DK2195426T3 - System til opsamling og modificering af store stykker af genomisk DNA og konstruktion af organismer med syntetiske kloroplastre. - Google Patents

System til opsamling og modificering af store stykker af genomisk DNA og konstruktion af organismer med syntetiske kloroplastre.

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Publication number
DK2195426T3
DK2195426T3 DK08836521.8T DK08836521T DK2195426T3 DK 2195426 T3 DK2195426 T3 DK 2195426T3 DK 08836521 T DK08836521 T DK 08836521T DK 2195426 T3 DK2195426 T3 DK 2195426T3
Authority
DK
Denmark
Prior art keywords
collecting
genomic dna
large pieces
chloroplasts
modifying large
Prior art date
Application number
DK08836521.8T
Other languages
Danish (da)
English (en)
Inventor
Michael Mendez
Bryan O'neill
Kari Mikkelson
Original Assignee
Sapphire Energy Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sapphire Energy Inc filed Critical Sapphire Energy Inc
Application granted granted Critical
Publication of DK2195426T3 publication Critical patent/DK2195426T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Nutrition Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK08836521.8T 2007-10-05 2008-10-06 System til opsamling og modificering af store stykker af genomisk DNA og konstruktion af organismer med syntetiske kloroplastre. DK2195426T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US97802407P 2007-10-05 2007-10-05
PCT/US2008/011540 WO2009045550A2 (en) 2007-10-05 2008-10-06 System for capturing and modifying large pieces of genomic dna and constructing organisms with synthetic chloroplasts

Publications (1)

Publication Number Publication Date
DK2195426T3 true DK2195426T3 (da) 2013-09-08

Family

ID=40042337

Family Applications (1)

Application Number Title Priority Date Filing Date
DK08836521.8T DK2195426T3 (da) 2007-10-05 2008-10-06 System til opsamling og modificering af store stykker af genomisk DNA og konstruktion af organismer med syntetiske kloroplastre.

Country Status (12)

Country Link
EP (1) EP2195426B1 (https=)
JP (1) JP5666908B2 (https=)
KR (1) KR101389254B1 (https=)
CN (1) CN101939423A (https=)
AU (1) AU2008307471B2 (https=)
CA (1) CA2698627A1 (https=)
DK (1) DK2195426T3 (https=)
GB (2) GB2460351B (https=)
IL (1) IL204250A (https=)
MX (1) MX2010003157A (https=)
NZ (1) NZ583682A (https=)
WO (1) WO2009045550A2 (https=)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5618413B2 (ja) 2007-10-08 2014-11-05 シンセティック ゲノミクス、インク． 大型核酸のアッセンブリー
SG179157A1 (en) * 2009-09-15 2012-05-30 Sapphire Energy Inc A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp.
CN104277974B (zh) * 2013-07-03 2017-09-15 中国科学院植物研究所 一种分离产氢衣藻叶绿体的方法
CN104419701B (zh) * 2013-08-29 2019-01-11 天津大学 多片段dna的酵母快速组装方法
JP6226464B2 (ja) * 2013-09-30 2017-11-08 花王株式会社 チオエステラーゼ改変体を用いた脂質の製造方法
ES2778727T3 (es) * 2014-08-25 2020-08-11 Geneweave Biosciences Inc Partículas de transducción no replicativas y sistemas indicadores basados en partículas de transducción
US11008602B2 (en) 2017-12-20 2021-05-18 Roche Molecular Systems, Inc. Non-replicative transduction particles and transduction particle-based reporter systems
CN109536525B (zh) * 2019-02-20 2019-08-23 中国科学院烟台海岸带研究所 一种杜氏盐藻叶绿体同源重组空载体及其应用
CN110669789B (zh) * 2019-12-04 2020-04-14 中国科学院烟台海岸带研究所 一种雨生红球藻叶绿体表达系统及其应用
CN111440733A (zh) * 2020-02-07 2020-07-24 天津大学 产松油醇的重组酿酒酵母及构建方法及应用
CN115807021A (zh) * 2022-08-01 2023-03-17 深圳大学 一种莱茵衣藻人工叶绿体基因组合成组装与功能测试的方法
CN115948450B (zh) * 2022-08-01 2024-02-13 深圳大学 一种莱茵衣藻叶绿体-酿酒酵母-大肠杆菌穿梭载体及其构建方法与应用
CN116218750B (zh) * 2022-10-14 2024-11-26 天津科技大学 一种解淀粉芽孢杆菌底盘菌及其构建方法与应用
CN118256412B (zh) * 2024-04-01 2024-09-27 湖北大学 运动发酵单胞菌的工程菌株、制备方法及应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7129391B1 (en) * 1988-09-26 2006-10-31 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
US5866404A (en) * 1995-12-06 1999-02-02 Yale University Yeast-bacteria shuttle vector
US5976846A (en) * 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
NZ502742A (en) * 1997-08-07 2004-04-30 Univ Auburn Universal chloroplast integration and expression vectors, transformed plants and products thereof
AU5217599A (en) * 1998-07-27 2000-02-21 Genotypes, Inc. Automatic eukaryotic artificial chromosome vector
AU5597200A (en) * 1999-06-07 2000-12-28 Cell Genesys, Inc. Hybrid yeast-bacteria cloning system and uses thereof
CN1997743B (zh) * 2004-03-03 2011-12-21 国立大学法人奈良先端科学技术大学院大学 通过叶绿体技术提高植物生产能力的方法
BRPI0511046A (pt) * 2004-05-10 2007-11-27 Basf Plant Science Gmbh métodos para produzir uma construção de expressão múltipla, para produzir alimento ou produtos alimentìcios, produtos farmacêuticos ou produtos quìmicos, e uma planta ou célula de planta transgênica, construção de expressão múltipla, microorganismo,e, uso de um microorganismo, de uma célula de planta, de um tecido de planta, de um orgão de planta, ou de uma parte de uma planta
EP2171085A4 (en) * 2007-06-01 2010-07-14 Sapphire Energy SCREENING OF GENETICALLY MODIFIED PHOTOSYNTHETIC ORGANISMS WITH HIGH THROUGHPUT
US20090209015A1 (en) * 2008-02-15 2009-08-20 Ramesha Chakkodabylu S Compositions and methods for production of biofuels

Also Published As

Publication number Publication date
KR101389254B1 (ko) 2014-04-24
EP2195426A4 (en) 2010-12-01
WO2009045550A3 (en) 2009-07-30
AU2008307471A1 (en) 2009-04-09
KR20100085930A (ko) 2010-07-29
GB2453648C (en) 2012-12-05
IL204250A (en) 2012-12-31
GB2453648A8 (en) 2010-11-17
GB0913302D0 (en) 2009-09-02
MX2010003157A (es) 2010-05-21
GB2460351B (en) 2011-03-30
WO2009045550A2 (en) 2009-04-09
GB0818260D0 (en) 2008-11-12
EP2195426B1 (en) 2013-06-12
GB2453648C2 (en) 2012-12-19
JP2010539979A (ja) 2010-12-24
JP5666908B2 (ja) 2015-02-12
NZ583682A (en) 2012-07-27
EP2195426A2 (en) 2010-06-16
GB2460351A (en) 2009-12-02
GB2453648B (en) 2011-01-05
CN101939423A (zh) 2011-01-05
AU2008307471B2 (en) 2013-05-02
GB2453648A (en) 2009-04-15
CA2698627A1 (en) 2009-04-09

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