DK0843725T3 - Fremgangmåde til fremstilling af polypeptidvarianter - Google Patents

Fremgangmåde til fremstilling af polypeptidvarianter

Info

Publication number
DK0843725T3
DK0843725T3 DK96926325T DK96926325T DK0843725T3 DK 0843725 T3 DK0843725 T3 DK 0843725T3 DK 96926325 T DK96926325 T DK 96926325T DK 96926325 T DK96926325 T DK 96926325T DK 0843725 T3 DK0843725 T3 DK 0843725T3
Authority
DK
Denmark
Prior art keywords
polypeptide
dna
homologous
encoding
polypeptide variants
Prior art date
Application number
DK96926325T
Other languages
Danish (da)
English (en)
Inventor
Jens Sigurd Okkels
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of DK0843725T3 publication Critical patent/DK0843725T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DK96926325T 1995-08-11 1996-08-12 Fremgangmåde til fremstilling af polypeptidvarianter DK0843725T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DK90795 1995-08-11
DK104795 1995-09-20
PCT/DK1996/000343 WO1997007205A1 (en) 1995-08-11 1996-08-12 Method for preparing polypeptide variants

Publications (1)

Publication Number Publication Date
DK0843725T3 true DK0843725T3 (da) 2002-08-12

Family

ID=26064882

Family Applications (1)

Application Number Title Priority Date Filing Date
DK96926325T DK0843725T3 (da) 1995-08-11 1996-08-12 Fremgangmåde til fremstilling af polypeptidvarianter

Country Status (9)

Country Link
US (2) US20030148441A1 (zh)
EP (2) EP0843725B1 (zh)
JP (1) JP4156666B2 (zh)
CN (1) CN1128875C (zh)
AT (1) ATE216427T1 (zh)
AU (2) AU6655396A (zh)
DE (1) DE69620766T2 (zh)
DK (1) DK0843725T3 (zh)
WO (2) WO1997007206A1 (zh)

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Also Published As

Publication number Publication date
EP1213350A2 (en) 2002-06-12
US20050048649A1 (en) 2005-03-03
DE69620766T2 (de) 2004-11-18
AU6655396A (en) 1997-03-12
US20030148441A1 (en) 2003-08-07
US20050124066A9 (en) 2005-06-09
EP0843725A1 (en) 1998-05-27
CN1192782A (zh) 1998-09-09
JPH11510700A (ja) 1999-09-21
ATE216427T1 (de) 2002-05-15
CN1128875C (zh) 2003-11-26
EP0843725B1 (en) 2002-04-17
DE69620766D1 (de) 2002-05-23
WO1997007205A1 (en) 1997-02-27
EP1213350A3 (en) 2002-12-04
AU6655496A (en) 1997-03-12
JP4156666B2 (ja) 2008-09-24
WO1997007206A1 (en) 1997-02-27

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