DK2445928T3 - Kimære influenzaviruslignende partikler omfattende hemagglutinin - Google Patents

Kimære influenzaviruslignende partikler omfattende hemagglutinin Download PDF

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DK2445928T3
DK2445928T3 DK10791119.0T DK10791119T DK2445928T3 DK 2445928 T3 DK2445928 T3 DK 2445928T3 DK 10791119 T DK10791119 T DK 10791119T DK 2445928 T3 DK2445928 T3 DK 2445928T3
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influenza
plant
chimeric
polypeptide
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Manon Couture
Michele Dargis
Pierre-Olivier Lavoie
Louis-Philippe Vezina
Marc-Andre D'aoust
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Medicago Inc
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    • A61K39/00—Medicinal preparations containing antigens or antibodies
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    • A61K39/145—Orthomyxoviridae, e.g. influenza virus
    • A—HUMAN NECESSITIES
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    • A61K39/00—Medicinal preparations containing antigens or antibodies
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    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
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    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
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Claims (11)

  1. KIMÆRE INFLUENZA VIRUSLIGNENDE PARTIKLER OMFATTENDE HEMAGGLUTININ
    1. Nukleinsyre, som kan udtrykkes i en plantevært, omfattende en eller flere regulatoriske regioner, der er funktionsdygtige i en plante, og funktionsmæssigt koblet til en sekvens, der koder for et kim ært influenza-hemagglutinin (HA)-polypeptid omfattende en stem-domæne-cluster (SDC), en hoved-domæne-cluster (HDC) og en transmembran-domæne-cluster (TDC), hvilken ene eller flere regulatoriske regioner omfatter en promoter og en 5’UTR, 3’UTR eller 5’UTR og 3’UTR, og hvori a) SDC’en omfatter et F’ 1-, F’2- og F-subdomæne; b) HDC’en omfatter et receptorbindende (RB)-, El- og E2-subdomæne; c) TDC’en omfatter et transmembran (TmD)- og C-terminal-tail (CT)-subdomæne; og i) hvori RB-subdomænet er fra et første influenza-HA-polypeptid, og SDC- og El- og E2-subdomænet er fra et andet influenza-HA-polypeptid og, ii) hvori det første influenza-HA-polypeptid er fra influenza Hl eller H5, og det andet influenza-HA-polypeptid er fra influenza Hl eller H5, og det andet influenza-HA-polypeptid er afledt af en anden influenzastamme end det første influenza-HA-polypeptid.
  2. 2. Nukleinsyre ifølge krav 1, hvori i) sekvensen, der koder for det kimære influenza-HA-polypeptid, endvidere omfatter en signalpeptidsekvens, der er udvalgt fra gruppen af en HA-nativ-signalpeptidsekvens og en alfalfa-PDI-signalpeptidsekvens, og/eller ii) hvori 5’UTR, 3’UTR eller 5’UTR og 3’UTR opnås fra en plastocyanin-UTR eller CPMV-UTR - og/eller iii) hvori promoteren opnås fra en plastocyanin-regulatorisk region, en Ribulose 1,5-bisphosphat-carboxylase/oxygenase (RuBisCO)-regulatorisk region, en klorofyl a/b-bindende protein (CAB)-regulatorisk region, en CaMV 35S-regulatorisk region, en actin-regulatorisk region, en ubiquitin-regulatorisk region, en triosephosphat-isomerase 1-regulatorisk region, en translationsinitieringsfaktor 4A-regulatorisk region og en ST-LSl-regulatorisk region.
  3. 3. Fremgangsmåde til produktion af kimære influenzaviruslignende partikler (VLP’er) i en plante omfattende: a) indføring af nukleinsyren ifølge krav 1 eller 2 i planten eller andelen deraf og b) inkubering af planten eller andelen deraf under betingelser, der tillader ekspression af nukleinsyren, hvorved VLP’erne produceres, og c) eventuelt høst af planten og oprensning af VLP’eme.
  4. 4. Fremgangsmåde ifølge krav 3, hvori, i trinnet med indføring (trin a), i) nukleinsyren indføres i planten på en transitorisk måde eller ii) nukleinsyren indføres i planten, således at den er stabilt integreret i et genom.
  5. 5. Polypeptid, der kodes for af nukleinsyren ifølge krav 1.
  6. 6. Viruslignende partikel (VLP) omfattende polypeptidet ifølge krav 5.
  7. 7. VLP ifølge krav 6 endvidere omfattende plantespecifikke N-glykaner eller modificerede N-glykaner.
  8. 8. Sammensætning omfattende en effektiv dosis af VLP’en ifølge krav 6 eller 7 og en farmaceutisk acceptabel bærer.
  9. 9. Viruslignende partikel ifølge krav 6 eller 7 eller sammensætning ifølge krav 8 til anvendelse til fremkaldelse af immunitet over for en influenzavirusinfektion hos et subjekt.
  10. 10. Viruslignende partikel eller sammensætning til anvendelse ifølge krav 9, som er egnet til oral, intradermal, intranasal, intramuskulær, intraperitoneal, intravenøs eller subkutan administration til et subjekt.
  11. 11. Plante eller andel deraf omfattende et polypeptid, der kodes for af nukleinsyren ifølge krav 1.
DK10791119.0T 2009-06-24 2010-06-25 Kimære influenzaviruslignende partikler omfattende hemagglutinin DK2445928T3 (da)

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US22016109P 2009-06-24 2009-06-24
PCT/CA2010/000983 WO2010148511A1 (en) 2009-06-24 2010-06-25 Chimeric influenza virus-like particles comprising hemagglutinin

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US (1) US10272148B2 (da)
EP (2) EP2445928B1 (da)
JP (2) JP5871796B2 (da)
KR (1) KR101377725B1 (da)
CN (1) CN102482328B (da)
AU (1) AU2010265766B2 (da)
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ES (1) ES2669303T3 (da)
HK (2) HK1170250A1 (da)
HR (1) HRP20180706T1 (da)
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