CA2698627A1 - System for capturing and modifying large pieces of genomic dna and constructing organisms with synthetic chloroplasts - Google Patents

Info

Publication number

CA2698627A1

CA2698627A1 CA2698627A CA2698627A CA2698627A1 CA 2698627 A1 CA2698627 A1 CA 2698627A1 CA 2698627 A CA2698627 A CA 2698627A CA 2698627 A CA2698627 A CA 2698627A CA 2698627 A1 CA2698627 A1 CA 2698627A1

Authority

CA

Canada

Prior art keywords

vector

organism

chloroplast

dna

genome

Prior art date

2007-10-05

Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)

Abandoned

Links

• Espacenet
• Global Dossier
• CIPO
• Discuss

• 210000003763 chloroplast Anatomy 0.000 title claims abstract description 257
• 238000000034 method Methods 0.000 claims abstract description 218
• 108090000623 proteins and genes Proteins 0.000 claims abstract description 123
• 230000037361 pathway Effects 0.000 claims abstract description 48
• 238000002744 homologous recombination Methods 0.000 claims abstract description 45
• 230000006801 homologous recombination Effects 0.000 claims abstract description 45
• 210000003463 organelle Anatomy 0.000 claims abstract description 29
• 239000013605 shuttle vector Substances 0.000 claims abstract description 19
• 239000013598 vector Substances 0.000 claims description 415
• 108020004414 DNA Proteins 0.000 claims description 298
• 210000004027 cell Anatomy 0.000 claims description 230
• 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 157
• 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 157
• 150000007523 nucleic acids Chemical class 0.000 claims description 109
• 102000039446 nucleic acids Human genes 0.000 claims description 107
• 108020004707 nucleic acids Proteins 0.000 claims description 107
• 239000003550 marker Substances 0.000 claims description 91
• 230000014509 gene expression Effects 0.000 claims description 86
• 241000195597 Chlamydomonas reinhardtii Species 0.000 claims description 74
• 150000003505 terpenes Chemical class 0.000 claims description 72
• 102000004190 Enzymes Human genes 0.000 claims description 69
• 108090000790 Enzymes Proteins 0.000 claims description 69
• 230000000243 photosynthetic effect Effects 0.000 claims description 63
• 230000001580 bacterial effect Effects 0.000 claims description 55
• 108700031407 Chloroplast Genes Proteins 0.000 claims description 53
• 238000004519 manufacturing process Methods 0.000 claims description 52
• 241000195493 Cryptophyta Species 0.000 claims description 51
• 230000009466 transformation Effects 0.000 claims description 45
• 238000012986 modification Methods 0.000 claims description 41
• 230000004048 modification Effects 0.000 claims description 41
• 230000035772 mutation Effects 0.000 claims description 39
• 230000006870 function Effects 0.000 claims description 36
• 241001474374 Blennius Species 0.000 claims description 35
• 239000012634 fragment Substances 0.000 claims description 33
• 230000006798 recombination Effects 0.000 claims description 30
• 238000012217 deletion Methods 0.000 claims description 29
• 230000037430 deletion Effects 0.000 claims description 29
• 238000005215 recombination Methods 0.000 claims description 29
• 230000001131 transforming effect Effects 0.000 claims description 27
• 230000002792 vascular Effects 0.000 claims description 27
• 108091033319 polynucleotide Proteins 0.000 claims description 26
• 102000040430 polynucleotide Human genes 0.000 claims description 26
• 239000002157 polynucleotide Substances 0.000 claims description 26
• 230000008707 rearrangement Effects 0.000 claims description 26
• 230000010076 replication Effects 0.000 claims description 26
• 108020004998 Chloroplast DNA Proteins 0.000 claims description 24
• 235000007586 terpenes Nutrition 0.000 claims description 24
• 230000003115 biocidal effect Effects 0.000 claims description 23
• 230000010354 integration Effects 0.000 claims description 20
• 235000014113 dietary fatty acids Nutrition 0.000 claims description 19
• 239000000194 fatty acid Substances 0.000 claims description 19
• 229930195729 fatty acid Natural products 0.000 claims description 19
• 150000004665 fatty acids Chemical class 0.000 claims description 19
• 229930195733 hydrocarbon Natural products 0.000 claims description 19
• 150000002430 hydrocarbons Chemical class 0.000 claims description 19
• OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 17
• 241000588724 Escherichia coli Species 0.000 claims description 17
• 229910052799 carbon Inorganic materials 0.000 claims description 16
• 239000000203 mixture Substances 0.000 claims description 16
• 210000002230 centromere Anatomy 0.000 claims description 14
• 238000001727 in vivo Methods 0.000 claims description 14
• 239000002028 Biomass Substances 0.000 claims description 13
• 102100023072 Neurolysin, mitochondrial Human genes 0.000 claims description 13
• 230000029553 photosynthesis Effects 0.000 claims description 13
• 238000010672 photosynthesis Methods 0.000 claims description 13
• 239000003242 anti bacterial agent Substances 0.000 claims description 12
• 230000000593 degrading effect Effects 0.000 claims description 12
• 230000003362 replicative effect Effects 0.000 claims description 10
• 230000008685 targeting Effects 0.000 claims description 9
• 150000001875 compounds Chemical class 0.000 claims description 6
• 230000028327 secretion Effects 0.000 claims description 6
• 108091026890 Coding region Proteins 0.000 claims description 5
• 108091093105 Nuclear DNA Proteins 0.000 claims description 5
• 230000001965 increasing effect Effects 0.000 claims description 5
• 238000003780 insertion Methods 0.000 claims description 5
• 230000037431 insertion Effects 0.000 claims description 5
• 241000144833 Halomonas salina Species 0.000 claims 11
• 238000010230 functional analysis Methods 0.000 abstract description 2
• 239000000047 product Substances 0.000 description 92
• 229940088598 enzyme Drugs 0.000 description 62
• 241000196324 Embryophyta Species 0.000 description 60
• 241000894006 Bacteria Species 0.000 description 42
• 238000011049 filling Methods 0.000 description 31
• 239000013612 plasmid Substances 0.000 description 31
• 238000010367 cloning Methods 0.000 description 27
• 241000195633 Dunaliella salina Species 0.000 description 24
• 108091028043 Nucleic acid sequence Proteins 0.000 description 22
• 239000000446 fuel Substances 0.000 description 21
• 239000002773 nucleotide Substances 0.000 description 19
• 125000003729 nucleotide group Chemical group 0.000 description 19
• 230000001105 regulatory effect Effects 0.000 description 19
• 229930027917 kanamycin Natural products 0.000 description 18
• 229960000318 kanamycin Drugs 0.000 description 18
• SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 18
• 229930182823 kanamycin A Natural products 0.000 description 18
• 239000002609 medium Substances 0.000 description 18
• 101150050575 URA3 gene Proteins 0.000 description 17
• 238000007792 addition Methods 0.000 description 17
• KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 16
• 239000002245 particle Substances 0.000 description 16
• 210000005253 yeast cell Anatomy 0.000 description 16
• 229920001817 Agar Polymers 0.000 description 15
• 239000008272 agar Substances 0.000 description 15
• 238000010353 genetic engineering Methods 0.000 description 15
• 239000000243 solution Substances 0.000 description 15
• 108020004705 Codon Proteins 0.000 description 14
• 101150009006 HIS3 gene Proteins 0.000 description 14
• 230000012010 growth Effects 0.000 description 14
• 101710121765 Endo-1,4-beta-xylanase Proteins 0.000 description 12
• 108700039887 Essential Genes Proteins 0.000 description 12
• 239000002585 base Substances 0.000 description 12
• 229920001184 polypeptide Polymers 0.000 description 12
• 108090000765 processed proteins & peptides Proteins 0.000 description 12
• 102000004196 processed proteins & peptides Human genes 0.000 description 12
• 101150075980 psbA gene Proteins 0.000 description 12
• UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 12
• 239000004215 Carbon black (E152) Substances 0.000 description 11
• XIXADJRWDQXREU-UHFFFAOYSA-M lithium acetate Chemical compound [Li+].CC([O-])=O XIXADJRWDQXREU-UHFFFAOYSA-M 0.000 description 11
• 229940071257 lithium acetate Drugs 0.000 description 11
• 102000004169 proteins and genes Human genes 0.000 description 11
• 101710165761 (2E,6E)-farnesyl diphosphate synthase Proteins 0.000 description 10
• 101710156207 Farnesyl diphosphate synthase Proteins 0.000 description 10
• 102100035111 Farnesyl pyrophosphate synthase Human genes 0.000 description 10
• 101710125754 Farnesyl pyrophosphate synthase Proteins 0.000 description 10
• 101710089428 Farnesyl pyrophosphate synthase erg20 Proteins 0.000 description 10
• 101710150389 Probable farnesyl diphosphate synthase Proteins 0.000 description 10
• 238000004458 analytical method Methods 0.000 description 10
• -1 phosphinotricin Chemical compound 0.000 description 10
• 210000002706 plastid Anatomy 0.000 description 10
• LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 9
• 239000002243 precursor Substances 0.000 description 9
• 235000018102 proteins Nutrition 0.000 description 9
• 108020005345 3' Untranslated Regions Proteins 0.000 description 8
• 101150096273 ADE2 gene Proteins 0.000 description 8
• 108010001817 Endo-1,4-beta Xylanases Proteins 0.000 description 8
• 230000008901 benefit Effects 0.000 description 8
• 238000005119 centrifugation Methods 0.000 description 8
• 238000006243 chemical reaction Methods 0.000 description 8
• 239000006166 lysate Substances 0.000 description 8
• 238000000844 transformation Methods 0.000 description 8
• 108020003589 5' Untranslated Regions Proteins 0.000 description 7
• 108700010070 Codon Usage Proteins 0.000 description 7
• 230000000694 effects Effects 0.000 description 7
• 238000005516 engineering process Methods 0.000 description 7
• 230000002538 fungal effect Effects 0.000 description 7
• 230000008569 process Effects 0.000 description 7
• IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 6
• PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 6
• HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
• 238000000137 annealing Methods 0.000 description 6
• 238000000338 in vitro Methods 0.000 description 6
• 238000009630 liquid culture Methods 0.000 description 6
• 239000000463 material Substances 0.000 description 6
• 230000036961 partial effect Effects 0.000 description 6
• 108091008146 restriction endonucleases Proteins 0.000 description 6
• UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 6
• 229960000268 spectinomycin Drugs 0.000 description 6
• 230000006641 stabilisation Effects 0.000 description 6
• 238000011105 stabilization Methods 0.000 description 6
• 229960005322 streptomycin Drugs 0.000 description 6
• 239000006228 supernatant Substances 0.000 description 6
• 108091035539 telomere Proteins 0.000 description 6
• 102000055501 telomere Human genes 0.000 description 6
• 244000105624 Arachis hypogaea Species 0.000 description 5
• 238000013459 approach Methods 0.000 description 5
• 210000004436 artificial bacterial chromosome Anatomy 0.000 description 5
• 238000004520 electroporation Methods 0.000 description 5
• 238000005286 illumination Methods 0.000 description 5
• BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 5
• 239000007788 liquid Substances 0.000 description 5
• 230000037353 metabolic pathway Effects 0.000 description 5
• 210000001938 protoplast Anatomy 0.000 description 5
• 210000003411 telomere Anatomy 0.000 description 5
• 210000001519 tissue Anatomy 0.000 description 5
• 108020004465 16S ribosomal RNA Proteins 0.000 description 4
• 102000005575 Cellulases Human genes 0.000 description 4
• 108010084185 Cellulases Proteins 0.000 description 4
• HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 4
• 244000064895 Cucumis melo subsp melo Species 0.000 description 4
• 235000010469 Glycine max Nutrition 0.000 description 4
• 244000068988 Glycine max Species 0.000 description 4
• 241000219823 Medicago Species 0.000 description 4
• 241001465754 Metazoa Species 0.000 description 4
• 235000010617 Phaseolus lunatus Nutrition 0.000 description 4
• 235000010627 Phaseolus vulgaris Nutrition 0.000 description 4
• 244000046052 Phaseolus vulgaris Species 0.000 description 4
• FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
• 240000006394 Sorghum bicolor Species 0.000 description 4
• 239000004098 Tetracycline Substances 0.000 description 4
• 239000007983 Tris buffer Substances 0.000 description 4
• ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 4
• 244000301083 Ustilago maydis Species 0.000 description 4
• 240000008042 Zea mays Species 0.000 description 4
• 239000002253 acid Substances 0.000 description 4
• 229960000723 ampicillin Drugs 0.000 description 4
• AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 4
• 230000003321 amplification Effects 0.000 description 4
• 210000001106 artificial yeast chromosome Anatomy 0.000 description 4
• 101150088806 atpA gene Proteins 0.000 description 4
• 230000001851 biosynthetic effect Effects 0.000 description 4
• 229930002875 chlorophyll Natural products 0.000 description 4
• 235000019804 chlorophyll Nutrition 0.000 description 4
• ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 4
• 210000000349 chromosome Anatomy 0.000 description 4
• 239000002299 complementary DNA Substances 0.000 description 4
• 230000029087 digestion Effects 0.000 description 4
• 244000013123 dwarf bean Species 0.000 description 4
• 238000001976 enzyme digestion Methods 0.000 description 4
• 238000002474 experimental method Methods 0.000 description 4
• 238000001502 gel electrophoresis Methods 0.000 description 4
• 230000002068 genetic effect Effects 0.000 description 4
• 239000001257 hydrogen Substances 0.000 description 4
• 229910052739 hydrogen Inorganic materials 0.000 description 4
• 238000002955 isolation Methods 0.000 description 4
• 230000000670 limiting effect Effects 0.000 description 4
• 238000003199 nucleic acid amplification method Methods 0.000 description 4
• 235000015097 nutrients Nutrition 0.000 description 4
• SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 4
• 238000002360 preparation method Methods 0.000 description 4
• 101150010007 psbD gene Proteins 0.000 description 4
• 101150074945 rbcL gene Proteins 0.000 description 4
• 238000012216 screening Methods 0.000 description 4
• 241000894007 species Species 0.000 description 4
• 230000004083 survival effect Effects 0.000 description 4
• 229960002180 tetracycline Drugs 0.000 description 4
• 229930101283 tetracycline Natural products 0.000 description 4
• 235000019364 tetracycline Nutrition 0.000 description 4
• 150000003522 tetracyclines Chemical class 0.000 description 4
• 238000013518 transcription Methods 0.000 description 4
• 230000035897 transcription Effects 0.000 description 4
• 238000013519 translation Methods 0.000 description 4
• LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 4
• XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
• 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
• QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
• 244000045232 Canavalia ensiformis Species 0.000 description 3
• 235000009467 Carica papaya Nutrition 0.000 description 3
• 240000006432 Carica papaya Species 0.000 description 3
• 241001147674 Chlorarachniophyceae Species 0.000 description 3
• 241000218631 Coniferophyta Species 0.000 description 3
• 240000001980 Cucurbita pepo Species 0.000 description 3
• 241000192700 Cyanobacteria Species 0.000 description 3
• 102000004594 DNA Polymerase I Human genes 0.000 description 3
• 108010017826 DNA Polymerase I Proteins 0.000 description 3
• 238000001712 DNA sequencing Methods 0.000 description 3
• 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 3
• 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 3
• WEEGYLXZBRQIMU-UHFFFAOYSA-N Eucalyptol Chemical compound C1CC2CCC1(C)OC2(C)C WEEGYLXZBRQIMU-UHFFFAOYSA-N 0.000 description 3
• 241000195623 Euglenida Species 0.000 description 3
• 241000233866 Fungi Species 0.000 description 3
• CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 3
• 229930182566 Gentamicin Natural products 0.000 description 3
• 244000299507 Gossypium hirsutum Species 0.000 description 3
• 241000206759 Haptophyceae Species 0.000 description 3
• FBOZXECLQNJBKD-ZDUSSCGKSA-N L-methotrexate Chemical compound C=1N=C2N=C(N)N=C(N)C2=NC=1CN(C)C1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 FBOZXECLQNJBKD-ZDUSSCGKSA-N 0.000 description 3
• QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 3
• 241000219739 Lens Species 0.000 description 3
• FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 3
• 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 3
• 229930193140 Neomycin Natural products 0.000 description 3
• 235000002637 Nicotiana tabacum Nutrition 0.000 description 3
• 244000061176 Nicotiana tabacum Species 0.000 description 3
• 240000007594 Oryza sativa Species 0.000 description 3
• 235000007164 Oryza sativa Nutrition 0.000 description 3
• 238000010222 PCR analysis Methods 0.000 description 3
• 235000008577 Pinus radiata Nutrition 0.000 description 3
• 241000218621 Pinus radiata Species 0.000 description 3
• 241000206572 Rhodophyta Species 0.000 description 3
• 235000002595 Solanum tuberosum Nutrition 0.000 description 3
• 244000061456 Solanum tuberosum Species 0.000 description 3
• 235000011684 Sorghum saccharatum Nutrition 0.000 description 3
• 238000002105 Southern blotting Methods 0.000 description 3
• 101150006914 TRP1 gene Proteins 0.000 description 3
• 108010022394 Threonine synthase Proteins 0.000 description 3
• 244000098338 Triticum aestivum Species 0.000 description 3
• LVTKHGUGBGNBPL-UHFFFAOYSA-N Trp-P-1 Chemical compound N1C2=CC=CC=C2C2=C1C(C)=C(N)N=C2C LVTKHGUGBGNBPL-UHFFFAOYSA-N 0.000 description 3
• QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 3
• 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
• 235000002017 Zea mays subsp mays Nutrition 0.000 description 3
• 229940088710 antibiotic agent Drugs 0.000 description 3
• 230000008238 biochemical pathway Effects 0.000 description 3
• 238000004113 cell culture Methods 0.000 description 3
• 230000000295 complement effect Effects 0.000 description 3
• 235000005822 corn Nutrition 0.000 description 3
• 102000004419 dihydrofolate reductase Human genes 0.000 description 3
• 235000005489 dwarf bean Nutrition 0.000 description 3
• 239000003623 enhancer Substances 0.000 description 3
• 210000003527 eukaryotic cell Anatomy 0.000 description 3
• 239000003502 gasoline Substances 0.000 description 3
• 239000001963 growth medium Substances 0.000 description 3
• 230000002363 herbicidal effect Effects 0.000 description 3
• 239000004009 herbicide Substances 0.000 description 3
• 125000005842 heteroatom Chemical group 0.000 description 3
• XMGQYMWWDOXHJM-UHFFFAOYSA-N limonene Natural products CC(=C)C1CCC(C)=CC1 XMGQYMWWDOXHJM-UHFFFAOYSA-N 0.000 description 3
• 239000012139 lysis buffer Substances 0.000 description 3
• 239000011777 magnesium Substances 0.000 description 3
• 229910052749 magnesium Inorganic materials 0.000 description 3
• 230000001404 mediated effect Effects 0.000 description 3
• 238000012269 metabolic engineering Methods 0.000 description 3
• 230000004060 metabolic process Effects 0.000 description 3
• 229960000485 methotrexate Drugs 0.000 description 3
• 229960004927 neomycin Drugs 0.000 description 3
• 229910052757 nitrogen Inorganic materials 0.000 description 3
• 210000000056 organ Anatomy 0.000 description 3
• 239000008188 pellet Substances 0.000 description 3
• 230000000644 propagated effect Effects 0.000 description 3
• 238000000746 purification Methods 0.000 description 3
• 239000011535 reaction buffer Substances 0.000 description 3
• 239000000758 substrate Substances 0.000 description 3
• 238000012360 testing method Methods 0.000 description 3
• 230000002103 transcriptional effect Effects 0.000 description 3
• 230000003612 virological effect Effects 0.000 description 3
• NDVASEGYNIMXJL-NXEZZACHSA-N (+)-sabinene Chemical compound C=C1CC[C@@]2(C(C)C)[C@@H]1C2 NDVASEGYNIMXJL-NXEZZACHSA-N 0.000 description 2
• 101710195549 (S)-beta-macrocarpene synthase Proteins 0.000 description 2
• SEHFUALWMUWDKS-UHFFFAOYSA-N 5-fluoroorotic acid Chemical compound OC(=O)C=1NC(=O)NC(=O)C=1F SEHFUALWMUWDKS-UHFFFAOYSA-N 0.000 description 2
• 235000007173 Abies balsamea Nutrition 0.000 description 2
• 244000283070 Abies balsamea Species 0.000 description 2
• 102000000452 Acetyl-CoA carboxylase Human genes 0.000 description 2
• 108010016219 Acetyl-CoA carboxylase Proteins 0.000 description 2
• 241000589155 Agrobacterium tumefaciens Species 0.000 description 2
• 240000007241 Agrostis stolonifera Species 0.000 description 2
• 244000144725 Amygdalus communis Species 0.000 description 2
• 235000011437 Amygdalus communis Nutrition 0.000 description 2
• 108010065511 Amylases Proteins 0.000 description 2
• 102000013142 Amylases Human genes 0.000 description 2
• 244000226021 Anacardium occidentale Species 0.000 description 2
• 244000099147 Ananas comosus Species 0.000 description 2
• 235000007119 Ananas comosus Nutrition 0.000 description 2
• 235000010777 Arachis hypogaea Nutrition 0.000 description 2
• 235000007319 Avena orientalis Nutrition 0.000 description 2
• 244000075850 Avena orientalis Species 0.000 description 2
• 235000014469 Bacillus subtilis Nutrition 0.000 description 2
• 241000335053 Beta vulgaris Species 0.000 description 2
• 108010018763 Biotin carboxylase Proteins 0.000 description 2
• 235000011331 Brassica Nutrition 0.000 description 2
• 241000219198 Brassica Species 0.000 description 2
• 240000002791 Brassica napus Species 0.000 description 2
• 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 2
• 240000003259 Brassica oleracea var. botrytis Species 0.000 description 2
• 241000195940 Bryophyta Species 0.000 description 2
• UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 2
• 241001674345 Callitropsis nootkatensis Species 0.000 description 2
• 244000025254 Cannabis sativa Species 0.000 description 2
• 235000003255 Carthamus tinctorius Nutrition 0.000 description 2
• 244000020518 Carthamus tinctorius Species 0.000 description 2
• 235000010523 Cicer arietinum Nutrition 0.000 description 2
• 244000045195 Cicer arietinum Species 0.000 description 2
• 240000006740 Cichorium endivia Species 0.000 description 2
• 244000298479 Cichorium intybus Species 0.000 description 2
• 241000207199 Citrus Species 0.000 description 2
• 241001672694 Citrus reticulata Species 0.000 description 2
• 235000013162 Cocos nucifera Nutrition 0.000 description 2
• 244000060011 Cocos nucifera Species 0.000 description 2
• 229920000742 Cotton Polymers 0.000 description 2
• 235000009847 Cucumis melo var cantalupensis Nutrition 0.000 description 2
• 240000008067 Cucumis sativus Species 0.000 description 2
• 235000009854 Cucurbita moschata Nutrition 0.000 description 2
• 235000009852 Cucurbita pepo Nutrition 0.000 description 2
• 235000009355 Dianthus caryophyllus Nutrition 0.000 description 2
• 240000006497 Dianthus caryophyllus Species 0.000 description 2
• YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 2
• 240000002395 Euphorbia pulcherrima Species 0.000 description 2
• WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
• 239000005562 Glyphosate Substances 0.000 description 2
• 244000020551 Helianthus annuus Species 0.000 description 2
• 235000003222 Helianthus annuus Nutrition 0.000 description 2
• 241000238631 Hexapoda Species 0.000 description 2
• 235000005206 Hibiscus Nutrition 0.000 description 2
• 235000007185 Hibiscus lunariifolius Nutrition 0.000 description 2
• 244000284380 Hibiscus rosa sinensis Species 0.000 description 2
• 235000007340 Hordeum vulgare Nutrition 0.000 description 2
• 240000005979 Hordeum vulgare Species 0.000 description 2
• 244000267823 Hydrangea macrophylla Species 0.000 description 2
• 235000014486 Hydrangea macrophylla Nutrition 0.000 description 2
• UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 2
• SIKJAQJRHWYJAI-UHFFFAOYSA-N Indole Chemical compound C1=CC=C2NC=CC2=C1 SIKJAQJRHWYJAI-UHFFFAOYSA-N 0.000 description 2
• RRHGJUQNOFWUDK-UHFFFAOYSA-N Isoprene Chemical group CC(=C)C=C RRHGJUQNOFWUDK-UHFFFAOYSA-N 0.000 description 2
• KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 2
• HNDVDQJCIGZPNO-YFKPBYRVSA-N L-histidine Chemical compound OC(=O)[C@@H](N)CC1=CN=CN1 HNDVDQJCIGZPNO-YFKPBYRVSA-N 0.000 description 2
• 235000003228 Lactuca sativa Nutrition 0.000 description 2
• 240000008415 Lactuca sativa Species 0.000 description 2
• 108091026898 Leader sequence (mRNA) Proteins 0.000 description 2
• 235000014647 Lens culinaris subsp culinaris Nutrition 0.000 description 2
• 241000219745 Lupinus Species 0.000 description 2
• 235000007688 Lycopersicon esculentum Nutrition 0.000 description 2
• TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 2
• 241000218922 Magnoliophyta Species 0.000 description 2
• 235000014826 Mangifera indica Nutrition 0.000 description 2
• 240000007228 Mangifera indica Species 0.000 description 2
• 108020005196 Mitochondrial DNA Proteins 0.000 description 2
• 240000005561 Musa balbisiana Species 0.000 description 2
• 241000234479 Narcissus Species 0.000 description 2
• 240000007817 Olea europaea Species 0.000 description 2
• 108700026244 Open Reading Frames Proteins 0.000 description 2
• 235000007199 Panicum miliaceum Nutrition 0.000 description 2
• 235000007195 Pennisetum typhoides Nutrition 0.000 description 2
• 240000007377 Petunia x hybrida Species 0.000 description 2
• ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 2
• IAJOBQBIJHVGMQ-UHFFFAOYSA-N Phosphinothricin Natural products CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 2
• 235000008331 Pinus X rigitaeda Nutrition 0.000 description 2
• 241000018646 Pinus brutia Species 0.000 description 2
• 235000011613 Pinus brutia Nutrition 0.000 description 2
• 241000218606 Pinus contorta Species 0.000 description 2
• 235000013267 Pinus ponderosa Nutrition 0.000 description 2
• 235000008566 Pinus taeda Nutrition 0.000 description 2
• 241000218679 Pinus taeda Species 0.000 description 2
• 235000010582 Pisum sativum Nutrition 0.000 description 2
• 240000004713 Pisum sativum Species 0.000 description 2
• 108010059820 Polygalacturonase Proteins 0.000 description 2
• 241000219000 Populus Species 0.000 description 2
• ATUOYWHBWRKTHZ-UHFFFAOYSA-N Propane Chemical compound CCC ATUOYWHBWRKTHZ-UHFFFAOYSA-N 0.000 description 2
• 240000001416 Pseudotsuga menziesii Species 0.000 description 2
• 108020004511 Recombinant DNA Proteins 0.000 description 2
• 241000208422 Rhododendron Species 0.000 description 2
• 108010083644 Ribonucleases Proteins 0.000 description 2
• 102000006382 Ribonucleases Human genes 0.000 description 2
• 235000007238 Secale cereale Nutrition 0.000 description 2
• 244000082988 Secale cereale Species 0.000 description 2
• 240000005498 Setaria italica Species 0.000 description 2
• VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
• 240000003768 Solanum lycopersicum Species 0.000 description 2
• 244000062793 Sorghum vulgare Species 0.000 description 2
• 229940100389 Sulfonylurea Drugs 0.000 description 2
• 241000223892 Tetrahymena Species 0.000 description 2
• 244000269722 Thea sinensis Species 0.000 description 2
• 244000299461 Theobroma cacao Species 0.000 description 2
• 235000009470 Theobroma cacao Nutrition 0.000 description 2
• 102000005488 Thioesterase Human genes 0.000 description 2
• 108091036066 Three prime untranslated region Proteins 0.000 description 2
• 241000592342 Tracheophyta Species 0.000 description 2
• 241000219793 Trifolium Species 0.000 description 2
• 235000021307 Triticum Nutrition 0.000 description 2
• 240000003021 Tsuga heterophylla Species 0.000 description 2
• 244000078534 Vaccinium myrtillus Species 0.000 description 2
• 235000010749 Vicia faba Nutrition 0.000 description 2
• 240000006677 Vicia faba Species 0.000 description 2
• 235000002098 Vicia faba var. major Nutrition 0.000 description 2
• 241000219977 Vigna Species 0.000 description 2
• 240000004922 Vigna radiata Species 0.000 description 2
• 235000010721 Vigna radiata var radiata Nutrition 0.000 description 2
• 235000011469 Vigna radiata var sublobata Nutrition 0.000 description 2
• 235000010726 Vigna sinensis Nutrition 0.000 description 2
• 241000588902 Zymomonas mobilis Species 0.000 description 2
• 230000001133 acceleration Effects 0.000 description 2
• 238000009825 accumulation Methods 0.000 description 2
• 150000001335 aliphatic alkanes Chemical class 0.000 description 2
• ANVAOWXLWRTKGA-XHGAXZNDSA-N all-trans-alpha-carotene Chemical compound CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1C(C)=CCCC1(C)C ANVAOWXLWRTKGA-XHGAXZNDSA-N 0.000 description 2
• 150000001413 amino acids Chemical class 0.000 description 2
• 235000019418 amylase Nutrition 0.000 description 2
• 229940025131 amylases Drugs 0.000 description 2
• 230000001195 anabolic effect Effects 0.000 description 2
• 210000004507 artificial chromosome Anatomy 0.000 description 2
• 239000010426 asphalt Substances 0.000 description 2
• 125000004429 atom Chemical group 0.000 description 2
• UAHWPYUMFXYFJY-UHFFFAOYSA-N beta-myrcene Chemical compound CC(C)=CCCC(=C)C=C UAHWPYUMFXYFJY-UHFFFAOYSA-N 0.000 description 2
• 230000006696 biosynthetic metabolic pathway Effects 0.000 description 2
• 230000015572 biosynthetic process Effects 0.000 description 2
• 244000022203 blackseeded proso millet Species 0.000 description 2
• 239000000872 buffer Substances 0.000 description 2
• 239000001110 calcium chloride Substances 0.000 description 2
• 229910001628 calcium chloride Inorganic materials 0.000 description 2
• 239000001506 calcium phosphate Substances 0.000 description 2
• 229910000389 calcium phosphate Inorganic materials 0.000 description 2
• 235000011010 calcium phosphates Nutrition 0.000 description 2
• CRPUJAZIXJMDBK-UHFFFAOYSA-N camphene Chemical compound C1CC2C(=C)C(C)(C)C1C2 CRPUJAZIXJMDBK-UHFFFAOYSA-N 0.000 description 2
• 125000004432 carbon atom Chemical group C* 0.000 description 2
• 235000021466 carotenoid Nutrition 0.000 description 2
• 150000001747 carotenoids Chemical class 0.000 description 2
• 239000003153 chemical reaction reagent Substances 0.000 description 2
• 239000003795 chemical substances by application Substances 0.000 description 2
• 235000003733 chicria Nutrition 0.000 description 2
• 229960005091 chloramphenicol Drugs 0.000 description 2
• WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 2
• 235000020971 citrus fruits Nutrition 0.000 description 2
• 239000000571 coke Substances 0.000 description 2
• 238000010276 construction Methods 0.000 description 2
• 239000010779 crude oil Substances 0.000 description 2
• 238000005520 cutting process Methods 0.000 description 2
• 150000001924 cycloalkanes Chemical class 0.000 description 2
• 230000009089 cytolysis Effects 0.000 description 2
• 235000013399 edible fruits Nutrition 0.000 description 2
• 230000005284 excitation Effects 0.000 description 2
• 239000013604 expression vector Substances 0.000 description 2
• 230000004136 fatty acid synthesis Effects 0.000 description 2
• ODKNJVUHOIMIIZ-RRKCRQDMSA-N floxuridine Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(F)=C1 ODKNJVUHOIMIIZ-RRKCRQDMSA-N 0.000 description 2
• 229960000961 floxuridine Drugs 0.000 description 2
• 239000007789 gas Substances 0.000 description 2
• 239000000499 gel Substances 0.000 description 2
• 239000008103 glucose Substances 0.000 description 2
• XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 2
• 229940097068 glyphosate Drugs 0.000 description 2
• PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
• 229910052737 gold Inorganic materials 0.000 description 2
• 239000010931 gold Substances 0.000 description 2
• HNDVDQJCIGZPNO-UHFFFAOYSA-N histidine Natural products OC(=O)C(N)CC1=CN=CN1 HNDVDQJCIGZPNO-UHFFFAOYSA-N 0.000 description 2
• 238000009396 hybridization Methods 0.000 description 2
• 230000001939 inductive effect Effects 0.000 description 2
• 230000000977 initiatory effect Effects 0.000 description 2
• 239000003350 kerosene Substances 0.000 description 2
• 230000001533 ligninolytic effect Effects 0.000 description 2
• 235000001510 limonene Nutrition 0.000 description 2
• 229940087305 limonene Drugs 0.000 description 2
• 150000002632 lipids Chemical class 0.000 description 2
• 239000010687 lubricating oil Substances 0.000 description 2
• 101150109301 lys2 gene Proteins 0.000 description 2
• 230000002934 lysing effect Effects 0.000 description 2
• 230000002101 lytic effect Effects 0.000 description 2
• 210000004962 mammalian cell Anatomy 0.000 description 2
• 238000013507 mapping Methods 0.000 description 2
• 229910052751 metal Inorganic materials 0.000 description 2
• 239000002184 metal Substances 0.000 description 2
• VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
• 238000000520 microinjection Methods 0.000 description 2
• 235000019713 millet Nutrition 0.000 description 2
• 230000003472 neutralizing effect Effects 0.000 description 2
• 239000003921 oil Substances 0.000 description 2
• 235000019198 oils Nutrition 0.000 description 2
• 125000001477 organic nitrogen group Chemical group 0.000 description 2
• 235000020232 peanut Nutrition 0.000 description 2
• 239000003348 petrochemical agent Substances 0.000 description 2
• 239000003208 petroleum Substances 0.000 description 2
• 239000003209 petroleum derivative Substances 0.000 description 2
• 239000004033 plastic Substances 0.000 description 2
• 229920003023 plastic Polymers 0.000 description 2
• 235000011056 potassium acetate Nutrition 0.000 description 2
• 238000012545 processing Methods 0.000 description 2
• 210000001236 prokaryotic cell Anatomy 0.000 description 2
• 238000003906 pulsed field gel electrophoresis Methods 0.000 description 2
• 230000002285 radioactive effect Effects 0.000 description 2
• 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 2
• 235000009566 rice Nutrition 0.000 description 2
• 238000007363 ring formation reaction Methods 0.000 description 2
• 229940016590 sarkosyl Drugs 0.000 description 2
• 108700004121 sarkosyl Proteins 0.000 description 2
• 229920006395 saturated elastomer Polymers 0.000 description 2
• 239000001632 sodium acetate Substances 0.000 description 2
• 235000017281 sodium acetate Nutrition 0.000 description 2
• 239000011780 sodium chloride Substances 0.000 description 2
• KSAVQLQVUXSOCR-UHFFFAOYSA-M sodium lauroyl sarcosinate Chemical compound [Na+].CCCCCCCCCCCC(=O)N(C)CC([O-])=O KSAVQLQVUXSOCR-UHFFFAOYSA-M 0.000 description 2
• ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 description 2
• 238000010561 standard procedure Methods 0.000 description 2
• 239000000126 substance Substances 0.000 description 2
• YROXIXLRRCOBKF-UHFFFAOYSA-N sulfonylurea Chemical class OC(=N)N=S(=O)=O YROXIXLRRCOBKF-UHFFFAOYSA-N 0.000 description 2
• 230000008093 supporting effect Effects 0.000 description 2
• 239000011269 tar Substances 0.000 description 2
• 108020002982 thioesterase Proteins 0.000 description 2
• 238000001890 transfection Methods 0.000 description 2
• 238000011426 transformation method Methods 0.000 description 2
• QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 2
• WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 2
• 229910052721 tungsten Inorganic materials 0.000 description 2
• 239000010937 tungsten Substances 0.000 description 2
• 229940035893 uracil Drugs 0.000 description 2
• 239000001993 wax Substances 0.000 description 2
• 108010082737 zymolyase Proteins 0.000 description 2
• BQOFWKZOCNGFEC-BDAKNGLRSA-N (+)-Delta3-carene Chemical compound C1C(C)=CC[C@H]2C(C)(C)[C@@H]12 BQOFWKZOCNGFEC-BDAKNGLRSA-N 0.000 description 1
• 108030001864 (+)-bornyl diphosphate synthases Proteins 0.000 description 1
• 229930006713 (+)-car-3-ene Natural products 0.000 description 1
• BBPXZLJCPUPNGH-CMKODMSKSA-N (-)-Abietadiene Chemical compound CC1(C)CCC[C@]2(C)[C@@H](CCC(C(C)C)=C3)C3=CC[C@H]21 BBPXZLJCPUPNGH-CMKODMSKSA-N 0.000 description 1
• GRWFGVWFFZKLTI-IUCAKERBSA-N (-)-α-pinene Chemical compound CC1=CC[C@@H]2C(C)(C)[C@H]1C2 GRWFGVWFFZKLTI-IUCAKERBSA-N 0.000 description 1
• FAMPSKZZVDUYOS-HRGUGZIWSA-N (1E,4E,8E)-alpha-humulene Chemical compound C\C1=C/CC(C)(C)\C=C\C\C(C)=C\CC1 FAMPSKZZVDUYOS-HRGUGZIWSA-N 0.000 description 1
• CXNPLSGKWMLZPZ-GIFSMMMISA-N (2r,3r,6s)-3-[[(3s)-3-amino-5-[carbamimidoyl(methyl)amino]pentanoyl]amino]-6-(4-amino-2-oxopyrimidin-1-yl)-3,6-dihydro-2h-pyran-2-carboxylic acid Chemical compound O1[C@@H](C(O)=O)[C@H](NC(=O)C[C@@H](N)CCN(C)C(N)=N)C=C[C@H]1N1C(=O)N=C(N)C=C1 CXNPLSGKWMLZPZ-GIFSMMMISA-N 0.000 description 1
• XBGUIVFBMBVUEG-PFONDFGASA-N (E)-gamma-bisabolene Chemical compound CC(C)=CCC\C(C)=C1/CCC(C)=CC1 XBGUIVFBMBVUEG-PFONDFGASA-N 0.000 description 1
• WUOACPNHFRMFPN-SECBINFHSA-N (S)-(-)-alpha-terpineol Chemical compound CC1=CC[C@@H](C(C)(C)O)CC1 WUOACPNHFRMFPN-SECBINFHSA-N 0.000 description 1
• 239000001169 1-methyl-4-propan-2-ylcyclohexa-1,4-diene Substances 0.000 description 1
• ONVABDHFQKWOSV-UHFFFAOYSA-N 16-Phyllocladene Natural products C1CC(C2)C(=C)CC32CCC2C(C)(C)CCCC2(C)C31 ONVABDHFQKWOSV-UHFFFAOYSA-N 0.000 description 1
• QRBLKGHRWFGINE-UGWAGOLRSA-N 2-[2-[2-[[2-[[4-[[2-[[6-amino-2-[3-amino-1-[(2,3-diamino-3-oxopropyl)amino]-3-oxopropyl]-5-methylpyrimidine-4-carbonyl]amino]-3-[(2r,3s,4s,5s,6s)-3-[(2s,3r,4r,5s)-4-carbamoyl-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-4,5-dihydroxy-6-(hydroxymethyl)- Chemical compound N=1C(C=2SC=C(N=2)C(N)=O)CSC=1CCNC(=O)C(C(C)=O)NC(=O)C(C)C(O)C(C)NC(=O)C(C(O[C@H]1[C@@]([C@@H](O)[C@H](O)[C@H](CO)O1)(C)O[C@H]1[C@@H]([C@](O)([C@@H](O)C(CO)O1)C(N)=O)O)C=1NC=NC=1)NC(=O)C1=NC(C(CC(N)=O)NCC(N)C(N)=O)=NC(N)=C1C QRBLKGHRWFGINE-UGWAGOLRSA-N 0.000 description 1
• VWFJDQUYCIWHTN-YFVJMOTDSA-N 2-trans,6-trans-farnesyl diphosphate Chemical compound CC(C)=CCC\C(C)=C\CC\C(C)=C\CO[P@](O)(=O)OP(O)(O)=O VWFJDQUYCIWHTN-YFVJMOTDSA-N 0.000 description 1
• IVBZYUKCNLJUDA-UHFFFAOYSA-N 3,3a,6-trimethyl-1-(propan-2-yl)-2,3,3a,4-tetrahydro-1h-indene Chemical compound C1C=C(C)C=C2C(C(C)C)CC(C)C21C IVBZYUKCNLJUDA-UHFFFAOYSA-N 0.000 description 1
• 101710186512 3-ketoacyl-CoA thiolase Proteins 0.000 description 1
• 108010014293 5-epi-aristolochene synthase Proteins 0.000 description 1
• JCAIWDXKLCEQEO-PGHZQYBFSA-N 5beta,9alpha,10alpha-labda-8(20),13-dien-15-yl diphosphate Chemical compound CC1(C)CCC[C@@]2(C)[C@H](CCC(/C)=C/COP(O)(=O)OP(O)(O)=O)C(=C)CC[C@@H]21 JCAIWDXKLCEQEO-PGHZQYBFSA-N 0.000 description 1
• 108010000951 8-epicedrol synthase Proteins 0.000 description 1
• 240000004507 Abelmoschus esculentus Species 0.000 description 1
• 235000004507 Abies alba Nutrition 0.000 description 1
• 235000014081 Abies amabilis Nutrition 0.000 description 1
• 244000101408 Abies amabilis Species 0.000 description 1
• 244000178606 Abies grandis Species 0.000 description 1
• 235000017894 Abies grandis Nutrition 0.000 description 1
• 235000004710 Abies lasiocarpa Nutrition 0.000 description 1
• BBPXZLJCPUPNGH-UHFFFAOYSA-N Abietadien Natural products CC1(C)CCCC2(C)C(CCC(C(C)C)=C3)C3=CCC21 BBPXZLJCPUPNGH-UHFFFAOYSA-N 0.000 description 1
• 240000005020 Acaciella glauca Species 0.000 description 1
• 108010000700 Acetolactate synthase Proteins 0.000 description 1
• 235000009436 Actinidia deliciosa Nutrition 0.000 description 1
• 244000298697 Actinidia deliciosa Species 0.000 description 1
• 241000186361 Actinobacteria <class> Species 0.000 description 1
• 102000007469 Actins Human genes 0.000 description 1
• 108010085238 Actins Proteins 0.000 description 1
• 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
• 229920000936 Agarose Polymers 0.000 description 1
• 241000589158 Agrobacterium Species 0.000 description 1
• 241000234282 Allium Species 0.000 description 1
• 235000005254 Allium ampeloprasum Nutrition 0.000 description 1
• 240000006108 Allium ampeloprasum Species 0.000 description 1
• 235000002732 Allium cepa var. cepa Nutrition 0.000 description 1
• 240000002234 Allium sativum Species 0.000 description 1
• 241000219318 Amaranthus Species 0.000 description 1
• 235000004047 Amorpha fruticosa Nutrition 0.000 description 1
• 240000002066 Amorpha fruticosa Species 0.000 description 1
• 244000144730 Amygdalus persica Species 0.000 description 1
• 235000001274 Anacardium occidentale Nutrition 0.000 description 1
• 235000003276 Apios tuberosa Nutrition 0.000 description 1
• 240000007087 Apium graveolens Species 0.000 description 1
• 235000015849 Apium graveolens Dulce Group Nutrition 0.000 description 1
• 235000010591 Appio Nutrition 0.000 description 1
• 241000218156 Aquilegia Species 0.000 description 1
• 101000906787 Arabidopsis thaliana 1,8-cineole synthase 1, chloroplastic Proteins 0.000 description 1
• 101000906782 Arabidopsis thaliana 1,8-cineole synthase 2, chloroplastic Proteins 0.000 description 1
• 101100163849 Arabidopsis thaliana ARS1 gene Proteins 0.000 description 1
• 235000003911 Arachis Nutrition 0.000 description 1
• 235000010744 Arachis villosulicarpa Nutrition 0.000 description 1
• 241000203069 Archaea Species 0.000 description 1
• 102100024630 Asc-type amino acid transporter 1 Human genes 0.000 description 1
• 101001065065 Aspergillus awamori Feruloyl esterase A Proteins 0.000 description 1
• 241001465318 Aspergillus terreus Species 0.000 description 1
• 235000007558 Avena sp Nutrition 0.000 description 1
• 235000000832 Ayote Nutrition 0.000 description 1
• 244000063299 Bacillus subtilis Species 0.000 description 1
• 241000218993 Begonia Species 0.000 description 1
• 235000016068 Berberis vulgaris Nutrition 0.000 description 1
• 235000021533 Beta vulgaris Nutrition 0.000 description 1
• 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 1
• 108010006654 Bleomycin Proteins 0.000 description 1
• 108030000358 Botryococcene synthases Proteins 0.000 description 1
• 244000178993 Brassica juncea Species 0.000 description 1
• 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 1
• 235000011293 Brassica napus Nutrition 0.000 description 1
• 235000006008 Brassica napus var napus Nutrition 0.000 description 1
• 240000000385 Brassica napus var. napus Species 0.000 description 1
• 240000007124 Brassica oleracea Species 0.000 description 1
• 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
• 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
• 235000017647 Brassica oleracea var italica Nutrition 0.000 description 1
• 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
• 240000008100 Brassica rapa Species 0.000 description 1
• 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 1
• 235000000540 Brassica rapa subsp rapa Nutrition 0.000 description 1
• 235000004977 Brassica sinapistrum Nutrition 0.000 description 1
• 241000220243 Brassica sp. Species 0.000 description 1
• 235000004936 Bromus mango Nutrition 0.000 description 1
• BOJWREQWHOWAEL-UHFFFAOYSA-N C=C1CCSCC1 Chemical compound C=C1CCSCC1 BOJWREQWHOWAEL-UHFFFAOYSA-N 0.000 description 1
• 101100307034 Caenorhabditis elegans rps-12 gene Proteins 0.000 description 1
• 235000012766 Cannabis sativa ssp. sativa var. sativa Nutrition 0.000 description 1
• 235000012765 Cannabis sativa ssp. sativa var. spontanea Nutrition 0.000 description 1
• 235000002566 Capsicum Nutrition 0.000 description 1
• 241000218645 Cedrus Species 0.000 description 1
• 235000013912 Ceratonia siliqua Nutrition 0.000 description 1
• 240000008886 Ceratonia siliqua Species 0.000 description 1
• 241000219312 Chenopodium Species 0.000 description 1
• 241000195585 Chlamydomonas Species 0.000 description 1
• 235000007516 Chrysanthemum Nutrition 0.000 description 1
• 244000189548 Chrysanthemum x morifolium Species 0.000 description 1
• 235000007542 Cichorium intybus Nutrition 0.000 description 1
• 241001633993 Cineraria Species 0.000 description 1
• 108020004638 Circular DNA Proteins 0.000 description 1
• 108030003505 Cis-muuroladiene synthases Proteins 0.000 description 1
• 244000241235 Citrullus lanatus Species 0.000 description 1
• 235000012828 Citrullus lanatus var citroides Nutrition 0.000 description 1
• 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
• 235000005979 Citrus limon Nutrition 0.000 description 1
• 244000131522 Citrus pyriformis Species 0.000 description 1
• 240000000560 Citrus x paradisi Species 0.000 description 1
• 101710118490 Copalyl diphosphate synthase Proteins 0.000 description 1
• 244000018436 Coriandrum sativum Species 0.000 description 1
• 241000522193 Coronilla Species 0.000 description 1
• 235000004035 Cryptotaenia japonica Nutrition 0.000 description 1
• 241000219112 Cucumis Species 0.000 description 1
• 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
• 235000015001 Cucumis melo var inodorus Nutrition 0.000 description 1
• 240000002495 Cucumis melo var. inodorus Species 0.000 description 1
• 235000010071 Cucumis prophetarum Nutrition 0.000 description 1
• 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
• 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 1
• 241000219130 Cucurbita pepo subsp. pepo Species 0.000 description 1
• 235000003954 Cucurbita pepo var melopepo Nutrition 0.000 description 1
• 244000007835 Cyamopsis tetragonoloba Species 0.000 description 1
• 241000612153 Cyclamen Species 0.000 description 1
• 235000017788 Cydonia oblonga Nutrition 0.000 description 1
• 244000019459 Cynara cardunculus Species 0.000 description 1
• 235000019106 Cynara scolymus Nutrition 0.000 description 1
• FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 description 1
• IGXWBGJHJZYPQS-SSDOTTSWSA-N D-Luciferin Chemical compound OC(=O)[C@H]1CSC(C=2SC3=CC=C(O)C=C3N=2)=N1 IGXWBGJHJZYPQS-SSDOTTSWSA-N 0.000 description 1
• WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
• 230000004544 DNA amplification Effects 0.000 description 1
• 240000004585 Dactylis glomerata Species 0.000 description 1
• 235000012040 Dahlia pinnata Nutrition 0.000 description 1
• 244000033273 Dahlia variabilis Species 0.000 description 1
• 241000208296 Datura Species 0.000 description 1
• 235000002767 Daucus carota Nutrition 0.000 description 1
• 244000000626 Daucus carota Species 0.000 description 1
• CYCGRDQQIOGCKX-UHFFFAOYSA-N Dehydro-luciferin Natural products OC(=O)C1=CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 CYCGRDQQIOGCKX-UHFFFAOYSA-N 0.000 description 1
• 241000202296 Delphinium Species 0.000 description 1
• BQOFWKZOCNGFEC-UHFFFAOYSA-N Delta3-Carene Natural products C1C(C)=CCC2C(C)(C)C12 BQOFWKZOCNGFEC-UHFFFAOYSA-N 0.000 description 1
• 229920002307 Dextran Polymers 0.000 description 1
• 235000011511 Diospyros Nutrition 0.000 description 1
• 244000236655 Diospyros kaki Species 0.000 description 1
• 235000014466 Douglas bleu Nutrition 0.000 description 1
• 235000007349 Eleusine coracana Nutrition 0.000 description 1
• 244000078127 Eleusine coracana Species 0.000 description 1
• 108010067770 Endopeptidase K Proteins 0.000 description 1
• 108030004983 Epi-cedrol synthases Proteins 0.000 description 1
• XVULBTBTFGYVRC-UHFFFAOYSA-N Episclareol Natural products CC1(C)CCCC2(C)C(CCC(O)(C)C=C)C(C)(O)CCC21 XVULBTBTFGYVRC-UHFFFAOYSA-N 0.000 description 1
• 244000024675 Eruca sativa Species 0.000 description 1
• 235000014755 Eruca sativa Nutrition 0.000 description 1
• OTMSDBZUPAUEDD-UHFFFAOYSA-N Ethane Chemical compound CC OTMSDBZUPAUEDD-UHFFFAOYSA-N 0.000 description 1
• 244000004281 Eucalyptus maculata Species 0.000 description 1
• 241000206602 Eukaryota Species 0.000 description 1
• 108091029865 Exogenous DNA Proteins 0.000 description 1
• 108700024394 Exon Proteins 0.000 description 1
• 235000009419 Fagopyrum esculentum Nutrition 0.000 description 1
• 240000008620 Fagopyrum esculentum Species 0.000 description 1
• VWFJDQUYCIWHTN-UHFFFAOYSA-N Farnesyl pyrophosphate Natural products CC(C)=CCCC(C)=CCCC(C)=CCOP(O)(=O)OP(O)(O)=O VWFJDQUYCIWHTN-UHFFFAOYSA-N 0.000 description 1
• 241000234643 Festuca arundinacea Species 0.000 description 1
• 241000218218 Ficus <angiosperm> Species 0.000 description 1
• BJGNCJDXODQBOB-UHFFFAOYSA-N Fivefly Luciferin Natural products OC(=O)C1CSC(C=2SC3=CC(O)=CC=C3N=2)=N1 BJGNCJDXODQBOB-UHFFFAOYSA-N 0.000 description 1
• 240000006927 Foeniculum vulgare Species 0.000 description 1
• 235000004204 Foeniculum vulgare Nutrition 0.000 description 1
• 235000016623 Fragaria vesca Nutrition 0.000 description 1
• 240000009088 Fragaria x ananassa Species 0.000 description 1
• 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
• 241000287828 Gallus gallus Species 0.000 description 1
• 241000204888 Geobacter sp. Species 0.000 description 1
• 241000735332 Gerbera Species 0.000 description 1
• 108010048467 Germacrene C synthase Proteins 0.000 description 1
• 108030004951 Germacrene-A synthases Proteins 0.000 description 1
• 241000245654 Gladiolus Species 0.000 description 1
• 240000005322 Gloxinia perennis Species 0.000 description 1
• 239000005561 Glufosinate Substances 0.000 description 1
• 240000000047 Gossypium barbadense Species 0.000 description 1
• 235000009429 Gossypium barbadense Nutrition 0.000 description 1
• 235000009432 Gossypium hirsutum Nutrition 0.000 description 1
• 108010043121 Green Fluorescent Proteins Proteins 0.000 description 1
• 102000004144 Green Fluorescent Proteins Human genes 0.000 description 1
• 241000168525 Haematococcus Species 0.000 description 1
• 229920002488 Hemicellulose Polymers 0.000 description 1
• 241000234473 Hippeastrum Species 0.000 description 1
• 241000282412 Homo Species 0.000 description 1
• 241000701024 Human betaherpesvirus 5 Species 0.000 description 1
• 108090001042 Hydro-Lyases Proteins 0.000 description 1
• 102000004867 Hydro-Lyases Human genes 0.000 description 1
• 206010020649 Hyperkeratosis Diseases 0.000 description 1
• 241001495448 Impatiens <genus> Species 0.000 description 1
• 102000015696 Interleukins Human genes 0.000 description 1
• 108010063738 Interleukins Proteins 0.000 description 1
• 235000021506 Ipomoea Nutrition 0.000 description 1
• 241000207783 Ipomoea Species 0.000 description 1
• 235000002678 Ipomoea batatas Nutrition 0.000 description 1
• 244000017020 Ipomoea batatas Species 0.000 description 1
• 101710183158 Isopimaradiene synthase Proteins 0.000 description 1
• 241000721662 Juniperus Species 0.000 description 1
• 235000014556 Juniperus scopulorum Nutrition 0.000 description 1
• 235000014560 Juniperus virginiana var silicicola Nutrition 0.000 description 1
• 108010025815 Kanamycin Kinase Proteins 0.000 description 1
• ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
• 101150007280 LEU2 gene Proteins 0.000 description 1
• 241000219729 Lathyrus Species 0.000 description 1
• 241000209499 Lemna Species 0.000 description 1
• 244000207740 Lemna minor Species 0.000 description 1
• 235000006439 Lemna minor Nutrition 0.000 description 1
• 101001110310 Lentilactobacillus kefiri NADP-dependent (R)-specific alcohol dehydrogenase Proteins 0.000 description 1
• 235000010643 Leucaena leucocephala Nutrition 0.000 description 1
• 240000007472 Leucaena leucocephala Species 0.000 description 1
• ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
• 108090000364 Ligases Proteins 0.000 description 1
• 102000003960 Ligases Human genes 0.000 description 1
• 108010054320 Lignin peroxidase Proteins 0.000 description 1
• 108090001060 Lipase Proteins 0.000 description 1
• 102000004882 Lipase Human genes 0.000 description 1
• 239000004367 Lipase Substances 0.000 description 1
• 241000208682 Liquidambar Species 0.000 description 1
• 235000006552 Liquidambar styraciflua Nutrition 0.000 description 1
• WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
• 240000004296 Lolium perenne Species 0.000 description 1
• 108030004940 Longifolene synthases Proteins 0.000 description 1
• 108060001084 Luciferase Proteins 0.000 description 1
• 239000005089 Luciferase Substances 0.000 description 1
• DDWFXDSYGUXRAY-UHFFFAOYSA-N Luciferin Natural products CCc1c(C)c(CC2NC(=O)C(=C2C=C)C)[nH]c1Cc3[nH]c4C(=C5/NC(CC(=O)O)C(C)C5CC(=O)O)CC(=O)c4c3C DDWFXDSYGUXRAY-UHFFFAOYSA-N 0.000 description 1
• 239000006142 Luria-Bertani Agar Substances 0.000 description 1
• 239000006137 Luria-Bertani broth Substances 0.000 description 1
• UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 1
• JEVVKJMRZMXFBT-XWDZUXABSA-N Lycophyll Natural products OC/C(=C/CC/C(=C\C=C\C(=C/C=C/C(=C\C=C\C=C(/C=C/C=C(\C=C\C=C(/CC/C=C(/CO)\C)\C)/C)\C)/C)\C)/C)/C JEVVKJMRZMXFBT-XWDZUXABSA-N 0.000 description 1
• 241000208467 Macadamia Species 0.000 description 1
• 235000018330 Macadamia integrifolia Nutrition 0.000 description 1
• 240000007575 Macadamia integrifolia Species 0.000 description 1
• 235000011430 Malus pumila Nutrition 0.000 description 1
• 235000015103 Malus silvestris Nutrition 0.000 description 1
• 108010059896 Manganese peroxidase Proteins 0.000 description 1
• 240000003183 Manihot esculenta Species 0.000 description 1
• 235000004456 Manihot esculenta Nutrition 0.000 description 1
• 108091022912 Mannose-6-Phosphate Isomerase Proteins 0.000 description 1
• 240000004658 Medicago sativa Species 0.000 description 1
• 235000010624 Medicago sativa Nutrition 0.000 description 1
• 241000213996 Melilotus Species 0.000 description 1
• 235000009072 Mesembryanthemum Nutrition 0.000 description 1
• 241000219480 Mesembryanthemum Species 0.000 description 1
• 101100261636 Methanothermobacter marburgensis (strain ATCC BAA-927 / DSM 2133 / JCM 14651 / NBRC 100331 / OCM 82 / Marburg) trpB2 gene Proteins 0.000 description 1
• 101001014220 Monascus pilosus Dehydrogenase mokE Proteins 0.000 description 1
• 108010085220 Multiprotein Complexes Proteins 0.000 description 1
• 102000007474 Multiprotein Complexes Human genes 0.000 description 1
• 101100059658 Mus musculus Cetn4 gene Proteins 0.000 description 1
• 241000234295 Musa Species 0.000 description 1
• 235000003805 Musa ABB Group Nutrition 0.000 description 1
• 235000018290 Musa x paradisiaca Nutrition 0.000 description 1
• 101000997933 Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) (2E,6E)-farnesyl diphosphate synthase Proteins 0.000 description 1
• 101001015102 Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) Dimethylallyltranstransferase Proteins 0.000 description 1
• 235000006508 Nelumbo nucifera Nutrition 0.000 description 1
• 240000002853 Nelumbo nucifera Species 0.000 description 1
• 235000006510 Nelumbo pentapetala Nutrition 0.000 description 1
• 235000002725 Olea europaea Nutrition 0.000 description 1
• 101000894711 Origanum vulgare Bicyclo-germacrene synthase Proteins 0.000 description 1
• 229940122060 Ornithine decarboxylase inhibitor Drugs 0.000 description 1
• 102000052812 Ornithine decarboxylases Human genes 0.000 description 1
• 108700005126 Ornithine decarboxylases Proteins 0.000 description 1
• 229910019142 PO4 Inorganic materials 0.000 description 1
• 235000001591 Pachyrhizus erosus Nutrition 0.000 description 1
• 244000215747 Pachyrhizus erosus Species 0.000 description 1
• 235000018669 Pachyrhizus tuberosus Nutrition 0.000 description 1
• 108010085387 Patchoulol synthase Proteins 0.000 description 1
• 241000208181 Pelargonium Species 0.000 description 1
• 101000573542 Penicillium citrinum Compactin nonaketide synthase, enoyl reductase component Proteins 0.000 description 1
• 244000038248 Pennisetum spicatum Species 0.000 description 1
• 244000115721 Pennisetum typhoides Species 0.000 description 1
• 239000006002 Pepper Substances 0.000 description 1
• 108091005804 Peptidases Proteins 0.000 description 1
• 102000035195 Peptidases Human genes 0.000 description 1
• 239000001888 Peptone Substances 0.000 description 1
• 108010080698 Peptones Proteins 0.000 description 1
• 241000218196 Persea Species 0.000 description 1
• 244000025272 Persea americana Species 0.000 description 1
• 235000008673 Persea americana Nutrition 0.000 description 1
• 244000062780 Petroselinum sativum Species 0.000 description 1
• 241000222385 Phanerochaete Species 0.000 description 1
• 241000219833 Phaseolus Species 0.000 description 1
• 235000006089 Phaseolus angularis Nutrition 0.000 description 1
• 244000100170 Phaseolus lunatus Species 0.000 description 1
• LTQCLFMNABRKSH-UHFFFAOYSA-N Phleomycin Natural products N=1C(C=2SC=C(N=2)C(N)=O)CSC=1CCNC(=O)C(C(O)C)NC(=O)C(C)C(O)C(C)NC(=O)C(C(OC1C(C(O)C(O)C(CO)O1)OC1C(C(OC(N)=O)C(O)C(CO)O1)O)C=1NC=NC=1)NC(=O)C1=NC(C(CC(N)=O)NCC(N)C(N)=O)=NC(N)=C1C LTQCLFMNABRKSH-UHFFFAOYSA-N 0.000 description 1
• 108010035235 Phleomycins Proteins 0.000 description 1
• 108010064785 Phospholipases Proteins 0.000 description 1
• 102000015439 Phospholipases Human genes 0.000 description 1
• 102100035362 Phosphomannomutase 2 Human genes 0.000 description 1
• OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
• 101100124346 Photorhabdus laumondii subsp. laumondii (strain DSM 15139 / CIP 105565 / TT01) hisCD gene Proteins 0.000 description 1
• 108010081996 Photosystem I Protein Complex Proteins 0.000 description 1
• 108010060806 Photosystem II Protein Complex Proteins 0.000 description 1
• 240000000020 Picea glauca Species 0.000 description 1
• 235000008127 Picea glauca Nutrition 0.000 description 1
• 241000218595 Picea sitchensis Species 0.000 description 1
• 235000005205 Pinus Nutrition 0.000 description 1
• 241000218602 Pinus <genus> Species 0.000 description 1
• 235000008593 Pinus contorta Nutrition 0.000 description 1
• 235000011334 Pinus elliottii Nutrition 0.000 description 1
• 241000142776 Pinus elliottii Species 0.000 description 1
• 244000019397 Pinus jeffreyi Species 0.000 description 1
• 241000555277 Pinus ponderosa Species 0.000 description 1
• 235000013269 Pinus ponderosa var ponderosa Nutrition 0.000 description 1
• 235000013268 Pinus ponderosa var scopulorum Nutrition 0.000 description 1
• 235000016761 Piper aduncum Nutrition 0.000 description 1
• 240000003889 Piper guineense Species 0.000 description 1
• 235000017804 Piper guineense Nutrition 0.000 description 1
• 235000008184 Piper nigrum Nutrition 0.000 description 1
• 241000219843 Pisum Species 0.000 description 1
• 108010064851 Plant Proteins Proteins 0.000 description 1
• 235000015266 Plantago major Nutrition 0.000 description 1
• 241000209504 Poaceae Species 0.000 description 1
• 239000002202 Polyethylene glycol Substances 0.000 description 1
• 241000985694 Polypodiopsida Species 0.000 description 1
• 235000001855 Portulaca oleracea Nutrition 0.000 description 1
• PXRCIOIWVGAZEP-UHFFFAOYSA-N Primaeres Camphenhydrat Natural products C1CC2C(O)(C)C(C)(C)C1C2 PXRCIOIWVGAZEP-UHFFFAOYSA-N 0.000 description 1
• 235000000497 Primula Nutrition 0.000 description 1
• 241000245063 Primula Species 0.000 description 1
• 108010059712 Pronase Proteins 0.000 description 1
• 239000004365 Protease Substances 0.000 description 1
• 108010076504 Protein Sorting Signals Proteins 0.000 description 1
• 108020001991 Protoporphyrinogen Oxidase Proteins 0.000 description 1
• 102000005135 Protoporphyrinogen oxidase Human genes 0.000 description 1
• 244000018633 Prunus armeniaca Species 0.000 description 1
• 235000009827 Prunus armeniaca Nutrition 0.000 description 1
• 241001290151 Prunus avium subsp. avium Species 0.000 description 1
• 235000006029 Prunus persica var nucipersica Nutrition 0.000 description 1
• 235000006040 Prunus persica var persica Nutrition 0.000 description 1
• 244000017714 Prunus persica var. nucipersica Species 0.000 description 1
• 108091008109 Pseudogenes Proteins 0.000 description 1
• 102000057361 Pseudogenes Human genes 0.000 description 1
• 235000008572 Pseudotsuga menziesii Nutrition 0.000 description 1
• 235000005386 Pseudotsuga menziesii var menziesii Nutrition 0.000 description 1
• 241000508269 Psidium Species 0.000 description 1
• 240000001679 Psidium guajava Species 0.000 description 1
• 235000013929 Psidium pyriferum Nutrition 0.000 description 1
• 244000294611 Punica granatum Species 0.000 description 1
• 235000014360 Punica granatum Nutrition 0.000 description 1
• 235000014443 Pyrus communis Nutrition 0.000 description 1
• 244000088415 Raphanus sativus Species 0.000 description 1
• 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 1
• 235000011449 Rosa Nutrition 0.000 description 1
• 235000004789 Rosa xanthina Nutrition 0.000 description 1
• 241000109329 Rosa xanthina Species 0.000 description 1
• 235000017848 Rubus fruticosus Nutrition 0.000 description 1
• 240000007651 Rubus glaucus Species 0.000 description 1
• 235000011034 Rubus glaucus Nutrition 0.000 description 1
• 235000009122 Rubus idaeus Nutrition 0.000 description 1
• 108010018903 S-linalool synthase Proteins 0.000 description 1
• 108091006629 SLC13A2 Proteins 0.000 description 1
• 235000008691 Sabina virginiana Nutrition 0.000 description 1
• 241000235070 Saccharomyces Species 0.000 description 1
• 241000209051 Saccharum Species 0.000 description 1
• 240000000111 Saccharum officinarum Species 0.000 description 1
• 235000007201 Saccharum officinarum Nutrition 0.000 description 1
• 241000195663 Scenedesmus Species 0.000 description 1
• 241000235347 Schizosaccharomyces pombe Species 0.000 description 1
• 101100097319 Schizosaccharomyces pombe (strain 972 / ATCC 24843) ala1 gene Proteins 0.000 description 1
• 241001138418 Sequoia sempervirens Species 0.000 description 1
• 235000008515 Setaria glauca Nutrition 0.000 description 1
• 235000007226 Setaria italica Nutrition 0.000 description 1
• 101150081875 Slc7a10 gene Proteins 0.000 description 1
• 235000002597 Solanum melongena Nutrition 0.000 description 1
• 244000061458 Solanum melongena Species 0.000 description 1
• 235000007230 Sorghum bicolor Nutrition 0.000 description 1
• 235000009337 Spinacia oleracea Nutrition 0.000 description 1
• 244000300264 Spinacia oleracea Species 0.000 description 1
• 235000009184 Spondias indica Nutrition 0.000 description 1
• 229920002472 Starch Polymers 0.000 description 1
• 108091081024 Start codon Proteins 0.000 description 1
• 241001112810 Streptocarpus Species 0.000 description 1
• 229930006000 Sucrose Natural products 0.000 description 1
• CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
• 235000021536 Sugar beet Nutrition 0.000 description 1
• NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
• 108030000407 Syn-copalyl-diphosphate synthases Proteins 0.000 description 1
• 108030004977 Syn-pimara-7,15-diene synthases Proteins 0.000 description 1
• 241000192707 Synechococcus Species 0.000 description 1
• 241000192584 Synechocystis Species 0.000 description 1
• 235000012308 Tagetes Nutrition 0.000 description 1
• 241000736851 Tagetes Species 0.000 description 1
• FRJSECSOXKQMOD-HQRMLTQVSA-N Taxa-4(5),11(12)-diene Chemical compound C1C[C@]2(C)CCC=C(C)[C@H]2C[C@@H]2CCC(C)=C1C2(C)C FRJSECSOXKQMOD-HQRMLTQVSA-N 0.000 description 1
• 108020005038 Terminator Codon Proteins 0.000 description 1
• 108030004268 Terpinolene synthases Proteins 0.000 description 1
• 235000006468 Thea sinensis Nutrition 0.000 description 1
• 241000218638 Thuja plicata Species 0.000 description 1
• 102000006601 Thymidine Kinase Human genes 0.000 description 1
• 108020004440 Thymidine kinase Proteins 0.000 description 1
• 240000006909 Tilia x europaea Species 0.000 description 1
• 235000011941 Tilia x europaea Nutrition 0.000 description 1
• 102000007641 Trefoil Factors Human genes 0.000 description 1
• 241000499912 Trichoderma reesei Species 0.000 description 1
• 235000015724 Trifolium pratense Nutrition 0.000 description 1
• 235000001484 Trigonella foenum graecum Nutrition 0.000 description 1
• 244000250129 Trigonella foenum graecum Species 0.000 description 1
• 235000019714 Triticale Nutrition 0.000 description 1
• 235000008554 Tsuga heterophylla Nutrition 0.000 description 1
• 241000722923 Tulipa Species 0.000 description 1
• 241000722921 Tulipa gesneriana Species 0.000 description 1
• 235000015919 Ustilago maydis Nutrition 0.000 description 1
• 235000003095 Vaccinium corymbosum Nutrition 0.000 description 1
• 235000017537 Vaccinium myrtillus Nutrition 0.000 description 1
• 108030003566 Valencene synthases Proteins 0.000 description 1
• 235000007212 Verbena X moechina Moldenke Nutrition 0.000 description 1
• 240000001519 Verbena officinalis Species 0.000 description 1
• 235000001594 Verbena polystachya Kunth Nutrition 0.000 description 1
• 235000007200 Verbena x perriana Moldenke Nutrition 0.000 description 1
• 235000002270 Verbena x stuprosa Moldenke Nutrition 0.000 description 1
• 108010053355 Vetispiradiene synthase Proteins 0.000 description 1
• 241000219873 Vicia Species 0.000 description 1
• 235000002096 Vicia faba var. equina Nutrition 0.000 description 1
• 240000002895 Vicia hirsuta Species 0.000 description 1
• 235000010711 Vigna angularis Nutrition 0.000 description 1
• 240000007098 Vigna angularis Species 0.000 description 1
• 241000863480 Vinca Species 0.000 description 1
• 241000405217 Viola <butterfly> Species 0.000 description 1
• 235000009754 Vitis X bourquina Nutrition 0.000 description 1
• 235000012333 Vitis X labruscana Nutrition 0.000 description 1
• 240000006365 Vitis vinifera Species 0.000 description 1
• 235000014787 Vitis vinifera Nutrition 0.000 description 1
• 108700040099 Xylose isomerases Proteins 0.000 description 1
• 235000007244 Zea mays Nutrition 0.000 description 1
• 101000720152 Zea mays Acyclic sesquiterpene synthase Proteins 0.000 description 1
• 240000003307 Zinnia violacea Species 0.000 description 1
• FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
• 101150067314 aadA gene Proteins 0.000 description 1
• 229930014549 abietadiene Natural products 0.000 description 1
• 108010093941 acetylxylan esterase Proteins 0.000 description 1
• 150000007513 acids Chemical class 0.000 description 1
• 239000011543 agarose gel Substances 0.000 description 1
• 238000000246 agarose gel electrophoresis Methods 0.000 description 1
• 238000013019 agitation Methods 0.000 description 1
• 239000003513 alkali Substances 0.000 description 1
• OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 description 1
• 235000020224 almond Nutrition 0.000 description 1
• 108090000637 alpha-Amylases Proteins 0.000 description 1
• XCPQUQHBVVXMRQ-UHFFFAOYSA-N alpha-Fenchene Natural products C1CC2C(=C)CC1C2(C)C XCPQUQHBVVXMRQ-UHFFFAOYSA-N 0.000 description 1
• 102000005840 alpha-Galactosidase Human genes 0.000 description 1
• 108010030291 alpha-Galactosidase Proteins 0.000 description 1
• 108010084650 alpha-N-arabinofuranosidase Proteins 0.000 description 1
• 239000011795 alpha-carotene Substances 0.000 description 1
• 235000003903 alpha-carotene Nutrition 0.000 description 1
• ANVAOWXLWRTKGA-HLLMEWEMSA-N alpha-carotene Natural products C(=C\C=C\C=C(/C=C/C=C(\C=C\C=1C(C)(C)CCCC=1C)/C)\C)(\C=C\C=C(/C=C/[C@H]1C(C)=CCCC1(C)C)\C)/C ANVAOWXLWRTKGA-HLLMEWEMSA-N 0.000 description 1
• VYBREYKSZAROCT-UHFFFAOYSA-N alpha-myrcene Natural products CC(=C)CCCC(=C)C=C VYBREYKSZAROCT-UHFFFAOYSA-N 0.000 description 1
• 229940126575 aminoglycoside Drugs 0.000 description 1
• 150000003863 ammonium salts Chemical class 0.000 description 1
• HMTAHNDPLDKYJT-CBBWQLFWSA-N amorpha-4,11-diene Chemical compound C1=C(C)CC[C@H]2[C@H](C)CC[C@@H](C(C)=C)[C@H]21 HMTAHNDPLDKYJT-CBBWQLFWSA-N 0.000 description 1
• HMTAHNDPLDKYJT-UHFFFAOYSA-N amorphadiene Natural products C1=C(C)CCC2C(C)CCC(C(C)=C)C21 HMTAHNDPLDKYJT-UHFFFAOYSA-N 0.000 description 1
• 210000004102 animal cell Anatomy 0.000 description 1
• NERNKRPBSOBEHC-UHFFFAOYSA-N anti-copalol Natural products CC1(C)CCCC2(C)C(CCC(C)=CCO)C(=C)CCC21 NERNKRPBSOBEHC-UHFFFAOYSA-N 0.000 description 1
• 230000000340 anti-metabolite Effects 0.000 description 1
• 229940121375 antifungal agent Drugs 0.000 description 1
• 239000003429 antifungal agent Substances 0.000 description 1
• 229940100197 antimetabolite Drugs 0.000 description 1
• 239000002256 antimetabolite Substances 0.000 description 1
• 235000016520 artichoke thistle Nutrition 0.000 description 1
• 235000000183 arugula Nutrition 0.000 description 1
• QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
• MXWJVTOOROXGIU-UHFFFAOYSA-N atrazine Chemical compound CCNC1=NC(Cl)=NC(NC(C)C)=N1 MXWJVTOOROXGIU-UHFFFAOYSA-N 0.000 description 1
• 230000010310 bacterial transformation Effects 0.000 description 1
• 239000011324 bead Substances 0.000 description 1
• 102000006995 beta-Glucosidase Human genes 0.000 description 1
• 108010047754 beta-Glucosidase Proteins 0.000 description 1
• 239000011648 beta-carotene Substances 0.000 description 1
• 235000013734 beta-carotene Nutrition 0.000 description 1
• TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 description 1
• NDVASEGYNIMXJL-UHFFFAOYSA-N beta-sabinene Natural products C=C1CCC2(C(C)C)C1C2 NDVASEGYNIMXJL-UHFFFAOYSA-N 0.000 description 1
• 229960002747 betacarotene Drugs 0.000 description 1
• 229960000074 biopharmaceutical Drugs 0.000 description 1
• 235000021029 blackberry Nutrition 0.000 description 1
• CXNPLSGKWMLZPZ-UHFFFAOYSA-N blasticidin-S Natural products O1C(C(O)=O)C(NC(=O)CC(N)CCN(C)C(N)=N)C=CC1N1C(=O)N=C(N)C=C1 CXNPLSGKWMLZPZ-UHFFFAOYSA-N 0.000 description 1
• 229960001561 bleomycin Drugs 0.000 description 1
• OYVAGSVQBOHSSS-UAPAGMARSA-O bleomycin A2 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC=C(N=1)C=1SC=C(N=1)C(=O)NCCC[S+](C)C)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C OYVAGSVQBOHSSS-UAPAGMARSA-O 0.000 description 1
• 235000021014 blueberries Nutrition 0.000 description 1
• 210000000081 body of the sternum Anatomy 0.000 description 1
• 239000001273 butane Substances 0.000 description 1
• 239000006227 byproduct Substances 0.000 description 1
• 235000009120 camo Nutrition 0.000 description 1
• 229930006739 camphene Natural products 0.000 description 1
• ZYPYEBYNXWUCEA-UHFFFAOYSA-N camphenilone Natural products C1CC2C(=O)C(C)(C)C1C2 ZYPYEBYNXWUCEA-UHFFFAOYSA-N 0.000 description 1
• OSQPUMRCKZAIOZ-UHFFFAOYSA-N carbon dioxide;ethanol Chemical compound CCO.O=C=O OSQPUMRCKZAIOZ-UHFFFAOYSA-N 0.000 description 1
• 150000001732 carboxylic acid derivatives Chemical class 0.000 description 1
• 150000001746 carotenes Chemical class 0.000 description 1
• 235000005473 carotenes Nutrition 0.000 description 1
• 229940117948 caryophyllene Drugs 0.000 description 1
• 108010073469 casbene synthetase Proteins 0.000 description 1
• 239000005018 casein Substances 0.000 description 1
• BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
• 235000021240 caseins Nutrition 0.000 description 1
• 235000020226 cashew nut Nutrition 0.000 description 1
• 230000001925 catabolic effect Effects 0.000 description 1
• 150000001768 cations Chemical class 0.000 description 1
• SVURIXNDRWRAFU-OGMFBOKVSA-N cedrol Chemical compound C1[C@]23[C@H](C)CC[C@H]3C(C)(C)[C@@H]1[C@@](O)(C)CC2 SVURIXNDRWRAFU-OGMFBOKVSA-N 0.000 description 1
• PCROEXHGMUJCDB-UHFFFAOYSA-N cedrol Natural products CC1CCC2C(C)(C)C3CC(C)(O)CC12C3 PCROEXHGMUJCDB-UHFFFAOYSA-N 0.000 description 1
• 229940026455 cedrol Drugs 0.000 description 1
• 230000022131 cell cycle Effects 0.000 description 1
• 230000003915 cell function Effects 0.000 description 1
• 239000013592 cell lysate Substances 0.000 description 1
• 210000000170 cell membrane Anatomy 0.000 description 1
• 210000002421 cell wall Anatomy 0.000 description 1
• 230000001413 cellular effect Effects 0.000 description 1
• 239000001913 cellulose Substances 0.000 description 1
• 229920002678 cellulose Polymers 0.000 description 1
• 108010080434 cephalosporin-C deacetylase Proteins 0.000 description 1
• 235000013339 cereals Nutrition 0.000 description 1
• 235000005607 chanvre indien Nutrition 0.000 description 1
• 235000019693 cherries Nutrition 0.000 description 1
• 108010031100 chloroplast transit peptides Proteins 0.000 description 1
• RFFOTVCVTJUTAD-UHFFFAOYSA-N cineole Natural products C1CC2(C)CCC1(C(C)C)O2 RFFOTVCVTJUTAD-UHFFFAOYSA-N 0.000 description 1
• 229960005233 cineole Drugs 0.000 description 1
• 239000013599 cloning vector Substances 0.000 description 1
• 238000000975 co-precipitation Methods 0.000 description 1
• 238000002485 combustion reaction Methods 0.000 description 1
• 238000004891 communication Methods 0.000 description 1
• 239000012141 concentrate Substances 0.000 description 1
• 238000010411 cooking Methods 0.000 description 1
• 244000038559 crop plants Species 0.000 description 1
• 210000004748 cultured cell Anatomy 0.000 description 1
• 229930186364 cyclamen Natural products 0.000 description 1
• 230000001461 cytolytic effect Effects 0.000 description 1
• 210000000805 cytoplasm Anatomy 0.000 description 1
• 230000006837 decompression Effects 0.000 description 1
• 238000000432 density-gradient centrifugation Methods 0.000 description 1
• 230000008021 deposition Effects 0.000 description 1
• 238000013461 design Methods 0.000 description 1
• 238000011161 development Methods 0.000 description 1
• 230000018109 developmental process Effects 0.000 description 1
• 230000001079 digestive effect Effects 0.000 description 1
• 235000004879 dioscorea Nutrition 0.000 description 1
• 238000007598 dipping method Methods 0.000 description 1
• 229930004069 diterpene Natural products 0.000 description 1
• 150000004141 diterpene derivatives Chemical class 0.000 description 1
• 229940079593 drug Drugs 0.000 description 1
• 239000003814 drug Substances 0.000 description 1
• VLCYCQAOQCDTCN-UHFFFAOYSA-N eflornithine Chemical compound NCCCC(N)(C(F)F)C(O)=O VLCYCQAOQCDTCN-UHFFFAOYSA-N 0.000 description 1
• 239000000806 elastomer Substances 0.000 description 1
• 229920001971 elastomer Polymers 0.000 description 1
• 238000001962 electrophoresis Methods 0.000 description 1
• 210000002472 endoplasmic reticulum Anatomy 0.000 description 1
• CECREIRZLPLYDM-UHFFFAOYSA-N ent-epimanool Natural products CC1(C)CCCC2(C)C(CCC(O)(C)C=C)C(=C)CCC21 CECREIRZLPLYDM-UHFFFAOYSA-N 0.000 description 1
• ONVABDHFQKWOSV-HPUSYDDDSA-N ent-kaur-16-ene Chemical compound C1C[C@H](C2)C(=C)C[C@@]32CC[C@@H]2C(C)(C)CCC[C@@]2(C)[C@@H]31 ONVABDHFQKWOSV-HPUSYDDDSA-N 0.000 description 1
• 230000002255 enzymatic effect Effects 0.000 description 1
• 238000012869 ethanol precipitation Methods 0.000 description 1
• 108010038658 exo-1,4-beta-D-xylosidase Proteins 0.000 description 1
• 108010093305 exopolygalacturonase Proteins 0.000 description 1
• 238000000605 extraction Methods 0.000 description 1
• 238000000855 fermentation Methods 0.000 description 1
• 230000004151 fermentation Effects 0.000 description 1
• 239000000835 fiber Substances 0.000 description 1
• 239000012530 fluid Substances 0.000 description 1
• 239000004459 forage Substances 0.000 description 1
• 238000013467 fragmentation Methods 0.000 description 1
• 238000006062 fragmentation reaction Methods 0.000 description 1
• 239000003205 fragrance Substances 0.000 description 1
• 239000000295 fuel oil Substances 0.000 description 1
• 125000000524 functional group Chemical group 0.000 description 1
• 230000004927 fusion Effects 0.000 description 1
• 235000004611 garlic Nutrition 0.000 description 1
• 238000012239 gene modification Methods 0.000 description 1
• 230000005017 genetic modification Effects 0.000 description 1
• 235000013617 genetically modified food Nutrition 0.000 description 1
• 239000011521 glass Substances 0.000 description 1
• 239000003365 glass fiber Substances 0.000 description 1
• IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 description 1
• 235000021331 green beans Nutrition 0.000 description 1
• 239000005090 green fluorescent protein Substances 0.000 description 1
• 238000010438 heat treatment Methods 0.000 description 1
• 108010002430 hemicellulase Proteins 0.000 description 1
• 230000002573 hemicellulolytic effect Effects 0.000 description 1
• 239000011487 hemp Substances 0.000 description 1
• 235000008216 herbs Nutrition 0.000 description 1
• 101150113423 hisD gene Proteins 0.000 description 1
• 125000004435 hydrogen atom Chemical group [H]* 0.000 description 1
• 229930190166 impatien Natural products 0.000 description 1
• 230000001976 improved effect Effects 0.000 description 1
• 238000010348 incorporation Methods 0.000 description 1
• PZOUSPYUWWUPPK-UHFFFAOYSA-N indole Natural products CC1=CC=CC2=C1C=CN2 PZOUSPYUWWUPPK-UHFFFAOYSA-N 0.000 description 1
• RKJUIXBNRJVNHR-UHFFFAOYSA-N indolenine Natural products C1=CC=C2CC=NC2=C1 RKJUIXBNRJVNHR-UHFFFAOYSA-N 0.000 description 1
• 229910017053 inorganic salt Inorganic materials 0.000 description 1
• 239000002917 insecticide Substances 0.000 description 1
• 229940047122 interleukins Drugs 0.000 description 1
• 239000000543 intermediate Substances 0.000 description 1
• 108010090785 inulinase Proteins 0.000 description 1
• SVURIXNDRWRAFU-UHFFFAOYSA-N juniperanol Natural products C1C23C(C)CCC3C(C)(C)C1C(O)(C)CC2 SVURIXNDRWRAFU-UHFFFAOYSA-N 0.000 description 1
• 235000021374 legumes Nutrition 0.000 description 1
• 108010091662 levopimaradiene synthase Proteins 0.000 description 1
• 239000004571 lime Substances 0.000 description 1
• 125000000396 limonene group Chemical group 0.000 description 1
• 235000019421 lipase Nutrition 0.000 description 1
• 239000003915 liquefied petroleum gas Substances 0.000 description 1
• 229910052744 lithium Inorganic materials 0.000 description 1
• 235000014684 lodgepole pine Nutrition 0.000 description 1
• 239000000314 lubricant Substances 0.000 description 1
• 235000012661 lycopene Nutrition 0.000 description 1
• 239000001751 lycopene Substances 0.000 description 1
• OAIJSZIZWZSQBC-GYZMGTAESA-N lycopene Chemical compound CC(C)=CCC\C(C)=C\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C=C(/C)CCC=C(C)C OAIJSZIZWZSQBC-GYZMGTAESA-N 0.000 description 1
• 229960004999 lycopene Drugs 0.000 description 1
• 229910001629 magnesium chloride Inorganic materials 0.000 description 1
• 238000012423 maintenance Methods 0.000 description 1
• CECREIRZLPLYDM-QGZVKYPTSA-N manool Chemical compound CC1(C)CCC[C@]2(C)[C@@H](CC[C@](O)(C)C=C)C(=C)CC[C@H]21 CECREIRZLPLYDM-QGZVKYPTSA-N 0.000 description 1
• JKMAMXHNJFUAFT-UHFFFAOYSA-N manool Natural products CC1(C)CCCC2(C)C(CCC(O)C=C)C(=C)CCC12 JKMAMXHNJFUAFT-UHFFFAOYSA-N 0.000 description 1
• 238000005259 measurement Methods 0.000 description 1
• 235000013372 meat Nutrition 0.000 description 1
• 230000021121 meiosis Effects 0.000 description 1
• 239000012528 membrane Substances 0.000 description 1
• 239000002923 metal particle Substances 0.000 description 1
• 239000006151 minimal media Substances 0.000 description 1
• 210000003470 mitochondria Anatomy 0.000 description 1
• 230000002438 mitochondrial effect Effects 0.000 description 1
• 230000011278 mitosis Effects 0.000 description 1
• 230000000394 mitotic effect Effects 0.000 description 1
• 238000001823 molecular biology technique Methods 0.000 description 1
• 238000010369 molecular cloning Methods 0.000 description 1
• 229910000403 monosodium phosphate Inorganic materials 0.000 description 1
• 235000019799 monosodium phosphate Nutrition 0.000 description 1
• 229930003658 monoterpene Natural products 0.000 description 1
• 150000002773 monoterpene derivatives Chemical class 0.000 description 1
• 235000002577 monoterpenes Nutrition 0.000 description 1
• 238000002703 mutagenesis Methods 0.000 description 1
• 231100000350 mutagenesis Toxicity 0.000 description 1
• IJDNQMDRQITEOD-UHFFFAOYSA-N n-butane Chemical compound CCCC IJDNQMDRQITEOD-UHFFFAOYSA-N 0.000 description 1
• OFBQJSOFQDEBGM-UHFFFAOYSA-N n-pentane Natural products CCCCC OFBQJSOFQDEBGM-UHFFFAOYSA-N 0.000 description 1
• 150000002823 nitrates Chemical class 0.000 description 1
• 210000004940 nucleus Anatomy 0.000 description 1
• 235000014571 nuts Nutrition 0.000 description 1
• 239000002818 ornithine decarboxylase inhibitor Substances 0.000 description 1
• 239000001301 oxygen Substances 0.000 description 1
• 229910052760 oxygen Inorganic materials 0.000 description 1
• 235000002252 panizo Nutrition 0.000 description 1
• 238000003068 pathway analysis Methods 0.000 description 1
• 230000002351 pectolytic effect Effects 0.000 description 1
• 235000019319 peptone Nutrition 0.000 description 1
• 235000011197 perejil Nutrition 0.000 description 1
• 239000000575 pesticide Substances 0.000 description 1
• 235000021317 phosphate Nutrition 0.000 description 1
• 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
• 108010082527 phosphinothricin N-acetyltransferase Proteins 0.000 description 1
• 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
• 229910052698 phosphorus Inorganic materials 0.000 description 1
• 239000011574 phosphorus Substances 0.000 description 1
• 235000021118 plant-derived protein Nutrition 0.000 description 1
• 239000013600 plasmid vector Substances 0.000 description 1
• 229920001223 polyethylene glycol Polymers 0.000 description 1
• 238000003752 polymerase chain reaction Methods 0.000 description 1
• 238000001556 precipitation Methods 0.000 description 1
• CBIDRCWHNCKSTO-UHFFFAOYSA-N prenyl diphosphate Chemical compound CC(C)=CCO[P@](O)(=O)OP(O)(O)=O CBIDRCWHNCKSTO-UHFFFAOYSA-N 0.000 description 1
• 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
• 230000001902 propagating effect Effects 0.000 description 1
• 239000001294 propane Substances 0.000 description 1
• 238000000751 protein extraction Methods 0.000 description 1
• 101150058614 psbH gene Proteins 0.000 description 1
• 235000015136 pumpkin Nutrition 0.000 description 1
• 235000003499 redwood Nutrition 0.000 description 1
• 230000001172 regenerating effect Effects 0.000 description 1
• 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
• 230000008439 repair process Effects 0.000 description 1
• 239000011347 resin Substances 0.000 description 1
• 229920005989 resin Polymers 0.000 description 1
• 230000000717 retained effect Effects 0.000 description 1
• 235000001520 savin Nutrition 0.000 description 1
• 238000005204 segregation Methods 0.000 description 1
• 239000006152 selective media Substances 0.000 description 1
• 238000012163 sequencing technique Methods 0.000 description 1
• 229930004725 sesquiterpene Natural products 0.000 description 1
• 150000004354 sesquiterpene derivatives Chemical class 0.000 description 1
• 238000010008 shearing Methods 0.000 description 1
• 230000035939 shock Effects 0.000 description 1
• 235000000673 shore pine Nutrition 0.000 description 1
• 239000001509 sodium citrate Substances 0.000 description 1
• NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
• AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
• 239000002689 soil Substances 0.000 description 1
• 244000000000 soil microbiome Species 0.000 description 1
• 238000000638 solvent extraction Methods 0.000 description 1
• 239000000600 sorbitol Substances 0.000 description 1
• 229940063673 spermidine Drugs 0.000 description 1
• 239000007921 spray Substances 0.000 description 1
• 235000020354 squash Nutrition 0.000 description 1
• 230000000087 stabilizing effect Effects 0.000 description 1
• 239000008107 starch Substances 0.000 description 1
• 235000019698 starch Nutrition 0.000 description 1
• 230000003068 static effect Effects 0.000 description 1
• 150000003431 steroids Chemical class 0.000 description 1
• 239000012536 storage buffer Substances 0.000 description 1
• 238000006467 substitution reaction Methods 0.000 description 1
• 239000005720 sucrose Substances 0.000 description 1
• 229940124530 sulfonamide Drugs 0.000 description 1
• 150000003456 sulfonamides Chemical class 0.000 description 1
• 229910052717 sulfur Inorganic materials 0.000 description 1
• 239000011593 sulfur Substances 0.000 description 1
• 239000000725 suspension Substances 0.000 description 1
• 238000003786 synthesis reaction Methods 0.000 description 1
• 230000002194 synthesizing effect Effects 0.000 description 1
• 108010087432 terpene synthase Proteins 0.000 description 1
• 150000003535 tetraterpenes Chemical class 0.000 description 1
• 235000009657 tetraterpenes Nutrition 0.000 description 1
• 231100000331 toxic Toxicity 0.000 description 1
• 230000002588 toxic effect Effects 0.000 description 1
• ZCIHMQAPACOQHT-ZGMPDRQDSA-N trans-isorenieratene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/c1c(C)ccc(C)c1C)C=CC=C(/C)C=Cc2c(C)ccc(C)c2C ZCIHMQAPACOQHT-ZGMPDRQDSA-N 0.000 description 1
• 238000012546 transfer Methods 0.000 description 1
• 230000009261 transgenic effect Effects 0.000 description 1
• 230000007704 transition Effects 0.000 description 1
• 230000032258 transport Effects 0.000 description 1
• 235000001019 trigonella foenum-graecum Nutrition 0.000 description 1
• 150000003648 triterpenes Chemical class 0.000 description 1
• 101150081616 trpB gene Proteins 0.000 description 1
• 101150111232 trpB-1 gene Proteins 0.000 description 1
• 238000002255 vaccination Methods 0.000 description 1
• 235000015112 vegetable and seed oil Nutrition 0.000 description 1
• 230000035899 viability Effects 0.000 description 1
• 108700026220 vif Genes Proteins 0.000 description 1
• 239000013603 viral vector Substances 0.000 description 1
• 229940088594 vitamin Drugs 0.000 description 1
• 239000011782 vitamin Substances 0.000 description 1
• 235000013343 vitamin Nutrition 0.000 description 1
• 229930003231 vitamin Natural products 0.000 description 1
• 241000228158 x Triticosecale Species 0.000 description 1
• 150000003735 xanthophylls Chemical class 0.000 description 1
• 235000008210 xanthophylls Nutrition 0.000 description 1
• KKOXKGNSUHTUBV-UHFFFAOYSA-N zingiberene Chemical compound CC(C)=CCCC(C)C1CC=C(C)C=C1 KKOXKGNSUHTUBV-UHFFFAOYSA-N 0.000 description 1
• OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 description 1
• DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1