DE3483934D1 - Verfahren und apparat zum einpflanzen eines fremdstoffes in lebende zelle. - Google Patents

Verfahren und apparat zum einpflanzen eines fremdstoffes in lebende zelle.

Info

Publication number
DE3483934D1
DE3483934D1 DE8484112218T DE3483934T DE3483934D1 DE 3483934 D1 DE3483934 D1 DE 3483934D1 DE 8484112218 T DE8484112218 T DE 8484112218T DE 3483934 T DE3483934 T DE 3483934T DE 3483934 D1 DE3483934 D1 DE 3483934D1
Authority
DE
Germany
Prior art keywords
planting
foreign substance
living cell
living
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE8484112218T
Other languages
English (en)
Inventor
Takahiro C O Rikagaku K Kasuya
Yoji C O Rikagaku Kenkyu Ikawa
Motoo C O Rikagaku Tsukakoshi
Shunichi Kurata
Yoshio C O Rikagaku Ken Nomiya
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
RIKEN Institute of Physical and Chemical Research
Original Assignee
RIKEN Institute of Physical and Chemical Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP58191379A external-priority patent/JPS6083584A/ja
Priority claimed from JP58191378A external-priority patent/JPS6083583A/ja
Application filed by RIKEN Institute of Physical and Chemical Research filed Critical RIKEN Institute of Physical and Chemical Research
Application granted granted Critical
Publication of DE3483934D1 publication Critical patent/DE3483934D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • H—ELECTRICITY
    • H01—ELECTRIC ELEMENTS
    • H01S—DEVICES USING THE PROCESS OF LIGHT AMPLIFICATION BY STIMULATED EMISSION OF RADIATION [LASER] TO AMPLIFY OR GENERATE LIGHT; DEVICES USING STIMULATED EMISSION OF ELECTROMAGNETIC RADIATION IN WAVE RANGES OTHER THAN OPTICAL
    • H01S3/00—Lasers, i.e. devices using stimulated emission of electromagnetic radiation in the infrared, visible or ultraviolet wave range
    • H01S3/0007—Applications not otherwise provided for
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/005—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor after treatment of microbial biomass not covered by C12N1/02 - C12N1/08
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation

Landscapes

  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Electromagnetism (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plasma & Fusion (AREA)
  • Sustainable Development (AREA)
  • Combustion & Propulsion (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Optics & Photonics (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
DE8484112218T 1983-10-13 1984-10-11 Verfahren und apparat zum einpflanzen eines fremdstoffes in lebende zelle. Expired - Lifetime DE3483934D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP58191379A JPS6083584A (ja) 1983-10-13 1983-10-13 生細胞内への物質移入法
JP58191378A JPS6083583A (ja) 1983-10-13 1983-10-13 生細胞レ−ザ−穿孔装置

Publications (1)

Publication Number Publication Date
DE3483934D1 true DE3483934D1 (de) 1991-02-21

Family

ID=26506660

Family Applications (1)

Application Number Title Priority Date Filing Date
DE8484112218T Expired - Lifetime DE3483934D1 (de) 1983-10-13 1984-10-11 Verfahren und apparat zum einpflanzen eines fremdstoffes in lebende zelle.

Country Status (3)

Country Link
EP (1) EP0137504B1 (de)
CA (1) CA1284302C (de)
DE (1) DE3483934D1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6251990A (ja) * 1985-08-29 1987-03-06 Nippon Zeon Co Ltd 微生物菌体の処理方法
DE3623194A1 (de) * 1986-07-10 1988-03-24 Basf Ag Verfahren zur fusion biologischer zellen und protoplasten mit einem laser-mikrostrahl
DE3889749T2 (de) * 1987-03-06 1994-09-08 Mitsui Petrochemical Ind Verfahren zum Vervielfältigen von Pflanzensämlingen.
FI80185C (fi) * 1987-03-26 1990-05-10 Kemira Oy Foerfarande foer att skaera vaextmaterial.
GB8806643D0 (en) * 1988-03-21 1988-04-20 Ici Plc Genetic manipulation
NZ234283A (en) * 1989-06-30 1993-10-26 Dowelanco Gas ejection of non cellular biological material into cells
CA2031716C (en) * 1989-12-07 1996-06-18 Hiroaki Misawa Laser microprocessing and the device therefor
IT1244667B (it) * 1991-03-28 1994-08-08 Enea Procedimento di microperforazione laser di cellule.
DE69433933T2 (de) * 1993-03-23 2005-07-21 CBR Laboratories, Inc., Boston Verfahren und vorrichtung zur einkapselung biologisch aktiver substanzen in zellen
US6773669B1 (en) 1995-03-10 2004-08-10 Maxcyte, Inc. Flow electroporation chamber and method
US6074605A (en) * 1995-03-10 2000-06-13 Entremed, Inc. Flow electroporation chamber and method
US5720921A (en) * 1995-03-10 1998-02-24 Entremed, Inc. Flow electroporation chamber and method
WO1998014816A1 (en) * 1996-10-02 1998-04-09 Cell Robotics Inc. Microscope with laser port
DE19834612A1 (de) * 1998-07-31 2000-02-24 Dornier Medtech Holding Int Gmbh Verfahren zum intrazellulären Transfer von Oligonukleotiden und Vorrichtung zur Durchführung desselben
JP2002325572A (ja) * 2000-12-25 2002-11-12 Univ Osaka 外来物質の導入方法
US7029916B2 (en) 2001-02-21 2006-04-18 Maxcyte, Inc. Apparatus and method for flow electroporation of biological samples
US7141425B2 (en) 2001-08-22 2006-11-28 Maxcyte, Inc. Apparatus and method for electroporation of biological samples
JP4937904B2 (ja) 2004-05-12 2012-05-23 マックスサイト インコーポレーティッド 制御されたフローエレクトロポレーションチャンバーに関連した方法および装置
US10647960B2 (en) 2005-12-13 2020-05-12 The Trustees Of The University Of Pennsylvania Transcriptome transfer produces cellular phenotype conversion
ES2698600T3 (es) 2005-12-13 2019-02-05 Univ Pennsylvania Métodos para transfectar ácidos nucleicos en células vivas
US9157066B2 (en) 2005-12-13 2015-10-13 The Trustees Of The University Of Pennsylvania Transcriptome transfer produces cellular phenotype conversion
US11178823B2 (en) 2014-04-07 2021-11-23 Premier Citrus Apz, Llc Systems and methods for using light energy to facilitate penetration of substances in plants
EP3386289B1 (de) * 2015-12-10 2020-08-12 Premier Citrus APZ, LLC Verfahren zur verwendung von lichtenergie zur erleichterung des eindringens von substanzen in pflanzen
WO2018085542A1 (en) * 2016-11-03 2018-05-11 President And Fellows Of Harvard College Cellular poration using continuous laser radiation
US10829729B2 (en) 2016-11-03 2020-11-10 President And Fellows Of Harvard College Cellular poration using laser radiation

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3848104A (en) * 1973-04-09 1974-11-12 Avco Everett Res Lab Inc Apparatus for heat treating a surface
JPS5876091A (ja) * 1981-10-28 1983-05-09 Mitsuru Furusawa 真核生物の遺伝形質変換方法

Also Published As

Publication number Publication date
EP0137504A2 (de) 1985-04-17
EP0137504A3 (en) 1987-05-13
EP0137504B1 (de) 1991-01-16
CA1284302C (en) 1991-05-21

Similar Documents

Publication Publication Date Title
DE3483934D1 (de) Verfahren und apparat zum einpflanzen eines fremdstoffes in lebende zelle.
DE69536051D1 (de) Vorrichtung und Verfahren zum In-vivo-Züchten von verschiedenen Gewebezellen
DE3576210D1 (de) Verfahren und apparat zum trennen von blut.
AT397704B (de) Verfahren und vorrichtung zum installieren eines ersatzrohres in eine bestehende rohrleitung
DE68917266D1 (de) Apparat und Verfahren zum Herstellen von absorbierenden Materialien.
DE3577687D1 (de) Verfahren zum bereiten eines ozongases und vorrichtung dafuer.
AT380783B (de) Verfahren und einrichtung zum heben eines patienten
FI102292B1 (fi) Laite ja menetelmä solujen säteilyttämiseksi
DE69417800D1 (de) Verfahren und Apparat zum Zuführen eines Feststoffs in einen Reaktor
DE3884498D1 (de) Programmierbares geraet und verfahren zur pruefung eines programmierbaren geraets.
DE3772025D1 (de) Apparat und verfahren zum zufuehren eines verpackungsfilms.
DE3576737D1 (de) Verfahren zum beobachten eines objektes in einem schmalen spalt und geraet dafuer.
DE68923569D1 (de) Verfahren und Apparat zum Formatieren eines Dokumentes.
DE69535324D1 (de) Mediumdurchdringender Zellkulturträger und seine Verwendungen in eine Kulturmethode und einem Apparat
ZA816471B (en) Method and apparatus for quantitatively determining the level of hemoglobin in a biological sample
DE3878213D1 (de) Verfahren und vorrichtung zum pruefen eines transparenten behaelters.
DE3788079D1 (de) Verfahren und Vorrichtung zum Ausstreichen eines Kulturmediums.
AT382601B (de) Verfahren und vorrichtung zum biologischen aktivierreinigen
DE3776222D1 (de) Verfahren und vorrichtung zum nachweis eines gegebenen teilchenmaterials.
DE68912661D1 (de) Verfahren und Vorrichtung für Zellkultur.
GB2068544B (en) Methods and apparatus for testing biological cells
DE3770556D1 (de) Verfahren und apparat zum festhalten eines steckerstiftes.
AT389506B (de) Verfahren und vorrichtung zum graphitieren von kohlenstoffkoerpern
DE69010354D1 (de) Verbessertes Verfahren und Apparat zum Montieren eines Verbinders.
DK68683A (da) Fremgangsmaade og apparat til brug i forbindelse med en siskabelon

Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee