DK2574662T3 - Fremgangsmåde til elektroporation af biologiske prøver - Google Patents

Fremgangsmåde til elektroporation af biologiske prøver Download PDF

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Publication number
DK2574662T3
DK2574662T3 DK12171932.2T DK12171932T DK2574662T3 DK 2574662 T3 DK2574662 T3 DK 2574662T3 DK 12171932 T DK12171932 T DK 12171932T DK 2574662 T3 DK2574662 T3 DK 2574662T3
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DK
Denmark
Prior art keywords
electroporation
biological samples
biological
samples
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DK12171932.2T
Other languages
English (en)
Inventor
Sergey M Dzekunov
Linhong Li
Vininder Singh
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Maxcyte Inc
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Publication of DK2574662T3 publication Critical patent/DK2574662T3/da

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/02—Details
    • A61N1/04—Electrodes
    • A61N1/0404—Electrodes for external use
    • A61N1/0408—Use-related aspects
    • A61N1/0412—Specially adapted for transcutaneous electroporation, e.g. including drug reservoirs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0091—Purification or manufacturing processes for gene therapy compositions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
    • A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
    • A61K9/5005—Wall or coating material
    • A61K9/5063—Compounds of unknown constitution, e.g. material from plants or animals
    • A61K9/5068—Cell membranes or bacterial membranes enclosing drugs
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/18—Applying electric currents by contact electrodes
    • A61N1/32—Applying electric currents by contact electrodes alternating or intermittent currents
    • A61N1/327—Applying electric currents by contact electrodes alternating or intermittent currents for enhancing the absorption properties of tissue, e.g. by electroporation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2740/00—Reverse transcribing RNA viruses
    • C12N2740/00011—Details
    • C12N2740/10011—Retroviridae
    • C12N2740/10051—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Radiology & Medical Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Epidemiology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Manufacturing & Machinery (AREA)
  • Sustainable Development (AREA)
  • Virology (AREA)
  • Electromagnetism (AREA)
  • Botany (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
DK12171932.2T 2001-08-22 2002-08-21 Fremgangsmåde til elektroporation af biologiske prøver DK2574662T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US31424101P 2001-08-22 2001-08-22
US35457102P 2002-02-05 2002-02-05
EP02761450.2A EP1456345B1 (en) 2001-08-22 2002-08-21 Apparatus and method for electroporation of biological samples

Publications (1)

Publication Number Publication Date
DK2574662T3 true DK2574662T3 (da) 2021-09-20

Family

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Family Applications (2)

Application Number Title Priority Date Filing Date
DK12171932.2T DK2574662T3 (da) 2001-08-22 2002-08-21 Fremgangsmåde til elektroporation af biologiske prøver
DK02761450.2T DK1456345T3 (da) 2001-08-22 2002-08-21 Apparat og fremgangsmåde til elektroporering af biologiske prøver

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK02761450.2T DK1456345T3 (da) 2001-08-22 2002-08-21 Apparat og fremgangsmåde til elektroporering af biologiske prøver

Country Status (6)

Country Link
US (3) US7141425B2 (da)
EP (2) EP1456345B1 (da)
AU (1) AU2002326717A1 (da)
CA (1) CA2459697C (da)
DK (2) DK2574662T3 (da)
WO (1) WO2003018751A2 (da)

Families Citing this family (104)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6773669B1 (en) * 1995-03-10 2004-08-10 Maxcyte, Inc. Flow electroporation chamber and method
US7029916B2 (en) * 2001-02-21 2006-04-18 Maxcyte, Inc. Apparatus and method for flow electroporation of biological samples
US20080172026A1 (en) 2006-10-17 2008-07-17 Blomquist Michael L Insulin pump having a suspension bolus
EP1553176A4 (en) 2002-10-16 2008-10-01 Universal Bio Research Co Ltd DEVICE FOR INTRODUCING BIOLOGICAL MATERIAL, METHOD FOR INTRODUCING BIOLOGICAL MATERIAL AND MAGNETIC SUPPORT FOR INTRODUCING BIOLOGICAL MATERIAL
JP2006518219A (ja) * 2003-02-18 2006-08-10 マックスサイト インコーポレーティッド 電気穿孔法による細胞への抗原の負荷方法
US20060134067A1 (en) * 2003-02-18 2006-06-22 Maxcyte, Inc. Loading of cells with antigens by electroporation
US7015615B2 (en) * 2003-03-17 2006-03-21 Virginia Tech Intellectual Properties, Inc. Apparatus and method that prevent flux reversal in the stator back material of a two-phase SRM (TPSRM)
US20050059999A1 (en) * 2003-09-15 2005-03-17 Mongeon Luc R. Delivering genetic material to a stimulation site
US7521224B2 (en) * 2003-09-30 2009-04-21 The United States Of America As Represented By The Secretary Of The Navy Microelectronic cell electroporation array
DE102004013762B4 (de) * 2004-03-20 2006-08-03 Forschungszentrum Karlsruhe Gmbh Verfahren zur besseren und schonenden Freisetzung wertgebender Inhaltsstoffe aus Weinbeeren und ein daraus gewonnener Most
AU2011211362B2 (en) * 2004-05-12 2014-05-29 Maxcyte, Inc. Methods and devices related to a regulated flow electroporation chamber
AU2005245871A1 (en) * 2004-05-12 2005-12-01 Maxcyte, Inc. Methods and devices related to a regulated flow electroporation chamber
US20060165668A1 (en) * 2004-12-10 2006-07-27 Liu Linda N Genetically modified tumor cells as cancer vaccines
WO2006089152A2 (en) * 2005-02-18 2006-08-24 Maxcyte, Inc. Use of methyltransferase inhibitors to enhance transgene expression
WO2006102684A2 (en) 2005-03-19 2006-09-28 The Regents Of The University Of California Ultra low strength electric field network-mediated ex vivo gene, protein and drug delivery in cells
WO2007021994A2 (en) 2005-08-12 2007-02-22 Maxcyte, Inc. Electrode shielding for electroporation
US20070059833A1 (en) * 2005-09-07 2007-03-15 Maxcyte, Inc. Use of Nucleases to Improve Viability and Enhance Transgene Expression in Transfected Cells
WO2007053802A2 (en) * 2005-10-20 2007-05-10 The Ohio State University Drug and gene delivery by polymer nanonozzle and nanotip cell patch
KR20080086983A (ko) * 2005-12-07 2008-09-29 제네트로닉스, 인코포레이티드 가변 부피의 전기천공 챔버 및 이의 사용 방법
CN101432425A (zh) * 2006-01-04 2009-05-13 环球健康有限公司 通过转染由成体干细胞产生胰岛素分泌细胞的方法
EA200870252A1 (ru) * 2006-02-11 2009-02-27 Дженетроникс, Инк. Устройство и способ одноигольного электропорообразования in vivo
US7615385B2 (en) 2006-09-20 2009-11-10 Hypres, Inc Double-masking technique for increasing fabrication yield in superconducting electronics
US20090004742A1 (en) * 2006-11-01 2009-01-01 Duke University Selection of antigen-specific t cells
WO2009029613A1 (en) * 2007-08-31 2009-03-05 The Board Of Regents, The University Of Texas System Apparatus for performing magnetic electroporation
US8093038B2 (en) * 2007-09-17 2012-01-10 Illinois Institute Of Technology Apparatus and method for encapsulating pancreatic cells
US8986253B2 (en) 2008-01-25 2015-03-24 Tandem Diabetes Care, Inc. Two chamber pumps and related methods
WO2009123564A1 (en) * 2008-04-03 2009-10-08 Nanyang Polytechnic Apparatus for electroporation with cooling system
JP5779090B2 (ja) 2008-04-09 2015-09-16 マックスサイト インコーポレーティッド 新規に単離された細胞の治療組成物の操作および送達
US20090295461A1 (en) * 2008-05-30 2009-12-03 Cesare Joshua De Device configuration
US8221411B2 (en) * 2008-07-28 2012-07-17 Medtronic, Inc. Systems and methods for cardiac tissue electroporation ablation
US8408421B2 (en) 2008-09-16 2013-04-02 Tandem Diabetes Care, Inc. Flow regulating stopcocks and related methods
US8650937B2 (en) 2008-09-19 2014-02-18 Tandem Diabetes Care, Inc. Solute concentration measurement device and related methods
AU2010278894B2 (en) 2009-07-30 2014-01-30 Tandem Diabetes Care, Inc. Infusion pump system with disposable cartridge having pressure venting and pressure feedback
WO2011032149A2 (en) * 2009-09-14 2011-03-17 Board Of Regents, The University Of Texas System Bipolar solid state marx generator
US8810236B2 (en) * 2010-03-09 2014-08-19 Nokia Corporation Apparatus and associated methods
WO2011127129A1 (en) 2010-04-06 2011-10-13 Holaday John W Methods of treating cancer
JP2013527009A (ja) * 2010-06-03 2013-06-27 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア エレクトロポレーション電極構造および方法
US9078878B2 (en) 2010-12-01 2015-07-14 Alderbio Holdings Llc Anti-NGF antibodies that selectively inhibit the association of NGF with TrkA, without affecting the association of NGF with p75
US9539324B2 (en) 2010-12-01 2017-01-10 Alderbio Holdings, Llc Methods of preventing inflammation and treating pain using anti-NGF compositions
US9067988B2 (en) 2010-12-01 2015-06-30 Alderbio Holdings Llc Methods of preventing or treating pain using anti-NGF antibodies
CN103619879A (zh) 2010-12-01 2014-03-05 奥尔德生物控股有限责任公司 抗ngf组合物及其用途
US11214610B2 (en) 2010-12-01 2022-01-04 H. Lundbeck A/S High-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris
US9884909B2 (en) 2010-12-01 2018-02-06 Alderbio Holdings Llc Anti-NGF compositions and use thereof
US9592398B2 (en) 2011-05-12 2017-03-14 Medtronic, Inc. Leadless implantable medical device with osmotic pump
US20130344047A1 (en) * 2011-12-21 2013-12-26 Abbott Cardiovascular Systems, Inc. Methods And Composition For Treating Heart Failure And Ischemia
US9335910B2 (en) 2012-04-23 2016-05-10 Tandem Diabetes Care, Inc. System and method for reduction of inadvertent activation of medical device during manipulation
US9180242B2 (en) 2012-05-17 2015-11-10 Tandem Diabetes Care, Inc. Methods and devices for multiple fluid transfer
US9555186B2 (en) 2012-06-05 2017-01-31 Tandem Diabetes Care, Inc. Infusion pump system with disposable cartridge having pressure venting and pressure feedback
US9715327B2 (en) 2012-06-07 2017-07-25 Tandem Diabetes Care, Inc. Preventing inadvertent changes in ambulatory medical devices
US9381297B2 (en) 2012-06-07 2016-07-05 Tandem Diabetes Care, Inc. Sealed infusion device with electrical connector port
US9173998B2 (en) 2013-03-14 2015-11-03 Tandem Diabetes Care, Inc. System and method for detecting occlusions in an infusion pump
US9044611B2 (en) * 2013-03-15 2015-06-02 The Regents Of The University Of California Systems and methods for selectively migrating cells using electric fields
US10316335B2 (en) 2013-03-15 2019-06-11 Maxcyte, Inc. Methods and compositions for generating stable transfected cells
US9078862B2 (en) 2013-06-06 2015-07-14 General Electric Company Platelet activation using long electric field pulses
US9238808B2 (en) 2013-06-06 2016-01-19 General Electric Company Modular adjustable pulse generator
CN103555574A (zh) * 2013-11-11 2014-02-05 苏州文曲生物微系统有限公司 一种流式电穿孔装置
KR102406585B1 (ko) * 2014-04-14 2022-06-07 맥스시티 인코포레이티드 게놈 dna를 변형하기 위한 방법 및 조성물
EP3260163B1 (en) * 2014-05-02 2019-07-17 Lonza Cologne GmbH Device and method for large volume transfection
CN107429254B (zh) 2015-01-30 2021-10-15 加利福尼亚大学董事会 原代造血细胞中的蛋白递送
US11608511B2 (en) 2015-04-13 2023-03-21 Maxcyte, Inc. Methods for modifying genomic DNA
CA3019814A1 (en) 2016-04-04 2017-10-12 CyteQuest, Inc. System, device and method for electroporation of cells
JP2019520844A (ja) 2016-07-21 2019-07-25 マックスサイト インコーポレーティッド ゲノムｄｎａを改変するための方法および組成物
US10724043B2 (en) * 2016-10-06 2020-07-28 Nantbio, Inc. Multi-pulse transfection methods and cells
HRP20220615T1 (hr) 2017-06-30 2022-06-24 Inscripta, Inc. Postupci, moduli, instrumenti i sustavi za automatiziranu obradu stanica
US10738327B2 (en) 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
US10443074B2 (en) 2017-09-30 2019-10-15 Inscripta, Inc. Modification of cells by introduction of exogenous material
EP3775159A4 (en) 2018-03-29 2022-01-19 Inscripta, Inc. AUTOMATED REGULATION OF CELL GROWTH RATES FOR INDUCTION AND TRANSFORMATION
WO2019200004A1 (en) 2018-04-13 2019-10-17 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
WO2019209926A1 (en) 2018-04-24 2019-10-31 Inscripta, Inc. Automated instrumentation for production of peptide libraries
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
US10526598B2 (en) 2018-04-24 2020-01-07 Inscripta, Inc. Methods for identifying T-cell receptor antigens
EP3790607B1 (en) 2018-05-11 2023-12-27 Lupagen, Inc. Systems for closed loop, real-time modifications of patient cells
US11377652B2 (en) 2018-07-09 2022-07-05 NanoCav, LLC Micro flow-through electroporation devices and methods of cell transfection
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
IL292273B2 (en) 2018-08-14 2023-10-01 Inscripta Inc Devices, modules and methods for improved detection of edited sequences in living cells
US10752874B2 (en) 2018-08-14 2020-08-25 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
CA3108892A1 (en) 2018-08-30 2020-04-23 Inscripta, Inc. Improved detection of nuclease edited sequences in automated modules and instruments
CA3111720A1 (en) * 2018-08-31 2020-03-05 The Charles Stark Draper Laboratory, Inc. Method and apparatus for high throughput high efficiency transfection of cells
BR112021007913A2 (pt) * 2018-10-26 2021-08-03 Kytopen Corporation dispositivos, sistemas e kits para eletroporação e métodos de uso dos mesmos
KR102232757B1 (ko) * 2018-11-22 2021-03-26 (주)엑솔런스바이오테크놀로지 체외충격파를 이용한 표적물질 전달 장치
EP3886879A4 (en) 2018-11-30 2022-12-07 Cellphire Inc. PLATES AS DELIVERY AGENTS
CA3121200A1 (en) 2018-11-30 2020-06-04 Cellphire, Inc. Platelets as delivery agents
CN114072159A (zh) 2019-05-03 2022-02-18 塞尔菲乐有限公司 用于生产血液产品的材料和方法
US10907125B2 (en) 2019-06-20 2021-02-02 Inscripta, Inc. Flow through electroporation modules and instrumentation
CN114008070A (zh) 2019-06-21 2022-02-01 因思科瑞普特公司 导致大肠杆菌赖氨酸产量增加的全基因组合理设计的突变
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
WO2021003451A1 (en) * 2019-07-02 2021-01-07 Kytopen Corporation Devices, systems, and kits for electro-mechanical delivery and methods of use thereof
US20210299179A1 (en) 2020-02-04 2021-09-30 Cellphire, Inc. Methods of treating congenital hemophilia with anti-fibrinolytic loaded platelets
BR112022002892A2 (pt) 2019-08-16 2022-05-24 Cellphire Inc Trombossomos como um agente de reversão de agente antiplaqueta
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
WO2021154706A1 (en) 2020-01-27 2021-08-05 Inscripta, Inc. Electroporation modules and instrumentation
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
USD943760S1 (en) * 2020-05-11 2022-02-15 Maxcyte, Inc. Electroporation processing assembly
USD944410S1 (en) * 2020-05-11 2022-02-22 Maxcyte, Inc. Electroporation processing assembly
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
USD1016324S1 (en) 2020-07-08 2024-02-27 NanoCav, LLC Biological cell processing chip
USD965170S1 (en) 2020-10-23 2022-09-27 Life Technologies Corporation Electroporation device
IL311657A (en) 2021-09-24 2024-05-01 Maxcyte Inc A method of improved viral transduction using electroporation
WO2023081424A2 (en) * 2021-11-05 2023-05-11 Spot Biosystems Ltd. Centrifugal cell positioning system and methods
WO2023158755A1 (en) 2022-02-16 2023-08-24 Maxcyte, Inc. A method of cell electroporation
EP4279085A1 (en) 2022-05-20 2023-11-22 Mnemo Therapeutics Compositions and methods for treating a refractory or relapsed cancer or a chronic infectious disease
WO2024059618A2 (en) 2022-09-13 2024-03-21 Arsenal Biosciences, Inc. Immune cells having co-expressed tgfbr shrnas
WO2024059824A2 (en) 2022-09-16 2024-03-21 Arsenal Biosciences, Inc. Immune cells with combination gene perturbations

Family Cites Families (85)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2955076A (en) 1955-10-05 1960-10-04 Gen Electric Co Ltd Artificial mutation of micro-organisms by electrical shock
GB1304518A (da) 1969-06-27 1973-01-24
GB1481480A (en) 1974-02-02 1977-07-27 Kernforschungsanlage Juelich Process and apparatus for increasing the permeability of the membrane of cells of organisms
US4075076A (en) 1974-06-24 1978-02-21 Kurt Xylander Water treatment apparatus
GB1595183A (en) 1977-03-04 1981-08-12 Ici Ltd Diaphragm cell
US4192869A (en) 1977-09-06 1980-03-11 Studiengesellschaft Kohle Mbh. Controlled improvement of the O2 release by intact erythrocytes with lipid vesicles
DE2740053A1 (de) 1977-09-06 1979-05-03 Klaus Prof Dr Med Gersonde Verwendung von allosterischen effektoren mit hilfe von lipidvesikeln ueber eine irreversible inkorporierung zwecks verbesserter o tief 2 -entladung des haemoglobins in erythrozyten
DE3108195C2 (de) 1981-03-04 1984-07-12 Windmöller & Hölscher, 4540 Lengerich Vorrichtung zum Bilden von verpackungsfähigen Stapeln aus flachen Werkstücken
US4849355A (en) 1985-01-08 1989-07-18 Wong Tai Kin Method of transferring genes into cells
FR2529463B1 (fr) 1982-07-05 1986-01-10 Centre Nat Rech Scient Procede et dispositif pour l'encapsulation dans les erythrocytes d'au moins une substance a activite biologique, notamment des effecteurs allosteriques de l'hemoglobine et erythrocytes ainsi obtenus
US4476004A (en) 1983-04-08 1984-10-09 D.E.P. Systems, Inc. Apparatus for electrofusion of biological particles
DE3317415A1 (de) 1983-05-13 1984-11-15 Kernforschungsanlage Jülich GmbH, 5170 Jülich Kammer zur behandlung von zellen im elektrischen feld
US4478824A (en) 1983-08-08 1984-10-23 Franco Robert S Method for altering red blood cell function and survival
DE3483934D1 (de) 1983-10-13 1991-02-21 Rikagaku Kenkyusho Verfahren und apparat zum einpflanzen eines fremdstoffes in lebende zelle.
US4622302A (en) 1984-08-09 1986-11-11 American National Red Cross Process for inducing membrane fusion under an electric field
US5100792A (en) 1984-11-13 1992-03-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues
US5036006A (en) 1984-11-13 1991-07-30 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US4663292A (en) 1984-12-21 1987-05-05 Wong Daniel T High-voltage biological macromolecule transfer and cell fusion system
DE3522610A1 (de) 1985-06-12 1986-12-18 Kernforschungsanlage Jülich GmbH, 5170 Jülich Kammer fuer die behandlung von zellen im elektrischen feld
KR890003947B1 (ko) 1985-12-11 1989-10-13 가부시기가이샤 시마즈세이사구쇼 세포융합장치
JPS62151174A (ja) 1985-12-24 1987-07-06 Akira Mizuno 高電圧パルスによる細胞破壊装置
JPH0655143B2 (ja) 1985-12-28 1994-07-27 株式会社島津製作所 遺伝子等の導入装置
DE3603029A1 (de) 1986-01-31 1987-08-06 Interatom Korrosionsschutzelektrode mit ueberzug aus titanverbindung
JPS62228277A (ja) 1986-03-27 1987-10-07 Kirin Brewery Co Ltd エレクトロポレ−シヨン
US4882281A (en) 1986-04-02 1989-11-21 Jeffrey L. Hilliard Probe for electrofusion, electroporation, or like procedure
US4695547A (en) 1986-04-02 1987-09-22 Jeffrey L. Hilliard Probe for electrofusion, electroporation, or like procedure
US4784737A (en) 1986-04-18 1988-11-15 The United States Department Of Energy Electromicroinjection of particles into living cells
US4946793A (en) 1986-05-09 1990-08-07 Electropore, Inc. Impedance matching for instrumentation which electrically alters vesicle membranes
US4849089A (en) 1986-05-09 1989-07-18 Electropore, Inc. Disposable electromanipulation chamber
US4906576A (en) 1986-05-09 1990-03-06 Electropore, Inc. High speed, high power apparatus for vesicle prealignment, poration, loading and fusion in uniform electric fields and method therefor
US4923814A (en) 1986-05-09 1990-05-08 Electropore, Inc. High speed, high power apparatus for vesicle prealignment, poration, loading and fusion in uniform electric fields and method therefor
JPS62265975A (ja) 1986-05-12 1987-11-18 Shimadzu Corp 細胞への高分子導入装置
JPH0714345B2 (ja) 1986-12-03 1995-02-22 株式会社日立製作所 生試料のレ−ザ加工方法
WO1988004322A1 (en) 1986-12-07 1988-06-16 Biscar Jean P Artificial gravity cellular molecular injection
US4800163A (en) 1986-12-15 1989-01-24 Ntl. Inst. of Agrobiological Resources Flow chamber and electro-manipulator incorporating same
DE3715170C2 (de) 1987-05-07 1994-04-28 Andreas Prof Dr Ziegler Vorrichtung und Verfahren zur elektrophoretischen Trennung von Makromolekülen sowie die Verwendung des Verfahrens
US4840714A (en) 1987-05-13 1989-06-20 American Bionetics, Inc. Electroblotting technique for transferring specimens from a polyacrylamide electrophoresis or like gel onto a membrane
US5098843A (en) 1987-06-04 1992-03-24 Calvin Noel M Apparatus for the high efficiency transformation of living cells
US5128257A (en) 1987-08-31 1992-07-07 Baer Bradford W Electroporation apparatus and process
GB8721015D0 (en) 1987-09-07 1987-10-14 Amersham Int Plc Modifying living cells
US4822470A (en) 1987-10-09 1989-04-18 Baylor College Of Medicine Method of and apparatus for cell poration and cell fusion using radiofrequency electrical pulses
US4970154A (en) 1987-10-09 1990-11-13 Baylor College Of Medicine Method for inserting foreign genes into cells using pulsed radiofrequency
EP0386086B1 (en) 1987-10-09 1996-05-29 Baylor College Of Medicine Method for cell poration and cell fusion using radiofrequency electrical pulses
US4931276A (en) 1987-10-30 1990-06-05 Franco Robert S Method for introducing desired agents into red blood cells
JPH01141582A (ja) 1987-11-27 1989-06-02 Shimadzu Corp 遺伝子導入装置
US4910140A (en) 1988-04-18 1990-03-20 Bio-Rad Laboratories, Inc. Electroporation of prokaryotic cells
US4956288A (en) 1988-04-22 1990-09-11 Biogen, Inc. Method for producing cells containing stably integrated foreign DNA at a high copy number, the cells produced by this method, and the use of these cells to produce the polypeptides coded for by the foreign DNA
US5043261A (en) 1989-04-10 1991-08-27 Cryopharm Corporation Lyophilized and reconstituted red blood cell and hemosome compositions
US4874690A (en) 1988-08-26 1989-10-17 Cryopharm Corporation Lyophilization of red blood cells
CA1327174C (en) 1988-10-05 1994-02-22 Youssef Mouneimne Electro-insertion of proteins into animal cell membranes
JPH0687783B2 (ja) 1988-11-10 1994-11-09 浜松ホトニクス株式会社 細胞電気穿孔法および装置
JPH0687782B2 (ja) 1988-11-10 1994-11-09 浜松ホトニクス株式会社 細胞電気穿孔法およびその装置
JPH02186993A (ja) 1989-01-13 1990-07-23 Hitachi Ltd 生細胞へのレーザー光照射方法
US5007995A (en) 1989-05-11 1991-04-16 Olympus Optical Co., Ltd. Device for electrofusion of cells
DE3925680A1 (de) 1989-08-03 1991-02-07 Kruess Gmbh Wissenschaftliche Verfahren zur herstellung vitaler wirkstoff-beladener humanerythrozyten
US5124259A (en) 1989-08-23 1992-06-23 Mitsui Toatsu Chemicals, Inc. Method for electroporation
US5100627A (en) 1989-11-30 1992-03-31 The Regents Of The University Of California Chamber for the optical manipulation of microscopic particles
JPH03195485A (ja) 1989-12-25 1991-08-27 Kazuo Yanagi エレクトロポレーション装置
US5114681A (en) 1990-03-09 1992-05-19 Biomedical Research And Development Laboratories, Inc. Superfusion apparatus
BE1004328A3 (fr) 1990-05-16 1992-11-03 Scient Equipment Design & Dev Procede et dispositif de permeabilisation de cellules vivantes.
JPH0427393A (ja) 1990-05-22 1992-01-30 Iseki & Co Ltd 植物細胞への遺伝子導入法
US5134070A (en) 1990-06-04 1992-07-28 Casnig Dael R Method and device for cell cultivation on electrodes
US5135667A (en) 1990-06-14 1992-08-04 Baxter International Inc. Method and apparatus for administration of anticoagulant to red cell suspension output of a blood separator
CA2019758C (en) 1990-06-25 2001-09-04 Kevin L. Firth Improved electroporation device and method
US5139684A (en) 1990-08-06 1992-08-18 Steven Kaali Electrically conductive methods and systems for treatment of blood and other body fluids and/or synthetic fluids with electric forces
US5137817A (en) 1990-10-05 1992-08-11 Amoco Corporation Apparatus and method for electroporation
US5545130A (en) 1992-04-08 1996-08-13 Genetronics, Inc. Flow through electroporation method
US5501662A (en) 1992-05-22 1996-03-26 Genetronics, Inc. Implantable electroporation method and apparatus for drug and gene delivery
US5424209A (en) 1993-03-19 1995-06-13 Kearney; George P. Automated cell culture and testing system
US5612207A (en) 1993-03-23 1997-03-18 Cbr Laboratories, Inc. Method and apparatus for encapsulation of biologically-active substances in cells
JP3012430B2 (ja) 1993-06-07 2000-02-21 富士写真フイルム株式会社 光ディスクの再生復旧システム
US6506604B2 (en) 1993-06-11 2003-01-14 Cell Genesys, Inc. Method for production of high titer virus and high efficiency retroviral mediated transduction of mammalian cells
JPH07180029A (ja) 1993-12-24 1995-07-18 Kobe Steel Ltd 耐食性材料及びその製造方法
JP3237394B2 (ja) 1994-05-24 2001-12-10 松下電器産業株式会社 電気化学素子
DE4440386A1 (de) 1994-11-11 1996-05-15 Pacesetter Ab Elektroden für medizinische Anwendungen
US6074605A (en) 1995-03-10 2000-06-13 Entremed, Inc. Flow electroporation chamber and method
US5720921A (en) 1995-03-10 1998-02-24 Entremed, Inc. Flow electroporation chamber and method
CN1195997A (zh) 1995-03-10 1998-10-14 恩特雷麦德有限公司 流式电穿孔室和方法
SE9504233D0 (sv) 1995-11-27 1995-11-27 Pacesetter Ab Implantable medical device
ATE229968T1 (de) 1996-03-28 2003-01-15 Nihon Mediphysics Co Ltd Diagnostisches mittel zur glykostoffwechselfunktion
US6090617A (en) * 1996-12-05 2000-07-18 Entremed, Inc. Flow electroporation chamber with electrodes having a crystalline metal nitride coating
US20010001064A1 (en) 1998-03-18 2001-05-10 Holaday John W. Flow electroporation chamber and methods of use thereof
WO2001024830A2 (en) 1999-10-05 2001-04-12 Theramed, Inc. Incorporation of chemical substances into cells
WO2001081553A1 (en) 2000-04-25 2001-11-01 Chiron Corporation Alphavirus-based vectors for persistent infection

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US7186559B2 (en) 2007-03-06
US20030073238A1 (en) 2003-04-17
DK1456345T3 (da) 2016-09-26
AU2002326717A1 (en) 2003-03-10
US20080138877A1 (en) 2008-06-12
US20040197883A1 (en) 2004-10-07
WO2003018751A3 (en) 2004-07-15
CA2459697A1 (en) 2003-03-06
EP2574662B1 (en) 2021-08-04
CA2459697C (en) 2016-04-12
EP1456345A4 (en) 2009-06-24
EP1456345A2 (en) 2004-09-15
EP2574662A2 (en) 2013-04-03
US7141425B2 (en) 2006-11-28
WO2003018751A2 (en) 2003-03-06
EP1456345B1 (en) 2016-07-20

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