DK1002080T3 - Flourescerende proteiner som celletype-specifikke meldere - Google Patents

Flourescerende proteiner som celletype-specifikke meldere

Info

Publication number
DK1002080T3
DK1002080T3 DK98940092T DK98940092T DK1002080T3 DK 1002080 T3 DK1002080 T3 DK 1002080T3 DK 98940092 T DK98940092 T DK 98940092T DK 98940092 T DK98940092 T DK 98940092T DK 1002080 T3 DK1002080 T3 DK 1002080T3
Authority
DK
Denmark
Prior art keywords
cells
cell
human mammal
cell type
fluorescent proteins
Prior art date
Application number
DK98940092T
Other languages
Danish (da)
English (en)
Inventor
Juergen Hescheler
Original Assignee
Axiogenesis Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Axiogenesis Ag filed Critical Axiogenesis Ag
Application granted granted Critical
Publication of DK1002080T3 publication Critical patent/DK1002080T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/05Animals comprising random inserted nucleic acids (transgenic)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00Use of cells in diagnostics
    • C12N2503/02Drug screening
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00Vector systems having a special element relevant for transcription
    • C12N2830/008Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
DK98940092T 1997-07-02 1998-06-30 Flourescerende proteiner som celletype-specifikke meldere DK1002080T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19727962A DE19727962A1 (de) 1997-07-02 1997-07-02 Fluoreszierende Proteine als zelltypspezifische Reporter
PCT/EP1998/003988 WO1999001552A1 (de) 1997-07-02 1998-06-30 Fluoreszierende proteine als zelltypspezifische reporter

Publications (1)

Publication Number Publication Date
DK1002080T3 true DK1002080T3 (da) 2003-05-26

Family

ID=7834239

Family Applications (1)

Application Number Title Priority Date Filing Date
DK98940092T DK1002080T3 (da) 1997-07-02 1998-06-30 Flourescerende proteiner som celletype-specifikke meldere

Country Status (9)

Country Link
US (2) US7105344B2 (ja)
EP (1) EP1002080B9 (ja)
JP (1) JP3956154B2 (ja)
AT (1) ATE234357T1 (ja)
DE (2) DE19727962A1 (ja)
DK (1) DK1002080T3 (ja)
ES (1) ES2191324T3 (ja)
PT (1) PT1002080E (ja)
WO (1) WO1999001552A1 (ja)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19727962A1 (de) * 1997-07-02 1999-01-14 Juergen Hescheler Fluoreszierende Proteine als zelltypspezifische Reporter
US7410798B2 (en) 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
MXPA02004954A (es) * 1999-11-19 2003-02-27 Cold Spring Harbor Lab Proteina fluorescente de expresion en ratones transgenicos bajo el control del promotor de nestina.
US7455983B2 (en) 2000-01-11 2008-11-25 Geron Corporation Medium for growing human embryonic stem cells
GB0014009D0 (en) * 2000-06-08 2000-08-02 Devgen Nv Compound screens relating to insulin deficiency or insulin resistance
US6576464B2 (en) 2000-11-27 2003-06-10 Geron Corporation Methods for providing differentiated stem cells
DE10136702B4 (de) * 2000-12-27 2004-04-22 Axiogenesis Ag System zur zell- und entwicklungsspezifischen Selektion differenzierender embryonaler Stammzellen, adulter Stammzellen und embryonaler Keimbahnzellen
WO2002051987A1 (de) * 2000-12-27 2002-07-04 Axiogenesis Ag System zur zell-und entwicklungsspezifischen selektion differenzierender embryonaler stammzellen; adulter stammzellen und embryonaler keinbahnzellen
WO2002074925A2 (en) * 2001-03-20 2002-09-26 University Of Virginian Patent Foundation Methods for identifying and purifying smooth muscle progenitor cells
BR0312764A (pt) * 2002-07-26 2005-07-26 Wisconsin Alumni Res Found Cardiomiócitos funcionais originados de células-tronco embrionárias humanas
EP1627912A4 (en) * 2003-05-20 2007-12-12 Riken PREPARATION OF ENDODERMAL STEM CELLS
EP2578676A1 (en) * 2003-06-20 2013-04-10 Axiogenesis Ag Tissue modeling in multi- or pluripotent cell systems
JP2009513106A (ja) 2003-07-08 2009-04-02 アキシオジェネシス エージー 細胞分化のためのマーカーとしての分泌タンパク質
WO2005005621A2 (en) * 2003-07-08 2005-01-20 Axiogenesis Ag NOVEL METHOD FOR THE PREPARATION OF EMBRYOID BODIES (EBs) AND USES THEREOF
DK1740945T3 (en) 2004-04-07 2019-01-21 Ncardia Ag KKE-INVASIVE, IN-VITRO FUNCTIONAL TISSUE TEST SYSTEMS
EP1745144B1 (en) 2004-05-11 2010-12-01 Axiogenesis Ag Assay for drug discovery based on in vitro differentiated cells
US20060292694A1 (en) * 2005-06-22 2006-12-28 Roslin Institute Reporter hepatocytes and other cells for drug screening and toxicity testing
EP1893749A1 (en) * 2005-06-22 2008-03-05 Geron Corporation Reporter hepatocytes and other cells for drug screening and toxicity testing
US20060292695A1 (en) * 2005-06-22 2006-12-28 Roslin Institute Methods and kits for drug screening and toxicity testing using promoter-reporter cells derived from embryonic stem cells
JP5860805B2 (ja) * 2010-03-23 2016-02-16 オリンパス株式会社 幹細胞の分化状態をモニタリングする方法
ITPI20100085A1 (it) 2010-07-07 2012-01-08 Sara Migliarini Procedimento per l'ingegnerizzazione genetica di cellule staminali che attivano un tracciante fluorescente vitale in seguito al differenziamento in vitro delle cellule stesse verso il tipo cellulare specifico dei neuroni serotoninergici.
JP2014176364A (ja) * 2013-03-15 2014-09-25 Olympus Corp 幹細胞の解析方法

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6670449B1 (en) * 1997-02-13 2003-12-30 Memorial Sloan-Kettering Cancer Center Hybrid molecules and their use for optically detecting changes in cellular microenvironments
DE19727962A1 (de) * 1997-07-02 1999-01-14 Juergen Hescheler Fluoreszierende Proteine als zelltypspezifische Reporter
WO2002051987A1 (de) * 2000-12-27 2002-07-04 Axiogenesis Ag System zur zell-und entwicklungsspezifischen selektion differenzierender embryonaler stammzellen; adulter stammzellen und embryonaler keinbahnzellen
EP2578676A1 (en) * 2003-06-20 2013-04-10 Axiogenesis Ag Tissue modeling in multi- or pluripotent cell systems
WO2005005621A2 (en) * 2003-07-08 2005-01-20 Axiogenesis Ag NOVEL METHOD FOR THE PREPARATION OF EMBRYOID BODIES (EBs) AND USES THEREOF

Also Published As

Publication number Publication date
DE19727962A1 (de) 1999-01-14
US20020092035A1 (en) 2002-07-11
ATE234357T1 (de) 2003-03-15
WO1999001552A1 (de) 1999-01-14
JP2002508670A (ja) 2002-03-19
PT1002080E (pt) 2003-06-30
EP1002080B1 (de) 2003-03-12
EP1002080B9 (de) 2004-08-11
US20060168665A1 (en) 2006-07-27
JP3956154B2 (ja) 2007-08-08
EP1002080A1 (de) 2000-05-24
US7105344B2 (en) 2006-09-12
ES2191324T3 (es) 2003-09-01
DE59807479D1 (de) 2003-04-17

Similar Documents

Publication Publication Date Title
DK1002080T3 (da) Flourescerende proteiner som celletype-specifikke meldere
Wu et al. Dynamic pluripotent stem cell states and their applications
RU2009143635A (ru) Улучшение титра полипептида фактора viii в клеточных культурах
ES2059361T3 (es) Complejo de proteinas recombinantes que tiene actividad de factor viii:c su produccion y uso.
EA200501774A1 (ru) Культуры e1-иммортализованных клеток и способы их культивирования с целью повышения выхода их продукции
DE602008002593D1 (de) Verfahren zur herstellung eines rekombinanten polyklonalen proteins
WO2006127809A3 (en) Methods for expansion and analysis of cultured hematopoietic stem cells
ATE375385T1 (de) Verwendung eines serumfreien zellkulturmediums zur produktion von il-18bp in säugerzellen
WO2004090096A3 (en) Methods for neural differentiation of embryonic stem cells using protease passaging techniques
ATE341626T1 (de) Membranproteine aus säugetierzellen; verwandte reagentien
ATE378398T1 (de) Pluripotente embryonale keimzellinie aus vögeln
ATE222605T1 (de) Bioluminiscente lyophilisierte bakterien-reagenz zur giftstoff nachweit
DE60144021D1 (de) Für zellzyklusproteine kodierende nukleinsäure und deren verwendung
ATE546531T1 (de) Transaktivierungssystem für säugerzellen
CN105874058A (zh) 多肽组合物及使用其的多能干细胞的培养方法
DE502005004379D1 (de) Gegen den Marburg I Polymorphismus der Faktor VII aktivierenden Protease (FSAP) gerichtete Antikörper, ihre Herstellung und Verwendung
Yasugi Regulation of pepsinogen gene expression in epithelial cells of vertebrate stomach during development
ATE415487T1 (de) Biologisches testverfahren für thyroid stimulierende antikörper
DE602004023408D1 (de) Agments unter verwendung einer e.coli-signalsequenz sowie verfahren zur massenproduktion des antikörperfragments
BR0008187A (pt) Controle biológico de nematódeos
DK1644485T3 (da) Udskilte proteiner som markører for celledifferentiering
Arakaki et al. Frontier dental research on iPS cells
JP5177431B2 (ja) TRACP5bの製造方法
EA200101093A1 (ru) Экспрессия гепарин-связывающего протеина в рекомбинантных клетках млекопитающих
CN109402178A (zh) 一种小鼠精原干细胞高效重编程的方法及应用