DK3898985T3 - Proteinekspression i tandem - Google Patents

Proteinekspression i tandem Download PDF

Info

Publication number
DK3898985T3
DK3898985T3 DK19817783.4T DK19817783T DK3898985T3 DK 3898985 T3 DK3898985 T3 DK 3898985T3 DK 19817783 T DK19817783 T DK 19817783T DK 3898985 T3 DK3898985 T3 DK 3898985T3
Authority
DK
Denmark
Prior art keywords
tandem
protein expression
protein
expression
Prior art date
Application number
DK19817783.4T
Other languages
English (en)
Other versions
DK3898985T5 (da
Inventor
Richard Jan Steven Baerends
Carsten Lillelund Olsen
Josè Arnau
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of DK3898985T3 publication Critical patent/DK3898985T3/da
Application granted granted Critical
Publication of DK3898985T5 publication Critical patent/DK3898985T5/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DK19817783.4T 2018-12-21 2019-12-17 Proteinekspression i tandem DK3898985T5 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP18215558 2018-12-21
PCT/EP2019/085553 WO2020127198A1 (en) 2018-12-21 2019-12-17 Tandem protein expression

Publications (2)

Publication Number Publication Date
DK3898985T3 true DK3898985T3 (da) 2023-04-17
DK3898985T5 DK3898985T5 (da) 2024-08-05

Family

ID=64959159

Family Applications (1)

Application Number Title Priority Date Filing Date
DK19817783.4T DK3898985T5 (da) 2018-12-21 2019-12-17 Proteinekspression i tandem

Country Status (6)

Country Link
US (1) US20220073898A1 (da)
EP (1) EP3898985B1 (da)
CN (1) CN113316641A (da)
DK (1) DK3898985T5 (da)
FI (1) FI3898985T3 (da)
WO (1) WO2020127198A1 (da)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4192946A1 (en) * 2020-08-10 2023-06-14 Inbiose N.V. Cellular production of di- and/or oligosaccharides

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK122686D0 (da) 1986-03-17 1986-03-17 Novo Industri As Fremstilling af proteiner
US5989870A (en) 1986-04-30 1999-11-23 Rohm Enzyme Finland Oy Method for cloning active promoters
JP3649338B2 (ja) 1994-06-03 2005-05-18 ノボザイムス バイオテック，インコーポレイティド 精製されたマイセリオフトラ・ラッカーゼ及びそれをコードする核酸
ATE294871T1 (de) 1994-06-30 2005-05-15 Novozymes Biotech Inc Nicht-toxisches, nicht-toxigenes, nicht- pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren
US5827719A (en) * 1994-10-26 1998-10-27 Novo Nordisk A/S Enzyme with lipolytic activity
WO2000024883A1 (en) 1998-10-26 2000-05-04 Novozymes A/S Constructing and screening a dna library of interest in filamentous fungal cells
DK1141371T3 (da) 1998-12-23 2009-02-02 Novozymes As Fremgangsmåder til fremstilling af polypeptider i Aspergillus-mutantceller
CN100482801C (zh) 1999-03-22 2009-04-29 诺沃奇梅兹有限公司 用于在真菌细胞中表达基因的启动子
BRPI0509212A (pt) * 2004-03-25 2007-08-28 Genencor Int proteìna de fusão de celulase e construto de fusão de celulase heterólogo codificando a mesma
US7527947B2 (en) * 2004-06-14 2009-05-05 Novozymes A/S Signal peptide for producing a polypeptide
CN101952303A (zh) * 2008-02-25 2011-01-19 诺维信公司 提高目标蛋白质的表达产率的方法
WO2010039889A2 (en) 2008-09-30 2010-04-08 Novozymes, Inc. Methods for using positively and negatively selectable genes in a filamentous fungal cell
EP2527448A1 (en) 2011-05-23 2012-11-28 Novozymes A/S Simultaneous site-specific integrations of multiple gene-copies in filamentous fungi
ES2631605T3 (es) * 2011-11-25 2017-09-01 Novozymes A/S Polipéptidos con actividad de lisozima y polinucleótidos que codifican los mismos
WO2013178674A1 (en) 2012-05-31 2013-12-05 Novozymes A/S Improved selection in fungi
WO2015085920A1 (en) * 2013-12-11 2015-06-18 Novozymes A/S Cutinase variants and polynucleotides encoding same
CN104962573A (zh) * 2015-07-08 2015-10-07 北京嘉万生物技术有限公司 Pgⅱ与mbp融合蛋白的可溶性分泌表达及其应用
BR112019017271A2 (pt) 2017-02-20 2020-04-14 Novozymes As enzima lipolítica para uso em panificação

Also Published As

Publication number Publication date
CN113316641A (zh) 2021-08-27
US20220073898A1 (en) 2022-03-10
EP3898985B1 (en) 2023-02-08
EP3898985A1 (en) 2021-10-27
FI3898985T3 (fi) 2023-05-05
WO2020127198A1 (en) 2020-06-25
DK3898985T5 (da) 2024-08-05

Similar Documents

Publication Publication Date Title
DK3777455T3 (da) Sidelink-styreinformation i flere trin
DK3368655T3 (da) Forbedret proteinekspression og fremgangsmåder dertil
DK3663292T3 (da) Diphenylmethan-derivat i krystallinsk form
DK3416974T3 (da) Proteinoprensning
DK3233907T3 (da) Bispecifikke heterodimeriske proteiner hos gnavere
DK3737402T5 (da) Modificeret protein
DK3259358T3 (da) Forøget proteinekspression
MA50908A (fr) Formulations de protéines de fusion vegfr-fc
DK3262168T3 (da) Værtscelleproteinmodifikation
MA52349A (fr) Proteines de levures
DK3737403T3 (da) Modificerede adenovira
DK3609348T3 (da) Tekstureret proteinprodukt
UY4713S (es) Configuración aplicada en cuchara lacre
DK3380487T3 (da) Additivsystemer til anvendelse i proteinpegylering
DK3898985T3 (da) Proteinekspression i tandem
DK3271724T3 (da) Anvendelse af en standard til påvisning af proteinaggregater i en proteinfejlfoldningssygdom
DK3630793T3 (da) Rekombinant protein
FR3036923B1 (fr) Hydrolysat de proteines de poissons
UY4712S (es) Configuración aplicada en jarra
JP1635114S (ja) 椅子
UA38301S (uk) Стілець
DOS2018000099S (es) Catamarán
DOS2018000095S (es) Catamarán
DOS2018000100S (es) Catamarán
DOS2018000098S (es) Catamarán