DK0832200T3 - Nye metoder til rensning af faktor IX - Google Patents

Nye metoder til rensning af faktor IX

Info

Publication number
DK0832200T3
DK0832200T3 DK96915892T DK96915892T DK0832200T3 DK 0832200 T3 DK0832200 T3 DK 0832200T3 DK 96915892 T DK96915892 T DK 96915892T DK 96915892 T DK96915892 T DK 96915892T DK 0832200 T3 DK0832200 T3 DK 0832200T3
Authority
DK
Denmark
Prior art keywords
purification
factor
new methods
hydroxyapatite
resins
Prior art date
Application number
DK96915892T
Other languages
English (en)
Inventor
W Barry Foster
Robert J Costigan
Duane Bonam
Mary B Switzer
Rochelle Walsh
Original Assignee
Genetics Inst Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetics Inst Llc filed Critical Genetics Inst Llc
Application granted granted Critical
Publication of DK0832200T3 publication Critical patent/DK0832200T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21022—Coagulation factor IXa (3.4.21.22)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Compounds Of Unknown Constitution (AREA)
DK96915892T 1995-06-07 1996-05-21 Nye metoder til rensning af faktor IX DK0832200T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/472,823 US5714583A (en) 1995-06-07 1995-06-07 Factor IX purification methods
PCT/US1996/007305 WO1996040883A1 (en) 1995-06-07 1996-05-21 Novel factor ix purification methods

Publications (1)

Publication Number Publication Date
DK0832200T3 true DK0832200T3 (da) 2008-07-21

Family

ID=23877079

Family Applications (2)

Application Number Title Priority Date Filing Date
DK96915892T DK0832200T3 (da) 1995-06-07 1996-05-21 Nye metoder til rensning af faktor IX
DK05012614.3T DK1571208T3 (da) 1995-06-07 1996-05-21 Fremgangsmåde til ny faktor IX-rensning

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK05012614.3T DK1571208T3 (da) 1995-06-07 1996-05-21 Fremgangsmåde til ny faktor IX-rensning

Country Status (11)

Country Link
US (2) US5714583A (da)
EP (2) EP1571208B1 (da)
JP (1) JP3676817B2 (da)
AT (2) ATE488582T1 (da)
AU (1) AU5754396A (da)
CA (1) CA2220501C (da)
DE (2) DE69637509T2 (da)
DK (2) DK0832200T3 (da)
ES (2) ES2306450T3 (da)
PT (2) PT832200E (da)
WO (1) WO1996040883A1 (da)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5714583A (en) * 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods
US20030036629A1 (en) * 1997-12-12 2003-02-20 Barry Foster Novel tgf-beta protein purification methods
ATE370961T1 (de) 1998-05-06 2007-09-15 Genentech Inc Reinigung von antikörpern durch ionenaustauschchromatographie
IL142069A0 (en) 1998-09-21 2002-03-10 Genetics Inst Methods of downmodulating the immune response to therapeutic proteins
WO2002039951A2 (en) * 2000-11-15 2002-05-23 Globe Immune, Inc. Yeast-dentritic cell vaccines and uses thereof
KR100451308B1 (ko) * 2001-12-28 2004-10-06 선바이오(주) 단백질 의약품 정제 방법에서의 바이러스 제거 방법
DK1581644T3 (da) 2003-01-09 2007-10-08 Genentech Inc Oprensning af polypeptider
US20050008580A1 (en) * 2003-04-09 2005-01-13 Wyeth Hemophilia treatment by inhalation of coagulation factors
EP1765411B2 (en) * 2004-06-30 2017-10-11 Nektar Therapeutics Polymer-factor ix moiety conjugates
ES2503365T5 (es) 2005-12-21 2017-10-31 Cnj Holdings, Inc Método para producir proteínas dependientes de vitamina K biológicamente activas por métodos recombinantes
US7566565B2 (en) 2006-03-24 2009-07-28 Syntonix Pharmaceuticals, Inc. PC5 as a factor IX propeptide processing enzyme
WO2008104372A1 (en) * 2007-02-28 2008-09-04 Baxter International Inc. Method for the purification of recombinant blood coagulation factor ix enriched in sulfated and/or phosphorylated molecules
EP2517714A1 (en) 2007-04-26 2012-10-31 Inspiration Biopharmaceuticals, Inc. Recombinant vitamin K dependent proteins with high sialic acid content and methods of preparing same
HUE037409T2 (hu) 2007-10-30 2018-08-28 Genentech Inc Antitest-tisztítás kationcserés kromatográfiával
EP2326658A4 (en) * 2008-09-12 2013-04-10 Ge Healthcare Bio Sciences Ab IMPROVED PROTEIN-AGGREGATE SEPARATION WITH MULTIMODAL ANION EXCHANGERS IN THE PRESENCE OF ZWITTERIONS EXCLUDED FROM THE PROTEIN
FR2946348B1 (fr) 2009-06-05 2011-08-05 Lab Francais Du Fractionnement Procede de preparation d'une composition de complexe prothrombique a haut degre de purete
JP5781067B2 (ja) 2009-07-10 2015-09-16 シーエスエル、リミテッド ビタミンｋ依存性タンパク質の発現収率を増加させる方法
EP2494040B1 (en) 2009-10-30 2018-08-29 Aptevo BioTherapeutics LLC Method of producing recombinant vitamin k dependent proteins
US8697844B2 (en) 2009-11-24 2014-04-15 Novo Nordisk A/S Method of purifying pegylated proteins
JP5876868B2 (ja) * 2010-03-30 2016-03-02 オクタファルマ・アーゲー 第ｉｘ凝固因子などのビタミンｋ依存性タンパク質の精製方法
CA2793143C (en) 2010-03-30 2020-04-28 Gustav Gilljam Process for the purification of a growth factor protein
NZ603289A (en) * 2010-04-29 2014-07-25 Baxter Int Purification method for divalent cation binding proteins on anion exchange resin
US9488625B2 (en) 2010-12-15 2016-11-08 Baxalta GmbH Purification of factor VIII using a conductivity gradient
BRPI1105317A2 (pt) 2011-01-24 2013-04-30 Fundacco Hemoct De Ribeirco Preto produÇço estÁvel e em larga escala de fviii humano em linhagem celular humana sk-hep-1
JP2014525475A (ja) 2011-09-06 2014-09-29 メディミューン，エルエルシー 凝固因子を処理する方法
EP2748180B1 (en) * 2011-10-14 2018-08-15 Baxalta GmbH Protein purification by anion exchange chromatography
US10378004B2 (en) 2011-10-14 2019-08-13 Baxalta GmbH Protein purification by anion exchange chromatography
SI2970376T1 (sl) 2013-03-15 2018-09-28 Baxalta Incorporated Metoda čiščenja za vitamin k odvisne proteine z anionsko izmenjevalno kromatografijo
US9663553B2 (en) 2014-01-29 2017-05-30 Hemarus Therapeutics Limited Integrated process for the production of therapeutics (human albumin, immunoglobulins, clotting factor VIII and clotting factor IX) from human plasma
CN114134109B (zh) * 2021-12-10 2023-01-24 广州远想生物科技股份有限公司 Egf间充质干细胞外泌体的纯化方法

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3101752A1 (de) * 1981-01-21 1982-08-26 Behringwerke Ag, 3550 Marburg "verfahren zur reinigung der blutgerinnungsfaktoren ii, vii, ix und/oder x und danach hergestellte praeparationen"
US4447416A (en) * 1982-04-28 1984-05-08 American National Red Cross Plasma protein concentrates of reduced thrombogenicity and their use in clinical replacement therapy
US5055557A (en) * 1983-03-04 1991-10-08 Scripps Clinic & Research Foundation Ultrapurification of factor IX and other vitamin K-dependent proteins
DE3486350T2 (de) * 1983-10-28 1995-03-16 New England Medical Center Inc VERFAHREN ZUR ISOLIERUNG EINES PROTEINS AUS EINER DIESES PROTEIN ENTHALTENDEN MISCHUNG UNTER VERWENDUNG EINES KONFORMATIONSSPEZIFISCHEN ANTIKöRPERS.
US4721572A (en) * 1985-07-12 1988-01-26 Miles Laboratories, Inc. Purfication of blood clotting factors and other blood proteins on non-carbohydrate sulfated matrices
US4786726A (en) * 1986-01-06 1988-11-22 Blood Systems, Inc. Factor IX therapeutic blood product, means and methods of preparing same
US4725673A (en) * 1986-08-29 1988-02-16 Alpha Therapeutic Corporation Plasma fraction purification using silica resin bound to a ligand
US4831118A (en) * 1987-08-07 1989-05-16 Scripps Clinic And Research Foundation Flouroplastic immunoaffinity columns for purification of blood proteins
DE3877529T2 (de) * 1987-10-23 1996-11-07 Centre Regional De Transfusion Herstellung von hoch-gereingtem menschlichen Faktor IX sowie anderem Plasmaproteinkonzentrat und dessen therapeutische Verwendung.
WO1989005652A1 (en) * 1987-12-21 1989-06-29 American National Red Cross Pure factor ix product
GB8729822D0 (en) * 1987-12-22 1988-02-03 Central Blood Lab Authority Chemical process
CA1330302C (en) * 1988-01-18 1994-06-21 Miroslav Rybak Concentrates of coagulation factors ii, vii, ix and x, method of their preparation and use
DE3826792C1 (da) * 1988-08-06 1989-07-06 Biotest Pharma Gmbh, 6072 Dreieich, De
US4981952A (en) * 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
DE3914869C1 (da) * 1989-05-05 1990-08-09 Biotest Pharma Gmbh, 6072 Dreieich, De
AT402261B (de) * 1991-01-25 1997-03-25 Immuno Ag Komplex enthaltend den gerinnungsfaktor ix
IT1262899B (it) * 1992-03-27 1996-07-22 Sclavo Spa Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano
US5714583A (en) * 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods

Also Published As

Publication number Publication date
EP0832200A1 (en) 1998-04-01
US5714583A (en) 1998-02-03
ES2353798T3 (es) 2011-03-07
EP0832200B1 (en) 2008-04-23
JP3676817B2 (ja) 2005-07-27
AU5754396A (en) 1996-12-30
CA2220501A1 (en) 1996-12-19
EP1571208B1 (en) 2010-11-17
DK1571208T3 (da) 2011-01-24
DE69638291D1 (de) 2010-12-30
US6627737B1 (en) 2003-09-30
ES2306450T3 (es) 2008-11-01
ATE393217T1 (de) 2008-05-15
PT832200E (pt) 2008-07-17
DE69637509D1 (de) 2008-06-05
EP1571208A1 (en) 2005-09-07
DE69637509T2 (de) 2009-05-28
JPH11506603A (ja) 1999-06-15
WO1996040883A1 (en) 1996-12-19
PT1571208E (pt) 2011-01-04
ATE488582T1 (de) 2010-12-15
CA2220501C (en) 2014-01-07

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