DK2358873T3 - Hybride alpha-amylaser - Google Patents

Hybride alpha-amylaser Download PDF

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Publication number
DK2358873T3
DK2358873T3 DK09768480.7T DK09768480T DK2358873T3 DK 2358873 T3 DK2358873 T3 DK 2358873T3 DK 09768480 T DK09768480 T DK 09768480T DK 2358873 T3 DK2358873 T3 DK 2358873T3
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amylase
hybrid
amino acid
wild
residues
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DK09768480.7T
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DK2358873T4 (da
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Scott D Power
Andrew Shaw
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Danisco Us Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B15/00—ICT specially adapted for analysing two-dimensional or three-dimensional molecular structures, e.g. structural or functional relations or structure alignment
    • G16B15/20—Protein or domain folding
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B5/00—ICT specially adapted for modelling or simulations in systems biology, e.g. gene-regulatory networks, protein interaction networks or metabolic networks
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biophysics (AREA)
  • Evolutionary Biology (AREA)
  • Medical Informatics (AREA)
  • Theoretical Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Physiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Claims (17)

  1. HYBRIDE ALPHA-AMYLASER
    1. Enzymatisk aktiv hybrid a-amylase, der omfatter polypeptidet, der, fra N-terminalen til C-terminalen, har formel (I): A-x-y-B (I), hvor A er en første aminosyresekvens fra en archae-a-amylase; B er en anden aminosyresekvens fra en vildtype-Termamyl-lignende α-amylase eller en variant deraf, der er mindst 80 % sekvensidentisk med den vildtype-Termamyl-lignende α-amylase, hvor den vildtype-Termamyl-lignende α-amylase er mindst 60 % sekvensidentisk med vildtype Bacillus licheniformis alpha-amylase; x er en C-terminalrest fra den første aminosyresekvens; y er en N-terminalrest fra den anden aminosyresekvens; hvor den første og anden aminosyresekvens sammen indeholder 400 til 500 aminosyrerester; hvor mellem 10 % og 80 % af de samlede aminosyrer i den hybride α-amylase er tilført ved hjælp af archae-a-amylasen; og hvor rms-afstanden mellem alpha-carboner i resterne x og y sammenlignet med den tredimensionelle vildtype-Termamyl-lignende α-amylasestruktur ikke er mere end 1 A.
  2. 2. Hybrid α-amylase ifølge krav 1, hvor den hybride α-amylase har et ændret niveau af rekombinant ekspression, opløselighed, pH-aktivitetsprofil, substratspecificitet eller specifik aktivitet, sammenlignet med den vildtype-Termamyl-lignende a-amylase.
  3. 3. Hybrid α-amylase ifølge krav 1 eller krav 2, hvor rms-afstanden mellem alpha-carboner i resterne x og y sammenlignet med den vildtype-Termamyl-lignende α-amylases tredimensionelle struktur ikke er mere end 0,5 A.
  4. 4. Hybrid α-amylase ifølge et hvilket som helst af de foregående krav, hvor den første aminosyresekvens A er strukturelt bevaret i den hybride α-amylase sammenlignet med den vildtype-Termamyl-lignende a-amylase.
  5. 5. Hybrid α-amylase ifølge et hvilket som helst af de foregående krav, hvor den vildtype-Termamyl-lignende α-amylase er en Bacillus α-amylase.
  6. 6. Hybrid α-amylase ifølge krav 5, hvor Bacillus α-amylasen er en Bacillus stearothermophilus a-amylase, B. licheniformis α-amylase, B. subtilis α-amylase, Bacillus sp. KSM-K38 α-amylase eller li. halmapalus α-amylase, og eventuelt hvor Bacillus α-amylasen er en B. stearothermophilus a-amylase.
  7. 7. Hybrid α-amylase ifølge et hvilket som helst af de foregående krav, hvor den vildtype-Termamyl-lignende α-amylase er B. stearothermophilus α-amylase, og hvor B er en variant deraf, fra hvilken et stivelsesbindingsdomæne er blevet fjernet fra C-terminalen.
  8. 8. Hybrid α-amylase ifølge et hvilket som helst af de foregående krav, hvor archae-a-amylasen er ultratynd a-amylase.
  9. 9. Hybrid α-amylase ifølge et hvilket som helst af de foregående krav, hvor: (i) den første og anden arninosyrcsckvcns er afledt af amylaser, der er mindre end 60 % sekvensidentisk; og/eller (ii) resterne x og y er i B-domænet; og/eller (iii) den første aminosyresekvens bidrager med mindst 80 % af aminosyreresterne fra B-domænet; og/eller (iv) den første aminosyresekvens omfatter et Zn2+-bindingssite; og/eller (iv) mindst én aminosyre i et Ca2+-bindingssite af den vildtype-Termamyl-lignende a-amylase er erstattet med en aminosyrerest fra den første aminosyresekvens; og/eller (v) den hybride α-amylase har a-amylase-aktivitet, hvor aktiviteten ikke er påvirket af Ca2+-koncentrationen.
  10. 10. Hybrid α-amylase ifølge krav 1, der omfatter aminosyresekvensen vist i en hvilken som helst af SEQ ID NO: 1-8, og eventuelt hvor amylasen omfatter aminosyresekvensen ifølge en hvilken som helst af SEQIDNO: 1,2, 6, 7 eller 8.
  11. 11. Hybrid α-amylase ifølge et hvilket som helst af de foregående krav, hvor den hybride a-amylasen er oprenset.
  12. 12. Nukleinsyre, der koder den hybride α-amylase ifølge et hvilket som helst af de foregående krav.
  13. 13. Vektor, der omfatter nukleinsyren ifølge krav 12.
  14. 14. Værtscelle, der omfatter nukleinsyren ifølge krav 12 eller vektoren ifølge krav 13.
  15. 15. Værtscelle ifølge krav 14, hvor værtscellen er en bakterie eller svamp, og eventuelt hvor bakterien er Bacillus sp.
  16. 16. Fremgangsmåde til at designe nukleinsyren ifølge krav 12, hvilken fremgangsmåde omfatter: (a) aligning af en 3D-struktur af en archae-a-amylase og en vildtype-Termamyl-lignende a-amylase i en computer-implementeret fremgangsmåde, hvor den vildtype-Termamyl-lignende α-amylase er mindst 60 % sekvensidentisk med vildtype Bacillus licheniformis alpha-amylasen; (b) udvælgelse af aminosyreresterne x og y således, at rms-afstanden mellem alpha-carboner i resterne x og y sammenlignet med den tredimensionelle vildtype-Termamyl-lignende a-amylasestruktur ikke er mere end 1 A; og (c) designing af nukleinsyren til at kode for den hybride a-amylase.
  17. 17. Fremgangsmåde ifølge krav 16, hvor: (i) rms-afstanden mellem alpha-carboner i rester x og y sammenlignet med den tredimensionelle vildtype-Termamyl α-amylasestruktur ikke er mere end 0,5 A; og/eller (ii) den computer-implementerede fremgangsmåde omfatter visning af det tredimensionelle strukturelle alignment på en computerskærm.
DK09768480.7T 2008-12-15 2009-12-11 Hybride alpha-amylaser DK2358873T4 (da)

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US12262808P 2008-12-15 2008-12-15
PCT/US2009/067639 WO2010074999A1 (en) 2008-12-15 2009-12-11 Hybrid alpha-amylases

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DK2358873T3 true DK2358873T3 (da) 2014-12-08
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US (2) US8841107B2 (da)
EP (1) EP2358873B2 (da)
JP (1) JP5548698B2 (da)
CN (1) CN102245764B (da)
BR (1) BRPI0922258A2 (da)
CA (1) CA2746118A1 (da)
DK (1) DK2358873T4 (da)
MX (1) MX2011006166A (da)
WO (1) WO2010074999A1 (da)

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AU2003285906A1 (en) 2002-10-31 2004-06-07 Diversa Corporation Amylases, nucleic acids encoding them and methods for making and using them
JP5548698B2 (ja) 2008-12-15 2014-07-16 ダニスコ・ユーエス・インク ハイブリッドアルファ‐アミラーゼ
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MX2011006166A (es) 2011-10-10
EP2358873A1 (en) 2011-08-24
CA2746118A1 (en) 2010-07-01
DK2358873T4 (da) 2018-05-28
US20120021485A1 (en) 2012-01-26
CN102245764B (zh) 2014-02-26
US8841107B2 (en) 2014-09-23
JP2012511930A (ja) 2012-05-31
CN102245764A (zh) 2011-11-16
US9803181B2 (en) 2017-10-31
US20150017701A1 (en) 2015-01-15
EP2358873B2 (en) 2018-02-21
WO2010074999A1 (en) 2010-07-01
EP2358873B1 (en) 2014-09-17
BRPI0922258A2 (pt) 2014-11-18
JP5548698B2 (ja) 2014-07-16

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