ES2191324T3 - Proteinas fluorescentes como marcadores especificos de tipos celulares. - Google Patents
Proteinas fluorescentes como marcadores especificos de tipos celulares.Info
- Publication number
- ES2191324T3 ES2191324T3 ES98940092T ES98940092T ES2191324T3 ES 2191324 T3 ES2191324 T3 ES 2191324T3 ES 98940092 T ES98940092 T ES 98940092T ES 98940092 T ES98940092 T ES 98940092T ES 2191324 T3 ES2191324 T3 ES 2191324T3
- Authority
- ES
- Spain
- Prior art keywords
- cells
- cell
- human mammal
- fluorescent proteins
- specific markers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 102000034287 fluorescent proteins Human genes 0.000 title abstract 2
- 108091006047 fluorescent proteins Proteins 0.000 title abstract 2
- 230000001413 cellular effect Effects 0.000 title 1
- 238000000034 method Methods 0.000 abstract 4
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 238000004113 cell culture Methods 0.000 abstract 2
- 230000009261 transgenic effect Effects 0.000 abstract 2
- 108020004414 DNA Proteins 0.000 abstract 1
- 230000023715 cellular developmental process Effects 0.000 abstract 1
- 238000012258 culturing Methods 0.000 abstract 1
- 230000001419 dependent effect Effects 0.000 abstract 1
- 230000018109 developmental process Effects 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000000126 substance Substances 0.000 abstract 1
- 230000002110 toxicologic effect Effects 0.000 abstract 1
- 231100000027 toxicology Toxicity 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2503/00—Use of cells in diagnostics
- C12N2503/02—Drug screening
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
La invención se refiere a células madre embrionarias (células ES) de mamíferos no humanos, que se transfectan de manera estable con un producto de recombinación de ADN, que comprende una secuencia de ADN que codifica una proteína fluorescente que no altera las células y un promotor dependiente de la célula o del desarrollo, combinado operativamente a dicha secuencia de ADN. La invención se refiere también a un procedimiento para producir dichas células ES; a un cultivo celular que se puede obtener cultivando células ES; a un procedimiento de verificación toxicológica de sustancias utilizando dichos cultivos celulares; a un procedimiento de producción de mamíferos no humanos transgénicos utilizando células ES; a un mamífero no humano transgénico que se puede obtener según dicho procedimiento y a un procedimiento para examinar las etapas del desarrollo celular utilizando células procedentes de un mamífero no humano.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19727962A DE19727962A1 (de) | 1997-07-02 | 1997-07-02 | Fluoreszierende Proteine als zelltypspezifische Reporter |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2191324T3 true ES2191324T3 (es) | 2003-09-01 |
Family
ID=7834239
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES98940092T Expired - Lifetime ES2191324T3 (es) | 1997-07-02 | 1998-06-30 | Proteinas fluorescentes como marcadores especificos de tipos celulares. |
Country Status (9)
Country | Link |
---|---|
US (2) | US7105344B2 (es) |
EP (1) | EP1002080B9 (es) |
JP (1) | JP3956154B2 (es) |
AT (1) | ATE234357T1 (es) |
DE (2) | DE19727962A1 (es) |
DK (1) | DK1002080T3 (es) |
ES (1) | ES2191324T3 (es) |
PT (1) | PT1002080E (es) |
WO (1) | WO1999001552A1 (es) |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE19727962A1 (de) * | 1997-07-02 | 1999-01-14 | Juergen Hescheler | Fluoreszierende Proteine als zelltypspezifische Reporter |
US7410798B2 (en) | 2001-01-10 | 2008-08-12 | Geron Corporation | Culture system for rapid expansion of human embryonic stem cells |
US6667176B1 (en) | 2000-01-11 | 2003-12-23 | Geron Corporation | cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells |
MXPA02004954A (es) * | 1999-11-19 | 2003-02-27 | Cold Spring Harbor Lab | Proteina fluorescente de expresion en ratones transgenicos bajo el control del promotor de nestina. |
US7455983B2 (en) | 2000-01-11 | 2008-11-25 | Geron Corporation | Medium for growing human embryonic stem cells |
GB0014009D0 (en) * | 2000-06-08 | 2000-08-02 | Devgen Nv | Compound screens relating to insulin deficiency or insulin resistance |
US6576464B2 (en) | 2000-11-27 | 2003-06-10 | Geron Corporation | Methods for providing differentiated stem cells |
DE10136702B4 (de) * | 2000-12-27 | 2004-04-22 | Axiogenesis Ag | System zur zell- und entwicklungsspezifischen Selektion differenzierender embryonaler Stammzellen, adulter Stammzellen und embryonaler Keimbahnzellen |
WO2002051987A1 (de) * | 2000-12-27 | 2002-07-04 | Axiogenesis Ag | System zur zell-und entwicklungsspezifischen selektion differenzierender embryonaler stammzellen; adulter stammzellen und embryonaler keinbahnzellen |
WO2002074925A2 (en) * | 2001-03-20 | 2002-09-26 | University Of Virginian Patent Foundation | Methods for identifying and purifying smooth muscle progenitor cells |
BR0312764A (pt) * | 2002-07-26 | 2005-07-26 | Wisconsin Alumni Res Found | Cardiomiócitos funcionais originados de células-tronco embrionárias humanas |
EP1627912A4 (en) * | 2003-05-20 | 2007-12-12 | Riken | PREPARATION OF ENDODERMAL STEM CELLS |
EP2578676A1 (en) * | 2003-06-20 | 2013-04-10 | Axiogenesis Ag | Tissue modeling in multi- or pluripotent cell systems |
JP2009513106A (ja) | 2003-07-08 | 2009-04-02 | アキシオジェネシス エージー | 細胞分化のためのマーカーとしての分泌タンパク質 |
WO2005005621A2 (en) * | 2003-07-08 | 2005-01-20 | Axiogenesis Ag | NOVEL METHOD FOR THE PREPARATION OF EMBRYOID BODIES (EBs) AND USES THEREOF |
DK1740945T3 (en) | 2004-04-07 | 2019-01-21 | Ncardia Ag | KKE-INVASIVE, IN-VITRO FUNCTIONAL TISSUE TEST SYSTEMS |
EP1745144B1 (en) | 2004-05-11 | 2010-12-01 | Axiogenesis Ag | Assay for drug discovery based on in vitro differentiated cells |
US20060292694A1 (en) * | 2005-06-22 | 2006-12-28 | Roslin Institute | Reporter hepatocytes and other cells for drug screening and toxicity testing |
EP1893749A1 (en) * | 2005-06-22 | 2008-03-05 | Geron Corporation | Reporter hepatocytes and other cells for drug screening and toxicity testing |
US20060292695A1 (en) * | 2005-06-22 | 2006-12-28 | Roslin Institute | Methods and kits for drug screening and toxicity testing using promoter-reporter cells derived from embryonic stem cells |
JP5860805B2 (ja) * | 2010-03-23 | 2016-02-16 | オリンパス株式会社 | 幹細胞の分化状態をモニタリングする方法 |
ITPI20100085A1 (it) | 2010-07-07 | 2012-01-08 | Sara Migliarini | Procedimento per l'ingegnerizzazione genetica di cellule staminali che attivano un tracciante fluorescente vitale in seguito al differenziamento in vitro delle cellule stesse verso il tipo cellulare specifico dei neuroni serotoninergici. |
JP2014176364A (ja) * | 2013-03-15 | 2014-09-25 | Olympus Corp | 幹細胞の解析方法 |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US6670449B1 (en) * | 1997-02-13 | 2003-12-30 | Memorial Sloan-Kettering Cancer Center | Hybrid molecules and their use for optically detecting changes in cellular microenvironments |
DE19727962A1 (de) * | 1997-07-02 | 1999-01-14 | Juergen Hescheler | Fluoreszierende Proteine als zelltypspezifische Reporter |
WO2002051987A1 (de) * | 2000-12-27 | 2002-07-04 | Axiogenesis Ag | System zur zell-und entwicklungsspezifischen selektion differenzierender embryonaler stammzellen; adulter stammzellen und embryonaler keinbahnzellen |
EP2578676A1 (en) * | 2003-06-20 | 2013-04-10 | Axiogenesis Ag | Tissue modeling in multi- or pluripotent cell systems |
WO2005005621A2 (en) * | 2003-07-08 | 2005-01-20 | Axiogenesis Ag | NOVEL METHOD FOR THE PREPARATION OF EMBRYOID BODIES (EBs) AND USES THEREOF |
-
1997
- 1997-07-02 DE DE19727962A patent/DE19727962A1/de not_active Ceased
-
1998
- 1998-06-30 WO PCT/EP1998/003988 patent/WO1999001552A1/de active IP Right Grant
- 1998-06-30 DK DK98940092T patent/DK1002080T3/da active
- 1998-06-30 DE DE59807479T patent/DE59807479D1/de not_active Expired - Lifetime
- 1998-06-30 ES ES98940092T patent/ES2191324T3/es not_active Expired - Lifetime
- 1998-06-30 PT PT98940092T patent/PT1002080E/pt unknown
- 1998-06-30 JP JP50631699A patent/JP3956154B2/ja not_active Expired - Lifetime
- 1998-06-30 EP EP98940092A patent/EP1002080B9/de not_active Expired - Lifetime
- 1998-06-30 AT AT98940092T patent/ATE234357T1/de active
-
2002
- 2002-02-28 US US10/084,960 patent/US7105344B2/en not_active Expired - Lifetime
-
2005
- 2005-12-05 US US11/293,932 patent/US20060168665A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
DE19727962A1 (de) | 1999-01-14 |
US20020092035A1 (en) | 2002-07-11 |
ATE234357T1 (de) | 2003-03-15 |
WO1999001552A1 (de) | 1999-01-14 |
JP2002508670A (ja) | 2002-03-19 |
PT1002080E (pt) | 2003-06-30 |
EP1002080B1 (de) | 2003-03-12 |
EP1002080B9 (de) | 2004-08-11 |
US20060168665A1 (en) | 2006-07-27 |
DK1002080T3 (da) | 2003-05-26 |
JP3956154B2 (ja) | 2007-08-08 |
EP1002080A1 (de) | 2000-05-24 |
US7105344B2 (en) | 2006-09-12 |
DE59807479D1 (de) | 2003-04-17 |
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