DK169680B1 - Fremgangsmåde til adskillelse af vævsplasminogenaktivatorarter - Google Patents

Fremgangsmåde til adskillelse af vævsplasminogenaktivatorarter Download PDF

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Publication number
DK169680B1
DK169680B1 DK418487A DK418487A DK169680B1 DK 169680 B1 DK169680 B1 DK 169680B1 DK 418487 A DK418487 A DK 418487A DK 418487 A DK418487 A DK 418487A DK 169680 B1 DK169680 B1 DK 169680B1
Authority
DK
Denmark
Prior art keywords
tpa
fraction
daltons
eluent
approx
Prior art date
Application number
DK418487A
Other languages
Danish (da)
English (en)
Other versions
DK418487A (da
DK418487D0 (da
Inventor
Mitsuyoshi Morii
Masaharu Ohoka
Toshihiko Suzuki
Katsuyuki Suzuki
Nobuhiro Kawashima
Noriko Morii
Kunizou Mori
Original Assignee
Mitsui Toatsu Chemicals
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mitsui Toatsu Chemicals filed Critical Mitsui Toatsu Chemicals
Publication of DK418487D0 publication Critical patent/DK418487D0/da
Publication of DK418487A publication Critical patent/DK418487A/da
Application granted granted Critical
Publication of DK169680B1 publication Critical patent/DK169680B1/da

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/6456—Plasminogen activators
    • C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21069—Protein C activated (3.4.21.69)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/814—Enzyme separation or purification
    • Y10S435/815—Enzyme separation or purification by sorption

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
DK418487A 1986-08-11 1987-08-11 Fremgangsmåde til adskillelse af vævsplasminogenaktivatorarter DK169680B1 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP18685186 1986-08-11
JP61186851A JP2507339B2 (ja) 1986-08-11 1986-08-11 粗ｔＰＡの精製方法

Publications (3)

Publication Number Publication Date
DK418487D0 DK418487D0 (da) 1987-08-11
DK418487A DK418487A (da) 1988-02-12
DK169680B1 true DK169680B1 (da) 1995-01-09

Family

ID=16195757

Family Applications (1)

Application Number Title Priority Date Filing Date
DK418487A DK169680B1 (da) 1986-08-11 1987-08-11 Fremgangsmåde til adskillelse af vævsplasminogenaktivatorarter

Country Status (10)

Country Link
US (1) US5141863A (ja)
EP (1) EP0256836B1 (ja)
JP (1) JP2507339B2 (ja)
AU (1) AU586756B2 (ja)
CA (1) CA1302928C (ja)
DE (1) DE3780883T2 (ja)
DK (1) DK169680B1 (ja)
FI (1) FI88308C (ja)
NO (1) NO175011C (ja)
ZA (1) ZA875902B (ja)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01238534A (ja) * 1988-03-17 1989-09-22 Mitsui Toatsu Chem Inc エンドトキシンの除去方法
US7544500B2 (en) 1999-11-13 2009-06-09 Talecris Biotherapeutics, Inc. Process for the production of a reversibly inactive acidified plasmin composition
US6355243B1 (en) 1999-11-13 2002-03-12 Bayer Corporation Method of thrombolysis by local delivery of active plasmin
US20020064860A1 (en) * 2000-11-29 2002-05-30 Schering Corporation Method for purifying adenoviruses
EP2287174B8 (en) * 2008-01-18 2016-12-07 Bio-Rad Laboratories, Inc. Enhanced purification of antibody fragments by apatite chromatography
KR20110017903A (ko) 2008-06-04 2011-02-22 테일크리스 바이오쎄러퓨틱스 아이엔씨. 플라스민의 제조를 위한 조성물, 방법 및 키트
PL2403865T3 (pl) 2009-03-03 2016-01-29 Grifols Therapeutics Inc Sposoby wytwarzania plazminogenu
EP2524215B1 (en) 2010-01-15 2018-12-26 Bio-Rad Laboratories, Inc. Surface neutralization of apatite
US8895707B2 (en) 2010-08-18 2014-11-25 Bio-Rad Laboratories, Inc. Elution of proteins from hydroxyapatite resins without resin deterioration
US9592540B2 (en) 2011-02-02 2017-03-14 Bio-Rad Laboratories, Inc. Apatite surface neutralization with alkali solutions
CN102286449B (zh) * 2011-05-30 2013-04-03 云南省地方病防治所 鼠疫菌纤溶酶原激活因子Pla的提取与纯化方法
CN104507952B (zh) 2012-05-30 2018-08-10 生物辐射实验室股份有限公司 基于磷灰石的色谱树脂的原位恢复
WO2015200299A2 (en) 2014-06-23 2015-12-30 Bio-Rad Laboratories, Inc. Apatite in-situ restoration
WO2015200282A1 (en) 2014-06-23 2015-12-30 Bio-Rad Laboratories, Inc. Apatite pretreatment
CN111909920B (zh) * 2020-08-24 2022-04-15 武汉人福药业有限责任公司 一种重组人组织型纤溶酶原激活剂的表达纯化方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3542646A (en) * 1966-11-22 1970-11-24 Green Cross Corp Process for fractionally obtaining urokinase and blood coagulation accelerator in human urine
US3904480A (en) * 1972-10-24 1975-09-09 Lilly Co Eli Enhanced production of plasminogen activator
IL43343A (en) * 1972-10-24 1976-03-31 Lilly Co Eli Production of plasminogen activator
JPS55130918A (en) * 1979-03-30 1980-10-11 Toubishi Yakuhin Kogyo Kk Plasminogen-activating agent and its preparation
JPS57146580A (en) * 1981-03-09 1982-09-10 Seikagaku Kogyo Co Ltd Separation and purification of enzyme in human urine
DE3382389D1 (de) * 1982-12-14 1991-10-02 South African Inventions Plasminogenaktivator.
US4960702A (en) * 1985-09-06 1990-10-02 Codon Methods for recovery of tissue plasminogen activator
US4898825A (en) * 1986-05-07 1990-02-06 Mitsui Toatsu Chemicals, Incorporated Methods for purification of single-chain and double-chain tissue plasminogen activator
JPS6379591A (ja) * 1986-09-22 1988-04-09 Mitsui Toatsu Chem Inc ｔＰＡの精製方法
US4928620A (en) * 1988-09-01 1990-05-29 Currey Lesley B Seat pedestal mount

Also Published As

Publication number Publication date
ZA875902B (en) 1988-02-12
NO175011B (no) 1994-05-09
NO873332D0 (no) 1987-08-10
DK418487A (da) 1988-02-12
NO175011C (no) 1994-08-17
FI873470A (fi) 1988-02-12
NO873332L (no) 1988-02-12
US5141863A (en) 1992-08-25
CA1302928C (en) 1992-06-09
DE3780883D1 (de) 1992-09-10
FI88308B (fi) 1993-01-15
EP0256836A1 (en) 1988-02-24
DK418487D0 (da) 1987-08-11
EP0256836B1 (en) 1992-08-05
JPS6342687A (ja) 1988-02-23
AU586756B2 (en) 1989-07-20
JP2507339B2 (ja) 1996-06-12
FI88308C (fi) 1993-04-26
DE3780883T2 (de) 1993-02-04
AU7666687A (en) 1988-02-18
FI873470A0 (fi) 1987-08-10

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