DK3215638T3 - Sekventering fra flere primere til forøgelse af datahastigheden og -densiteten - Google Patents

Sekventering fra flere primere til forøgelse af datahastigheden og -densiteten Download PDF

Info

Publication number
DK3215638T3
DK3215638T3 DK15794241.8T DK15794241T DK3215638T3 DK 3215638 T3 DK3215638 T3 DK 3215638T3 DK 15794241 T DK15794241 T DK 15794241T DK 3215638 T3 DK3215638 T3 DK 3215638T3
Authority
DK
Denmark
Prior art keywords
sequencing
density
data rate
increase data
multiple primers
Prior art date
Application number
DK15794241.8T
Other languages
Danish (da)
English (en)
Inventor
Jonathan Mark Boutell
Original Assignee
Illumina Cambridge Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Cambridge Ltd filed Critical Illumina Cambridge Ltd
Application granted granted Critical
Publication of DK3215638T3 publication Critical patent/DK3215638T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G06T7/0012—Biomedical image inspection
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/30—Subject of image; Context of image processing
    • G06T2207/30004—Biomedical image processing
    • G06T2207/30072—Microarray; Biochip, DNA array; Well plate

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medical Informatics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Radiology & Medical Imaging (AREA)
  • Quality & Reliability (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • General Physics & Mathematics (AREA)
  • Theoretical Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
DK15794241.8T 2014-11-05 2015-11-04 Sekventering fra flere primere til forøgelse af datahastigheden og -densiteten DK3215638T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1419731.3A GB201419731D0 (en) 2014-11-05 2014-11-05 Sequencing from multiple primers to increase data rate and density
PCT/GB2015/053324 WO2016071689A1 (en) 2014-11-05 2015-11-04 Sequencing from multiple primers to increase data rate and density

Publications (1)

Publication Number Publication Date
DK3215638T3 true DK3215638T3 (da) 2021-10-18

Family

ID=52118764

Family Applications (1)

Application Number Title Priority Date Filing Date
DK15794241.8T DK3215638T3 (da) 2014-11-05 2015-11-04 Sekventering fra flere primere til forøgelse af datahastigheden og -densiteten

Country Status (11)

Country Link
US (3) US20170298430A1 (enExample)
EP (2) EP3215638B1 (enExample)
JP (1) JP6789935B2 (enExample)
CN (2) CN113564238B (enExample)
AU (2) AU2015341568B2 (enExample)
CA (1) CA2966442C (enExample)
DK (1) DK3215638T3 (enExample)
ES (1) ES2889875T3 (enExample)
GB (1) GB201419731D0 (enExample)
SG (1) SG11201703629XA (enExample)
WO (1) WO2016071689A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2017258619B2 (en) 2016-04-29 2020-05-14 Pacific Biosciences Of California, Inc. Sequencing method employing ternary complex destabilization to identify cognate nucleotides
US10428378B2 (en) 2016-08-15 2019-10-01 Omniome, Inc. Sequencing method for rapid identification and processing of cognate nucleotide pairs
EP3519586B1 (en) * 2016-09-28 2024-03-13 Life Technologies Corporation Methods for sequencing nucleic acids using termination chemistry
EP3562962B1 (en) 2016-12-30 2022-01-05 Omniome, Inc. Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides
DE102017218849A1 (de) * 2017-10-23 2019-04-25 Robert Bosch Gmbh Reaktionsträger für eine mikrofluidische Vorrichtung und Verfahren zur Bestimmung einer Nukleotidsequenz
CN113249455A (zh) * 2020-02-12 2021-08-13 赛纳生物科技(北京)有限公司 一种基因测序中获得背景信号的方法
WO2023114392A1 (en) * 2021-12-16 2023-06-22 Ultima Genomics, Inc. Systems and methods for sequencing with multi-priming
KR20240162122A (ko) * 2022-03-15 2024-11-14 일루미나, 인코포레이티드 병렬 샘플 및 인덱스 시퀀싱
CA3223669A1 (en) * 2022-03-15 2023-09-21 Niall Gormley Concurrent sequencing of forward and reverse complement strands on concatenated polynucleotides for methylation detection
CN115232867B (zh) * 2022-07-21 2024-01-30 深圳赛陆医疗科技有限公司 一种快速的二代基因测序方法
CN120418443A (zh) * 2022-12-12 2025-08-01 深圳华大智造科技股份有限公司 一种多模板核酸同步测序的方法及其应用
CN117721191B (zh) * 2024-02-07 2024-05-10 深圳赛陆医疗科技有限公司 基因测序方法、测序装置、可读存储介质和基因测序系统

Family Cites Families (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU622426B2 (en) 1987-12-11 1992-04-09 Abbott Laboratories Assay using template-dependent nucleic acid probe reorganization
CA1341584C (en) 1988-04-06 2008-11-18 Bruce Wallace Method of amplifying and detecting nucleic acid sequences
WO1989009835A1 (en) 1988-04-08 1989-10-19 The Salk Institute For Biological Studies Ligase-based amplification method
ATE144556T1 (de) 1988-06-24 1996-11-15 Amgen Inc Verfahren und mittel zum nachweis von nukleinsäuresequenzen
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
WO1990001069A1 (en) 1988-07-20 1990-02-08 Segev Diagnostics, Inc. Process for amplifying and detecting nucleic acid sequences
US5185243A (en) 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
US5573907A (en) 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
DE69118930T2 (de) 1990-01-26 1997-01-09 Abbott Lab Verbessertes Verfahren zur Amplifikation von Nuklein säurezielsequenz, einsetzbar für die Polymerase und Ligasekettenreaktion
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
AU694187B2 (en) 1994-02-07 1998-07-16 Beckman Coulter, Inc. Ligase/polymerase-mediated genetic bit analysis TM of single nucleotide polymorphisms and its use in genetic analysis
KR100230718B1 (ko) 1994-03-16 1999-11-15 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 등온 가닥 변위 핵산 증폭법
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
DE19515552A1 (de) * 1995-04-27 1996-10-31 Europ Lab Molekularbiolog Simultane Sequenzierung von Nukleinsäuren
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
EP1591541B1 (en) 1997-04-01 2012-02-15 Illumina Cambridge Limited Method of nucleic acid sequencing
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
WO2001027327A2 (en) * 1999-10-08 2001-04-19 Protogene Laboratories, Inc. Method and apparatus for performing large numbers of reactions using array assembly
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
AU2001282881B2 (en) 2000-07-07 2007-06-14 Visigen Biotechnologies, Inc. Real-time sequence determination
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
EP1472335A4 (en) * 2001-11-08 2005-12-28 Univ Johns Hopkins METHODS AND SYSTEMS FOR SEQUENCING NUCLEIC ACIDS
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
EP3795577A1 (en) 2002-08-23 2021-03-24 Illumina Cambridge Limited Modified nucleotides
WO2005003304A2 (en) 2003-06-20 2005-01-13 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
US7169560B2 (en) * 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
JP2007525571A (ja) 2004-01-07 2007-09-06 ソレクサ リミテッド 修飾分子アレイ
WO2006044078A2 (en) 2004-09-17 2006-04-27 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
WO2006064199A1 (en) 2004-12-13 2006-06-22 Solexa Limited Improved method of nucleotide detection
JP4990886B2 (ja) 2005-05-10 2012-08-01 ソレックサ リミテッド 改良ポリメラーゼ
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
WO2007123744A2 (en) 2006-03-31 2007-11-01 Solexa, Inc. Systems and devices for sequence by synthesis analysis
US7754429B2 (en) * 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
US8343746B2 (en) 2006-10-23 2013-01-01 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
EP2639579B1 (en) 2006-12-14 2016-11-16 Life Technologies Corporation Apparatus for measuring analytes using large scale FET arrays
WO2008093098A2 (en) * 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
WO2009054922A1 (en) * 2007-10-19 2009-04-30 The Trustees Of Columbia University In The City Of New York Dna sequencing with non-fluorescent nucleotide reversible terminators and cleavable label modified nucleotide terminators
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20110195410A1 (en) * 2009-12-07 2011-08-11 Gene Check, Inc. Compositions and methods related to solid phase sequence detection and genotyping
CN102140534A (zh) * 2010-12-15 2011-08-03 深圳华大基因科技有限公司 一种hbv基因的核苷酸突变位点的检测方法
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
US11130992B2 (en) * 2011-03-31 2021-09-28 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable multiplex COLD-PCR
US20120252682A1 (en) * 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
RS62530B1 (sr) 2011-09-23 2021-11-30 Illumina Inc Sastavi za sekvenciranje nukleinskih kiselina
AU2013243998B2 (en) 2012-04-03 2016-07-28 Illumina, Inc. Integrated optoelectronic read head and fluidic cartridge useful for nucleic acid sequencing
CN109112193A (zh) * 2013-07-03 2019-01-01 伊鲁米那股份有限公司 正交合成测序

Also Published As

Publication number Publication date
US20200392573A1 (en) 2020-12-17
CA2966442A1 (en) 2016-05-12
ES2889875T3 (es) 2022-01-14
WO2016071689A1 (en) 2016-05-12
GB201419731D0 (en) 2014-12-17
CA2966442C (en) 2024-03-12
CN113564238A (zh) 2021-10-29
EP3215638A1 (en) 2017-09-13
AU2021286342B2 (en) 2024-05-30
EP3974538A1 (en) 2022-03-30
SG11201703629XA (en) 2017-06-29
AU2021286342A1 (en) 2022-01-20
CN107257862B (zh) 2021-08-03
CN107257862A (zh) 2017-10-17
EP3215638B1 (en) 2021-08-11
JP2017533709A (ja) 2017-11-16
HK1243465A1 (en) 2018-07-13
JP6789935B2 (ja) 2020-11-25
AU2015341568B2 (en) 2021-09-16
US20170298430A1 (en) 2017-10-19
US20230160006A1 (en) 2023-05-25
AU2015341568A1 (en) 2017-05-25
US11555218B2 (en) 2023-01-17
CN113564238B (zh) 2025-08-05

Similar Documents

Publication Publication Date Title
DK3215638T3 (da) Sekventering fra flere primere til forøgelse af datahastigheden og -densiteten
DK3102722T3 (da) Genom fraktionering
DK3155101T3 (da) Sammensætninger og fremgangsmåder til ekspression af CRISPR-leder-RNA´er under anvendelse af H1-promotoren
DK3161154T3 (da) Modificerede polymeraser til forbedret inkorporering af nukleotidanaloger
DK3188836T3 (da) Dna-syntese
EP3218800C0 (en) SECURE MULTI-PARTY CALCULATION ON SPREADSHEETS
DK3134506T3 (da) Fremgangsmåder til oprensning af messenger rna
DK3116533T3 (da) Deletion af dystrophingenexon under anvendelse af genmanipulerede nukleaser
DK3123260T3 (da) Selektiv behandling af sensordata
DK3149712T3 (da) Køsystem
SG11201610168YA (en) Nucleic acid synthesis techniques
UA31653S (uk) Курильне приладдя
DK3144001T3 (da) Sammensætninger af selenoorganiske forbindelser og fremgangsmåder til anvendelse deraf
DK3189283T3 (da) Filterhus
EP3315430A4 (en) CONTAINER
DK3143123T3 (da) Mikrorna-induktion af kardiologisk regenerering
DE112015003457A5 (de) Al-Gusslegierung
IL249254A0 (en) Gene expression system
PL3206764T3 (pl) Rakieta śnieżna
DK3105873T3 (da) Teknik til lagring af softbits
DK3146147T3 (da) Udvinding af skifergas
DK3094551T3 (da) Redningsfartøj
IL252086A0 (en) Novel methods
EP3174785C0 (en) RECOVERY SYSTEM
DK3131389T3 (da) Forbedret fremgangsmåde til østersavl