ATE488582T1 - Verfahren zur reinigung von faktor ix - Google Patents

Verfahren zur reinigung von faktor ix

Info

Publication number
ATE488582T1
ATE488582T1 AT05012614T AT05012614T ATE488582T1 AT E488582 T1 ATE488582 T1 AT E488582T1 AT 05012614 T AT05012614 T AT 05012614T AT 05012614 T AT05012614 T AT 05012614T AT E488582 T1 ATE488582 T1 AT E488582T1
Authority
AT
Austria
Prior art keywords
purifying factor
factor
hydroxyapatite
resins
purification
Prior art date
Application number
AT05012614T
Other languages
English (en)
Inventor
Barry W Foster
Robert J Costigan
Duane Bonam
Mary B Switzer
Rochelle Walsh
Original Assignee
Genetics Inst Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetics Inst Llc filed Critical Genetics Inst Llc
Application granted granted Critical
Publication of ATE488582T1 publication Critical patent/ATE488582T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424—Serine endopeptidases (3.4.21)
    • C12N9/644—Coagulation factor IXa (3.4.21.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C12Y304/21022—Coagulation factor IXa (3.4.21.22)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT05012614T 1995-06-07 1996-05-21 Verfahren zur reinigung von faktor ix ATE488582T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/472,823 US5714583A (en) 1995-06-07 1995-06-07 Factor IX purification methods

Publications (1)

Publication Number Publication Date
ATE488582T1 true ATE488582T1 (de) 2010-12-15

Family

ID=23877079

Family Applications (2)

Application Number Title Priority Date Filing Date
AT96915892T ATE393217T1 (de) 1995-06-07 1996-05-21 Verfahren zur reinigung von faktor-ix
AT05012614T ATE488582T1 (de) 1995-06-07 1996-05-21 Verfahren zur reinigung von faktor ix

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT96915892T ATE393217T1 (de) 1995-06-07 1996-05-21 Verfahren zur reinigung von faktor-ix

Country Status (11)

Country Link
US (2) US5714583A (de)
EP (2) EP1571208B1 (de)
JP (1) JP3676817B2 (de)
AT (2) ATE393217T1 (de)
AU (1) AU5754396A (de)
CA (1) CA2220501C (de)
DE (2) DE69637509T2 (de)
DK (2) DK1571208T3 (de)
ES (2) ES2353798T3 (de)
PT (2) PT1571208E (de)
WO (1) WO1996040883A1 (de)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5714583A (en) * 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods
US20030036629A1 (en) * 1997-12-12 2003-02-20 Barry Foster Novel tgf-beta protein purification methods
AU760048B2 (en) 1998-05-06 2003-05-08 Genentech Inc. Protein purification by ion exchange chromatography
CN1331602A (zh) 1998-09-21 2002-01-16 遗传研究所有限公司 负调节针对医用蛋白的免疫反应的方法
US7083787B2 (en) * 2000-11-15 2006-08-01 Globeimmune, Inc. Yeast-dendritic cell vaccines and uses thereof
KR100451308B1 (ko) * 2001-12-28 2004-10-06 선바이오(주) 단백질 의약품 정제 방법에서의 바이러스 제거 방법
RU2337968C2 (ru) 2003-01-09 2008-11-10 Дженентек, Инк. Способ очистки гетерологичного полипептида
US20050008580A1 (en) * 2003-04-09 2005-01-13 Wyeth Hemophilia treatment by inhalation of coagulation factors
EP1765411B2 (de) * 2004-06-30 2017-10-11 Nektar Therapeutics Polymer-faktor-ix-konjugate
US20080045453A1 (en) 2005-12-21 2008-02-21 Drohan William N Method of producing biologically active vitamin K dependent proteins by recombinant methods
EP2650305B1 (de) 2006-03-24 2024-05-08 Bioverativ Therapeutics Inc. PC5 als Faktor-IX-Propeptid-verarbeitendes Enzym
WO2008104372A1 (en) * 2007-02-28 2008-09-04 Baxter International Inc. Method for the purification of recombinant blood coagulation factor ix enriched in sulfated and/or phosphorylated molecules
CA2683423C (en) * 2007-04-26 2020-10-27 Inspiration Biopharmaceuticals, Inc. Recombinant vitamin k dependent proteins with high sialic acid content and methods of preparing same
DK2215117T4 (en) 2007-10-30 2018-04-09 Genentech Inc ANTISTO PURIFICATION BY CATION CHANGE CHROMATOGRAPHY
EP2326658A4 (de) * 2008-09-12 2013-04-10 Ge Healthcare Bio Sciences Ab Entfernung verstärkter proteinaggregate mit multimodalen anionentauschern in der präsenz von zwitterionen ohne proteine
FR2946348B1 (fr) 2009-06-05 2011-08-05 Lab Francais Du Fractionnement Procede de preparation d'une composition de complexe prothrombique a haut degre de purete
CN102471794B (zh) 2009-07-10 2014-10-29 Csl有限公司 提高维生素k依赖性蛋白质的表达产量的方法
WO2011053738A1 (en) 2009-10-30 2011-05-05 Inspiration Biopharmaceuticals, Inc. Method of producing recombinant vitamin k dependent proteins
US8697844B2 (en) 2009-11-24 2014-04-15 Novo Nordisk A/S Method of purifying pegylated proteins
RU2731720C2 (ru) * 2010-03-30 2020-09-08 Октафарма Аг Способ очистки витамин к-зависимых белков, таких как коагуляционный фактор vii
MX2012011065A (es) 2010-03-30 2012-10-10 Octapharma Ag Proceso para purificacion de proteina de factor de crecimiento.
AU2011247567B2 (en) * 2010-04-29 2014-06-19 Takeda Pharmaceutical Company Limited Purification method for divalent cation binding proteins on anion exchange resin
WO2012082933A1 (en) 2010-12-15 2012-06-21 Baxter International, Inc. Eluate collection using conductivity gradient
BRPI1105317A2 (pt) 2011-01-24 2013-04-30 Fundacco Hemoct De Ribeirco Preto produÇço estÁvel e em larga escala de fviii humano em linhagem celular humana sk-hep-1
AU2012304763A1 (en) 2011-09-06 2014-03-06 Medimmune Llc Methods for processing coagulation factors
AU2012322948B2 (en) * 2011-10-14 2014-11-06 Takeda Pharmaceutical Company Limited Protein purification by anion exchange chromatography
WO2013053888A1 (en) * 2011-10-14 2013-04-18 Baxter International Inc. Protein purification by anion exchange chromatography
JP6571011B2 (ja) 2013-03-15 2019-09-04 バクスアルタ インコーポレイテッド 陰イオン交換クロマトグラフィーによるビタミンｋ依存性タンパク質の精製方法
US9663553B2 (en) 2014-01-29 2017-05-30 Hemarus Therapeutics Limited Integrated process for the production of therapeutics (human albumin, immunoglobulins, clotting factor VIII and clotting factor IX) from human plasma
CN114134109B (zh) * 2021-12-10 2023-01-24 广州远想生物科技股份有限公司 Egf间充质干细胞外泌体的纯化方法

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3101752A1 (de) * 1981-01-21 1982-08-26 Behringwerke Ag, 3550 Marburg "verfahren zur reinigung der blutgerinnungsfaktoren ii, vii, ix und/oder x und danach hergestellte praeparationen"
US4447416A (en) * 1982-04-28 1984-05-08 American National Red Cross Plasma protein concentrates of reduced thrombogenicity and their use in clinical replacement therapy
US5055557A (en) * 1983-03-04 1991-10-08 Scripps Clinic & Research Foundation Ultrapurification of factor IX and other vitamin K-dependent proteins
JPS61500226A (ja) * 1983-10-28 1986-02-06 ニュ−・イングランド・メディカル・センタ−・ホスピタルズ・インコ−ポレ−テッド コンホメ−ション特異性抗体を用いる血液凝固タンパク質の精製および単離法
US4721572A (en) * 1985-07-12 1988-01-26 Miles Laboratories, Inc. Purfication of blood clotting factors and other blood proteins on non-carbohydrate sulfated matrices
US4786726A (en) * 1986-01-06 1988-11-22 Blood Systems, Inc. Factor IX therapeutic blood product, means and methods of preparing same
US4725673A (en) * 1986-08-29 1988-02-16 Alpha Therapeutic Corporation Plasma fraction purification using silica resin bound to a ligand
US4831118A (en) * 1987-08-07 1989-05-16 Scripps Clinic And Research Foundation Flouroplastic immunoaffinity columns for purification of blood proteins
DE3877529T2 (de) * 1987-10-23 1996-11-07 Centre Regional De Transfusion Herstellung von hoch-gereingtem menschlichen Faktor IX sowie anderem Plasmaproteinkonzentrat und dessen therapeutische Verwendung.
WO1989005652A1 (en) * 1987-12-21 1989-06-29 American National Red Cross Pure factor ix product
GB8729822D0 (en) * 1987-12-22 1988-02-03 Central Blood Lab Authority Chemical process
US5118614A (en) * 1988-01-18 1992-06-02 Tessek Sdruzeni Praha Concentrates of coagulation factors ii, vii, ix and x, method of their preparation and use
DE3826792C1 (de) * 1988-08-06 1989-07-06 Biotest Pharma Gmbh, 6072 Dreieich, De
US4981952A (en) * 1988-10-04 1991-01-01 Eli Lilly And Company Method for the purification of vitamin K-dependent proteins
DE3914869C1 (de) * 1989-05-05 1990-08-09 Biotest Pharma Gmbh, 6072 Dreieich, De
AT402261B (de) * 1991-01-25 1997-03-25 Immuno Ag Komplex enthaltend den gerinnungsfaktor ix
IT1262899B (it) * 1992-03-27 1996-07-22 Sclavo Spa Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano
US5714583A (en) * 1995-06-07 1998-02-03 Genetics Institute, Inc. Factor IX purification methods

Also Published As

Publication number Publication date
WO1996040883A1 (en) 1996-12-19
DE69637509D1 (de) 2008-06-05
CA2220501C (en) 2014-01-07
JPH11506603A (ja) 1999-06-15
EP1571208A1 (de) 2005-09-07
EP1571208B1 (de) 2010-11-17
ES2353798T3 (es) 2011-03-07
JP3676817B2 (ja) 2005-07-27
DE69637509T2 (de) 2009-05-28
DK1571208T3 (da) 2011-01-24
ATE393217T1 (de) 2008-05-15
EP0832200A1 (de) 1998-04-01
EP0832200B1 (de) 2008-04-23
AU5754396A (en) 1996-12-30
PT832200E (pt) 2008-07-17
DE69638291D1 (de) 2010-12-30
US6627737B1 (en) 2003-09-30
DK0832200T3 (da) 2008-07-21
PT1571208E (pt) 2011-01-04
CA2220501A1 (en) 1996-12-19
ES2306450T3 (es) 2008-11-01
US5714583A (en) 1998-02-03

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