CN113564238B - 从多个引物测序以增加数据速率和密度 - Google Patents

从多个引物测序以增加数据速率和密度

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Publication number
CN113564238B
CN113564238B CN202110829488.9A CN202110829488A CN113564238B CN 113564238 B CN113564238 B CN 113564238B CN 202110829488 A CN202110829488 A CN 202110829488A CN 113564238 B CN113564238 B CN 113564238B
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signal
primers
nucleotide
perform
processor
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CN113564238A (zh
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J.M.鲍特尔
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Illumina Cambridge Ltd
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Illumina Cambridge Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • C12Q1/6874Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00Image analysis
    • G06T7/0002Inspection of images, e.g. flaw detection
    • G06T7/0012Biomedical image inspection
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00Indexing scheme for image analysis or image enhancement
    • G06T2207/30Subject of image; Context of image processing
    • G06T2207/30004Biomedical image processing
    • G06T2207/30072Microarray; Biochip, DNA array; Well plate

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Medical Informatics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Radiology & Medical Imaging (AREA)
  • Quality & Reliability (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • General Physics & Mathematics (AREA)
  • Theoretical Computer Science (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Chemical Kinetics & Catalysis (AREA)
CN202110829488.9A 2014-11-05 2015-11-04 从多个引物测序以增加数据速率和密度 Active CN113564238B (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
GBGB1419731.3A GB201419731D0 (en) 2014-11-05 2014-11-05 Sequencing from multiple primers to increase data rate and density
GB1419731.3 2014-11-05
CN201580069430.1A CN107257862B (zh) 2014-11-05 2015-11-04 从多个引物测序以增加数据速率和密度
PCT/GB2015/053324 WO2016071689A1 (en) 2014-11-05 2015-11-04 Sequencing from multiple primers to increase data rate and density

Related Parent Applications (1)

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CN201580069430.1A Division CN107257862B (zh) 2014-11-05 2015-11-04 从多个引物测序以增加数据速率和密度

Publications (2)

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CN113564238A CN113564238A (zh) 2021-10-29
CN113564238B true CN113564238B (zh) 2025-08-05

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CN201580069430.1A Active CN107257862B (zh) 2014-11-05 2015-11-04 从多个引物测序以增加数据速率和密度

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US (3) US20170298430A1 (enExample)
EP (2) EP3215638B1 (enExample)
JP (1) JP6789935B2 (enExample)
CN (2) CN113564238B (enExample)
AU (2) AU2015341568B2 (enExample)
CA (1) CA2966442C (enExample)
DK (1) DK3215638T3 (enExample)
ES (1) ES2889875T3 (enExample)
GB (1) GB201419731D0 (enExample)
SG (1) SG11201703629XA (enExample)
WO (1) WO2016071689A1 (enExample)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10294514B2 (en) 2016-04-29 2019-05-21 Omniome, Inc. Sequencing method employing ternary complex destabilization to identify cognate nucleotides
US10428378B2 (en) 2016-08-15 2019-10-01 Omniome, Inc. Sequencing method for rapid identification and processing of cognate nucleotide pairs
WO2018064311A2 (en) * 2016-09-28 2018-04-05 Life Technologies Corporation Methods and systems for reducing phasing errors when sequencing nucleic acids using termination chemistry
US11248254B2 (en) 2016-12-30 2022-02-15 Omniome, Inc. Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides
DE102017218849A1 (de) * 2017-10-23 2019-04-25 Robert Bosch Gmbh Reaktionsträger für eine mikrofluidische Vorrichtung und Verfahren zur Bestimmung einer Nukleotidsequenz
CN113249455A (zh) * 2020-02-12 2021-08-13 赛纳生物科技(北京)有限公司 一种基因测序中获得背景信号的方法
WO2023114392A1 (en) * 2021-12-16 2023-06-22 Ultima Genomics, Inc. Systems and methods for sequencing with multi-priming
EP4493721A1 (en) * 2022-03-15 2025-01-22 Illumina, Inc. Parallel sample and index sequencing
WO2023175037A2 (en) * 2022-03-15 2023-09-21 Illumina, Inc. Concurrent sequencing of forward and reverse complement strands on separate polynucleotides for methylation detection
CN115232867B (zh) * 2022-07-21 2024-01-30 深圳赛陆医疗科技有限公司 一种快速的二代基因测序方法
CN120418443A (zh) * 2022-12-12 2025-08-01 深圳华大智造科技股份有限公司 一种多模板核酸同步测序的方法及其应用
CN117721191B (zh) * 2024-02-07 2024-05-10 深圳赛陆医疗科技有限公司 基因测序方法、测序装置、可读存储介质和基因测序系统

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5976802A (en) * 1995-04-27 1999-11-02 Europasches Laboratorium fur Molekularbiologie (EMBL) Simultaneous sequencing of nucleic acids

Family Cites Families (57)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
CA1341584C (en) 1988-04-06 2008-11-18 Bruce Wallace Method of amplifying and detecting nucleic acid sequences
AU3539089A (en) 1988-04-08 1989-11-03 Salk Institute For Biological Studies, The Ligase-based amplification method
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
ATE144556T1 (de) 1988-06-24 1996-11-15 Amgen Inc Verfahren und mittel zum nachweis von nukleinsäuresequenzen
JP2955759B2 (ja) 1988-07-20 1999-10-04 セゲブ・ダイアグノスティックス・インコーポレイテッド 核酸配列を増幅及び検出する方法
US5185243A (en) 1988-08-25 1993-02-09 Syntex (U.S.A.) Inc. Method for detection of specific nucleic acid sequences
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
ES2089038T3 (es) 1990-01-26 1996-10-01 Abbott Lab Procedimiento mejorado para amplificar acidos nucleicos blanco aplicable para la reaccion en cadena de polimerasa y ligasa.
US5573907A (en) 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
WO1995021271A1 (en) 1994-02-07 1995-08-10 Molecular Tool, Inc. Ligase/polymerase-mediated genetic bit analysistm of single nucleotide polymorphisms and its use in genetic analysis
JPH09510351A (ja) 1994-03-16 1997-10-21 ジェン−プローブ・インコーポレイテッド 等温鎖置換核酸増幅法
US5846719A (en) 1994-10-13 1998-12-08 Lynx Therapeutics, Inc. Oligonucleotide tags for sorting and identification
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
ATE364718T1 (de) 1997-04-01 2007-07-15 Solexa Ltd Verfahren zur vervielfältigung von nukleinsäure
US6969488B2 (en) 1998-05-22 2005-11-29 Solexa, Inc. System and apparatus for sequential processing of analytes
AR021833A1 (es) 1998-09-30 2002-08-07 Applied Research Systems Metodos de amplificacion y secuenciacion de acido nucleico
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
EP1235932A2 (en) * 1999-10-08 2002-09-04 Protogene Laboratories, Inc. Method and apparatus for performing large numbers of reactions using array assembly
US7955794B2 (en) 2000-09-21 2011-06-07 Illumina, Inc. Multiplex nucleic acid reactions
US7582420B2 (en) 2001-07-12 2009-09-01 Illumina, Inc. Multiplex nucleic acid reactions
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US7001792B2 (en) 2000-04-24 2006-02-21 Eagle Research & Development, Llc Ultra-fast nucleic acid sequencing device and a method for making and using the same
JP2004513619A (ja) 2000-07-07 2004-05-13 ヴィジゲン バイオテクノロジーズ インコーポレイテッド リアルタイム配列決定
WO2002044425A2 (en) 2000-12-01 2002-06-06 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
WO2003056030A2 (en) * 2001-11-08 2003-07-10 The Johns Hopkins University Methods and systems of nucleic acid sequencing
US7057026B2 (en) 2001-12-04 2006-06-06 Solexa Limited Labelled nucleotides
SI3363809T1 (sl) 2002-08-23 2020-08-31 Illumina Cambridge Limited Modificirani nukleotidi za polinukleotidno sekvenciranje
WO2005003304A2 (en) 2003-06-20 2005-01-13 Illumina, Inc. Methods and compositions for whole genome amplification and genotyping
GB0321306D0 (en) 2003-09-11 2003-10-15 Solexa Ltd Modified polymerases for improved incorporation of nucleotide analogues
US7169560B2 (en) * 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
JP2007525571A (ja) 2004-01-07 2007-09-06 ソレクサ リミテッド 修飾分子アレイ
WO2006044078A2 (en) 2004-09-17 2006-04-27 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
EP1828412B2 (en) 2004-12-13 2019-01-09 Illumina Cambridge Limited Improved method of nucleotide detection
WO2006120433A1 (en) 2005-05-10 2006-11-16 Solexa Limited Improved polymerases
GB0514936D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Preparation of templates for nucleic acid sequencing
US7405281B2 (en) 2005-09-29 2008-07-29 Pacific Biosciences Of California, Inc. Fluorescent nucleotide analogs and uses therefor
SG170802A1 (en) 2006-03-31 2011-05-30 Solexa Inc Systems and devices for sequence by synthesis analysis
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
AU2007309504B2 (en) 2006-10-23 2012-09-13 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US8349167B2 (en) 2006-12-14 2013-01-08 Life Technologies Corporation Methods and apparatus for detecting molecular interactions using FET arrays
AU2007334393A1 (en) 2006-12-14 2008-06-26 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
WO2008093098A2 (en) * 2007-02-02 2008-08-07 Illumina Cambridge Limited Methods for indexing samples and sequencing multiple nucleotide templates
EP3431615A3 (en) * 2007-10-19 2019-02-20 The Trustees of Columbia University in the City of New York Dna sequencing with non-fluorescent nucleotide reversible terminators cleavable label modified nucleotide terminators
US20100137143A1 (en) 2008-10-22 2010-06-03 Ion Torrent Systems Incorporated Methods and apparatus for measuring analytes
US20110195410A1 (en) * 2009-12-07 2011-08-11 Gene Check, Inc. Compositions and methods related to solid phase sequence detection and genotyping
CN102140534A (zh) * 2010-12-15 2011-08-03 深圳华大基因科技有限公司 一种hbv基因的核苷酸突变位点的检测方法
US8951781B2 (en) 2011-01-10 2015-02-10 Illumina, Inc. Systems, methods, and apparatuses to image a sample for biological or chemical analysis
CA2830361C (en) * 2011-03-31 2021-03-16 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable multiplex cold-pcr
US20120252682A1 (en) * 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
PT3623481T (pt) 2011-09-23 2021-10-15 Illumina Inc Composições para sequenciação de ácidos nucleicos
IN2014DN07992A (enExample) 2012-04-03 2015-05-01 Illumina Inc
ES2628485T3 (es) * 2013-07-03 2017-08-03 Illumina, Inc. Secuenciación mediante síntesis ortogonal

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5976802A (en) * 1995-04-27 1999-11-02 Europasches Laboratorium fur Molekularbiologie (EMBL) Simultaneous sequencing of nucleic acids

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US20200392573A1 (en) 2020-12-17
AU2021286342B2 (en) 2024-05-30
DK3215638T3 (da) 2021-10-18
CA2966442C (en) 2024-03-12
ES2889875T3 (es) 2022-01-14
EP3215638A1 (en) 2017-09-13
CA2966442A1 (en) 2016-05-12
AU2015341568B2 (en) 2021-09-16
AU2021286342A1 (en) 2022-01-20
US20170298430A1 (en) 2017-10-19
WO2016071689A1 (en) 2016-05-12
GB201419731D0 (en) 2014-12-17
JP2017533709A (ja) 2017-11-16
US11555218B2 (en) 2023-01-17
JP6789935B2 (ja) 2020-11-25
US20230160006A1 (en) 2023-05-25
CN107257862B (zh) 2021-08-03
CN107257862A (zh) 2017-10-17
EP3974538A1 (en) 2022-03-30
SG11201703629XA (en) 2017-06-29
EP3215638B1 (en) 2021-08-11
CN113564238A (zh) 2021-10-29
AU2015341568A1 (en) 2017-05-25
HK1243465A1 (en) 2018-07-13

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