DK1141371T3 - Fremgangsmåder til fremstilling af polypeptider i Aspergillus-mutantceller - Google Patents

Fremgangsmåder til fremstilling af polypeptider i Aspergillus-mutantceller

Info

Publication number
DK1141371T3
DK1141371T3 DK99960956T DK99960956T DK1141371T3 DK 1141371 T3 DK1141371 T3 DK 1141371T3 DK 99960956 T DK99960956 T DK 99960956T DK 99960956 T DK99960956 T DK 99960956T DK 1141371 T3 DK1141371 T3 DK 1141371T3
Authority
DK
Denmark
Prior art keywords
mutant
methods
polypeptide
mutant cells
producing polypeptides
Prior art date
Application number
DK99960956T
Other languages
Danish (da)
English (en)
Inventor
Svend Kaasgaard
Henrik Moellgaard
Bjorn Eggert Christensen
Jan Lehmbeck
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK1141371T3 publication Critical patent/DK1141371T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/145—Fungal isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/66—Aspergillus
    • C12R2001/69—Aspergillus oryzae
DK99960956T 1998-12-23 1999-12-22 Fremgangsmåder til fremstilling af polypeptider i Aspergillus-mutantceller DK1141371T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA199801726 1998-12-23
DKPA199900745 1999-05-27
PCT/DK1999/000726 WO2000039322A1 (fr) 1998-12-23 1999-12-22 Procedes permettant de produire des polypeptides dans des cellules mutantes d'aspergillus

Publications (1)

Publication Number Publication Date
DK1141371T3 true DK1141371T3 (da) 2009-02-02

Family

ID=26064565

Family Applications (1)

Application Number Title Priority Date Filing Date
DK99960956T DK1141371T3 (da) 1998-12-23 1999-12-22 Fremgangsmåder til fremstilling af polypeptider i Aspergillus-mutantceller

Country Status (9)

Country Link
EP (1) EP1141371B1 (fr)
JP (2) JP2002533133A (fr)
CN (1) CN100529096C (fr)
AT (1) ATE414782T1 (fr)
AU (1) AU1774000A (fr)
DE (1) DE69939947D1 (fr)
DK (1) DK1141371T3 (fr)
ES (1) ES2317706T3 (fr)
WO (1) WO2000039322A1 (fr)

Families Citing this family (79)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003070957A2 (fr) * 2002-02-20 2003-08-28 Novozymes A/S Production de polypeptides de plantes
DK2294928T3 (da) 2002-10-11 2014-11-10 Novozymes As Fremgangsmåde til fremstilling af et varmebehandlet produkt
WO2004090549A1 (fr) * 2003-04-11 2004-10-21 Novozymes A/S Procede de criblage pour des polypeptides glycosyles secretes
NZ542924A (en) 2003-04-28 2008-01-31 Novozymes As Method of producing a phospholipase which comprises processing an expressed fungal peptide so as to cleave off a peptide from the C- terminal end and/or a peptide from the N-terminal end to obtain a core peptide
CN102994486A (zh) 2003-10-23 2013-03-27 诺维信公司 在洗涤剂中具有改善稳定性的蛋白酶
EP1814996A2 (fr) 2004-11-19 2007-08-08 Novozymes A/S Polypeptides antimicrobiens et polynucleotides les codant
EP2365068B1 (fr) 2004-12-22 2017-03-01 Novozymes A/S Enzymes pour le traitement de l'amidon
CA2595056A1 (fr) 2005-01-24 2006-07-27 Dsm Ip Assets B.V. Procede de fabrication d'un compose d'interet dans une cellule fongique filamenteuse
EP1739428A1 (fr) * 2005-07-01 2007-01-03 Institut Pasteur Méthode de diagnostic in vitro et kit pour une aspergillose
ATE514770T1 (de) 2005-10-17 2011-07-15 Novozymes As Verwendung von pilzmutanten zur expression von antikörpern
EP2035561A1 (fr) 2006-06-29 2009-03-18 DSMIP Assets B.V. Procédé pour obtenir une expression de polypeptides améliorée
CN103451164A (zh) 2006-07-14 2013-12-18 诺维信股份有限公司 用于产生具有生物学活性的分泌型多肽的方法
JP5410981B2 (ja) * 2007-10-19 2014-02-05 公益財団法人野田産業科学研究所 ポリケタイドシンターゼ・ノンリボゾーマルペプチドシンテターゼ遺伝子
ES2526867T3 (es) 2008-11-20 2015-01-16 Novozymes Inc. Polipéptido que tiene actividad potenciadora amilolítica y polinucleótidos que codifican el mismo
EP2389437B1 (fr) 2009-01-21 2015-10-21 Novozymes A/S Polypeptides dotés d'activité estérase et acides nucléiques les codant
US8658409B2 (en) 2009-03-24 2014-02-25 Novozymes A/S Polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
CA2758404A1 (fr) 2009-04-22 2010-10-28 Dsm Ip Assets B.V. Procede de production d'un polypeptide recombinant d'interet
BRPI1015536A2 (pt) 2009-04-24 2019-07-02 Dsm Ip Assets Bv polipeptídeo de degradação de carboidratos e usos do mesmo
WO2011009700A1 (fr) 2009-07-22 2011-01-27 Dsm Ip Assets B.V. Cellule hôte améliorée destinée à la production d'un composé intéressant
CN102666846A (zh) 2009-09-30 2012-09-12 诺维信公司 具有纤维素分解增强活性的多肽和编码该多肽的多核苷酸
EP2496693B1 (fr) 2009-11-06 2017-10-25 Novozymes, Inc. Polypeptides ayant une activité cellobiohydrolase et polynucléotides codant pour les polypeptides
CN102639697B (zh) 2009-11-06 2015-03-25 诺维信股份有限公司 具有木聚糖酶活性的多肽和编码该多肽的多核苷酸
DK2496694T3 (en) 2009-11-06 2017-06-06 Novozymes Inc COMPOSITIONS FOR SACCHARIFYING CELLULOS MATERIAL
EA201201125A1 (ru) 2010-02-11 2013-03-29 ДСМ АйПи АССЕТС Б.В. Клетка-хозяин, способная продуцировать ферменты, пригодные для деградации лигноцеллюлозного материала
WO2011098580A1 (fr) 2010-02-11 2011-08-18 Dsm Ip Assets B.V. Polypeptide ayant une activité cellobiohydrolase et ses utilisations
CN103068978B (zh) 2010-06-25 2017-01-18 诺维信公司 具有前导序列功能的多核苷酸
CN103068988A (zh) 2010-06-25 2013-04-24 诺维信公司 具有启动子活性的多核苷酸
US8802412B2 (en) 2010-06-25 2014-08-12 Novozymes A/S Polynucleotides having promoter activity
US9109213B2 (en) 2010-06-29 2015-08-18 Dsm Ip Assets B.V. Polypeptide having carbohydrate degrading activity and uses thereof
EA201300059A1 (ru) 2010-06-29 2013-05-30 ДСМ АйПи АССЕТС Б.В. Полипептид, обладающий активностью сволленина, и его применение
AU2011273684B2 (en) 2010-06-29 2014-08-28 Dsm Ip Assets B.V. Polypeptide having beta-glucosidase activity and uses thereof
EA201300056A1 (ru) 2010-06-29 2013-07-30 ДСМ АйПи АССЕТС Б.В. Полипептид с активностью ацетилксиланэстеразы и его применения
US9441214B2 (en) 2010-06-29 2016-09-13 Dsm Ip Assets B.V. Polypeptide having beta-glucosidase activity and uses thereof
ES2628345T3 (es) 2010-06-29 2017-08-02 Dsm Ip Assets B.V. Polipéptido que tiene o que ayuda en la actividad de degradación de materiales glucídicos y usos del mismo
DK2588604T3 (en) 2010-06-30 2016-09-26 Novozymes Inc Polypeptides having beta-glucosidase activity and polynucleotides encoding them
CA2803222A1 (fr) * 2010-07-01 2012-01-05 Dsm Ip Assets B.V. Procede de production d'un compose particulier
US9057086B2 (en) 2010-08-12 2015-06-16 Novozymes, Inc. Compositions comprising a polypeptide having cellulolytic enhancing activity and a bicycle compound and uses thereof
US9267126B2 (en) 2010-08-30 2016-02-23 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
WO2012030811A1 (fr) 2010-08-30 2012-03-08 Novozymes A/S Polypeptides ayant une activité cellobiohydrolase et polynucléotides codant pour ceux-ci
US9139823B2 (en) 2010-11-12 2015-09-22 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
US9506048B2 (en) 2011-01-26 2016-11-29 Novozymes, Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
MX337942B (es) 2011-01-26 2016-03-29 Novozymes As Polipeptidos que tienen actividad de endoglucanasa y polinucleotidos que codifican los mismos.
WO2012103293A1 (fr) 2011-01-26 2012-08-02 Novozymes A/S Polypeptides ayant une activité cellobiohydrolase et polynucléotides codant pour ceux-ci
WO2012103288A1 (fr) 2011-01-26 2012-08-02 Novozymes A/S Polypeptides possédant une activité de cellobiohydrolase et polynucléotides les codant
MX2013007720A (es) 2011-01-26 2013-08-09 Novozymes As Polipeptidos que tienen actividad celobiohidrolasa y polinucleotidos que codifican para los mismos.
CN103608461B (zh) 2011-03-09 2016-08-03 诺维信公司 增加多肽的纤维素分解增强活性的方法
US9926547B2 (en) 2011-04-28 2018-03-27 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
BR112013027463A2 (pt) 2011-04-29 2017-09-26 Novozymes Inc métodos para degradar ou converter, e para fermentar um material celulóliso, para produzir um produto de fermentação, e para limpar ou lavar uma superfície dura ou roupa suja, e, composição de detergente
EP2527448A1 (fr) 2011-05-23 2012-11-28 Novozymes A/S Intégrations simultanées spécifiques au site de copies de plusieurs gènes dans des champignons filamenteux
CN102321700A (zh) * 2011-08-03 2012-01-18 无锡赛德生物工程有限公司 一种曲酸发酵工艺及发酵设备
EP2748314B1 (fr) 2011-08-24 2016-12-28 Novozymes, Inc. Compositions d'enzymes cellulolytiques d'aspergillus fumigatus et leurs utilisations
EP2773656B1 (fr) 2011-10-31 2019-06-19 Novozymes, Inc. Polypeptides présentant une activité cellulolytique augmentée et polynucléotides codant pour ceux-ci
EP2802651B1 (fr) 2011-11-21 2017-03-08 Novozymes, Inc. Variants du polypeptide gh61 et polynucléotides codant pour ceux-ci
CN113234695A (zh) 2012-04-27 2021-08-10 诺维信股份有限公司 Gh61多肽变体以及编码其的多核苷酸
CN104884615B (zh) 2012-09-05 2019-07-12 诺维信公司 具有蛋白酶活性的多肽
BR112015005884A2 (pt) 2012-09-19 2017-08-08 Novozymes Inc E Novozymes As processos para degradação de um material celulósico, para produção de um produto da fermentação, e de fermentação de um material celulósico, composição, e, formulação de caldo inteiro ou composição de cultura de células
EP2903412B1 (fr) 2012-10-08 2019-09-11 Novozymes A/S Polypeptides ayant une activité améliorant la cellulolyse et polynucléotides codant pour ceux-ci
US20150275194A1 (en) 2012-10-24 2015-10-01 Novozymes A/S Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same
WO2014093835A1 (fr) 2012-12-14 2014-06-19 Novozymes A/S Polypeptides à activité favorisant la cellulolyse et polynucléotides codant pour ces polypeptides
CN105164254B (zh) 2013-03-08 2019-06-28 诺维信公司 纤维二糖水解酶变体和编码它们的多核苷酸
CN105283546A (zh) 2013-05-10 2016-01-27 诺维信公司 具有木聚糖酶活性的多肽以及对其进行编码的多核苷酸
US11390898B2 (en) 2014-09-05 2022-07-19 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
CN114717217A (zh) 2014-12-19 2022-07-08 诺维信公司 包括具有木聚糖酶活性的多肽和具有阿拉伯呋喃糖苷酶活性的多肽的组合物
WO2016138167A2 (fr) 2015-02-24 2016-09-01 Novozymes A/S Variants de cellobiohydrolase et polynucléotides codant pour ces derniers
CN116676293A (zh) 2015-05-27 2023-09-01 国投生物科技投资有限公司 具有纤维二糖水解酶活性的多肽以及对其进行编码的多核苷酸
CN108350044B (zh) 2015-09-22 2022-05-24 诺维信公司 具有纤维二糖水解酶活性的多肽以及对其进行编码的多核苷酸
SG11201911802TA (en) * 2017-06-07 2020-01-30 Ptt Global Chemical Public Co Ltd Mutant strain aspergillus aculeatus for producing cellulase and xylanase and preparation method thereof
WO2019038418A1 (fr) 2017-08-25 2019-02-28 Novozymes A/S Extraction d'huile de palme brute assistée par des enzymes
CN112004931A (zh) 2018-03-26 2020-11-27 诺维信公司 真菌伴侣蛋白
CN108823178B (zh) * 2018-06-08 2021-12-10 中国中医科学院中药研究所 大黄素糖基转移酶蛋白FtUGT73BE5及其编码基因与应用
CN108795903B (zh) * 2018-06-08 2020-12-15 南京林业大学 一种高酶活淀粉酶的表达方法
CN108753748B (zh) * 2018-06-08 2021-12-10 中国中医科学院中药研究所 大黄素糖基转移酶蛋白FtUGT75R2及其编码基因与应用
EP3894551A1 (fr) 2018-12-12 2021-10-20 Novozymes A/S Polypeptides dotés d'une activité xylanase et polynucléotides codant pour ces polypeptides
DK3898985T3 (da) 2018-12-21 2023-04-17 Novozymes As Proteinekspression i tandem
CN110218750B (zh) * 2019-04-24 2023-04-11 郑州轻工业学院 杂色曲霉菌株在发酵生产曲酸中应用
CN110106152B (zh) * 2019-04-30 2021-08-17 中国农业科学院油料作物研究所 杂交瘤细胞株ytt-2及其产生的抗环匹阿尼酸单克隆抗体
CN110108874B (zh) * 2019-04-30 2022-09-20 中国农业科学院油料作物研究所 快速检测环匹阿尼酸的胶体金免疫分析试纸条、制备及其应用
CN114391038A (zh) 2019-07-25 2022-04-22 诺维信公司 丝状真菌表达系统
CN111139189B (zh) * 2020-01-14 2022-04-19 浙江工业大学 曲霉wbx-38及其在生产环匹阿尼酸中的应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU695766B2 (en) * 1993-12-01 1998-08-20 Novo Nordisk Biotech, Inc. (Aspergillus) expression system
WO1995015390A1 (fr) * 1993-12-01 1995-06-08 Novo Nordisk Biotech, Inc. SYSTEME D'EXPRESSION A $i(ASPERGILLUS FOETIDUS)
JP4436934B2 (ja) * 1996-09-13 2010-03-24 ノボザイムス，インコーポレイティド 変更された量のポリペプチドを生成するｄｎａ挿入突然変異を有する細胞
JP4091119B2 (ja) * 1996-09-19 2008-05-28 ノボザイムス アクティーゼルスカブ 新規な宿主細胞およびタンパク質生産方法

Also Published As

Publication number Publication date
JP2011188863A (ja) 2011-09-29
DE69939947D1 (de) 2009-01-02
WO2000039322A1 (fr) 2000-07-06
EP1141371B1 (fr) 2008-11-19
CN1333837A (zh) 2002-01-30
ES2317706T3 (es) 2009-04-16
JP2002533133A (ja) 2002-10-08
JP5453343B2 (ja) 2014-03-26
AU1774000A (en) 2000-07-31
EP1141371A1 (fr) 2001-10-10
ATE414782T1 (de) 2008-12-15
CN100529096C (zh) 2009-08-19

Similar Documents

Publication Publication Date Title
DK1141371T3 (da) Fremgangsmåder til fremstilling af polypeptider i Aspergillus-mutantceller
DK0941349T3 (da) Fremgangsmåde til fremstilling af polypeptider i surfactinmutanter af Bacillusceller
AU4200897A (en) Novel host cells and methods of producing proteins
ES8202362A1 (es) Procedimiento para preparar un acido desoxi-ribonucleico (adn) recombinante
WO1998014602A3 (fr) Preparation d'acides amines d par des materiaux et des procedes de fermentation directe
EP0764719A3 (fr) Méthodes pour culture de cellules animales
ATE210190T1 (de) Verfahren zur herstellung von 1,3-propandiol aus kohlenhydraten unter verwendung gemischter mikrobieller kulturen
AU4940096A (en) Host cell expressing reduced levels of a metalloprotease and methods using the host cell in protein production
DK1078081T3 (da) Fremgangsmåde til fremstilling af heterologe polypeptider i trichothecen deficiente filamentöse svampe mutantceller
WO2003010304A3 (fr) Procede de production de populations cellulaires isolees, milieu de culture conçu pour la culture de populations cellulaires et populations cellulaires
AU4114596A (en) A process of producing extracellular proteins in bacteria
EP2281892A3 (fr) Procédé de préparer des polypeptides dans de cellules Trichoderma avec des déficiences respiratoires
WO2004011609A3 (fr) Methodes de production de substances biologiques dans des mutants deficients en pigment de cellules de bacillus
NZ522337A (en) Production of TcR gamma delta T cells
EP1225217A4 (fr) Procede de production de transglutaminase derivee d'un micro-organisme
AU1609900A (en) Method for the massive culture of cells producing recombinant human erythropoietin
FI964208A (fi) Menetelmä metallofosfaatin saostumisen kontrolloimiseksi fermentoinneissa, joissa solutiheys on suuri
AU2002214085A1 (en) Method for producing proteins by electroporation
DK340882A (da) Fremgangsmaade til fremstilling af sur protease og mutanter af slaegten rhizopus, som danner denne
WO2000042203A3 (fr) Procede de production de polypeptides dans des cellules deficitaires en cyclohexadepsipeptide
MX9601087A (es) Gen que participa en el mecanismo de secrecion de esterasa.
AU2099800A (en) Production of proteins
AU2001251065A1 (en) Method for screening of microorganisms under nutrient limited conditions
EP0323838A3 (fr) Vecteur d'expression et procédé de préparation réglable des protéins en eucaryotes