DK175203B1 - Fremgangsmåde til fremstilling af en transformeret Kluyveromyces-værtcelle, et tilsigtet poylpeptid heri samt en DNA-konstruktion og et plasmid til anvendelsei denne forbindelse - Google Patents

Fremgangsmåde til fremstilling af en transformeret Kluyveromyces-værtcelle, et tilsigtet poylpeptid heri samt en DNA-konstruktion og et plasmid til anvendelsei denne forbindelse Download PDF

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Publication number
DK175203B1
DK175203B1 DK198804221A DK422188A DK175203B1 DK 175203 B1 DK175203 B1 DK 175203B1 DK 198804221 A DK198804221 A DK 198804221A DK 422188 A DK422188 A DK 422188A DK 175203 B1 DK175203 B1 DK 175203B1
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sequence
dna
gene
host cell
kluyveromyces
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DK198804221A
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Danish (da)
English (en)
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DK422188D0 (da
DK422188A (da
Inventor
Krijn Rietveld
Johannes A Van Den Berg
Albert J J Van Ooyen
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Dsm Ip Assets Bv
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Priority claimed from US07/078,539 external-priority patent/US4943529A/en
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Publication of DK422188D0 publication Critical patent/DK422188D0/da
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01108—Lactase (3.2.1.108)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
DK198804221A 1987-07-28 1988-07-28 Fremgangsmåde til fremstilling af en transformeret Kluyveromyces-værtcelle, et tilsigtet poylpeptid heri samt en DNA-konstruktion og et plasmid til anvendelsei denne forbindelse DK175203B1 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7853987 1987-07-28
US07/078,539 US4943529A (en) 1982-05-19 1987-07-28 Kluyveromyces as a host strain

Publications (3)

Publication Number Publication Date
DK422188D0 DK422188D0 (da) 1988-07-28
DK422188A DK422188A (da) 1989-01-29
DK175203B1 true DK175203B1 (da) 2004-07-05

Family

ID=22144683

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198804221A DK175203B1 (da) 1987-07-28 1988-07-28 Fremgangsmåde til fremstilling af en transformeret Kluyveromyces-værtcelle, et tilsigtet poylpeptid heri samt en DNA-konstruktion og et plasmid til anvendelsei denne forbindelse

Country Status (20)

Country Link
EP (1) EP0301670B2 (fi)
JP (1) JPH02476A (fi)
KR (1) KR970007024B1 (fi)
CN (1) CN1026994C (fi)
AR (1) AR247761A1 (fi)
AT (1) ATE97446T1 (fi)
AU (1) AU628018B2 (fi)
CA (1) CA1323850C (fi)
DE (1) DE3885668T3 (fi)
DK (1) DK175203B1 (fi)
ES (1) ES2061632T5 (fi)
FI (1) FI107939B (fi)
GR (1) GR3034358T3 (fi)
HU (1) HU208553B (fi)
IE (1) IE62458B1 (fi)
IL (1) IL87258A (fi)
NO (1) NO302485B1 (fi)
NZ (1) NZ225597A (fi)
PT (1) PT88133B (fi)
ZA (1) ZA885536B (fi)

Families Citing this family (42)

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Publication number Priority date Publication date Assignee Title
US5010182A (en) * 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
FR2649991B2 (fr) * 1988-08-05 1994-03-04 Rhone Poulenc Sante Utilisation de derives stables du plasmide pkd1 pour l'expression et la secretion de proteines heterologues dans les levures du genre kluyveromyces
CA2017176A1 (en) * 1989-05-22 1990-11-22 Yoshitomi Pharmaceutical Industries Ltd. Albumin gene-containing plasmid, transformant carrying same, production of such transformant and production of albumin
FR2650598B1 (fr) * 1989-08-03 1994-06-03 Rhone Poulenc Sante Derives de l'albumine a fonction therapeutique
US5326700A (en) * 1990-11-06 1994-07-05 Eli Lilly And Company DNA sequences encoding t-PA derivatives with cleavable sites
FR2677996B1 (fr) * 1991-06-21 1993-08-27 Rhone Poulenc Rorer Sa Vecteurs de clonage et/ou d'expression preparation et utilisation.
FR2678636A1 (fr) * 1991-07-02 1993-01-08 Rhone Poulenc Rorer Sa Procede de production de proteines recombinantes et cellules hote utilisees.
IT1251435B (it) * 1991-09-04 1995-05-09 Sclavo Spa Vettore genetico per la integrazione multipla stabile di sequenze di dna nel genoma dei lieviti kluyveromyces lactis e saccharomyces cerevisiae e plasmidi che lo contengono.
FR2686899B1 (fr) 1992-01-31 1995-09-01 Rhone Poulenc Rorer Sa Nouveaux polypeptides biologiquement actifs, leur preparation et compositions pharmaceutiques les contenant.
FR2692907B1 (fr) * 1992-06-25 1995-06-30 Rhone Poulenc Rorer Sa Levures kluyveromyces modifiees, preparation et utilisation.
FR2693475B1 (fr) * 1992-07-08 1994-09-02 Rhone Poulenc Rorer Sa Procédé d'identification et/ou de clonage de promoteurs transcriptionnels, et utilisation de ces promoteurs pour l'expression de gènes.
GB9526733D0 (en) 1995-12-30 1996-02-28 Delta Biotechnology Ltd Fusion proteins
GB9907461D0 (en) 1999-03-31 1999-05-26 King S College London Neurite regeneration
US6265186B1 (en) 1997-04-11 2001-07-24 Dsm N.V. Yeast cells comprising at least two copies of a desired gene integrated into the chromosomal genome at more than one non-ribosomal RNA encoding domain, particularly with Kluyveromyces
CN1059236C (zh) * 1997-04-24 2000-12-06 复旦大学 脆壁克鲁维酵母的高效转化系统
US20050079573A1 (en) 1998-12-23 2005-04-14 Danisco A/S Proteins
US6946134B1 (en) 2000-04-12 2005-09-20 Human Genome Sciences, Inc. Albumin fusion proteins
AU2001259063A1 (en) 2000-04-12 2001-10-30 Human Genome Sciences, Inc. Albumin fusion proteins
GB0020498D0 (en) 2000-08-18 2000-10-11 Sterix Ltd Compound
CN100558898C (zh) 2000-09-21 2009-11-11 巴斯福股份公司 篮霉菌木聚糖酶
CA2706364A1 (en) 2000-12-07 2002-06-13 Dsm Ip Assets B.V. Method for the prevention or reduction of haze in beverages
DE60132801T2 (de) 2000-12-07 2009-02-12 Dsm Ip Assets B.V. Prolylendoprotease aus aspergillus niger
GB0116453D0 (en) 2001-07-05 2001-08-29 Imp College Innovations Ltd Method
DK1578771T3 (da) 2001-10-10 2013-06-10 Novo Nordisk As Remodellering og glycokonjugering af peptider
CN105131104B (zh) 2001-10-10 2018-11-16 诺和诺德公司 肽的重构和糖缀合
EP1463751B1 (en) 2001-12-21 2013-05-22 Human Genome Sciences, Inc. Albumin fusion proteins
CA2490020C (en) 2002-06-07 2011-01-04 Dsm Ip Assets B.V. Improved method for the prevention or reduction of haze in beverages
TWI328612B (en) 2002-07-25 2010-08-11 Dsm Ip Assets Bv Genes and their encoded polypeptides involved in the biosynthetic pathway of vitamin b12, vectors and host cells comprising the genes, and process for producing vitamin b12
EP2338333B1 (en) 2003-04-09 2017-09-06 ratiopharm GmbH Glycopegylation methods and proteins/peptides produced by the methods
GB0403864D0 (en) 2004-02-20 2004-03-24 Ucl Ventures Modulator
ES2246175B1 (es) * 2004-07-30 2007-03-16 Felix Claverie Martin "procedimiento para producir quimosina caprina recombinante para la coagulacion de la leche de cabra en la fabricacion de queso".
EP2439266B1 (en) 2005-11-28 2017-02-01 DSM IP Assets B.V. Enzyme preparations yielding a clean taste
GB0600406D0 (en) 2006-01-10 2006-02-15 Univ Bath Crystal
EP1816201A1 (en) 2006-02-06 2007-08-08 CSL Behring GmbH Modified coagulation factor VIIa with extended half-life
MX2008013182A (es) 2006-04-13 2008-10-27 Dsm Ip Assets Bv Composicion liquida que comprende una proteasa aspartica.
CA2676686C (en) 2007-02-20 2015-12-15 Dsm Ip Assets B.V. Novel sialidase
DK2126060T3 (en) 2007-03-22 2017-01-16 Dsm Ip Assets Bv Hitherto unknown lysyl oxidases
US8198089B2 (en) 2008-03-18 2012-06-12 Yamaguchi University Flocculent yeast and method for production thereof
WO2011101339A2 (en) 2010-02-18 2011-08-25 Dsm Ip Assets B.V. Fermentation broth and filtration filter cake and uses thereof
WO2012007445A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Mutarotase in crystallization
WO2012007446A1 (en) 2010-07-13 2012-01-19 Dsm Ip Assets B.V. Use of a mutarotase in the production of dried powders
CN115806889B (zh) * 2022-09-28 2024-06-11 山东大学 一种提高基因表达水平的酿酒酵母工程菌及其构建方法与应用

Family Cites Families (5)

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Publication number Priority date Publication date Assignee Title
IL66614A (en) * 1981-08-28 1985-09-29 Genentech Inc Method of constructing a dna sequence encoding a polypeptide,microbial production of human serum albumin,and pharmaceutical compositions comprising it
SU1662352A3 (ru) * 1982-05-05 1991-07-07 Генентек, Инк (Фирма) Штамм бактерий ЕSснеRIснIа coLI - продуцент активатора плазминогена тканевого типа
IE55817B1 (en) * 1982-05-19 1991-01-30 Gist Brocades Nv Cloning system for kluyveromyces species
EP0116201B1 (en) * 1983-01-12 1992-04-22 Chiron Corporation Secretory expression in eukaryotes
IT1203758B (it) * 1986-03-27 1989-02-23 Univ Roma Vettori di clonazione e di espressione di geni eterologhi in lieviti e lieviti trasformati con tali vettori

Also Published As

Publication number Publication date
ATE97446T1 (de) 1993-12-15
AU2014888A (en) 1989-03-02
AR247761A1 (es) 1995-03-31
EP0301670B1 (en) 1993-11-18
KR970007024B1 (ko) 1997-05-02
IL87258A (en) 1994-02-27
PT88133A (pt) 1989-06-30
ES2061632T5 (es) 2000-12-16
NZ225597A (en) 1991-08-27
KR890002407A (ko) 1989-04-10
FI107939B (fi) 2001-10-31
GR3034358T3 (en) 2000-12-29
HUT47637A (en) 1989-03-28
PT88133B (pt) 1995-03-01
DK422188D0 (da) 1988-07-28
HU208553B (en) 1993-11-29
IE882314L (en) 1989-01-28
DE3885668T3 (de) 2000-08-17
CN1026994C (zh) 1994-12-14
EP0301670B2 (en) 2000-06-14
NO883347D0 (no) 1988-07-28
ZA885536B (en) 1989-04-26
AU628018B2 (en) 1992-09-10
DE3885668D1 (de) 1993-12-23
CA1323850C (en) 1993-11-02
FI883540A (fi) 1989-01-29
FI883540A0 (fi) 1988-07-28
CN1040052A (zh) 1990-02-28
ES2061632T3 (es) 1994-12-16
IL87258A0 (en) 1988-12-30
DE3885668T2 (de) 1994-05-05
EP0301670A1 (en) 1989-02-01
IE62458B1 (en) 1995-02-08
NO302485B1 (no) 1998-03-09
DK422188A (da) 1989-01-29
JPH02476A (ja) 1990-01-05
NO883347L (no) 1989-01-30

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