DK2203565T3 - Muterede acetohydroxysyresyntasegener i brassica - Google Patents

Muterede acetohydroxysyresyntasegener i brassica Download PDF

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DK2203565T3
DK2203565T3 DK08836084.7T DK08836084T DK2203565T3 DK 2203565 T3 DK2203565 T3 DK 2203565T3 DK 08836084 T DK08836084 T DK 08836084T DK 2203565 T3 DK2203565 T3 DK 2203565T3
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ahas
gene
plant
seq
herbicide
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DK08836084.7T
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Christian Schopke
Greg F W Gocal
Keith Walker
Peter R Beetham
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Cibus Europe Bv
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8274Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for herbicide resistance
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    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1022Transferases (2.) transferring aldehyde or ketonic groups (2.2)
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    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
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  • Engineering & Computer Science (AREA)
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  • General Engineering & Computer Science (AREA)
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  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Catching Or Destruction (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Plural Heterocyclic Compounds (AREA)

Claims (4)

1. Fremgangsmåde til fremstilling af en herbicidresistent plante, hvilken fremgangsmåde omfatter: (a) at indføre i en plantecelle en enkelstrenget genreparation-oligonukleobase (GRON) med en målrettet mutation i et acetohydroxysyre-syntasegen (AHAS) til fremstilling af en plantecelle med: (i) et AHAS-gen, der udtrykker et AHAS-protein omfattende en substitution af alanin med valin eller asparaginsyre i en position svarende til position 205 af SEQ ID NO: 1 og (ii) et AHAS-gen, der udtrykker et AHAS-protein omfattende en substitution af serin med asparagin i en position svarende til position 653 af SEQ ID NO: 1; (b) at identificere en plantecelle med i det væsentlige normal vækst og katalytisk aktivitet i forhold til en tilsvarende vildtypeplantecelle i nærvær af et AHAS-hæmmende herbicid; og (c) at regenerere en ikke-transgen herbicidresistent plante med et muteret AHAS-gen fra plantecellen.
2. Fremgangsmåde ifølge krav 1, hvorved det AHAS-hæmmende herbicid udvælges fra gruppen bestående af herbicider af: imidazolinon, sulfonylurea, triazolopyrimidin, pyrimidinylthiobenzoat, sulfonylamino-carbonyltriazolinon og blandinger deraf.
3. Fremgangsmåde ifølge krav 1 eller 2, hvorved AHAS-genet koder for et protein omfattende 70 % identitet eller derover med en eller flere af aminosy-resekvenserne fra figur 2.
4. Fremgangsmåde ifølge krav 1, 2 eller 3, hvorved den herbicidresistente plante udvælges fra gruppen bestående af sukkerroe, raps og canola; og hvorved herbicidet er et sulfonylurea-herbicid.
DK08836084.7T 2007-10-05 2008-10-03 Muterede acetohydroxysyresyntasegener i brassica DK2203565T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US97794407P 2007-10-05 2007-10-05
PCT/US2008/078798 WO2009046334A1 (en) 2007-10-05 2008-10-03 Mutated acetohydroxyacid synthase genes in brassica

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DK2203565T3 true DK2203565T3 (da) 2015-12-07

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DK13175393.1T DK2700722T3 (da) 2007-10-05 2008-10-03 Muterede acetohydroxysyresyntasegener i brassica
DK13175389.9T DK2700721T3 (da) 2007-10-05 2008-10-03 Muterede acetohydroxysyresyntasegener i brassica
DK08836084.7T DK2203565T3 (da) 2007-10-05 2008-10-03 Muterede acetohydroxysyresyntasegener i brassica

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US (3) US20090205064A1 (da)
EP (4) EP2203565B1 (da)
JP (4) JP5745849B2 (da)
CN (5) CN108342376A (da)
AU (1) AU2008308530A1 (da)
BR (3) BR122018070230B1 (da)
CA (1) CA2701624C (da)
DK (3) DK2700722T3 (da)
EA (3) EA026107B1 (da)
LT (2) LT2700722T (da)
PL (3) PL2700722T3 (da)
UA (2) UA103887C2 (da)
WO (1) WO2009046334A1 (da)

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EP2700722A2 (en) 2014-02-26
WO2009046334A4 (en) 2009-05-28
JP2015204828A (ja) 2015-11-19
JP2017140024A (ja) 2017-08-17
EP2700722B1 (en) 2019-06-19
CA2701624C (en) 2019-08-20
DK2700722T3 (da) 2019-09-16
EA201691664A1 (ru) 2017-05-31
EP2203565B1 (en) 2015-08-26
CN115044593A (zh) 2022-09-13
JP2019146587A (ja) 2019-09-05
CN108342376A (zh) 2018-07-31
UA103887C2 (ru) 2013-12-10
PL2700722T3 (pl) 2019-12-31
UA119225C2 (uk) 2019-05-27
EA202092479A3 (ru) 2021-06-30
WO2009046334A1 (en) 2009-04-09
US20120178628A1 (en) 2012-07-12
EA026107B1 (ru) 2017-03-31
US20090205064A1 (en) 2009-08-13
EP2203565A4 (en) 2010-10-27
JP2010539986A (ja) 2010-12-24
AU2008308530A1 (en) 2009-04-09
CN102405846A (zh) 2012-04-11
CN108130336A (zh) 2018-06-08
JP6529528B2 (ja) 2019-06-12
EA036997B1 (ru) 2021-01-25
LT2700722T (lt) 2019-09-25
DK2700721T3 (da) 2019-04-01
LT2700721T (lt) 2019-04-10
PL2203565T3 (pl) 2016-02-29
EP2203565A1 (en) 2010-07-07
EP3584325A1 (en) 2019-12-25
JP6833903B2 (ja) 2021-02-24
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