the increased production of isoprene and other products with 6 - phosphogluconolactonase (pgl) | |
PH12013501799A1 (en) | Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials | |
MY195138A (en) | Microorganisms and Methods for Production of Specific Length Fatty Alcohols and Related Compounds | |
UY30826A1 (es) | Nuevo gen bacillus thuringiensis con actividad contra lepidópteros | |
CL2011001462A1 (es) | Yarrowia sp. transgenica util en la fermentacion de acidos grasos poliinsaturados que comprende un polipeptido con actividad malonil-coa sintetasa; y metodo para reducir la produccion de malonatos como subproducto de la fermetacion basado en el uso de dicha yarrowia. | |
WO2011078601A3 (ko) | 음식물 쓰레기 처리용 혼합 균주 및 이를 이용한 음식물 쓰레기 처리 방법 | |
MX336787B (es) | Microorganismos deseñados geneticamente que comprenden 4-hidroxibenzoil-coa tioesterasas y metodos de uso de los mismos para producir acidos grasos libes y derivados de acidos grasos. | |
AR070143A1 (es) | Gen de bacillus thuringiensis (bt) con actividad contra coleopteros | |
MX2013000758A (es) | Plantas que tienen rasgos mejorados relacionados con el rendimiento y un metodo para llevarlo acabo. | |
WO2008019069A3 (en) | Enzymatic aqueous acylation | |
EP2865748A4 (en) | CULTURE MEDIUM FOR BACTERIA OF THE GENUS CLOSTRIDIUM FREE OF ANIMAL CONSTITUENTS AND PROCESS FOR PRODUCING A SURNAGEANT CONTAINING ONE OR MORE PROTEASES WITH COLLAGENOLYTIC AND GELATINOLYTIC ACTIVITY | |
WO2008079545A3 (en) | Generation of plants with altered protein, fiber, or oil content | |
WO2008106662A3 (en) | Nitrilases, nucleic acids encoding them and methods for making and using them | |
WO2008061153A3 (en) | Generation of plants with altered protein, fiber, or oil content |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
TH4A | Publication of the corrected specification to eurasian patent | ||
MM4A | Lapse of a eurasian patent due to non-payment of renewal fees within the time limit in the following designated state(s) |
Designated state(s): AM AZ BY KZ KG MD TJ TM |