DK2333044T3 - Farmaceutisk kompatibel fremgangsmåde til oprensning af intakte bakterielle miniceller - Google Patents

Farmaceutisk kompatibel fremgangsmåde til oprensning af intakte bakterielle miniceller Download PDF

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Publication number
DK2333044T3
DK2333044T3 DK10180971.3T DK10180971T DK2333044T3 DK 2333044 T3 DK2333044 T3 DK 2333044T3 DK 10180971 T DK10180971 T DK 10180971T DK 2333044 T3 DK2333044 T3 DK 2333044T3
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DK
Denmark
Prior art keywords
minicells
minicell
bacterial
cells
parent
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DK10180971.3T
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English (en)
Inventor
Himanshu Brahmbhatt
Jennifer Macdiarmid
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Engeneic Molecular Delivery Pty Ltd
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Publication of DK2333044T3 publication Critical patent/DK2333044T3/da

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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/66—Microorganisms or materials therefrom
    • A61K35/74—Bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Molecular Biology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Claims (3)

  1. FARMACEUTISK KOMPATIBEL FREMGANGSMÅDE TIL OPRENSNING AF INTAKTE BAKTERIELLE MINICELLER
    1. Fremstilling af en oprenset sammensætning af bakterielt afledte miniceller, som i alt væsentligt er fri for frie endotoksiner, hvor minicellerne oprenses med en oprensningsfremgangsmåde, som omfatter underkastelse af minicellerne for en tilstand, som inducerer moderbakterieceilerne til at antage en filamentøs form, og derefter filtrering af prøven, og de frie endotoksiner fjernes ved hjælp af anti-lipid A antistoffer.
  2. 2. Fremstilling af bakterielt afledte miniceller ifølge krav 1, som indeholder færre end omkring 1 kontaminerende moderbakteriecelle per 1010 miniceller.
  3. 3. Fremstilling af bakterielt afledte miniceller ifølge krav 2, som indeholder færre end omkring 1 kontaminerende moderbakteriecelle per 1Qrl miniceller.
DK10180971.3T 2003-06-24 2004-06-23 Farmaceutisk kompatibel fremgangsmåde til oprensning af intakte bakterielle miniceller DK2333044T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/602,021 US7611885B2 (en) 2003-06-24 2003-06-24 Pharmaceutically compatible method for purifying intact bacterial minicells
EP08101712A EP1947171B1 (en) 2003-06-24 2004-06-23 Purified bacterial minicells

Publications (1)

Publication Number Publication Date
DK2333044T3 true DK2333044T3 (da) 2016-12-12

Family

ID=33539477

Family Applications (3)

Application Number Title Priority Date Filing Date
DK10180971.3T DK2333044T3 (da) 2003-06-24 2004-06-23 Farmaceutisk kompatibel fremgangsmåde til oprensning af intakte bakterielle miniceller
DK08101712.1T DK1947171T3 (da) 2003-06-24 2004-06-23 Oprensede bakterieminiceller
DK04743793.4T DK1636344T3 (da) 2003-06-24 2004-06-23 Farmaceutisk kompatibel fremgangsmåde til oprensning af intakte bakterielle miniceller

Family Applications After (2)

Application Number Title Priority Date Filing Date
DK08101712.1T DK1947171T3 (da) 2003-06-24 2004-06-23 Oprensede bakterieminiceller
DK04743793.4T DK1636344T3 (da) 2003-06-24 2004-06-23 Farmaceutisk kompatibel fremgangsmåde til oprensning af intakte bakterielle miniceller

Country Status (19)

Country Link
US (4) US7611885B2 (da)
EP (3) EP1636344B1 (da)
JP (1) JP4834544B2 (da)
CN (2) CN1809629B (da)
AT (2) ATE513032T1 (da)
AU (2) AU2004249970B2 (da)
CA (2) CA2780843C (da)
CY (1) CY1118218T1 (da)
DE (1) DE602004026051D1 (da)
DK (3) DK2333044T3 (da)
ES (3) ES2602572T3 (da)
HK (3) HK1089207A1 (da)
HU (1) HUE030642T2 (da)
MX (1) MXPA05013620A (da)
NZ (1) NZ544653A (da)
PL (2) PL1947171T3 (da)
PT (2) PT2333044T (da)
SI (1) SI2333044T1 (da)
WO (1) WO2004113507A1 (da)

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ES2380612T3 (es) * 2001-10-15 2012-05-16 Engeneic Molecular Delivery Pty Ltd. Minicélulas intactas como vectores para transferencia de ADN y terapia génica in vitro e in vivo
US7611885B2 (en) * 2003-06-24 2009-11-03 Engeneic Molecular Delivery Pty, Ltd. Pharmaceutically compatible method for purifying intact bacterial minicells
EP1694361B1 (en) 2003-12-09 2011-03-16 EnGeneIC Molecular Delivery Pty Ltd. Targeted gene delivery to non-phagocytic mammalian cells via bacterially derived intact minicells
US8772013B2 (en) * 2004-02-02 2014-07-08 Engeneic Molecular Delivery Pty Ltd Methods for targeted in vitro and in vivo drug delivery to mammalian cells via bacterially derived intact minicells
EP1718338B1 (en) 2004-02-02 2015-05-06 EnGeneIC Molecular Delivery Pty Ltd. Compositions and methods for targeted in vitro and in vivo drug delivery to mammalian cells via bacterially derived intact minicells
DK2386640T3 (da) 2004-08-26 2015-04-27 Engeneic Molecular Delivery Pty Ltd Afgivelse af funktionelle nukleinsyrer til pattedyrceller via bakterielt afledte, intakte miniceller
HUE033007T2 (en) 2006-06-23 2017-11-28 Engeneic Molecular Delivery Pty Ltd Targeted drug, therapeutic nucleic acid and functional nucleic acid delivery to mammalian cells via intact killed bacterial cells
PT2865755T (pt) 2007-03-30 2017-05-26 Engeneic Molecular Delivery Pty Ltd Minicélulas intactas derivadas de bactéria compreendendo arn regulatório
KR20180013993A (ko) 2008-06-25 2018-02-07 벡션 테라퓨틱스 엘엘씨 조절된 유전자 자살 메커니즘 조성물 및 방법
US8653236B2 (en) 2008-07-17 2014-02-18 Inter-K Pty Limited Therapeutic agents
WO2010129779A1 (en) 2009-05-07 2010-11-11 Biomerieux, Inc. Methods for antimicrobial resistance determination
US20120142089A1 (en) * 2010-12-01 2012-06-07 Samsung Electronics Co., Ltd. Method of separating target cell in biological sample
US9624474B2 (en) * 2010-12-01 2017-04-18 Samsung Electronics Co, Ltd. Method of separating target cell in biological sample
SG10201601349XA (en) 2011-12-13 2016-03-30 Engeneic Molecular Delivery Pty Ltd Bacterially derived, intact minicells for delivery of therapeutic agents to brain tumors
EP2807180B1 (en) 2012-01-24 2018-03-28 InterK Peptide Therapeutics Limited Peptide agents for cancer therapy
EA032740B1 (ru) 2013-10-04 2019-07-31 Энджинеик Молекьюлар Деливери Пти Лтд Способ лечения опухоли у индивидуума и набор для осуществления способа
US10328159B2 (en) 2014-10-03 2019-06-25 Engenic Molecular Delivery Pty Ltd. Enhanced loading of intact, bacterially derived vesicles with small molecule compounds
CN113577323B (zh) 2015-08-04 2024-02-20 瓦克星治疗有限责任公司 基于细菌小细胞的生物药物的电离辐射灭菌以及使用方法
SG11201902940RA (en) 2016-10-06 2019-05-30 Engeneic Molecular Delivery Pty Ltd Bacterial minicells for delivering nucleic acid adjuvants and methods of using the same
JP2021532106A (ja) 2018-07-23 2021-11-25 エンジーンアイシー モレキュラー デリバリー ピーティーワイ リミテッド 細菌由来ミニ細胞を含む組成物およびそれを使用する方法
US20230242867A1 (en) * 2020-06-17 2023-08-03 Flagship Pioneering Innovations Vi, Llc Methods for manufacturing adas
CN113186100B (zh) * 2021-05-11 2022-10-04 中国科学院青岛生物能源与过程研究所 一种富集污水微生物以用于单细胞精度表型测量和分选的试剂盒及方法

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ES2380612T3 (es) 2001-10-15 2012-05-16 Engeneic Molecular Delivery Pty Ltd. Minicélulas intactas como vectores para transferencia de ADN y terapia génica in vitro e in vivo
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US7611885B2 (en) * 2003-06-24 2009-11-03 Engeneic Molecular Delivery Pty, Ltd. Pharmaceutically compatible method for purifying intact bacterial minicells
EP1694361B1 (en) * 2003-12-09 2011-03-16 EnGeneIC Molecular Delivery Pty Ltd. Targeted gene delivery to non-phagocytic mammalian cells via bacterially derived intact minicells
US8772013B2 (en) * 2004-02-02 2014-07-08 Engeneic Molecular Delivery Pty Ltd Methods for targeted in vitro and in vivo drug delivery to mammalian cells via bacterially derived intact minicells
DK2386640T3 (da) * 2004-08-26 2015-04-27 Engeneic Molecular Delivery Pty Ltd Afgivelse af funktionelle nukleinsyrer til pattedyrceller via bakterielt afledte, intakte miniceller
KR100967868B1 (ko) 2004-12-17 2010-07-05 베쓰 이스라엘 디코니스 메디칼 센터 박테리아 매개 유전자 침묵을 위한 조성물 및 이것을이용하는 방법
HUE033007T2 (en) * 2006-06-23 2017-11-28 Engeneic Molecular Delivery Pty Ltd Targeted drug, therapeutic nucleic acid and functional nucleic acid delivery to mammalian cells via intact killed bacterial cells

Also Published As

Publication number Publication date
US10076545B2 (en) 2018-09-18
MXPA05013620A (es) 2006-02-24
DK1947171T3 (da) 2011-09-19
AU2004249970B2 (en) 2009-09-10
AU2004249970A1 (en) 2004-12-29
US20110177185A1 (en) 2011-07-21
EP2333044B1 (en) 2016-08-17
ATE461269T1 (de) 2010-04-15
US8449877B2 (en) 2013-05-28
CN103361286A (zh) 2013-10-23
SI2333044T1 (sl) 2016-12-30
ES2602572T3 (es) 2017-02-21
EP1636344A1 (en) 2006-03-22
CA2780843C (en) 2013-12-10
CN1809629B (zh) 2013-06-12
CY1118218T1 (el) 2017-06-28
JP4834544B2 (ja) 2011-12-14
HK1089207A1 (en) 2006-11-24
PL2333044T3 (pl) 2017-02-28
US20070241067A1 (en) 2007-10-18
EP1636344A4 (en) 2006-07-19
PT2333044T (pt) 2016-11-21
JP2007520201A (ja) 2007-07-26
NZ544653A (en) 2009-02-28
DK1636344T3 (da) 2010-05-31
CA2780843A1 (en) 2012-08-13
CA2530055C (en) 2012-10-02
ES2367535T3 (es) 2011-11-04
US7611885B2 (en) 2009-11-03
CN1809629A (zh) 2006-07-26
WO2004113507A9 (en) 2006-08-24
US20040265994A1 (en) 2004-12-30
CN103361286B (zh) 2016-12-28
HK1158261A1 (zh) 2012-07-13
US20130236422A1 (en) 2013-09-12
CA2530055A1 (en) 2004-12-29
DE602004026051D1 (de) 2010-04-29
US8003091B2 (en) 2011-08-23
ES2342830T3 (es) 2010-07-15
AU2009239519A1 (en) 2009-12-10
AU2009239519B2 (en) 2010-04-22
HUE030642T2 (en) 2017-06-28
PT1947171E (pt) 2011-09-07
HK1121190A1 (en) 2009-04-17
EP1636344B1 (en) 2010-03-17
ATE513032T1 (de) 2011-07-15
WO2004113507A1 (en) 2004-12-29
EP1947171B1 (en) 2011-06-15
PL1947171T3 (pl) 2011-12-30
EP2333044A1 (en) 2011-06-15
EP1947171A1 (en) 2008-07-23

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