DK1490480T3 - Primitive og proksimale leverstamceller - Google Patents
Primitive og proksimale leverstamceller Download PDFInfo
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- DK1490480T3 DK1490480T3 DK03728245.6T DK03728245T DK1490480T3 DK 1490480 T3 DK1490480 T3 DK 1490480T3 DK 03728245 T DK03728245 T DK 03728245T DK 1490480 T3 DK1490480 T3 DK 1490480T3
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- cells
- liver
- cell
- stem cell
- liver stem
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Claims (16)
1. Sammensætning, som omfatter en isoleret human primitiv leverstamcelle, hvor den isolerede primitive leverstamcelle ikke udtrykker alfa-føtoprotein, men udtrykker Ep-CAM, og hvor den isolerede primitive leverstamcelle er en precursor for en proksimal leverstamcelle, der er i stand til at være ophav til en hepatocytær progenitorcelle eller en biliær progenitorcelle, og idet den isolerede primitive leverstamcelle er en voksen enkeltcelle.
2. Sammensætning ifølge krav 1, hvor den isolerede humane primitive leverstamcelle udtrykker AC133 og albumin.
3. Sammensætning ifølge krav 2, hvor den isolerede humane primitive leverstamcelle udtrykker cytokeratin 8/18, cytokeratin 19, eller kombinationer deraf.
4. Sammensætning ifølge krav 3, hvor den isolerede humane primitive leverstamcelle udtrykker N-CAM, CAM5.2, c-kit, CD 146, eller kombinationer deraf.
5. Fremgangsmåde til isolering afen human primitiv leverprogenitorcelle, som kan være ophav til såvel hepatocytære progenitorceller som biliære progenitorceller, hvilken fremgangsmåde omfatter: (a) at tilvejebringe en suspension af celler, som stammer fra levervæv; (b) at overføre cellesuspensionen til en plastoverflade; (c) at dyrke cellerne under betingelser, der indbefatter anvendelse af et serumfrit medium, der omfatter en regulator af carbohydrat-metabolisme, en jernkilde og en membranproducerende faktor; og (d) at selektere de celler, der udtrykker Ep-CAM, og ikke udtrykker alfa-føtoprotein.
6. Fremgangsmåde ifølge krav 5, som yderligere omfatter selektion fra suspensionen af de celler, der udtrykker albumin, cytokeratin 19, AC133, cytokeratin 8/18, eller kombinationer deraf.
7. Fremgangsmåde ifølge krav 5, hvor levervævet er opnået fra et foster eller et nyfødt individ.
8. Fremgangsmåde ifølge krav 5, hvor levervævet er opnået fra et barn eller en voksen.
9. Fremgangsmåde ifølge krav 5 eller 6, som yderligere omfatter selektion fra suspensionen af de celler, der udtrykker N-CAM, CAM5.2, c-kit, CD146, eller kombinationer deraf.
10. Sammensætning ifølge et hvilket som helst af kravene 1 til 4, hvor den humane primitive leverstamcelle omfatter en eksogen nukleinsyre.
11. Isoleret human primitiv leverstamcelle, som ikke udtrykker alfa-føtoprotein, som udtrykker Ep-CAM, og som er i stand til at være ophav til såvel hepatocytære progenitorceller som biliære progenitorceller, idet den isolerede primitive leverstamcelle er en voksen enkeltcelle.
12. Isoleret human primitiv leverstamcelle ifølge krav 11, som yderligere udtrykker AC133 og albumin.
13. Isoleret human primitiv leverstamcelle ifølge krav 12, som yderligere udtrykker cytokeratin 8/18, cytokeratin 19, eller kombinationer deraf.
14. Isoleret human primitiv leverstamcelle ifølge krav 12, som yderligere udtrykker N-CAM, CAM5.2, c-kit, CD146, eller kombinationer deraf.
15. Isoleret human primitiv leverstamcelle ifølge et hvilket som helst af kravene 12 til 14, som yderligere omfatter en eksogen nukleinsyre.
16. Fremgangsmåde til fremstilling af et lægemiddel til behandling af leverdysfunktion eller -sygdom, hvilken fremgangsmåde omfatter blanding af en effektiv mængde af den humane primitive leverstamcelle ifølge et hvilket som helst af kravene 11 til 15 med en farmaceutisk acceptabel bærer, en farmaceutisk acceptabel excipiens, eller et farmaceutisk acceptabelt fortyndingsmiddel.
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| IL164048A0 (en) * | 2002-03-15 | 2005-12-18 | Univ North Carolina | Primitive and proximal hepatic stemcells |
| WO2005012512A1 (en) | 2003-08-01 | 2005-02-10 | Norimasa Nakamura | Scaffold-free self-organized 3d synthetic tissue |
| WO2007083504A1 (ja) * | 2003-08-01 | 2007-07-26 | Norimasa Nakamura | スキャフォールドフリー自己組織性三次元人工組織（Ｓｃａｆｆｏｌｄ－ｆｒｅｅ Ｓｅｌｆ－Ｏｒｇａｎｉｚｅｄ ３Ｄ ｓｙｎｔｈｅｔｉｃ ｔｉｓｓｕｅ） |
| US7622108B2 (en) * | 2004-04-23 | 2009-11-24 | Bioe, Inc. | Multi-lineage progenitor cells |
| ATE506431T1 (de) * | 2004-04-23 | 2011-05-15 | Bioe Llc | Mehrfachlinien-vorläuferzellen |
| WO2006126219A1 (en) * | 2005-05-26 | 2006-11-30 | Fresenius Medical Care Deutschland G.M.B.H. | Liver progenitor cells |
| CA2630355A1 (en) * | 2005-11-16 | 2007-05-24 | University Of North Carolina At Chapel Hill | Extracellular matrix components for expansion or differentiation of hepatic progenitors |
| RU2008130098A (ru) * | 2005-12-22 | 2010-01-27 | Веста Терапьютикс, Инк. (Us) | Способ лечения нарушения функции печени с использованием печеночных клеток-предшественников |
| US7727763B2 (en) * | 2006-04-17 | 2010-06-01 | Bioe, Llc | Differentiation of multi-lineage progenitor cells to respiratory epithelial cells |
| WO2007127698A2 (en) * | 2006-04-25 | 2007-11-08 | Board Of Supervisors Of Louisiana State Universityand Agricultural And Mechanical College | Adipose derived adult stem cells in hepatic regeneration |
| PT2029725T (pt) | 2006-05-26 | 2018-04-12 | Univ North Carolina Chapel Hill | Precursores de células hepáticas estreladas e métodos para isolar as mesmas |
| IN2010KN00157A (da) * | 2007-06-15 | 2015-08-28 | Univ North Carolina | |
| EP2192908A4 (en) * | 2007-07-25 | 2010-09-01 | Bioe Inc | DIFFERENTIATION OF PRECURSOR CELLS OF MULTIPLE ABSTRACTS AGAINST CHONDROCYTES |
| US20100087000A1 (en) * | 2008-05-02 | 2010-04-08 | Vesta Therapeutics, Inc. | Hepatic progenitor cell and method of isolating same |
| JP5822287B2 (ja) * | 2008-05-14 | 2015-11-24 | 公立大学法人横浜市立大学 | ヒト肝幹細胞、その調製方法、分化誘導方法及び利用方法 |
| CA2724839A1 (en) * | 2008-05-21 | 2009-11-26 | Bioe Llc | Differentiation of multi-lineage progenitor cells to pancreatic cells |
| MX2010012730A (es) | 2008-05-22 | 2011-03-01 | Vesta Therapeutics Inc | Metodo para diferenciacion de celulas progenitoras de mamifero en celulas de islote pancreaticas que producen insulina. |
| US20100227393A1 (en) * | 2009-03-06 | 2010-09-09 | Eric Lagasse | Liver stem cells: isolation of hepatic progenitor cells from the human gall bladder |
| BR112012009848B1 (pt) * | 2009-10-30 | 2021-11-03 | The University Of North Carolina At Chapel Hill | Método de obtenção de células-tronco/progenitoras multipotentes de mamíferos capazes de se diferenciar em linhagens hepáticas e pancreáticas |
| US9341550B2 (en) * | 2010-11-19 | 2016-05-17 | On-Chip Biotechnologies Co., Ltd. | Method for detecting low concentrations of specific cell from high concentrations of cell populations, and method for collecting and analyzing detected cell |
| AU2012262382B2 (en) * | 2011-05-27 | 2016-10-13 | DePuy Synthes Products, Inc. | Bioartificial proximal tubule systems and methods of use |
| CN104105493A (zh) | 2011-12-08 | 2014-10-15 | 耶达研究及发展有限公司 | 哺乳动物胎儿肺细胞以及其治疗用途 |
| WO2013192342A1 (en) * | 2012-06-20 | 2013-12-27 | Celula, Inc. | Materials and methods for processing cell populations |
| JP6510162B2 (ja) * | 2013-01-21 | 2019-05-08 | テルモ株式会社 | シート状細胞培養物の製造方法 |
| US9533013B2 (en) * | 2013-03-13 | 2017-01-03 | University Of North Carolina At Chapel Hill | Method of treating pancreatic and liver conditions by endoscopic-mediated (or laparoscopic-mediated) transplantation of stem cells into/onto bile duct walls of particular regions of the biliary tree |
| EP3685884A1 (en) * | 2013-09-19 | 2020-07-29 | The Board of Trustees of the Leland Stanford Junior University | Methods and compositions for producing hepatocyte-like cells |
| JP6643993B2 (ja) | 2013-12-16 | 2020-02-12 | フレゼニウス メディカル ケア ドイッチェランド ゲゼルシャフト ミット ベシュレンクテル ハフツング | 膵島様細胞構造体及びそれを調製する方法 |
| JP6595157B2 (ja) * | 2014-03-04 | 2019-10-23 | 富士フイルム株式会社 | 細胞撮像制御装置および方法並びにプログラム |
| US10179896B2 (en) | 2015-05-12 | 2019-01-15 | Baker Group, LLP | Method and system for a bioartificial organ |
| WO2016200340A1 (en) * | 2015-06-12 | 2016-12-15 | Agency For Science, Technology And Research | Derivation of hepatic stem cells and mature liver cell types and uses thereof |
| US10668109B2 (en) | 2015-06-18 | 2020-06-02 | Yeda Research And Development Co. Ltd. | Conditioning protocols and use of same for tissue regeneration |
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| US10767164B2 (en) | 2017-03-30 | 2020-09-08 | The Research Foundation For The State University Of New York | Microenvironments for self-assembly of islet organoids from stem cells differentiation |
| EP3743080A4 (en) * | 2018-01-26 | 2021-10-27 | Polarityte, Inc. | SELF-ASSEMBLY MATERIALS COORDINATED WITH A COMPLEX LIVING INTERFACE (CLICSAM) |
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| US5665589A (en) * | 1988-12-14 | 1997-09-09 | The United States Of America As Represented By The Department Of Health And Human Services | Human liver epithelial cell lines |
| EP0597964A4 (en) * | 1991-08-07 | 1994-11-30 | Einstein Coll Med | PROLIFERATION OF HEPATOCYTE PRECURSORS. |
| EP1688037A3 (en) * | 1993-11-19 | 2006-12-13 | Albert Einstein College Of Medicine Of Yeshiva University | Hepatoblasts and method of isolating same |
| ZA951199B (en) * | 1994-02-14 | 1996-08-14 | Kirin Brewery | Protein having tpo activity |
| US5843633A (en) * | 1996-04-26 | 1998-12-01 | Amcell Corporation | Characterization of a human hematopoietic progenitor cell antigen |
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| IL164048A0 (en) * | 2002-03-15 | 2005-12-18 | Univ North Carolina | Primitive and proximal hepatic stemcells |
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