DK2358873T4 - Hybride alpha-amylaser - Google Patents

Hybride alpha-amylaser Download PDF

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Publication number
DK2358873T4
DK2358873T4 DK09768480.7T DK09768480T DK2358873T4 DK 2358873 T4 DK2358873 T4 DK 2358873T4 DK 09768480 T DK09768480 T DK 09768480T DK 2358873 T4 DK2358873 T4 DK 2358873T4
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DK
Denmark
Prior art keywords
amylase
hybrid
sequence
seq
residues
Prior art date
Application number
DK09768480.7T
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English (en)
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DK2358873T3 (da
Inventor
Scott D Power
Andrew Shaw
Original Assignee
Danisco Us Inc
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
    • G16B15/20—Protein or domain folding
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B5/00—ICT specially adapted for modelling or simulations in systems biology, e.g. gene-regulatory networks, protein interaction networks or metabolic networks
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Claims (6)

  1. HYBRIDE ALPHA-AMYLASER
    1. Enzymatisk aktiv hybrid a-amylase, der omfatter polypeptidet, der, fra N-terminalen til C-terminalen, har formel (I): A-x-y-B (I), hvor A er en første aminosyresekvens fra en archae-a-amylase; B er en anden aminosyresekvens fra en vildtype-Termamyl-lignende α-amylase eller en variant deraf, der er mindst 80 % sekvensidentisk med den vildtype-Termamyl-lignende α-amylase, hvor den vildtype-Termamyl-lignende α-amylase er mindst 60 % sekvensidentisk med vildtype Bacillus licheniformis alpha-amylase; x er en C-terminalrest fra den første aminosyresekvens; y er en N-terminalrest fra den anden aminosyresekvens; hvor den første og anden aminosyresekvens sammen indeholder 400 til 500 aminosyrerester; hvor mellem 10 % og 80 % af de samlede aminosyrer i den hybride α-amylase er tilført ved hjælp af archae-a-amylasen; hvor rms-afstanden mellem alpha-carboner i resterne x og y sammenlignet med den tredimensionelle vildtype-Termamyl-lignende α-amylasestruktur ikke er mere end 1 A og hvor amylasen omfatter aminosyresekvensen ifølge et hvilket som helst af SEQ ID NO: 1,2 eller 8.
  2. 2. Hybrid a-amylase ifølge krav 1, hvor den hybride α-amylase er oprenset.
  3. 3. Nukleinsyre, der koder for den hybride a-amylase ifølge et hvilket som helst af de foregående krav.
  4. 4. Vektor, der omfatter nukleinsyren ifølge krav 3.
  5. 5. Værtscelle, der omfatter nukleinsyren ifølge krav 3 eller vektoren ifølge krav 4.
  6. 6. Værtscelle ifølge krav 5, hvor værtscellen er en bakterie eller svamp, og eventuelt hvor bakterien er Bacillus sp.
DK09768480.7T 2008-12-15 2009-12-11 Hybride alpha-amylaser DK2358873T4 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US12262808P 2008-12-15 2008-12-15
PCT/US2009/067639 WO2010074999A1 (en) 2008-12-15 2009-12-11 Hybrid alpha-amylases

Publications (2)

Publication Number Publication Date
DK2358873T3 DK2358873T3 (da) 2014-12-08
DK2358873T4 true DK2358873T4 (da) 2018-05-28

Family

ID=41683594

Family Applications (1)

Application Number Title Priority Date Filing Date
DK09768480.7T DK2358873T4 (da) 2008-12-15 2009-12-11 Hybride alpha-amylaser

Country Status (9)

Country Link
US (2) US8841107B2 (da)
EP (1) EP2358873B2 (da)
JP (1) JP5548698B2 (da)
CN (1) CN102245764B (da)
BR (1) BRPI0922258A2 (da)
CA (1) CA2746118A1 (da)
DK (1) DK2358873T4 (da)
MX (1) MX2011006166A (da)
WO (1) WO2010074999A1 (da)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2438205C (en) 2001-02-21 2015-11-03 Diversa Corporation Enzymes having alpha amylase activity and methods of use thereof
US7659102B2 (en) 2001-02-21 2010-02-09 Verenium Corporation Amylases, nucleic acids encoding them and methods for making and using them
US7741092B2 (en) 2002-10-31 2010-06-22 Verenium Corporation Amylases, nucleic acids encoding them and methods for making and using them
EP2358873B2 (en) 2008-12-15 2018-02-21 Danisco US Inc. Hybrid alpha-amylases
CN102884183B (zh) 2010-01-04 2015-09-16 诺维信公司 趋于钙耗竭和酸性pH的α-淀粉酶的稳定化
CN102213716A (zh) * 2011-04-07 2011-10-12 中国水产科学研究院东海水产研究所 一种用酶标法测定海马幼苗组织消化酶活力的方法
EP2997143A1 (en) * 2013-05-17 2016-03-23 Novozymes A/S Polypeptides having alpha amylase activity
US10131926B2 (en) 2013-11-26 2018-11-20 Asymchem Laboratories (Tianjin) Co., Ltd Transaminase and uses thereof
CN107312764B (zh) * 2017-02-21 2020-05-12 南京百斯杰生物工程有限公司 α淀粉酶变体
EP3947664A2 (en) * 2019-03-25 2022-02-09 Basf Se Amylase enzymes
US20220162576A1 (en) * 2019-03-25 2022-05-26 Basf Se Amylase enzymes
WO2023225459A2 (en) 2022-05-14 2023-11-23 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
CN116286575B (zh) * 2023-02-03 2023-10-27 安徽大学绿色产业创新研究院 一种利用枯草芽孢杆菌高效表达生淀粉α-淀粉酶的方法

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EP0625577A1 (en) 1985-08-29 1994-11-23 Genencor International, Inc. Heterologous polypeptides expressed in filamentous fungi, processes for their preparation, and vectors for their preparation
GB8610600D0 (en) 1986-04-30 1986-06-04 Novo Industri As Transformation of trichoderma
DK0562003T4 (da) 1990-12-10 2015-07-13 Danisco Us Inc Forbedret sukkerdannelse fra cellulose ved kloning og amplificering af .-glucosidase-genet fra Tricodermareesei
US5861271A (en) 1993-12-17 1999-01-19 Fowler; Timothy Cellulase enzymes and systems for their expressions
ES2329528T3 (es) 1995-02-03 2009-11-26 Novozymes A/S Metodo para diseñar mutantes alfa-amilasa con propiedades determinadas.
BR9708887B1 (pt) 1996-04-30 2014-10-29 Novozymes As "variante de uma alfa-amilase, uso da mesma, construção de dna, vetor de expressão recombinante, célula de bactéria ou fungo, aditivo e composição detergente".
RU2225118C2 (ru) 1998-04-01 2004-03-10 Даниско А/С Способ изготовления выпечного продукта (варианты), выпечной продукт (варианты), применение немальтогенной экзоамилазы, улучшающая композиция для теста и тесто для выпечного продукта
CA2352046A1 (en) * 1998-12-07 2000-06-15 Novozymes A/S Glucoamylases with n-terminal extensions
US6268328B1 (en) 1998-12-18 2001-07-31 Genencor International, Inc. Variant EGIII-like cellulase compositions
ES2496568T3 (es) 1999-03-30 2014-09-19 Novozymes A/S Variantes de alfa-amilasa
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US7541026B2 (en) * 2007-11-05 2009-06-02 Danisco Us Inc., Genencor Division Alpha-amylase variants with altered properties
JP5520828B2 (ja) * 2007-11-05 2014-06-11 ダニスコ・ユーエス・インク 改変される特徴を有するバシルス種（Ｂａｃｉｌｌｕｓｓｐ．）ＴＳ‐２３アルファ‐アミラーゼ変異体
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Also Published As

Publication number Publication date
US8841107B2 (en) 2014-09-23
JP2012511930A (ja) 2012-05-31
JP5548698B2 (ja) 2014-07-16
EP2358873B1 (en) 2014-09-17
CA2746118A1 (en) 2010-07-01
DK2358873T3 (da) 2014-12-08
US20150017701A1 (en) 2015-01-15
US9803181B2 (en) 2017-10-31
EP2358873B2 (en) 2018-02-21
CN102245764B (zh) 2014-02-26
EP2358873A1 (en) 2011-08-24
BRPI0922258A2 (pt) 2014-11-18
CN102245764A (zh) 2011-11-16
WO2010074999A1 (en) 2010-07-01
US20120021485A1 (en) 2012-01-26
MX2011006166A (es) 2011-10-10

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