JP5666908B2 - ゲノムdnaの大きな断片の捕捉および修飾、ならびに合成葉緑体を有する生物を構築するための系 - Google Patents
ゲノムdnaの大きな断片の捕捉および修飾、ならびに合成葉緑体を有する生物を構築するための系 Download PDFInfo
- Publication number
- JP5666908B2 JP5666908B2 JP2010528006A JP2010528006A JP5666908B2 JP 5666908 B2 JP5666908 B2 JP 5666908B2 JP 2010528006 A JP2010528006 A JP 2010528006A JP 2010528006 A JP2010528006 A JP 2010528006A JP 5666908 B2 JP5666908 B2 JP 5666908B2
- Authority
- JP
- Japan
- Prior art keywords
- yeast
- dna
- vectors
- vector
- chloroplast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8214—Plastid transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Cell Biology (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US97802407P | 2007-10-05 | 2007-10-05 | |
| US60/978,024 | 2007-10-05 | ||
| PCT/US2008/011540 WO2009045550A2 (en) | 2007-10-05 | 2008-10-06 | System for capturing and modifying large pieces of genomic dna and constructing organisms with synthetic chloroplasts |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JP2010539979A JP2010539979A (ja) | 2010-12-24 |
| JP2010539979A5 JP2010539979A5 (https=) | 2011-11-24 |
| JP5666908B2 true JP5666908B2 (ja) | 2015-02-12 |
Family
ID=40042337
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2010528006A Expired - Fee Related JP5666908B2 (ja) | 2007-10-05 | 2008-10-06 | ゲノムdnaの大きな断片の捕捉および修飾、ならびに合成葉緑体を有する生物を構築するための系 |
Country Status (12)
| Country | Link |
|---|---|
| EP (1) | EP2195426B1 (https=) |
| JP (1) | JP5666908B2 (https=) |
| KR (1) | KR101389254B1 (https=) |
| CN (1) | CN101939423A (https=) |
| AU (1) | AU2008307471B2 (https=) |
| CA (1) | CA2698627A1 (https=) |
| DK (1) | DK2195426T3 (https=) |
| GB (2) | GB2460351B (https=) |
| IL (1) | IL204250A (https=) |
| MX (1) | MX2010003157A (https=) |
| NZ (1) | NZ583682A (https=) |
| WO (1) | WO2009045550A2 (https=) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP5618413B2 (ja) | 2007-10-08 | 2014-11-05 | シンセティック ゲノミクス、インク. | 大型核酸のアッセンブリー |
| SG179157A1 (en) * | 2009-09-15 | 2012-05-30 | Sapphire Energy Inc | A system for transformation of the chloroplast genome of scenedesmus sp. and dunaliella sp. |
| CN104277974B (zh) * | 2013-07-03 | 2017-09-15 | 中国科学院植物研究所 | 一种分离产氢衣藻叶绿体的方法 |
| CN104419701B (zh) * | 2013-08-29 | 2019-01-11 | 天津大学 | 多片段dna的酵母快速组装方法 |
| JP6226464B2 (ja) * | 2013-09-30 | 2017-11-08 | 花王株式会社 | チオエステラーゼ改変体を用いた脂質の製造方法 |
| ES2778727T3 (es) * | 2014-08-25 | 2020-08-11 | Geneweave Biosciences Inc | Partículas de transducción no replicativas y sistemas indicadores basados en partículas de transducción |
| US11008602B2 (en) | 2017-12-20 | 2021-05-18 | Roche Molecular Systems, Inc. | Non-replicative transduction particles and transduction particle-based reporter systems |
| CN109536525B (zh) * | 2019-02-20 | 2019-08-23 | 中国科学院烟台海岸带研究所 | 一种杜氏盐藻叶绿体同源重组空载体及其应用 |
| CN110669789B (zh) * | 2019-12-04 | 2020-04-14 | 中国科学院烟台海岸带研究所 | 一种雨生红球藻叶绿体表达系统及其应用 |
| CN111440733A (zh) * | 2020-02-07 | 2020-07-24 | 天津大学 | 产松油醇的重组酿酒酵母及构建方法及应用 |
| CN115807021A (zh) * | 2022-08-01 | 2023-03-17 | 深圳大学 | 一种莱茵衣藻人工叶绿体基因组合成组装与功能测试的方法 |
| CN115948450B (zh) * | 2022-08-01 | 2024-02-13 | 深圳大学 | 一种莱茵衣藻叶绿体-酿酒酵母-大肠杆菌穿梭载体及其构建方法与应用 |
| CN116218750B (zh) * | 2022-10-14 | 2024-11-26 | 天津科技大学 | 一种解淀粉芽孢杆菌底盘菌及其构建方法与应用 |
| CN118256412B (zh) * | 2024-04-01 | 2024-09-27 | 湖北大学 | 运动发酵单胞菌的工程菌株、制备方法及应用 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7129391B1 (en) * | 1988-09-26 | 2006-10-31 | Auburn University | Universal chloroplast integration and expression vectors, transformed plants and products thereof |
| US5866404A (en) * | 1995-12-06 | 1999-02-02 | Yale University | Yeast-bacteria shuttle vector |
| US5976846A (en) * | 1996-01-13 | 1999-11-02 | Passmore; Steven E. | Method for multifragment in vivo cloning and mutation mapping |
| NZ502742A (en) * | 1997-08-07 | 2004-04-30 | Univ Auburn | Universal chloroplast integration and expression vectors, transformed plants and products thereof |
| AU5217599A (en) * | 1998-07-27 | 2000-02-21 | Genotypes, Inc. | Automatic eukaryotic artificial chromosome vector |
| AU5597200A (en) * | 1999-06-07 | 2000-12-28 | Cell Genesys, Inc. | Hybrid yeast-bacteria cloning system and uses thereof |
| CN1997743B (zh) * | 2004-03-03 | 2011-12-21 | 国立大学法人奈良先端科学技术大学院大学 | 通过叶绿体技术提高植物生产能力的方法 |
| BRPI0511046A (pt) * | 2004-05-10 | 2007-11-27 | Basf Plant Science Gmbh | métodos para produzir uma construção de expressão múltipla, para produzir alimento ou produtos alimentìcios, produtos farmacêuticos ou produtos quìmicos, e uma planta ou célula de planta transgênica, construção de expressão múltipla, microorganismo,e, uso de um microorganismo, de uma célula de planta, de um tecido de planta, de um orgão de planta, ou de uma parte de uma planta |
| EP2171085A4 (en) * | 2007-06-01 | 2010-07-14 | Sapphire Energy | SCREENING OF GENETICALLY MODIFIED PHOTOSYNTHETIC ORGANISMS WITH HIGH THROUGHPUT |
| US20090209015A1 (en) * | 2008-02-15 | 2009-08-20 | Ramesha Chakkodabylu S | Compositions and methods for production of biofuels |
-
2008
- 2008-10-06 KR KR1020107008929A patent/KR101389254B1/ko not_active Expired - Fee Related
- 2008-10-06 NZ NZ583682A patent/NZ583682A/en not_active IP Right Cessation
- 2008-10-06 CA CA2698627A patent/CA2698627A1/en not_active Abandoned
- 2008-10-06 DK DK08836521.8T patent/DK2195426T3/da active
- 2008-10-06 AU AU2008307471A patent/AU2008307471B2/en not_active Ceased
- 2008-10-06 CN CN2008801097736A patent/CN101939423A/zh active Pending
- 2008-10-06 WO PCT/US2008/011540 patent/WO2009045550A2/en not_active Ceased
- 2008-10-06 JP JP2010528006A patent/JP5666908B2/ja not_active Expired - Fee Related
- 2008-10-06 MX MX2010003157A patent/MX2010003157A/es active IP Right Grant
- 2008-10-06 GB GB0913302A patent/GB2460351B/en not_active Expired - Fee Related
- 2008-10-06 EP EP08836521.8A patent/EP2195426B1/en active Active
- 2008-10-06 GB GB0818260A patent/GB2453648C2/en not_active Expired - Fee Related
-
2010
- 2010-03-02 IL IL204250A patent/IL204250A/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| KR101389254B1 (ko) | 2014-04-24 |
| DK2195426T3 (da) | 2013-09-08 |
| EP2195426A4 (en) | 2010-12-01 |
| WO2009045550A3 (en) | 2009-07-30 |
| AU2008307471A1 (en) | 2009-04-09 |
| KR20100085930A (ko) | 2010-07-29 |
| GB2453648C (en) | 2012-12-05 |
| IL204250A (en) | 2012-12-31 |
| GB2453648A8 (en) | 2010-11-17 |
| GB0913302D0 (en) | 2009-09-02 |
| MX2010003157A (es) | 2010-05-21 |
| GB2460351B (en) | 2011-03-30 |
| WO2009045550A2 (en) | 2009-04-09 |
| GB0818260D0 (en) | 2008-11-12 |
| EP2195426B1 (en) | 2013-06-12 |
| GB2453648C2 (en) | 2012-12-19 |
| JP2010539979A (ja) | 2010-12-24 |
| NZ583682A (en) | 2012-07-27 |
| EP2195426A2 (en) | 2010-06-16 |
| GB2460351A (en) | 2009-12-02 |
| GB2453648B (en) | 2011-01-05 |
| CN101939423A (zh) | 2011-01-05 |
| AU2008307471B2 (en) | 2013-05-02 |
| GB2453648A (en) | 2009-04-15 |
| CA2698627A1 (en) | 2009-04-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP5666908B2 (ja) | ゲノムdnaの大きな断片の捕捉および修飾、ならびに合成葉緑体を有する生物を構築するための系 | |
| AU2013312352B2 (en) | Engineered transgene integration platform (ETIP) for gene targeting and trait stacking | |
| TWI424061B (zh) | 鋅指核酸酶媒介之同源重組方法 | |
| AU2009302889B2 (en) | System for capturing and modifying large pieces of genomic DNA and constructing organisms with synthetic chloroplasts | |
| US12590313B2 (en) | Methodologies and compositions for creating targeted recombination and breaking linkage between traits | |
| EA031429B1 (ru) | Сконструированные посадочные площадки для направленного воздействия на ген в растениях | |
| MX2007014611A (es) | Regulacion post-transcripcional de la expresion del gen. | |
| US20090269816A1 (en) | System for capturing and modifying large pieces of genomic DNA and constructing organisms with synthetic chloroplasts | |
| Tang et al. | Improvement of chloroplast transformation using CRISPR/Cas9 | |
| US20100050301A1 (en) | System for capturing and modifying large pieces of genomic dna and constructing vascular plants with synthetic chloroplast genomes | |
| AU2013203045B2 (en) | System for capturing and modifying large pieces of genomic DNA and constructing organisms with synthetic chloroplasts | |
| Pathi | Establishment of maize resistance to fungal diseases by host-induced gene silencing and site-directed mutagenesis | |
| Bateman | The maintenance and expression of foreign genes in the chloroplast of Chlamydomonas | |
| Robson | The construction of an expression vector for the transformation of the grape chloroplast genome | |
| EA041676B1 (ru) | Создание маиса, устойчивого к северной пятнистости листьев |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20111005 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20111005 |
|
| A131 | Notification of reasons for refusal |
Free format text: JAPANESE INTERMEDIATE CODE: A131 Effective date: 20131001 |
|
| A601 | Written request for extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A601 Effective date: 20131226 |
|
| A602 | Written permission of extension of time |
Free format text: JAPANESE INTERMEDIATE CODE: A602 Effective date: 20140109 |
|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20140401 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20141111 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20141211 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 5666908 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |
|
| R250 | Receipt of annual fees |
Free format text: JAPANESE INTERMEDIATE CODE: R250 |
|
| LAPS | Cancellation because of no payment of annual fees |