DK3205722T3 - Anordning og fremgangsmåder ved isolering af nukleinsyrer fra blod - Google Patents
Anordning og fremgangsmåder ved isolering af nukleinsyrer fra blod Download PDFInfo
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- DK3205722T3 DK3205722T3 DK16155286.4T DK16155286T DK3205722T3 DK 3205722 T3 DK3205722 T3 DK 3205722T3 DK 16155286 T DK16155286 T DK 16155286T DK 3205722 T3 DK3205722 T3 DK 3205722T3
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- blood
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- composition
- rna
- nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- Engineering & Computer Science (AREA)
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- Biotechnology (AREA)
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- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Tropical Medicine & Parasitology (AREA)
- Mycology (AREA)
- Virology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Claims (9)
- Anordning og fremgangsmåder ved isolering af nukleinsyrer fra blod1. Anvendelsen af en sammensætning som opløsningsmiddel for de celler, der er indeholdt i blodet, som stabiliseringsmiddel for blodets samlede RNA og til isolering ved hjælp af adsorption af RNA ud fra en blanding af blodet med sammensætningen til et adsorptionsmiddel for nukleinsyrer, er kendetegnet ved, at sammensætningen i en vandig opløsning består af a. mindst et guanidinsalt, b. mindst en buffersubstans til afbødning i en pH på 5,0 til 8,0, c. mindst et ikke-ionisk rengøringsmiddel og d. mindst en kompleksdanner til bivalente kationer e. og valgfrit proteinase og/eller DNase og f. er fri for reduktionsmidler, der forhindrer nedbrydningen og resyntetiseringen af RNA i blodet, og at anvendelsen omfatter den umiddelbare isolering af RNA-et fra blandingen, der består af blodet og sammensætningen, hvorved sammensætningen kan planlægges i et variabelt volumenforhold af blod til sammensætning på op til 1:13 i blandingen ved at bringe blodet i kontakt med et adsorptionsmiddel for nukleinsyrer, hvorved der ikke udskilles nogen bestanddel af blandingen, blandingen proteinase og/eller DNase bliver valgfrit tilsat, og hvorved RNA-et ikke falder ud af blandingen, før det bliver bragt i kontakt med adsorptionsmidlet.
- 2. Anvendelsen ifølge krav 1 er kendetegnet ved, at guanidinsaltet er guanidinthiocyanat; at buffersubstansen er2-(A/-morpholino)ethansulfonsyre (MES), tris (tris(hydroxymethyl)-aminometan), citrat, phosphat(di-natriumhydrogenphosphat)dihydrat, bistris-buffer (bis-(2-hydroxymethyl)-imino-tris-(hydroxymethyl)-metan), ACES (N-(2-acetamido)-2-aminomethansulfonsyre), natriumbicarbonat eller phosphat- (natriumhydrogenphosphat)dihydrat; at det ikke-ioniske rengøringsmiddel er Triton X-100, Tween 20, Tween 80 (polyoxyethylensorbitanmonooleat), Brij 58 (polyethylenglycol-hexadecylether), Triton X-114 (octylphenolpolyethylenglycolether) eller Nonidet P40; og at kompleksdanneren er ethylendiamintetraacetat (EDTA), og at den er fri for thiolforbindelser eller disses kemiske prækursorer som reduktionsmidler.
- 3. Anvendelsen ifølge et af de foranstående krav er kendetegnet ved, at sammensætningen er indeholdt i et reagensglas.
- 4. Anvendelsen ifølge krav 3 er kendetegnet ved, at reagensglasset er indrettet til at modtage en variabel mængde blod.
- 5. Fremgangsmåden til isolering af det samlede RNA fra blodet er kendetegnet ved blanding af blodet med en sammensætning, der i en vandig opløsning består af a. mindst et guanidinsalt, b. mindst en buffersubstans, c. mindst et ikke-ionisk rengøringsmiddel og d. mindst en kompleksdanner, e. optionelt proteinase og/eller DNase og er fri for reduktionsmidler, der forhinder opbygningen og syntetiseringer af RNA i blodet; fremstilling af en blanding, der består af blodet og sammensætningen i et volumenforhold på op til 1:13, valgfrit med oplagring af den opnåede blanding ved over 0°C, valgfrit med tilsætning af proteinase og/eller DNase til blandingen; blandingen bringes i kontakt med et adsorptionsmiddel til nukleinsyrer, hvorved der ikke udskilles noget indholdsstof fra blandingen ved kontakten med adsorptionsmidlet; fjernelse af ubundne stoffer fra adsorptionsmidlet og eluering af RNA fra adsorptionmidlet.
- 6. Fremgangsmåden ifølge krav 5 er kendetegnet ved, at blandingens kontakt med adsorptionsmidlet lykkes uden forudgående udfald, udskillelse eller opløsning af nukleinsyrerne fra blandingen.
- 7. Fremgangsmåden ifølge enten krav 5 eller 6 er kendetegnet ved, at det lykkes at oplagre blandingen i mindst 3 dage ved maksimalt 25°C.
- 8. Fremgangsmåden ifølge et af kravene 5 til 7 er kendetegnet ved, at det lykkes at blande blodet med sammensætningen i et volumenforhold på op til 1:2.
- 9. Fremgangsmåden ifølge et af kravene 5 til 8 er kendetegnet ved, at adsorptionsmidlet er kiselbelagte partikler, og at der bliver tilsat en fortynder til blandingen af blod og sammensætning i et volumen på mindst 50 % af blandingens volumen.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP16155286.4A EP3205722B1 (de) | 2016-02-11 | 2016-02-11 | Vorrichtung und verfahren zur isolierung von nukleinsäuren aus vollblut |
Publications (1)
Publication Number | Publication Date |
---|---|
DK3205722T3 true DK3205722T3 (da) | 2018-11-26 |
Family
ID=55409706
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DK16155286.4T DK3205722T3 (da) | 2016-02-11 | 2016-02-11 | Anordning og fremgangsmåder ved isolering af nukleinsyrer fra blod |
Country Status (10)
Country | Link |
---|---|
US (1) | US11118174B2 (da) |
EP (1) | EP3205722B1 (da) |
JP (1) | JP7028800B2 (da) |
CN (1) | CN109312330B (da) |
CA (1) | CA3020959A1 (da) |
DK (1) | DK3205722T3 (da) |
ES (1) | ES2694285T3 (da) |
PL (1) | PL3205722T3 (da) |
RU (1) | RU2731724C2 (da) |
WO (1) | WO2017137573A1 (da) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
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US11473004B2 (en) | 2016-12-02 | 2022-10-18 | University Of Wyoming | Microemulsions and uses thereof to displace oil in heterogeneous porous media |
AU2021314104A1 (en) * | 2020-07-20 | 2023-01-19 | Insmed, Inc. | Methods for extracting neutrophil serine proteases and treating dipeptidyl peptidase 1-mediated conditions |
CN111926055B (zh) * | 2020-09-23 | 2021-02-05 | 北京健为医学检验实验室有限公司 | 一种hpv样品采集保存卡及其制备方法 |
WO2022150283A1 (en) * | 2021-01-05 | 2022-07-14 | Nuclease Probe Technologies, Inc. | Sars-cov-2 detection |
EP4163370A1 (en) | 2021-10-08 | 2023-04-12 | AXAGARIUS GmbH & Co. KG | Stabilization of nucleic acids in biological samples |
Family Cites Families (16)
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NL8900725A (nl) * | 1989-03-23 | 1990-10-16 | Az Univ Amsterdam | Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur. |
DE19836559A1 (de) * | 1998-08-12 | 2000-03-23 | Antigen Gmbh | Gefäß zur Entnahme von Blut |
DE19856064C2 (de) * | 1998-12-04 | 2000-11-30 | Invitek Gmbh | Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien |
US7100246B1 (en) * | 1999-06-14 | 2006-09-05 | E. I. Du Pont De Nemours And Company | Stretch break method and product |
CA2428864C (en) | 2000-11-08 | 2011-04-12 | Becton, Dickinson And Company | Method and device for collecting and stabilizing a biological sample |
DE10147439B4 (de) * | 2001-09-26 | 2014-01-30 | Qiagen Gmbh | Verfahren zur Isolierung von DNA aus biologischen Proben |
WO2003068918A2 (en) * | 2002-02-11 | 2003-08-21 | Auburn University | High-sensitivity real-time polymerase chain reaction for detection of nucleic acids |
KR20130143677A (ko) * | 2004-11-05 | 2013-12-31 | 퀴아젠 노쓰 아메리칸 홀딩즈, 인크. | 안정화 시약으로부터 핵산을 정제하기 위한 조성물 및 방법 |
DE102005057334A1 (de) * | 2005-11-28 | 2007-06-06 | Aj Innuscreen Gmbh | Verfahren zur Isolierung von Nukleinsäuren aus beliebigen Ausgangsmaterialien |
CA2694411A1 (en) * | 2007-07-27 | 2009-02-05 | Ge Healthcare Bio-Sciences Corp. | An improved nucleic acid purification method |
WO2014146781A1 (en) * | 2013-03-18 | 2014-09-25 | Qiagen Gmbh | Stabilisation of biological samples |
CN103820431B (zh) * | 2014-02-25 | 2016-10-05 | 苏州天隆生物科技有限公司 | 基于纳米磁珠的核酸提取纯化方法及试剂盒 |
US9896683B2 (en) * | 2014-07-30 | 2018-02-20 | University Of Massachusetts | Isolating circulating microRNA (miRNA) |
DE102014220090B3 (de) * | 2014-10-02 | 2015-10-08 | Abf Diagnostics Gmbh | Probennahmevorrichtung mit Probenaufarbeitungsfunktion |
CN104673623B (zh) * | 2015-02-05 | 2018-03-09 | 广州维帝医疗技术有限公司 | 血液样品收集装置 |
EP3389063A1 (en) | 2017-04-13 | 2018-10-17 | Comet AG | Variable vacuum capacitor and cooling method |
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2016
- 2016-02-11 ES ES16155286.4T patent/ES2694285T3/es active Active
- 2016-02-11 PL PL16155286T patent/PL3205722T3/pl unknown
- 2016-02-11 DK DK16155286.4T patent/DK3205722T3/da active
- 2016-02-11 EP EP16155286.4A patent/EP3205722B1/de active Active
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2017
- 2017-02-10 JP JP2018561307A patent/JP7028800B2/ja active Active
- 2017-02-10 CN CN201780022820.2A patent/CN109312330B/zh active Active
- 2017-02-10 CA CA3020959A patent/CA3020959A1/en active Pending
- 2017-02-10 WO PCT/EP2017/053025 patent/WO2017137573A1/de active Application Filing
- 2017-02-10 RU RU2018132166A patent/RU2731724C2/ru active
- 2017-02-10 US US16/077,123 patent/US11118174B2/en active Active
Also Published As
Publication number | Publication date |
---|---|
WO2017137573A1 (de) | 2017-08-17 |
EP3205722A1 (de) | 2017-08-16 |
BR112018016394A2 (pt) | 2018-12-18 |
EP3205722B1 (de) | 2018-08-01 |
US11118174B2 (en) | 2021-09-14 |
JP2019509058A (ja) | 2019-04-04 |
CN109312330A (zh) | 2019-02-05 |
RU2018132166A3 (da) | 2020-03-11 |
JP7028800B2 (ja) | 2022-03-02 |
ES2694285T3 (es) | 2018-12-19 |
RU2731724C2 (ru) | 2020-09-08 |
US20190127729A1 (en) | 2019-05-02 |
PL3205722T3 (pl) | 2019-05-31 |
RU2018132166A (ru) | 2020-03-11 |
CA3020959A1 (en) | 2017-08-17 |
CN109312330B (zh) | 2022-08-23 |
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